Status
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Gene information
General information
TC014417 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10236264..10241216
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014417
      iBeetle screen iB_05629

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014417 RA
        MAYFHYRCG ERITLLNDN CTAVRSDSD FDNGLVITA 
        EPLVNDVLF EIKIDRKVN SWSGSLEIG VVDCDPLHF 
        DFPACSSKI QATSWIMSG MSFFKSGNC VIERYGADL 
        DKLNQDDRI GLMRTSEGD LIFYVNGES QGVAAENIP 
        SIVYAIVDV YGKCVQVSI TSPALREHN NDDCLSGSS 
        VLAIENDIL NVTLGGDLS ELSMSSSNS LDIRMDMNV 
        SLSLPEECP RPDKLRFHD RCGSLVKLS NGNRTAERR 
        RPLDEFNNG VVMTHRPLR DSELFEIRI DRLVDKWSG 
        SIEMGITTH NPSTLVFPA TMTNMRSGT IMMSGCGIL 
        TNGKGTRRE YGDFNLDEL SEGDRVGMM RKPDGNLHY 
        FINGLDQGV AAQRVPSTV WGVIDLYGM TIKVSIVER 
        DEREEQNLM TRKNTRDQQ IPVPDPQPL PDYYDRLTF 
        HPNCGTHAQ VINNGRTAH RPNAADDFN NGVVLTGRP 
        LKTGELFEV RLDRVVTKW AGSIEIGVT THSPVDLEF 
        PFTMTNVRS GTWMMTGSG IMHNGTIVL EQYGVNLDR 
        LQVGDRVGV VRKENGLLH FFVNGVDQG AAASNVPEK 
        VFGVIDLYG QAVEASIID MYDYGSPDT INSSLSNTT 
        LYSDLRFHH VHGKNARIV NGGLTALRP RPLSEFNDA 
        IVFSSRPLR DGELFEVCL DNIVDRWSG SIELGVTAV 
        RPDDIELPG TATDLCRDT WMLSGSSIM ENGTTVKNN 
        YPCDLDTLD AGVRIGVVR SSDKTLEFY RDGVPQGPA 
        CIVPHSTVY AVVDLYGQC AQVSIPCVS PLVPIPTNL 
        DNCPRSDTS ISLQAVSVV QPAVQTDLH IFSDCHGKG 
        VCLSDGGRL AKRTKDLTG AVVCSATTL EREELFEVT 
        IVSLVEHLA GSIVIGVSE TPPGKSNIN IFEQCCYIT 
        GNELRFRNK TIQLFTPSL HWLNVGDRI GLLRTLDGA 
        LKIFINSEE LNLCLPPLP ETLYVVVDL KGSCNAVAV 
        TSHKSPLWS LNSARLQDS LELCNQEQI ELVRDPDVS 
        TIYEFHDNH GRNIEIVEG RTTARRVAS YNQGVVIAQ 
        PALEISSKV QIIIDQLET RWQSSIIVG VVSGPPERL 
        NLPVNALAF KAPCCIVAN DWISVNGVK SRSNFGQRL 
        SNLQVGDTV GVMLTLSGL KLLVNGIEE EALSFVFQT 
        GQAIYAVFD LYGQCQQIK ILNDVAQEV AENSVTDYE 
        KADLESCEK ERLTLPIPS ASTSLTSSG GVSRPSPPS 
        PIKSLICSY RDECLTFKK RLLLPDNYF TSDDPICHC 
        STCIKGEKD NSLLGWVKF PLKNSANTA TDKWHTAFY 
        VCKVGAVRC ILDKGQPLT KGQAQWCNL VAQKEDDVQ 
        VVFYPTLQN CKTSGYKTN NRVVHAAFQ LLVKPSAYS 
        VSRDPPEWS TKETGAIVL HSLLIQIEC N
      CDS sequence
      • >TC014417 RA
        atggcatat tttcactac cgttgcggc gaacgcatt 
        actttatta aacgacaat tgtacggcg gtgcgcagc 
        gacagtgac ttcgacaat ggtttggta ataacggcc 
        gaacccctc gtcaacgat gtccttttc gaaataaaa 
        atcgacaga aaagtcaat tcctggtca gggagcctc 
        gaaattggc gtggttgac tgtgacccc ttgcatttc 
        gattttccg gcatgctct tcaaaaata caagccacc 
        tcatggatc atgtcagga atgtcgttc ttcaagagc 
        ggaaattgt gtaattgag aggtatggc gccgatttg 
        gataaatta aatcaggat gataggata ggtttaatg 
        cgcacatct gagggtgat ttaattttc tacgtcaac 
        ggggagtcc caaggagtc gccgctgaa aacatccca 
        agtatagtc tatgccata gtcgatgtt tacggcaaa 
        tgcgtccaa gtcagtatt actagtccc gccctccgg 
        gaacacaac aacgacgac tgcctcagc ggcagttcg 
        gtcctggcc atagaaaac gatatctta aatgtgacc 
        ctagggggc gacttaagc gaactcagc atgagcagt 
        agtaacagt cttgatatc cgtatggat atgaacgtg 
        agtctgagt ctcccagag gagtgcccc cgaccagat 
        aagttacgt ttccacgat cgttgcggc tcattggtc 
        aaactatca aacggcaat cggacagct gaacgtcgc 
        cggccttta gacgagttc aacaacggg gtggtcatg 
        acccaccgc cccctacgc gactcggag ctgttcgaa 
        atccgaatt gaccgcctc gtcgacaag tggagcggc 
        agtatcgag atgggcatc actacgcac aaccccagc 
        acgctggta ttccccgcc acgatgacc aacatgcgc 
        agcggcacc ataatgatg tccggttgt ggcatccta 
        acgaacgga aaaggcacc agacgcgag tacggcgac 
        ttcaacctc gacgagttg agcgaaggc gatcgcgtc 
        ggcatgatg cgcaagccc gacggcaat ttacactac 
        ttcataaat gggctggac cagggggtg gccgcccaa 
        cgcgtccct tccaccgtt tggggcgtg atcgacctc 
        tacggcatg acgatcaaa gtatcgatc gttgaacgc 
        gacgaaaga gaagagcag aatctcatg acgcgtaaa 
        aacacccgc gaccaacaa atcccagtc cctgatccc 
        caacctctt cccgactac tatgaccgg ttgaccttt 
        catccgaat tgcggtacg catgcgcaa gttattaat 
        aatggcagg actgcccat aggccgaac gcagccgat 
        gattttaat aacggggtg gtgcttacg gggcggcct 
        ttaaaaacg ggggagttg ttcgaggtg aggctggat 
        agggtggtg acgaagtgg gcggggtcg attgaaata 
        ggcgtgacg acgcacagt ccggttgat ttggagttc 
        ccgtttact atgacgaat gtgaggtcg gggacgtgg 
        atgatgacg ggaagtggg attatgcac aatgggacg 
        atcgttttg gagcaatat ggggtcaat ttggacagg 
        ttgcaagtg ggggatcgc gtgggcgtg gtcaggaag 
        gaaaatggg ttgttacac ttttttgtc aatggggtg 
        gatcaaggg gcggccgct tcgaacgtc cccgagaaa 
        gttttcgga gtcatagat ttatacggc caggctgtt 
        gaggcttca atcatcgac atgtacgac tatggatca 
        cccgataca attaactcg agtttatcc aacacaaca 
        ctttacagt gatttgagg tttcaccac gttcatggc 
        aaaaacgcc cggattgtg aacgggggc ttgaccgcg 
        ttaaggcct cgccccctt tcggaattc aatgatgct 
        attgtgttt tcgagtcgg ccgcttcgc gacggcgaa 
        cttttcgag gtttgtctc gacaatatc gtcgatcgg 
        tggtcggga agcatcgaa ttaggtgtg accgccgtt 
        cgccccgac gatatcgag cttccgggc accgccacc 
        gacctctgt cgcgacacc tggatgctc agcggatcc 
        tccattatg gaaaatggc accacggtc aaaaacaac 
        tacccctgc gacttggac actctcgac gctggcgtt 
        cgaattgga gtcgtgcgt tcaagcgac aaaactttg 
        gagttttac agagacggt gtcccccaa ggacccgcc 
        tgtattgtc ccccacagt acggtatat gccgttgtc 
        gatctctac gggcaatgt gcccaagtt agcattcct 
        tgtgtctcc ccacttgtt cctattccc acaaattta 
        gataactgt ccccggtcg gacacttcc attagtttg 
        caagcggtt agtgtagtc caaccggcg gttcaaacc 
        gatctacat atcttctct gattgtcac ggcaaaggc 
        gtgtgtttg agtgacggt ggccgattg gccaaacgc 
        acgaaagat ttaaccggg gcggtggta tgtagcgcc 
        acaacgctc gaaagggag gagctattc gaggtcact 
        atagtttca ttggtcgag catttggcc ggaagtata 
        gtcataggc gtgtcggaa acacctccc gggaaatcc 
        aacattaat atcttcgaa caatgttgt tacatcacc 
        gggaacgaa ctcagattt agaaacaaa acaattcag 
        ttatttact ccgagcttg cattggctg aacgtcggg 
        gaccgaatc gggttactc cgaacgctc gatggcgct 
        ttaaaaatt ttcataaac agtgaagaa ttaaacttg 
        tgtttgccc cctctccct gagacttta tacgtggtt 
        gtagacctg aaagggtcg tgtaatgcc gtcgccgtg 
        accagtcac aagtcaccc ttgtggtcc ctaaacagc 
        gcacggttg caagacagt ctcgaactt tgcaaccaa 
        gaacaaatc gaactggtc agagatccg gacgtgtca 
        acaatttac gaatttcac gacaaccac ggacgaaat 
        atcgagatt gttgagggt cgtacgact gcgagacgt 
        gtcgcttca tataatcag ggcgtggtg atcgctcag 
        cccgccctc gagatcagc tcaaaggtc cagatcata 
        atcgaccaa ctggagacg cgatggcaa tcgtcgatt 
        atcgtgggc gtggtttcg ggaccacct gaacgtttg 
        aatctacct gtcaatgca ctggcgttc aaagcaccg 
        tgttgcatt gtcgcaaac gattggatt tcggtgaac 
        ggggtcaag tcacgttcg aacttcggc caacgtttg 
        agtaattta caagttggc gatactgtg ggtgttatg 
        ttgaccctt tccgggttg aagttgtta gtgaatggg 
        attgaggag gaagctttg tcttttgtt tttcaaacg 
        gggcaagcc atttacgct gtttttgat ctctacggg 
        cagtgccaa cagattaaa atcttgaat gatgttgcg 
        caagaggtg gccgaaaat tcggtcact gattacgaa 
        aaggcggac cttgaaagt tgcgaaaag gagcgactg 
        actttaccc ataccctca gcaagtact agtctaaca 
        tcgtcaggg ggcgtgtcg cgtccctca ccgcctagt 
        ccaatcaaa tcgctgatt tgttcctac agggacgag 
        tgtctcacg tttaagaaa cgcctcctt ttgccagat 
        aattatttt acgtcagac gacccgatc tgccactgc 
        tcgacttgc ataaaaggc gagaaagat aactctctc 
        ctgggttgg gtgaaattt cctctgaaa aattcagcc 
        aatacagcg accgataag tggcacacg gccttctac 
        gtgtgtaag gtgggggcg gtgcgttgt atactcgac 
        aaagggcag ccgctgacc aaaggccag gcccagtgg 
        tgtaatcta gtggcacag aaagaagac gatgtccaa 
        gtcgttttt tacccaacc ttacaaaac tgcaagact 
        tcagggtac aagacaaac aatcgagtg gtccatgcc 
        gcttttcaa ttgttagtc aaaccgagc gcctactcc 
        gtatcgagg gatccaccc gaatggagc accaaagaa 
        accggagca attgttcta cattcgctt ttgattcaa 
        attgaatgt aattaa
      mRNA sequence
      • >TC014417 RA
        tgcttcaaa tttgagcca gcaaacggg cacacaatg 
        gcatatttt cactaccgt tgcggcgaa cgcattact 
        ttattaaac gacaattgt acggcggtg cgcagcgac 
        agtgacttc gacaatggt ttggtaata acggccgaa 
        cccctcgtc aacgatgtc cttttcgaa ataaaaatc 
        gacagaaaa gtcaattcc tggtcaggg agcctcgaa 
        attggcgtg gttgactgt gaccccttg catttcgat 
        tttccggca tgctcttca aaaatacaa gccacctca 
        tggatcatg tcaggaatg tcgttcttc aagagcgga 
        aattgtgta attgagagg tatggcgcc gatttggat 
        aaattaaat caggatgat aggataggt ttaatgcgc 
        acatctgag ggtgattta attttctac gtcaacggg 
        gagtcccaa ggagtcgcc gctgaaaac atcccaagt 
        atagtctat gccatagtc gatgtttac ggcaaatgc 
        gtccaagtc agtattact agtcccgcc ctccgggaa 
        cacaacaac gacgactgc ctcagcggc agttcggtc 
        ctggccata gaaaacgat atcttaaat gtgacccta 
        gggggcgac ttaagcgaa ctcagcatg agcagtagt 
        aacagtctt gatatccgt atggatatg aacgtgagt 
        ctgagtctc ccagaggag tgcccccga ccagataag 
        ttacgtttc cacgatcgt tgcggctca ttggtcaaa 
        ctatcaaac ggcaatcgg acagctgaa cgtcgccgg 
        cctttagac gagttcaac aacggggtg gtcatgacc 
        caccgcccc ctacgcgac tcggagctg ttcgaaatc 
        cgaattgac cgcctcgtc gacaagtgg agcggcagt 
        atcgagatg ggcatcact acgcacaac cccagcacg 
        ctggtattc cccgccacg atgaccaac atgcgcagc 
        ggcaccata atgatgtcc ggttgtggc atcctaacg 
        aacggaaaa ggcaccaga cgcgagtac ggcgacttc 
        aacctcgac gagttgagc gaaggcgat cgcgtcggc 
        atgatgcgc aagcccgac ggcaattta cactacttc 
        ataaatggg ctggaccag ggggtggcc gcccaacgc 
        gtcccttcc accgtttgg ggcgtgatc gacctctac 
        ggcatgacg atcaaagta tcgatcgtt gaacgcgac 
        gaaagagaa gagcagaat ctcatgacg cgtaaaaac 
        acccgcgac caacaaatc ccagtccct gatccccaa 
        cctcttccc gactactat gaccggttg acctttcat 
        ccgaattgc ggtacgcat gcgcaagtt attaataat 
        ggcaggact gcccatagg ccgaacgca gccgatgat 
        tttaataac ggggtggtg cttacgggg cggccttta 
        aaaacgggg gagttgttc gaggtgagg ctggatagg 
        gtggtgacg aagtgggcg gggtcgatt gaaataggc 
        gtgacgacg cacagtccg gttgatttg gagttcccg 
        tttactatg acgaatgtg aggtcgggg acgtggatg 
        atgacggga agtgggatt atgcacaat gggacgatc 
        gttttggag caatatggg gtcaatttg gacaggttg 
        caagtgggg gatcgcgtg ggcgtggtc aggaaggaa 
        aatgggttg ttacacttt tttgtcaat ggggtggat 
        caaggggcg gccgcttcg aacgtcccc gagaaagtt 
        ttcggagtc atagattta tacggccag gctgttgag 
        gcttcaatc atcgacatg tacgactat ggatcaccc 
        gatacaatt aactcgagt ttatccaac acaacactt 
        tacagtgat ttgaggttt caccacgtt catggcaaa 
        aacgcccgg attgtgaac gggggcttg accgcgtta 
        aggcctcgc cccctttcg gaattcaat gatgctatt 
        gtgttttcg agtcggccg cttcgcgac ggcgaactt 
        ttcgaggtt tgtctcgac aatatcgtc gatcggtgg 
        tcgggaagc atcgaatta ggtgtgacc gccgttcgc 
        cccgacgat atcgagctt ccgggcacc gccaccgac 
        ctctgtcgc gacacctgg atgctcagc ggatcctcc 
        attatggaa aatggcacc acggtcaaa aacaactac 
        ccctgcgac ttggacact ctcgacgct ggcgttcga 
        attggagtc gtgcgttca agcgacaaa actttggag 
        ttttacaga gacggtgtc ccccaagga cccgcctgt 
        attgtcccc cacagtacg gtatatgcc gttgtcgat 
        ctctacggg caatgtgcc caagttagc attccttgt 
        gtctcccca cttgttcct attcccaca aatttagat 
        aactgtccc cggtcggac acttccatt agtttgcaa 
        gcggttagt gtagtccaa ccggcggtt caaaccgat 
        ctacatatc ttctctgat tgtcacggc aaaggcgtg 
        tgtttgagt gacggtggc cgattggcc aaacgcacg 
        aaagattta accggggcg gtggtatgt agcgccaca 
        acgctcgaa agggaggag ctattcgag gtcactata 
        gtttcattg gtcgagcat ttggccgga agtatagtc 
        ataggcgtg tcggaaaca cctcccggg aaatccaac 
        attaatatc ttcgaacaa tgttgttac atcaccggg 
        aacgaactc agatttaga aacaaaaca attcagtta 
        tttactccg agcttgcat tggctgaac gtcggggac 
        cgaatcggg ttactccga acgctcgat ggcgcttta 
        aaaattttc ataaacagt gaagaatta aacttgtgt 
        ttgccccct ctccctgag actttatac gtggttgta 
        gacctgaaa gggtcgtgt aatgccgtc gccgtgacc 
        agtcacaag tcacccttg tggtcccta aacagcgca 
        cggttgcaa gacagtctc gaactttgc aaccaagaa 
        caaatcgaa ctggtcaga gatccggac gtgtcaaca 
        atttacgaa tttcacgac aaccacgga cgaaatatc 
        gagattgtt gagggtcgt acgactgcg agacgtgtc 
        gcttcatat aatcagggc gtggtgatc gctcagccc 
        gccctcgag atcagctca aaggtccag atcataatc 
        gaccaactg gagacgcga tggcaatcg tcgattatc 
        gtgggcgtg gtttcggga ccacctgaa cgtttgaat 
        ctacctgtc aatgcactg gcgttcaaa gcaccgtgt 
        tgcattgtc gcaaacgat tggatttcg gtgaacggg 
        gtcaagtca cgttcgaac ttcggccaa cgtttgagt 
        aatttacaa gttggcgat actgtgggt gttatgttg 
        accctttcc gggttgaag ttgttagtg aatgggatt 
        gaggaggaa gctttgtct tttgttttt caaacgggg 
        caagccatt tacgctgtt tttgatctc tacgggcag 
        tgccaacag attaaaatc ttgaatgat gttgcgcaa 
        gaggtggcc gaaaattcg gtcactgat tacgaaaag 
        gcggacctt gaaagttgc gaaaaggag cgactgact 
        ttacccata ccctcagca agtactagt ctaacatcg 
        tcagggggc gtgtcgcgt ccctcaccg cctagtcca 
        atcaaatcg ctgatttgt tcctacagg gacgagtgt 
        ctcacgttt aagaaacgc ctccttttg ccagataat 
        tattttacg tcagacgac ccgatctgc cactgctcg 
        acttgcata aaaggcgag aaagataac tctctcctg 
        ggttgggtg aaatttcct ctgaaaaat tcagccaat 
        acagcgacc gataagtgg cacacggcc ttctacgtg 
        tgtaaggtg ggggcggtg cgttgtata ctcgacaaa 
        gggcagccg ctgaccaaa ggccaggcc cagtggtgt 
        aatctagtg gcacagaaa gaagacgat gtccaagtc 
        gttttttac ccaacctta caaaactgc aagacttca 
        gggtacaag acaaacaat cgagtggtc catgccgct 
        tttcaattg ttagtcaaa ccgagcgcc tactccgta 
        tcgagggat ccacccgaa tggagcacc aaagaaacc 
        ggagcaatt gttctacat tcgcttttg attcaaatt 
        gaatgtaat taaatgtgt agcgattgg tgtgtgcaa 
        ttaaacatt cggaatttt ttttgtcga gctgtgatt 
        atgttaacg aatttagtc tgatgattg gaacaagtc 
        gaagatgtg atattattt ttcccaaat tcgtcatca 
        aactaattt tagaatagg ttaccgatg tatttattt 
        acaacaagc agattttat ccaagacac acggcactg 
        tagtatacg ttaagtttg tttacggac cgaataaaa 
        catgttgac caca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY