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Gene information
General information
TC014407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10176782..10178518
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014407
    iBeetle screen iB_05627

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014407 RA
      MASRLVRSL SITNRLVGS FKYKPTVMS RCCSTTPAD 
      KVLTIQELT VNLAKAMGV DSGFSPIRA DRSHLSEYL 
      PTSQSELPP RAMRDSYLV AMIPLSTST RLQDRYTTF 
      LGYVRVGRL LEDMDIFAV MVAQKHILN PKLPEGTPS 
      PQTLVTALV DRIDFSDFV PKPHEDIKI SGHVSWVGR 
      SSMEVVVWL EQKMYGTWH RITRALFLI AARDPTNTK 
      AAMVNAIEP ADDREKAIL AGGETRKAN RIALEKQHV 
      LKVIPDHDE QRIIHDLYL KTKSNKNLS FTNMVLPAN 
      GVWMEDCTI SNTIFSQPE NRNLHNTVF GGFIMRQAA 
      ELSWVLGFK FSKYRPKVK SISDIKFNK PIAVNSLIH 
      MYAHVVFTQ MQFIQIVVY VEVFDPQTG NTDTTNVVH 
      LTYEVPEIV REVYPRTYQ EAMMYIDGR RHFFEIMKT 
      PYDHDNVLN EEGIRNKSK L
    CDS sequence
    • >TC014407 RA
      atggcctct agattagta cgtagttta tcaatcacg 
      aaccgctta gtgggttca tttaaatac aaacctact 
      gtcatgagt cgctgctgt tccaccacg ccagccgac 
      aaggtgtta acaatacaa gaactaacc gtaaatttg 
      gcgaaagct atgggggtc gatagtggg ttttccccc 
      atccgggcc gaccgatct cacctctcc gaatacctc 
      cctacaagc caaagcgaa ctaccgcca cgagctatg 
      cgtgatagt tatctcgtg gccatgata cccctttcg 
      accagtacg cggctccaa gaccggtat acaacgttt 
      ctggggtac gtccgggtc gggcgctta ctcgaagac 
      atggatatt tttgccgtg atggtggcg cagaaacat 
      atacttaac ccgaaactg cccgaaggg acaccctct 
      ccccaaact ctagtcacc gcactggtg gacagaatc 
      gactttagc gactttgtc cctaaacct catgaggat 
      attaagatt tcggggcac gtcagttgg gtggggcgc 
      tcctccatg gaggtggtg gtgtggctg gagcagaaa 
      atgtacggg acgtggcac agaatcacg agggctttg 
      tttctgata gcagccagg gatccgacc aatacgaag 
      gcagcaatg gtcaatgcg atagagccg gcggatgac 
      cgcgaaaag gctatactt gctgggggg gaaacccgc 
      aaggctaat agaatagcc ttagagaag caacacgtg 
      ttgaaagtg ataccggat catgacgaa cagaggatc 
      atccacgat ttgtatttg aaaaccaag tccaataaa 
      aatctaagt ttcaccaac atggtactt cccgcgaat 
      ggggtctgg atggaagac tgtaccatc tcaaatacg 
      attttttcg cagccggaa aaccggaac ctccacaac 
      acggttttt ggagggttt atcatgaga caagcggcg 
      gaacttagc tgggttctt gggttcaaa tttagtaaa 
      tacaggcct aaagtcaag agtattagc gatattaag 
      tttaataaa ccaatagct gtgaattct cttattcat 
      atgtatgcc catgtggtc tttactcaa atgcaattt 
      atccaaatt gtggtctat gtggaagtt tttgatcca 
      cagacggga aatactgac accacgaat gttgtacat 
      ttaacttat gaagtgcct gagattgtc agggaggtg 
      tatcctcgg acttaccaa gaagccatg atgtatatt 
      gacggcaga agacatttc ttcgaaatt atgaagact 
      ccatatgac catgataac gtgttaaat gaagaagga 
      atacgcaat aaaagcaag ctttaa
    mRNA sequence
    • >TC014407 RA
      ttacgttta caaaatgac gcaataaac ttttttgca 
      acaattttg cttgtcact ttttgcagt attattaaa 
      ataatggcc tctagatta gtacgtagt ttatcaatc 
      acgaaccgc ttagtgggt tcatttaaa tacaaacct 
      actgtcatg agtcgctgc tgttccacc acgccagcc 
      gacaaggtg ttaacaata caagaacta accgtaaat 
      ttggcgaaa gctatgggg gtcgatagt gggttttcc 
      cccatccgg gccgaccga tctcacctc tccgaatac 
      ctccctaca agccaaagc gaactaccg ccacgagct 
      atgcgtgat agttatctc gtggccatg atacccctt 
      tcgaccagt acgcggctc caagaccgg tatacaacg 
      tttctgggg tacgtccgg gtcgggcgc ttactcgaa 
      gacatggat atttttgcc gtgatggtg gcgcagaaa 
      catatactt aacccgaaa ctgcccgaa gggacaccc 
      tctccccaa actctagtc accgcactg gtggacaga 
      atcgacttt agcgacttt gtccctaaa cctcatgag 
      gatattaag atttcgggg cacgtcagt tgggtgggg 
      cgctcctcc atggaggtg gtggtgtgg ctggagcag 
      aaaatgtac gggacgtgg cacagaatc acgagggct 
      ttgtttctg atagcagcc agggatccg accaatacg 
      aaggcagca atggtcaat gcgatagag ccggcggat 
      gaccgcgaa aaggctata cttgctggg ggggaaacc 
      cgcaaggct aatagaata gccttagag aagcaacac 
      gtgttgaaa gtgataccg gatcatgac gaacagagg 
      atcatccac gatttgtat ttgaaaacc aagtccaat 
      aaaaatcta agtttcacc aacatggta cttcccgcg 
      aatggggtc tggatggaa gactgtacc atctcaaat 
      acgattttt tcgcagccg gaaaaccgg aacctccac 
      aacacggtt tttggaggg tttatcatg agacaagcg 
      gcggaactt agctgggtt cttgggttc aaatttagt 
      aaatacagg cctaaagtc aagagtatt agcgatatt 
      aagtttaat aaaccaata gctgtgaat tctcttatt 
      catatgtat gcccatgtg gtctttact caaatgcaa 
      tttatccaa attgtggtc tatgtggaa gtttttgat 
      ccacagacg ggaaatact gacaccacg aatgttgta 
      catttaact tatgaagtg cctgagatt gtcagggag 
      gtgtatcct cggacttac caagaagcc atgatgtat 
      attgacggc agaagacat ttcttcgaa attatgaag 
      actccatat gaccatgat aacgtgtta aatgaagaa 
      ggaatacgc aataaaagc aagctttaa aagggttgg 
      gttcttggg tatttagtt taaatcgca atatgtcat 
      aattaactc gctttaaat ttccttgtt caattccat 
      gcgcaatca tatgtgata ttaacagtt ttactaatt 
      ttaattgta actttataa agttgatcg ttcttaatt 
      gatttgttt cctttttta tttttattt atttattta 
      tttaataaa tattgctgg ataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY