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Gene information
General information
TC014402 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10144172..10150381
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014402
    iBeetle screen iB_05626

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    vitellogenic egg chamber late partiallystructure irregular - Number of animals showing the phenotype: 1

    previtellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late slightlynumber decreased - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014402 RA
      MTGAWAACL LVILLPSCI PSPHRGRHH PPEPHAEAY 
      HMSRDPFDP HRDSEEFRR DAPDDKREF TRRDSEDDP 
      LVIQTKKGK VRGISLTAA TGKKVDAWL GIPYAQKPL 
      GNLRFRHPR PAEKWEGVM NTTSQPNSC VQIIDTVFG 
      DFPGATMWN PNTPLNEDC LYVNVVVPK PRPTSAAVM 
      VWVFGGGFY SGTNTLEVY DHNILVSEE NIILVSMQY 
      RVASLGFLY FGTPDVPGN AGLFDQMMA LQWVRDNIA 
      AFGGNPNNI TLFGESAGA VSVSLHLLS PLSRNLFSQ 
      AIMESGSAT APWAIISRE ESILRGLRL AEAVGCPHE 
      RHELSAVID CLKKKDPID LVNNEWGTL GICEFPFVP 
      VIDGAFLDE SPTRALANK NFKKTNILM GSNTEEGYY 
      FIIYYLTEL FRKEENVYV NRQEFLRAV TELNPYFNA 
      ISRQAIVFE YTNWLNPDD PVSNRDSLD KMVGDYHFT 
      CNVNEFAHR YAETGNTVY MYYYKHRTV ANPWPSWTG 
      VMHADEINY VFGEPLNPT KSYTAQEVD LSKRIMRYW 
      ANFAKTGNP SQSPNGVWT PTFWPPHTA FGREFLTLD 
      VNSTATGRG PRLKQCAFW KKYLPQLQQ QTSELLNQP 
      PRQNCTDAA SSLRWSRDG AAGLLMVST VAALLAGPF
    CDS sequence
    • >TC014402 RA
      atgacggga gcgtgggcg gcctgctta ctagtgatt 
      ctacttccc tcatgcatc ccttcgcca caccgaggc 
      cgccaccac ccccccgag cctcacgcc gaggcctac 
      cacatgtcc cgcgacccc ttcgacccg caccgggac 
      tcggaggag ttccgacga gacgccccg gacgacaag 
      cgggagttt acgcgtcgc gactccgaa gatgatccc 
      ctggtcatc cagacaaag aaagggaaa gtccgcggc 
      atctccctc acggccgcc acgggaaag aaggtcgac 
      gcgtggctg ggaatcccc tacgcgcag aaaccgctc 
      ggcaacctc cggttccgg cacccgcgg cctgcggaa 
      aagtgggag ggcgtgatg aacacgact agccagccg 
      aattcctgc gtgcagatt atagacaca gtgtttggg 
      gatttccca ggtgcgacc atgtggaac cccaacaca 
      ccgttgaac gaagactgt ctttacgtg aacgtggtg 
      gtgccgaag ccgaggccg accagtgcc gcggtcatg 
      gtgtgggtg ttcgggggt gggttctac tccgggacg 
      aacacttta gaagtttac gaccacaac atactagtg 
      tccgaagag aacattatt ttagtttcg atgcagtat 
      agagttgct tcgctcgga ttcctgtat ttcgggacg 
      ccggacgtt ccgggaaat gcaggattg tttgaccaa 
      atgatggcg ttgcaatgg gtacgtgat aacatcgcc 
      gctttcgga ggaaacccc aacaatatc actctattc 
      ggagaatca gcaggagct gtttcagtc tccctgcat 
      ctactctct ccattatcg agaaaccta ttctcacaa 
      gctatcatg gaatctggg agtgcgaca gcaccttgg 
      gctataatc tcccgtgaa gaaagcatt ttgcgagga 
      ttgagacta gcagaagct gtgggttgt ccgcatgag 
      cgccacgag ctttccgct gttatagac tgcttaaaa 
      aagaaagac cctattgat ttagtcaat aacgaatgg 
      ggaacactc ggcatatgt gagtttccg ttcgtccca 
      gtcattgat ggagccttt ctggacgaa tcccccaca 
      cgggctctg gcaaacaaa aacttcaaa aaaaccaat 
      atcctcatg ggttctaac acagaagaa ggctactac 
      ttcataatc tactacttg accgaactg ttccgaaag 
      gaggagaac gtttacgtc aaccggcag gaattcctg 
      cgggctgta accgaactg aacccgtac ttcaacgcg 
      atttcgcgc caagcaatc gtcttcgaa tacaccaac 
      tggttaaac cccgacgac ccggtgagc aaccgcgac 
      tccctcgat aaaatggta ggcgactac cacttcact 
      tgcaacgtc aacgaattc gcccaccgg tacgccgaa 
      accggcaac acggtctac atgtactac tacaagcac 
      cggacagtg gcgaatccc tggccctcc tggaccggc 
      gtgatgcac gccgacgaa atcaactac gtcttcggg 
      gaaccactc aatcccact aaaagctac acagcacaa 
      gaagtcgat ctgagtaag cgaatcatg agatactgg 
      gccaatttc gccaagacg ggcaacccg agtcagtcg 
      ccgaacggc gtttggaca cccactttc tggcccccg 
      cacacagct ttcggaagg gagtttctc accctcgat 
      gtcaactcc actgccaca gggagggga cctagactc 
      aagcagtgt gccttctgg aagaaatac ctacctcaa 
      cttcagcag caaacgagc gagcttctg aaccaaccg 
      ccccggcaa aactgtacc gacgcggcc agttcgttg 
      cgatggtca agagacggc gctgccggc ctcctgatg 
      gtgtcgaca gtggcggcg ctcctcgcg gggcctttc 
      taa
    mRNA sequence
    • >TC014402 RA
      accgcatgc tgtgtattg ttgacattt attcaaatt 
      attgaaaag cgatgacgg gagcgtggg cggcctgct 
      tactagtga ttctacttc cctcatgca tcccttcgc 
      cacaccgag gccgccacc acccccccg agcctcacg 
      ccgaggcct accacatgt cccgcgacc ccttcgacc 
      cgcaccggg actcggagg agttccgac gagacgccc 
      cggacgaca agcgggagt ttacgcgtc gcgactccg 
      aagatgatc ccctggtca tccagacaa agaaaggga 
      aagtccgcg gcatctccc tcacggccg ccacgggaa 
      agaaggtcg acgcgtggc tgggaatcc cctacgcgc 
      agaaaccgc tcggcaacc tccggttcc ggcacccgc 
      ggcctgcgg aaaagtggg agggcgtga tgaacacga 
      ctagccagc cgaattcct gcgtgcaga ttatagaca 
      cagtgtttg gggatttcc caggtgcga ccatgtgga 
      accccaaca caccgttga acgaagact gtctttacg 
      tgaacgtgg tggtgccga agccgaggc cgaccagtg 
      ccgcggtca tggtgtggg tgttcgggg gtgggttct 
      actccggga cgaacactt tagaagttt acgaccaca 
      acatactag tgtccgaag agaacatta ttttagttt 
      cgatgcagt atagagttg cttcgctcg gattcctgt 
      atttcggga cgccggacg ttccgggaa atgcaggat 
      tgtttgacc aaatgatgg cgttgcaat gggtacgtg 
      ataacatcg ccgctttcg gaggaaacc ccaacaata 
      tcactctat tcggagaat cagcaggag ctgtttcag 
      tctccctgc atctactct ctccattat cgagaaacc 
      tattctcac aagctatca tggaatctg ggagtgcga 
      cagcacctt gggctataa tctcccgtg aagaaagca 
      ttttgcgag gattgagac tagcagaag ctgtgggtt 
      gtccgcatg agcgccacg agctttccg ctgttatag 
      actgcttaa aaaagaaag accctattg atttagtca 
      ataacgaat ggggaacac tcggcatat gtgagtttc 
      cgttcgtcc cagtcattg atggagcct ttctggacg 
      aatccccca cacgggctc tggcaaaca aaaacttca 
      aaaaaacca atatcctca tgggttcta acacagaag 
      aaggctact acttcataa tctactact tgaccgaac 
      tgttccgaa aggaggaga acgtttacg tcaaccggc 
      aggaattcc tgcgggctg taaccgaac tgaacccgt 
      acttcaacg cgatttcgc gccaagcaa tcgtcttcg 
      aatacacca actggttaa accccgacg acccggtga 
      gcaaccgcg actccctcg ataaaatgg taggcgact 
      accacttca cttgcaacg tcaacgaat tcgcccacc 
      ggtacgccg aaaccggca acacggtct acatgtact 
      actacaagc accggacag tggcgaatc cctggccct 
      cctggaccg gcgtgatgc acgccgacg aaatcaact 
      acgtcttcg gggaaccac tcaatccca ctaaaagct 
      acacagcac aagaagtcg atctgagta agcgaatca 
      tgagatact gggccaatt tcgccaaga cgggcaacc 
      cgagtcagt cgccgaacg gcgtttgga cacccactt 
      tctggcccc cgcacacag ctttcggaa gggagtttc 
      tcaccctcg atgtcaact ccactgcca cagggaggg 
      gacctagac tcaagcagt gtgccttct ggaagaaat 
      acctacctc aacttcagc agcaaacga gcgagcttc 
      tgaaccaac cgccccggc aaaactgta ccgacgcgg 
      ccagttcgt tgcgatggt caagagacg gcgctgccg 
      gcctcctga tggtgtcga cagtggcgg cgctcctcg 
      cggggcctt tctaactta gagaaaagt ccgtttagg 
      agttcccct gaagcgcat tagaaacta gcgccttga 
      aacatatct ctagtattg tgtgtgagg tattcaaaa 
      tcaacacga cagaaataa gaaaaatgg gatcggttt 
      atagatttt cgcttttat tgctacgta gagtcaatc 
      aatgctgca gcggactcg cttgtgacg taacaaaat 
      tcatgttca cccggccta tacaggtgg ctgcaatgc 
      gatcggaat tgtcgaatc gctgaaaaa ctaaatcga 
      ggaaaagaa aattttcag ttttttctt tcatatcgg 
      tggttgtgc tgacgagag ggcgctgcc agcgaccat 
      tcgcactaa atttttata ctacttgta tatgatacg 
      gaccagtgt tacgaggtt gcagagatt gcttcttag 
      cagcttaat gttagggcc tataactgt tagtactta 
      gccactatc acttagttc tagggctgt gcatacatg 
      tgaaacgaa ttttcgtgt ctcccggat ctgtcgctc 
      acccagcaa agatggaaa acacaggga cgaacaccc 
      tgacacaaa tgccaacct agtaacaac aaatactct 
      gctacacta tgtctttgc tgccttatt caagaaata 
      gttgacaaa taaactatt ggatctctc tatcctagt 
      ctagtagat agtactcta gtgcctccg cgatagtag 
      tattagcgt tctgagtga tttttatcg atttaagaa 
      acattctca tgtcacaca atcactcag tcgcctttt 
      ttcttatgc tcgccctgc atttactac tcaaacata 
      tccagatta tggtgcaaa acaatacta tctaaaata 
      acctaaata aaaacttag aaaatactc tcaaaaaac 
      actgtacat aattaaaaa actcatgtt tagctgaat 
      tgaatgtta gtaccagga cgcggacgc catgttgcc 
      atcccccac ctcactaaa cctgtgaat accccgatc 
      agtattacc gactctccc gacacacgt gccactaga 
      cattctgac tgaactttc tttcataat acaattatt 
      tttcttatt agaacgaaa catagcgtc cgcagtcac 
      tctgtagat aggtgttag ttaagttat tggcatcga 
      taggtaaga agtggcaat gcttctgaa ttaattaaa 
      gtcaaacta ataattgtt atcaccgaa agcagatgg 
      cgccgcgga aagatggcg aacgagata acgcacgtt 
      cttcgtatt tccccgttt caccttctg tgaaacgac 
      cagtaatat tgtaaattt tgtaacgtt gtcgtagat 
      tttcataaa attgcactg ggtgtggct atagataat 
      aatcgttat taagttttt aggtaaaat tcaaatttg 
      agagaaaac aattggttt cttcaacac gctgtacta 
      gggtcgctt attactatg taacaaatg acaaagtgt 
      taattgcag tttaatgtg agcattcac aacaaaatg 
      tatgttatt ttactattg aaacaaact tttataaga 
      cactgactg gcaaaaaca tttcaaaaa ggaattata 
      taaaacaat gtctcgaaa aataagtat taggtgttc 
      caaaaaata ataataatg aacatatca ttataggct 
      agtcgaata gaataaatt acatatcca tgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY