| General information | |||
| TC014400 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10089233..10105067 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014400 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05625 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC014400 RA
MSILEQSRG LPLIQGMPP SMPPMDGPP NSEVLLALL ARNKALEAT IPKCGGCHE LILDRFILK VADRTWHAK CLQCSDCRI QLTDKCFAR NGQLFCKED FFKRFGTKC AGCDLGIPP TQVVRRAQD NVYHLQCFS CVMCARQLN TGDEFYLME DRKLVCKPD YEAAKSKAA ECLDGDQPN KRPRTTITA KQLETLKNA YNNSPKPAR HVREQLSQD TGLDMRVVQ VWFQNRRAK EKRLKKDAG RTRWSQYFR SMKGGSSPR HDKLLDKDE MKVDLDSFS HHDLSDDSY GTVVNMSMD QDGSSPHSG IGRPSFLHG ATSPSYLPG HTPPHGHEP FPYPDQLAV YASLGQGPP PGMPLPHNM PPGADTDIS NDSSPRGYP DFPPSPDSW LGEPSSAHY
- >TC014400 RB
MISQCNEDR VGLDIEMYR VPPISIDDI PQVLLSTIP KCGGCHELI LDRFILKVA DRTWHAKCL QCSDCRIQL TDKCFARNG QLFCKEDFF KRFGTKCAG CDLGIPPTQ VVRRAQDNV YHLQCFSCV MCARQLNTG DEFYLMEDR KLVCKPDYE AAKSKAAEC LDGDQPNKR PRTTITAKQ LETLKNAYN NSPKPARHV REQLSQDTG LDMRVVQVW FQNRRAKEK RLKKDAGRT RWSQYFRSM KGGSSPRHD KLLDKDEMK VDLDSFSHH DLSDDSYGT VVNMSMDQD GSSPHSGIG RPSFLHGAT SPSYLPGHT PPHGHEPFP YPDQLAVYA SLGQGPPPG MPLPHNMPP GADTDISND SSPRGYPDF PPSPDSWLG EPSSAHY
- >TC014400 RC
MSILEQSRG LPLIQGMPP SMPPMDGPP NSEVLLALL ARNKALEER GGRAAPAVI IAGGTAAGT SSLFNRCSA ATDENSDFG GQMEAPPRP QQMSRTCHL VDFGHATDM WPMSAAKIM FPFLHRREP TQNLNAVLT PRLLGPQCN EDRVGLDIE MYRVPPISI DDIPQVLLS TIPKCGGCH ELILDRFIL KVADRTWHA KCLQCSDCR IQLTDKCFA RNGQLFCKE DFFKRFGTK CAGCDLGIP PTQVVRRAQ DNVYHLQCF SCVMCARQL NTGDEFYLM EDRKLVCKP DYEAAKSKA AECLDGDQP NKRPRTTIT AKQLETLKN AYNNSPKPA RHVREQLSQ DTGLDMRVV QVWFQNRRA KEKRLKKDA GRTRWSQYF RSMKGGSSP RHDKLLDKD EMKVDLDSF SHHDLSDDS YGTVVNMSM DQDGSSPHS GIGRPSFLH GATSPSYLP GHTPPHGHE PFPYPDQLA VYASLGQGP PPGMPLPHN MPPGADTDI SNDSSPRGY PDFPPSPDS WLGEPSSAH Y
- >TC014400 RA
atgtccatt ctggagcag tcgcgaggg ctcccgttg attcaagga atgcccccc agcatgccc cccatggac ggccccccc aactccgag gtgctgctc gcactcctg gcccgcaac aaggcgctc gaagccaca atccccaaa tgtggcggg tgccacgag ctgatcctc gaccgtttc atcctcaaa gtggcggac aggacgtgg cacgcgaaa tgccttcaa tgcagcgac tgtcgcatc caactgacg gacaaatgc ttcgcccgc aacggtcag ctcttctgc aaggaggac tttttcaaa cggttcggc acgaagtgt gctggatgc gacctgggg atccctccg acgcaggtc gtccgaaga gcgcaagac aacgtctac cacctgcag tgcttctcg tgcgtcatg tgcgccaga caactcaac accggagac gaattctac ctcatggag gaccgcaag ttggtctgc aagcccgac tacgaggcc gccaagtcc aaagccgcc gaatgcctc gatggggac cagcccaac aagaggccg aggaccacc atcacggca aaacaactg gaaacgctc aaaaacgcc tacaacaac agtccgaag cccgcaagg catgtcagg gagcaactc agccaggac acaggcctc gacatgcgg gtcgtccaa gtgtggttc cagaataga cgagcgaaa gagaagcgg ctaaaaaag gacgcggga cggactcgt tggagccag tacttccgg tccatgaag ggcggatcg tccccccga cacgacaag ctcctggac aaggacgaa atgaaagtc gacctggac agtttcagc catcacgat ctgagcgac gacagctac ggcaccgtg gtcaacatg agcatggac caggacggg tcgtcgccc cacagcggc atcggtcgg ccctctttc ctccacggc gccacctcc ccgtcctac ctccccggc catacgccg ccccacggc cacgagccg ttcccgtac cccgaccag ttggcggtg tacgccagt ctggggcag gggccgcct cccggcatg cccttgccc cacaatatg cctccaggg gccgacacc gacatcagc aacgacagc agtccgcgg ggatacccc gattttcct ccgagtccc gactcgtgg ctgggggag ccgtccagt gcccactac tga
- >TC014400 RB
atgatcagt cagtgcaac gaggacagg gtcggactc gatatcgag atgtacagg gtgccgccg atttcgatc gacgatatt ccgcaagtg ctcttgtcc acaatcccc aaatgtggc gggtgccac gagctgatc ctcgaccgt ttcatcctc aaagtggcg gacaggacg tggcacgcg aaatgcctt caatgcagc gactgtcgc atccaactg acggacaaa tgcttcgcc cgcaacggt cagctcttc tgcaaggag gactttttc aaacggttc ggcacgaag tgtgctgga tgcgacctg gggatccct ccgacgcag gtcgtccga agagcgcaa gacaacgtc taccacctg cagtgcttc tcgtgcgtc atgtgcgcc agacaactc aacaccgga gacgaattc tacctcatg gaggaccgc aagttggtc tgcaagccc gactacgag gccgccaag tccaaagcc gccgaatgc ctcgatggg gaccagccc aacaagagg ccgaggacc accatcacg gcaaaacaa ctggaaacg ctcaaaaac gcctacaac aacagtccg aagcccgca aggcatgtc agggagcaa ctcagccag gacacaggc ctcgacatg cgggtcgtc caagtgtgg ttccagaat agacgagcg aaagagaag cggctaaaa aaggacgcg ggacggact cgttggagc cagtacttc cggtccatg aagggcgga tcgtccccc cgacacgac aagctcctg gacaaggac gaaatgaaa gtcgacctg gacagtttc agccatcac gatctgagc gacgacagc tacggcacc gtggtcaac atgagcatg gaccaggac gggtcgtcg ccccacagc ggcatcggt cggccctct ttcctccac ggcgccacc tccccgtcc tacctcccc ggccatacg ccgccccac ggccacgag ccgttcccg taccccgac cagttggcg gtgtacgcc agtctgggg caggggccg cctcccggc atgcccttg ccccacaat atgcctcca ggggccgac accgacatc agcaacgac agcagtccg cggggatac cccgatttt cctccgagt cccgactcg tggctgggg gagccgtcc agtgcccac tactga
- >TC014400 RC
atgtccatt ctggagcag tcgcgaggg ctcccgttg attcaagga atgcccccc agcatgccc cccatggac ggccccccc aactccgag gtgctgctc gcactcctg gcccgcaac aaggcgctc gaagagcgg ggagggcgg gccgctccc gccgtaatc atcgccggg gggacggca gcagggacg tcgtcccta ttcaaccgg tgttccgcc gcgacggac gaaaactca gactttggt gggcagatg gaagcccca cctaggccc cagcagatg agtcgcacg tgccacttg gtggacttc ggacacgcg accgacatg tggccgatg agtgctgca aagattatg tttcccttt ttacatcgc agggaaccc acacaaaac ttaaacgcg gtactaaca ccacggtta ttagggccg cagtgcaac gaggacagg gtcggactc gatatcgag atgtacagg gtgccgccg atttcgatc gacgatatt ccgcaagtg ctcttgtcc acaatcccc aaatgtggc gggtgccac gagctgatc ctcgaccgt ttcatcctc aaagtggcg gacaggacg tggcacgcg aaatgcctt caatgcagc gactgtcgc atccaactg acggacaaa tgcttcgcc cgcaacggt cagctcttc tgcaaggag gactttttc aaacggttc ggcacgaag tgtgctgga tgcgacctg gggatccct ccgacgcag gtcgtccga agagcgcaa gacaacgtc taccacctg cagtgcttc tcgtgcgtc atgtgcgcc agacaactc aacaccgga gacgaattc tacctcatg gaggaccgc aagttggtc tgcaagccc gactacgag gccgccaag tccaaagcc gccgaatgc ctcgatggg gaccagccc aacaagagg ccgaggacc accatcacg gcaaaacaa ctggaaacg ctcaaaaac gcctacaac aacagtccg aagcccgca aggcatgtc agggagcaa ctcagccag gacacaggc ctcgacatg cgggtcgtc caagtgtgg ttccagaat agacgagcg aaagagaag cggctaaaa aaggacgcg ggacggact cgttggagc cagtacttc cggtccatg aagggcgga tcgtccccc cgacacgac aagctcctg gacaaggac gaaatgaaa gtcgacctg gacagtttc agccatcac gatctgagc gacgacagc tacggcacc gtggtcaac atgagcatg gaccaggac gggtcgtcg ccccacagc ggcatcggt cggccctct ttcctccac ggcgccacc tccccgtcc tacctcccc ggccatacg ccgccccac ggccacgag ccgttcccg taccccgac cagttggcg gtgtacgcc agtctgggg caggggccg cctcccggc atgcccttg ccccacaat atgcctcca ggggccgac accgacatc agcaacgac agcagtccg cggggatac cccgatttt cctccgagt cccgactcg tggctgggg gagccgtcc agtgcccac tactga
- >TC014400 RA
tcggggtga aacacttag tgaaattaa acggagccg actaagtta acgcaaatt gaaaaatat aacctacat gagccatac gtccggaaa tgatcgccg cagtgtaaa aaatgccgg aaaaaatca ccgccatcg cccggatcg gagattaaa cccctcggt gcgcgaaaa agtgctgtg actatgtcc attctggag cagtcgcga gggctcccg ttgattcaa ggaatgccc cccagcatg ccccccatg gacggcccc cccaactcc gaggtgctg ctcgcactc ctggcccgc aacaaggcg ctcgaagcc acaatcccc aaatgtggc gggtgccac gagctgatc ctcgaccgt ttcatcctc aaagtggcg gacaggacg tggcacgcg aaatgcctt caatgcagc gactgtcgc atccaactg acggacaaa tgcttcgcc cgcaacggt cagctcttc tgcaaggag gactttttc aaacggttc ggcacgaag tgtgctgga tgcgacctg gggatccct ccgacgcag gtcgtccga agagcgcaa gacaacgtc taccacctg cagtgcttc tcgtgcgtc atgtgcgcc agacaactc aacaccgga gacgaattc tacctcatg gaggaccgc aagttggtc tgcaagccc gactacgag gccgccaag tccaaagcc gccgaatgc ctcgatggg gaccagccc aacaagagg ccgaggacc accatcacg gcaaaacaa ctggaaacg ctcaaaaac gcctacaac aacagtccg aagcccgca aggcatgtc agggagcaa ctcagccag gacacaggc ctcgacatg cgggtcgtc caagtgtgg ttccagaat agacgagcg aaagagaag cggctaaaa aaggacgcg ggacggact cgttggagc cagtacttc cggtccatg aagggcgga tcgtccccc cgacacgac aagctcctg gacaaggac gaaatgaaa gtcgacctg gacagtttc agccatcac gatctgagc gacgacagc tacggcacc gtggtcaac atgagcatg gaccaggac gggtcgtcg ccccacagc ggcatcggt cggccctct ttcctccac ggcgccacc tccccgtcc tacctcccc ggccatacg ccgccccac ggccacgag ccgttcccg taccccgac cagttggcg gtgtacgcc agtctgggg caggggccg cctcccggc atgcccttg ccccacaat atgcctcca ggggccgac accgacatc agcaacgac agcagtccg cggggatac cccgatttt cctccgagt cccgactcg tggctgggg gagccgtcc agtgcccac tactgaccc agcaaatca caaaaaacg aacactgtc acgatattt atatgcgat ttaaagttt ctattcatg tgtcattat gagtgtata cagagtggc acggagcac aacaatgac gggccgaag tgtacagct cttgttcta gtcgagatt acagagata ttgtacaat aagtgtcag gtttaacat tctagt
- >TC014400 RB
gggcaataa agggaagaa aaatatccg agtttgggg gcgttctgc gggaggtgg agcgtcgcg acgctgccg tcgcgtgtc cgccaatgg cagcgccga taggaaaaa caagggatc gtctcttta ataaagtac ggtttacac aaatctagg acgtgtcat cgatgggtg ggcggttga agggtcgat aaaagagag ctcgcgacg gcgcatttt cgaattttc gaggcagtc ggtcggcgg tcgttgacg ggtcaattg ggaccattg aatttttcg agacgcgaa tttattcga aatgatcag tcagtgcaa cgaggacag ggtcggact cgatatcga gatgtacag ggtgccgcc gatttcgat cgacgatat tccgcaagt gctcttgtc cacaatccc caaatgtgg cgggtgcca cgagctgat cctcgaccg tttcatcct caaagtggc ggacaggac gtggcacgc gaaatgcct tcaatgcag cgactgtcg catccaact gacggacaa atgcttcgc ccgcaacgg tcagctctt ctgcaagga ggacttttt caaacggtt cggcacgaa gtgtgctgg atgcgacct ggggatccc tccgacgca ggtcgtccg aagagcgca agacaacgt ctaccacct gcagtgctt ctcgtgcgt catgtgcgc cagacaact caacaccgg agacgaatt ctacctcat ggaggaccg caagttggt ctgcaagcc cgactacga ggccgccaa gtccaaagc cgccgaatg cctcgatgg ggaccagcc caacaagag gccgaggac caccatcac ggcaaaaca actggaaac gctcaaaaa cgcctacaa caacagtcc gaagcccgc aaggcatgt cagggagca actcagcca ggacacagg cctcgacat gcgggtcgt ccaagtgtg gttccagaa tagacgagc gaaagagaa gcggctaaa aaaggacgc gggacggac tcgttggag ccagtactt ccggtccat gaagggcgg atcgtcccc ccgacacga caagctcct ggacaagga cgaaatgaa agtcgacct ggacagttt cagccatca cgatctgag cgacgacag ctacggcac cgtggtcaa catgagcat ggaccagga cgggtcgtc gccccacag cggcatcgg tcggccctc tttcctcca cggcgccac ctccccgtc ctacctccc cggccatac gccgcccca cggccacga gccgttccc gtaccccga ccagttggc ggtgtacgc cagtctggg gcaggggcc gcctcccgg catgccctt gccccacaa tatgcctcc aggggccga caccgacat cagcaacga cagcagtcc gcggggata ccccgattt tcctccgag tcccgactc gtggctggg ggagccgtc cagtgccca ctactgacc cagcaaatc acaaaaaac gaacactgt cacgatatt tatatgcga tttaaagtt tctattcat gtgtcatta tgagtgtat acagagtgg cacggagca caacaatga cgggccgaa gtgtacagc tcttgttct agtcgagat tacagagat attgtacaa taagtgtca ggtttaaca ttctagt
- >TC014400 RC
tcggggtga aacacttag tgaaattaa acggagccg actaagtta acgcaaatt gaaaaatat aacctacat gagccatac gtccggaaa tgatcgccg cagtgtaaa aaatgccgg aaaaaatca ccgccatcg cccggatcg gagattaaa cccctcggt gcgcgaaaa agtgctgtg actatgtcc attctggag cagtcgcga gggctcccg ttgattcaa ggaatgccc cccagcatg ccccccatg gacggcccc cccaactcc gaggtgctg ctcgcactc ctggcccgc aacaaggcg ctcgaagag cggggaggg cgggccgct cccgccgta atcatcgcc ggggggacg gcagcaggg acgtcgtcc ctattcaac cggtgttcc gccgcgacg gacgaaaac tcagacttt ggtgggcag atggaagcc ccacctagg ccccagcag atgagtcgc acgtgccac ttggtggac ttcggacac gcgaccgac atgtggccg atgagtgct gcaaagatt atgtttccc tttttacat cgcagggaa cccacacaa aacttaaac gcggtacta acaccacgg ttattaggg ccgcagtgc aacgaggac agggtcgga ctcgatatc gagatgtac agggtgccg ccgatttcg atcgacgat attccgcaa gtgctcttg tccacaatc cccaaatgt ggcgggtgc cacgagctg atcctcgac cgtttcatc ctcaaagtg gcggacagg acgtggcac gcgaaatgc cttcaatgc agcgactgt cgcatccaa ctgacggac aaatgcttc gcccgcaac ggtcagctc ttctgcaag gaggacttt ttcaaacgg ttcggcacg aagtgtgct ggatgcgac ctggggatc cctccgacg caggtcgtc cgaagagcg caagacaac gtctaccac ctgcagtgc ttctcgtgc gtcatgtgc gccagacaa ctcaacacc ggagacgaa ttctacctc atggaggac cgcaagttg gtctgcaag cccgactac gaggccgcc aagtccaaa gccgccgaa tgcctcgat ggggaccag cccaacaag aggccgagg accaccatc acggcaaaa caactggaa acgctcaaa aacgcctac aacaacagt ccgaagccc gcaaggcat gtcagggag caactcagc caggacaca ggcctcgac atgcgggtc gtccaagtg tggttccag aatagacga gcgaaagag aagcggcta aaaaaggac gcgggacgg actcgttgg agccagtac ttccggtcc atgaagggc ggatcgtcc ccccgacac gacaagctc ctggacaag gacgaaatg aaagtcgac ctggacagt ttcagccat cacgatctg agcgacgac agctacggc accgtggtc aacatgagc atggaccag gacgggtcg tcgccccac agcggcatc ggtcggccc tctttcctc cacggcgcc acctccccg tcctacctc cccggccat acgccgccc cacggccac gagccgttc ccgtacccc gaccagttg gcggtgtac gccagtctg gggcagggg ccgcctccc ggcatgccc ttgccccac aatatgcct ccaggggcc gacaccgac atcagcaac gacagcagt ccgcgggga taccccgat tttcctccg agtcccgac tcgtggctg ggggagccg tccagtgcc cactactga cccagcaaa tcacaaaaa acgaacact gtcacgata tttatatgc gatttaaag tttctattc atgtgtcat tatgagtgt atacagagt ggcacggag cacaacaat gacgggccg aagtgtaca gctcttgtt ctagtcgag attacagag atattgtac aataagtgt caggtttaa cattctagt
