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Gene information
General information
TC014400 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10089233..10105067
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014400
    iBeetle screen iB_05625

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014400 RA
      MSILEQSRG LPLIQGMPP SMPPMDGPP NSEVLLALL 
      ARNKALEAT IPKCGGCHE LILDRFILK VADRTWHAK 
      CLQCSDCRI QLTDKCFAR NGQLFCKED FFKRFGTKC 
      AGCDLGIPP TQVVRRAQD NVYHLQCFS CVMCARQLN 
      TGDEFYLME DRKLVCKPD YEAAKSKAA ECLDGDQPN 
      KRPRTTITA KQLETLKNA YNNSPKPAR HVREQLSQD 
      TGLDMRVVQ VWFQNRRAK EKRLKKDAG RTRWSQYFR 
      SMKGGSSPR HDKLLDKDE MKVDLDSFS HHDLSDDSY 
      GTVVNMSMD QDGSSPHSG IGRPSFLHG ATSPSYLPG 
      HTPPHGHEP FPYPDQLAV YASLGQGPP PGMPLPHNM 
      PPGADTDIS NDSSPRGYP DFPPSPDSW LGEPSSAHY
    • >TC014400 RB
      MISQCNEDR VGLDIEMYR VPPISIDDI PQVLLSTIP 
      KCGGCHELI LDRFILKVA DRTWHAKCL QCSDCRIQL 
      TDKCFARNG QLFCKEDFF KRFGTKCAG CDLGIPPTQ 
      VVRRAQDNV YHLQCFSCV MCARQLNTG DEFYLMEDR 
      KLVCKPDYE AAKSKAAEC LDGDQPNKR PRTTITAKQ 
      LETLKNAYN NSPKPARHV REQLSQDTG LDMRVVQVW 
      FQNRRAKEK RLKKDAGRT RWSQYFRSM KGGSSPRHD 
      KLLDKDEMK VDLDSFSHH DLSDDSYGT VVNMSMDQD 
      GSSPHSGIG RPSFLHGAT SPSYLPGHT PPHGHEPFP 
      YPDQLAVYA SLGQGPPPG MPLPHNMPP GADTDISND 
      SSPRGYPDF PPSPDSWLG EPSSAHY
    • >TC014400 RC
      MSILEQSRG LPLIQGMPP SMPPMDGPP NSEVLLALL 
      ARNKALEER GGRAAPAVI IAGGTAAGT SSLFNRCSA 
      ATDENSDFG GQMEAPPRP QQMSRTCHL VDFGHATDM 
      WPMSAAKIM FPFLHRREP TQNLNAVLT PRLLGPQCN 
      EDRVGLDIE MYRVPPISI DDIPQVLLS TIPKCGGCH 
      ELILDRFIL KVADRTWHA KCLQCSDCR IQLTDKCFA 
      RNGQLFCKE DFFKRFGTK CAGCDLGIP PTQVVRRAQ 
      DNVYHLQCF SCVMCARQL NTGDEFYLM EDRKLVCKP 
      DYEAAKSKA AECLDGDQP NKRPRTTIT AKQLETLKN 
      AYNNSPKPA RHVREQLSQ DTGLDMRVV QVWFQNRRA 
      KEKRLKKDA GRTRWSQYF RSMKGGSSP RHDKLLDKD 
      EMKVDLDSF SHHDLSDDS YGTVVNMSM DQDGSSPHS 
      GIGRPSFLH GATSPSYLP GHTPPHGHE PFPYPDQLA 
      VYASLGQGP PPGMPLPHN MPPGADTDI SNDSSPRGY 
      PDFPPSPDS WLGEPSSAH Y
    CDS sequence
    • >TC014400 RA
      atgtccatt ctggagcag tcgcgaggg ctcccgttg 
      attcaagga atgcccccc agcatgccc cccatggac 
      ggccccccc aactccgag gtgctgctc gcactcctg 
      gcccgcaac aaggcgctc gaagccaca atccccaaa 
      tgtggcggg tgccacgag ctgatcctc gaccgtttc 
      atcctcaaa gtggcggac aggacgtgg cacgcgaaa 
      tgccttcaa tgcagcgac tgtcgcatc caactgacg 
      gacaaatgc ttcgcccgc aacggtcag ctcttctgc 
      aaggaggac tttttcaaa cggttcggc acgaagtgt 
      gctggatgc gacctgggg atccctccg acgcaggtc 
      gtccgaaga gcgcaagac aacgtctac cacctgcag 
      tgcttctcg tgcgtcatg tgcgccaga caactcaac 
      accggagac gaattctac ctcatggag gaccgcaag 
      ttggtctgc aagcccgac tacgaggcc gccaagtcc 
      aaagccgcc gaatgcctc gatggggac cagcccaac 
      aagaggccg aggaccacc atcacggca aaacaactg 
      gaaacgctc aaaaacgcc tacaacaac agtccgaag 
      cccgcaagg catgtcagg gagcaactc agccaggac 
      acaggcctc gacatgcgg gtcgtccaa gtgtggttc 
      cagaataga cgagcgaaa gagaagcgg ctaaaaaag 
      gacgcggga cggactcgt tggagccag tacttccgg 
      tccatgaag ggcggatcg tccccccga cacgacaag 
      ctcctggac aaggacgaa atgaaagtc gacctggac 
      agtttcagc catcacgat ctgagcgac gacagctac 
      ggcaccgtg gtcaacatg agcatggac caggacggg 
      tcgtcgccc cacagcggc atcggtcgg ccctctttc 
      ctccacggc gccacctcc ccgtcctac ctccccggc 
      catacgccg ccccacggc cacgagccg ttcccgtac 
      cccgaccag ttggcggtg tacgccagt ctggggcag 
      gggccgcct cccggcatg cccttgccc cacaatatg 
      cctccaggg gccgacacc gacatcagc aacgacagc 
      agtccgcgg ggatacccc gattttcct ccgagtccc 
      gactcgtgg ctgggggag ccgtccagt gcccactac 
      tga
    • >TC014400 RB
      atgatcagt cagtgcaac gaggacagg gtcggactc 
      gatatcgag atgtacagg gtgccgccg atttcgatc 
      gacgatatt ccgcaagtg ctcttgtcc acaatcccc 
      aaatgtggc gggtgccac gagctgatc ctcgaccgt 
      ttcatcctc aaagtggcg gacaggacg tggcacgcg 
      aaatgcctt caatgcagc gactgtcgc atccaactg 
      acggacaaa tgcttcgcc cgcaacggt cagctcttc 
      tgcaaggag gactttttc aaacggttc ggcacgaag 
      tgtgctgga tgcgacctg gggatccct ccgacgcag 
      gtcgtccga agagcgcaa gacaacgtc taccacctg 
      cagtgcttc tcgtgcgtc atgtgcgcc agacaactc 
      aacaccgga gacgaattc tacctcatg gaggaccgc 
      aagttggtc tgcaagccc gactacgag gccgccaag 
      tccaaagcc gccgaatgc ctcgatggg gaccagccc 
      aacaagagg ccgaggacc accatcacg gcaaaacaa 
      ctggaaacg ctcaaaaac gcctacaac aacagtccg 
      aagcccgca aggcatgtc agggagcaa ctcagccag 
      gacacaggc ctcgacatg cgggtcgtc caagtgtgg 
      ttccagaat agacgagcg aaagagaag cggctaaaa 
      aaggacgcg ggacggact cgttggagc cagtacttc 
      cggtccatg aagggcgga tcgtccccc cgacacgac 
      aagctcctg gacaaggac gaaatgaaa gtcgacctg 
      gacagtttc agccatcac gatctgagc gacgacagc 
      tacggcacc gtggtcaac atgagcatg gaccaggac 
      gggtcgtcg ccccacagc ggcatcggt cggccctct 
      ttcctccac ggcgccacc tccccgtcc tacctcccc 
      ggccatacg ccgccccac ggccacgag ccgttcccg 
      taccccgac cagttggcg gtgtacgcc agtctgggg 
      caggggccg cctcccggc atgcccttg ccccacaat 
      atgcctcca ggggccgac accgacatc agcaacgac 
      agcagtccg cggggatac cccgatttt cctccgagt 
      cccgactcg tggctgggg gagccgtcc agtgcccac 
      tactga
    • >TC014400 RC
      atgtccatt ctggagcag tcgcgaggg ctcccgttg 
      attcaagga atgcccccc agcatgccc cccatggac 
      ggccccccc aactccgag gtgctgctc gcactcctg 
      gcccgcaac aaggcgctc gaagagcgg ggagggcgg 
      gccgctccc gccgtaatc atcgccggg gggacggca 
      gcagggacg tcgtcccta ttcaaccgg tgttccgcc 
      gcgacggac gaaaactca gactttggt gggcagatg 
      gaagcccca cctaggccc cagcagatg agtcgcacg 
      tgccacttg gtggacttc ggacacgcg accgacatg 
      tggccgatg agtgctgca aagattatg tttcccttt 
      ttacatcgc agggaaccc acacaaaac ttaaacgcg 
      gtactaaca ccacggtta ttagggccg cagtgcaac 
      gaggacagg gtcggactc gatatcgag atgtacagg 
      gtgccgccg atttcgatc gacgatatt ccgcaagtg 
      ctcttgtcc acaatcccc aaatgtggc gggtgccac 
      gagctgatc ctcgaccgt ttcatcctc aaagtggcg 
      gacaggacg tggcacgcg aaatgcctt caatgcagc 
      gactgtcgc atccaactg acggacaaa tgcttcgcc 
      cgcaacggt cagctcttc tgcaaggag gactttttc 
      aaacggttc ggcacgaag tgtgctgga tgcgacctg 
      gggatccct ccgacgcag gtcgtccga agagcgcaa 
      gacaacgtc taccacctg cagtgcttc tcgtgcgtc 
      atgtgcgcc agacaactc aacaccgga gacgaattc 
      tacctcatg gaggaccgc aagttggtc tgcaagccc 
      gactacgag gccgccaag tccaaagcc gccgaatgc 
      ctcgatggg gaccagccc aacaagagg ccgaggacc 
      accatcacg gcaaaacaa ctggaaacg ctcaaaaac 
      gcctacaac aacagtccg aagcccgca aggcatgtc 
      agggagcaa ctcagccag gacacaggc ctcgacatg 
      cgggtcgtc caagtgtgg ttccagaat agacgagcg 
      aaagagaag cggctaaaa aaggacgcg ggacggact 
      cgttggagc cagtacttc cggtccatg aagggcgga 
      tcgtccccc cgacacgac aagctcctg gacaaggac 
      gaaatgaaa gtcgacctg gacagtttc agccatcac 
      gatctgagc gacgacagc tacggcacc gtggtcaac 
      atgagcatg gaccaggac gggtcgtcg ccccacagc 
      ggcatcggt cggccctct ttcctccac ggcgccacc 
      tccccgtcc tacctcccc ggccatacg ccgccccac 
      ggccacgag ccgttcccg taccccgac cagttggcg 
      gtgtacgcc agtctgggg caggggccg cctcccggc 
      atgcccttg ccccacaat atgcctcca ggggccgac 
      accgacatc agcaacgac agcagtccg cggggatac 
      cccgatttt cctccgagt cccgactcg tggctgggg 
      gagccgtcc agtgcccac tactga
    mRNA sequence
    • >TC014400 RA
      tcggggtga aacacttag tgaaattaa acggagccg 
      actaagtta acgcaaatt gaaaaatat aacctacat 
      gagccatac gtccggaaa tgatcgccg cagtgtaaa 
      aaatgccgg aaaaaatca ccgccatcg cccggatcg 
      gagattaaa cccctcggt gcgcgaaaa agtgctgtg 
      actatgtcc attctggag cagtcgcga gggctcccg 
      ttgattcaa ggaatgccc cccagcatg ccccccatg 
      gacggcccc cccaactcc gaggtgctg ctcgcactc 
      ctggcccgc aacaaggcg ctcgaagcc acaatcccc 
      aaatgtggc gggtgccac gagctgatc ctcgaccgt 
      ttcatcctc aaagtggcg gacaggacg tggcacgcg 
      aaatgcctt caatgcagc gactgtcgc atccaactg 
      acggacaaa tgcttcgcc cgcaacggt cagctcttc 
      tgcaaggag gactttttc aaacggttc ggcacgaag 
      tgtgctgga tgcgacctg gggatccct ccgacgcag 
      gtcgtccga agagcgcaa gacaacgtc taccacctg 
      cagtgcttc tcgtgcgtc atgtgcgcc agacaactc 
      aacaccgga gacgaattc tacctcatg gaggaccgc 
      aagttggtc tgcaagccc gactacgag gccgccaag 
      tccaaagcc gccgaatgc ctcgatggg gaccagccc 
      aacaagagg ccgaggacc accatcacg gcaaaacaa 
      ctggaaacg ctcaaaaac gcctacaac aacagtccg 
      aagcccgca aggcatgtc agggagcaa ctcagccag 
      gacacaggc ctcgacatg cgggtcgtc caagtgtgg 
      ttccagaat agacgagcg aaagagaag cggctaaaa 
      aaggacgcg ggacggact cgttggagc cagtacttc 
      cggtccatg aagggcgga tcgtccccc cgacacgac 
      aagctcctg gacaaggac gaaatgaaa gtcgacctg 
      gacagtttc agccatcac gatctgagc gacgacagc 
      tacggcacc gtggtcaac atgagcatg gaccaggac 
      gggtcgtcg ccccacagc ggcatcggt cggccctct 
      ttcctccac ggcgccacc tccccgtcc tacctcccc 
      ggccatacg ccgccccac ggccacgag ccgttcccg 
      taccccgac cagttggcg gtgtacgcc agtctgggg 
      caggggccg cctcccggc atgcccttg ccccacaat 
      atgcctcca ggggccgac accgacatc agcaacgac 
      agcagtccg cggggatac cccgatttt cctccgagt 
      cccgactcg tggctgggg gagccgtcc agtgcccac 
      tactgaccc agcaaatca caaaaaacg aacactgtc 
      acgatattt atatgcgat ttaaagttt ctattcatg 
      tgtcattat gagtgtata cagagtggc acggagcac 
      aacaatgac gggccgaag tgtacagct cttgttcta 
      gtcgagatt acagagata ttgtacaat aagtgtcag 
      gtttaacat tctagt
    • >TC014400 RB
      gggcaataa agggaagaa aaatatccg agtttgggg 
      gcgttctgc gggaggtgg agcgtcgcg acgctgccg 
      tcgcgtgtc cgccaatgg cagcgccga taggaaaaa 
      caagggatc gtctcttta ataaagtac ggtttacac 
      aaatctagg acgtgtcat cgatgggtg ggcggttga 
      agggtcgat aaaagagag ctcgcgacg gcgcatttt 
      cgaattttc gaggcagtc ggtcggcgg tcgttgacg 
      ggtcaattg ggaccattg aatttttcg agacgcgaa 
      tttattcga aatgatcag tcagtgcaa cgaggacag 
      ggtcggact cgatatcga gatgtacag ggtgccgcc 
      gatttcgat cgacgatat tccgcaagt gctcttgtc 
      cacaatccc caaatgtgg cgggtgcca cgagctgat 
      cctcgaccg tttcatcct caaagtggc ggacaggac 
      gtggcacgc gaaatgcct tcaatgcag cgactgtcg 
      catccaact gacggacaa atgcttcgc ccgcaacgg 
      tcagctctt ctgcaagga ggacttttt caaacggtt 
      cggcacgaa gtgtgctgg atgcgacct ggggatccc 
      tccgacgca ggtcgtccg aagagcgca agacaacgt 
      ctaccacct gcagtgctt ctcgtgcgt catgtgcgc 
      cagacaact caacaccgg agacgaatt ctacctcat 
      ggaggaccg caagttggt ctgcaagcc cgactacga 
      ggccgccaa gtccaaagc cgccgaatg cctcgatgg 
      ggaccagcc caacaagag gccgaggac caccatcac 
      ggcaaaaca actggaaac gctcaaaaa cgcctacaa 
      caacagtcc gaagcccgc aaggcatgt cagggagca 
      actcagcca ggacacagg cctcgacat gcgggtcgt 
      ccaagtgtg gttccagaa tagacgagc gaaagagaa 
      gcggctaaa aaaggacgc gggacggac tcgttggag 
      ccagtactt ccggtccat gaagggcgg atcgtcccc 
      ccgacacga caagctcct ggacaagga cgaaatgaa 
      agtcgacct ggacagttt cagccatca cgatctgag 
      cgacgacag ctacggcac cgtggtcaa catgagcat 
      ggaccagga cgggtcgtc gccccacag cggcatcgg 
      tcggccctc tttcctcca cggcgccac ctccccgtc 
      ctacctccc cggccatac gccgcccca cggccacga 
      gccgttccc gtaccccga ccagttggc ggtgtacgc 
      cagtctggg gcaggggcc gcctcccgg catgccctt 
      gccccacaa tatgcctcc aggggccga caccgacat 
      cagcaacga cagcagtcc gcggggata ccccgattt 
      tcctccgag tcccgactc gtggctggg ggagccgtc 
      cagtgccca ctactgacc cagcaaatc acaaaaaac 
      gaacactgt cacgatatt tatatgcga tttaaagtt 
      tctattcat gtgtcatta tgagtgtat acagagtgg 
      cacggagca caacaatga cgggccgaa gtgtacagc 
      tcttgttct agtcgagat tacagagat attgtacaa 
      taagtgtca ggtttaaca ttctagt
    • >TC014400 RC
      tcggggtga aacacttag tgaaattaa acggagccg 
      actaagtta acgcaaatt gaaaaatat aacctacat 
      gagccatac gtccggaaa tgatcgccg cagtgtaaa 
      aaatgccgg aaaaaatca ccgccatcg cccggatcg 
      gagattaaa cccctcggt gcgcgaaaa agtgctgtg 
      actatgtcc attctggag cagtcgcga gggctcccg 
      ttgattcaa ggaatgccc cccagcatg ccccccatg 
      gacggcccc cccaactcc gaggtgctg ctcgcactc 
      ctggcccgc aacaaggcg ctcgaagag cggggaggg 
      cgggccgct cccgccgta atcatcgcc ggggggacg 
      gcagcaggg acgtcgtcc ctattcaac cggtgttcc 
      gccgcgacg gacgaaaac tcagacttt ggtgggcag 
      atggaagcc ccacctagg ccccagcag atgagtcgc 
      acgtgccac ttggtggac ttcggacac gcgaccgac 
      atgtggccg atgagtgct gcaaagatt atgtttccc 
      tttttacat cgcagggaa cccacacaa aacttaaac 
      gcggtacta acaccacgg ttattaggg ccgcagtgc 
      aacgaggac agggtcgga ctcgatatc gagatgtac 
      agggtgccg ccgatttcg atcgacgat attccgcaa 
      gtgctcttg tccacaatc cccaaatgt ggcgggtgc 
      cacgagctg atcctcgac cgtttcatc ctcaaagtg 
      gcggacagg acgtggcac gcgaaatgc cttcaatgc 
      agcgactgt cgcatccaa ctgacggac aaatgcttc 
      gcccgcaac ggtcagctc ttctgcaag gaggacttt 
      ttcaaacgg ttcggcacg aagtgtgct ggatgcgac 
      ctggggatc cctccgacg caggtcgtc cgaagagcg 
      caagacaac gtctaccac ctgcagtgc ttctcgtgc 
      gtcatgtgc gccagacaa ctcaacacc ggagacgaa 
      ttctacctc atggaggac cgcaagttg gtctgcaag 
      cccgactac gaggccgcc aagtccaaa gccgccgaa 
      tgcctcgat ggggaccag cccaacaag aggccgagg 
      accaccatc acggcaaaa caactggaa acgctcaaa 
      aacgcctac aacaacagt ccgaagccc gcaaggcat 
      gtcagggag caactcagc caggacaca ggcctcgac 
      atgcgggtc gtccaagtg tggttccag aatagacga 
      gcgaaagag aagcggcta aaaaaggac gcgggacgg 
      actcgttgg agccagtac ttccggtcc atgaagggc 
      ggatcgtcc ccccgacac gacaagctc ctggacaag 
      gacgaaatg aaagtcgac ctggacagt ttcagccat 
      cacgatctg agcgacgac agctacggc accgtggtc 
      aacatgagc atggaccag gacgggtcg tcgccccac 
      agcggcatc ggtcggccc tctttcctc cacggcgcc 
      acctccccg tcctacctc cccggccat acgccgccc 
      cacggccac gagccgttc ccgtacccc gaccagttg 
      gcggtgtac gccagtctg gggcagggg ccgcctccc 
      ggcatgccc ttgccccac aatatgcct ccaggggcc 
      gacaccgac atcagcaac gacagcagt ccgcgggga 
      taccccgat tttcctccg agtcccgac tcgtggctg 
      ggggagccg tccagtgcc cactactga cccagcaaa 
      tcacaaaaa acgaacact gtcacgata tttatatgc 
      gatttaaag tttctattc atgtgtcat tatgagtgt 
      atacagagt ggcacggag cacaacaat gacgggccg 
      aagtgtaca gctcttgtt ctagtcgag attacagag 
      atattgtac aataagtgt caggtttaa cattctagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY