Status
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Gene information
General information
TC014398 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10061193..10067180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014398
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05624

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48
    Pupal morphology
    remarks: later than schedule, hours 48
    Fertility
    mature egg not present - Number of animals showing the phenotype: 2
    tropharium structure irregular - Number of animals showing the phenotype: 3

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
    mature egg potentially degenerated - Number of animals showing the phenotype: 1

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014398 RA
      MPKEKQLNR TQSRRAASK EALKQMHLL LGADNNKYE 
      ISSDDDVIE VEVQPPKKK YKLSHRKSS DGNDRDVIV 
      LKPVDESVF DLEVVVEDL APSQKSRLE LLQKETNEV 
      ETTIQQLLT APNTEVVYN GVYTTAVEI TEQIQTILS 
      TPTPAAAST AQFTITNQT PLAAPTPVQ QPVASQSLV 
      LQQQPPSAN NAIPQSYQL VMDPRLGVI VGAVSTSNA 
      SPNTPPLLY QPKVAIPAP GAAAATESP AGKGRRGPI 
      LRQVPANAI PPLAKVSQK TPVAANKTQ NKIQVKPML 
      GKAVQPKQN VVKTIPASP KPIPIKGRP PTTSTSTNF 
      RPTESNVEG GLSMGAKPS KSIDLTGED GRALPDSRE 
      VSFNKLQGR TYPSLVVVA RPHLRVKDL AADRSKLDV 
      KVKSVLMYP PTKFTEWLI QQGLVRSDQ KCSVHTTNP 
      LKLGMYSDA SKFPYSGGY VWISECCPQ RFVSVFSGS 
      IFEGSQHPP MSILKLLYH WCCQTIVQN VTQWVKVDN 
      LYVKGMYTW LRSVCTVAI YSHIKQLGG PGVKVQIGV 
      ISLGTTSQD GNKREVKVE VLGILETTT RHIRLRAVE 
      PLSELERNY KKRFSKILE PVFNWVHPQ SIIVTDLTV 
      DKNTLHQLG FLHVQQNTS NDYANSNRT IMEYLRRIV 
      PRMFQNTLS LLSRPIIQQ FLDELVWRE WYGTTVLQC 
      FDNMVKHIS EQTRFDSGS SLINRLNKV SANPFKNWS 
      ITTYTTSTP SKPVEPAPK RYSRLRKPQ PSAPAPPPV 
      QEVVRPSKS HSPDVPEQM VPLENYYYG TISPTTPSK 
      NNTMINIKC PFCKLTFNT NISLMNHLF KHAHNVSND 
      VQLCKYCLT IVPSANDLL KHINTAHPD ETKFDNGFV 
      CLICESHFR NPFILGKHL SREHNPSEL PYLCGTCGF 
      RCSNHKQAV DHFYQQHDR GTTIQCPFC LKSTTIYSG 
      GRNISQNMN YFIQHLQKH QKKQLAKRC GKCTLWFIQ 
      KEFLKDHQI KMHASQRGK PGLVPAFAP RNGVMVPKS 
      KMDKWPDDA KVINFSTLY FNVNKNLNC KECGQSLSS 
      PKHFASFDS CQNPNCQFS TCCTTAMQN HVAKCKFLN 
      SKLPAEKLP YEMFCICGY SDTDGNMLA KHLAICERK 
      SAYPSQEAA KEATVTHSM LDVLGLVRK PEEATSSKD 
      DLPRLIPTR SEPIILKRG RPRKHPVPP KEVIEVKDD 
      EEPLSISEV RGGVKIEGE KHGMTEIEE IKESENKDV 
      SEKQKEVQE DKSQVGQED HQEKGTEEE TSEVVDKEQ 
      EDHPEKEIE EEASEVGGK GQEEDQGKE IEEETSEVV 
      DKGQEDQQE KEIAEETSE VVDKEQEDQ QEKNIEKET 
      SEIVKAPED QEEKNVEEE TSEVIDKVQ EDPHEEEEK 
      TNDIVDKGQ EDHRNEEVE ETTSGIKEQ EDVQQKEAE 
      DDSGIVDNK SENDEVKCV EEEEIKEHS AEARDNESV 
      SENTKDVNE LQSETVESE SMEVDSCNA SETVETVAI 
      GNQMEKNSE TIDDICPMD TD
    CDS sequence
    • >TC014398 RA
      atgccgaaa gagaaacag ctgaatcgg actcagtca 
      aggcgggcc gctagtaag gaggccctg aaacaaatg 
      catctgttg ctgggagct gataataat aaatatgaa 
      atatcgtca gatgacgat gttatagaa gtcgaggtg 
      cagccacca aagaagaaa tataagtta agtcaccgg 
      aagtcatca gatggaaat gatcgtgac gtcatagtt 
      ttaaaaccc gttgacgaa agtgttttc gacttggaa 
      gtggtcgtc gaagactta gccccaagt cagaagtct 
      cgtctcgag ttgcttcaa aaagaaaca aacgaagtt 
      gaaacaaca attcaacaa ctcctcacc gcccccaac 
      accgaagtc gtctacaat ggggtctac acgaccgct 
      gtcgaaatc actgaacag atacagaca atccttagc 
      acgcccacc cccgctgcc gcctccacc gcccagttc 
      acaatcacg aaccaaact ccgctcgcc gcccccacc 
      cccgtacaa cagccggtc gctagtcag agtctagtt 
      ttgcagcag cagccaccg tctgccaac aatgcgatt 
      ccacaaagc taccaactg gtcatggat ccgcggctt 
      ggtgtcatc gtgggggcg gtgtccacg tcaaacgct 
      agtcccaac acgccgccc cttttgtat caacccaaa 
      gttgcaatt ccggcgccg ggggcggca gcagctaca 
      gaatcgccg gcgggcaag ggacggcgc ggccccatt 
      ttgagacaa gtgcccgct aatgctatc ccaccgctg 
      gcgaaagtg tcgcagaag acgcccgtg gcggccaac 
      aagacccag aataaaatt caagtcaag ccgatgctg 
      gggaaggcg gtgcagccc aagcaaaat gtagttaaa 
      accatcccg gcttcaccg aaacccatc ccgataaag 
      ggaaggccc ccgaccacc tcgacgtct accaatttt 
      cgcccgacg gagagcaac gtcgagggc ggcttaagc 
      atgggtgca aagccgtcg aagagtatc gatctgact 
      ggcgaagat ggtcgtgcg ttaccggac agccgggaa 
      gtgtccttc aacaagctt caaggacgt acctacccg 
      tcgctcgtc gtagttgca aggccgcat cttcgtgtc 
      aaagatttg gcagcagat cgaagtaaa cttgacgtc 
      aaggtcaaa agtgtgctt atgtatcca cccacgaag 
      tttaccgaa tggctaatc caacaaggg ttggtccga 
      tccgatcaa aaatgttcg gtacatacg acaaatcca 
      ctcaagctg gggatgtat agtgatgcc tcgaagttt 
      ccctattcg gggggttac gtgtggatt tcagagtgt 
      tgcccccag aggtttgtt tcggttttt agtggctct 
      atatttgaa gggtcgcag catccaccg atgtcaatt 
      ttgaaactt ttgtaccat tggtgttgc cagacgatt 
      gtgcaaaac gttactcaa tgggtcaaa gttgataat 
      ctctacgtt aaaggtatg tacacttgg ctgcgctcc 
      gtatgtaca gttgcaata tattcccat attaaacaa 
      ttgggtggg ccgggggtt aaagttcaa atcggggtg 
      ataagtctc gggacaacg tcccaagat ggtaacaag 
      cgagaggtc aaagtggaa gtattggga attctggaa 
      accaccacc agacatata cgactgcga gcggtggaa 
      ccgctaagc gaacttgag cgtaattac aagaaacgg 
      ttctcaaaa atccttgag ccggtgttc aactgggtg 
      cacccgcaa agtatcatt gtaacagat ctgacagtt 
      gacaaaaac acactgcat caactgggt ttcctccac 
      gtccaacaa aacacatcc aacgattat gcgaacagc 
      aaccgtact ataatggag tacctgcga cgcatcgtc 
      ccccgaatg ttccaaaat acgttatct cttctttcc 
      cgacccata atccagcaa ttcctcgac gaattggtc 
      tggagggag tggtacggt accaccgtt ttgcaatgt 
      ttcgacaat atggtcaaa catatctcg gaacagact 
      cggttcgac tcgggttcg tccctcatt aaccggttg 
      aacaaagtc agcgcaaac ccattcaaa aactggtcg 
      attacgacc tacaccact tccactcct tcgaaaccg 
      gtggaaccc gctccaaag cgctacagc cgcttgcgc 
      aaaccgcaa ccctctgca cctgcacca cctccggta 
      caggaggta gtaaggcct agtaaatct cattcgccg 
      gatgtacct gagcagatg gtacctctt gaaaattac 
      tactacggt accatttct ccgactacg ccctccaag 
      aataatacc atgattaat attaagtgc cccttctgt 
      aaattgacg tttaatacg aacatctcc ctcatgaac 
      cacttattc aaacatgcg cataacgtg tcgaatgac 
      gtccaattg tgcaagtac tgtctgacg atagtacca 
      tcagccaat gatctgctc aagcatatt aacaccgcg 
      catcctgac gaaaccaag tttgacaac ggattcgtg 
      tgtttgatt tgcgaatcc cactttcgg aatccgttc 
      atactagga aaacacttg tcaagggaa cacaacccg 
      tcggaacta ccctatttg tgcggtact tgcgggttc 
      cggtgttct aatcataag caagccgtg gatcacttc 
      taccaacag cacgaccgc ggaacgacg attcagtgt 
      ccgttctgt cttaaatcg acgacgatt tactcggga 
      gggaggaac atatcgcaa aatatgaac tacttcatc 
      caacattta caaaaacat cagaaaaaa cagctggcg 
      aaacggtgc ggaaagtgc acgctttgg ttcatccaa 
      aaagagttt cttaaagac catcagatc aaaatgcac 
      gcttcgcag agaggcaaa ccaggactg gtaccagcg 
      tttgcaccg agaaacggc gtcatggtg cccaaatcc 
      aagatggac aagtggcca gatgatgcc aaagtgatc 
      aatttctcg accctttat ttcaacgtt aacaagaat 
      cttaattgt aaagagtgc ggacaatcg ttaagttca 
      ccgaaacac ttcgcgtca tttgatagt tgtcagaat 
      ccaaactgc cagttttcg acatgttgc accacagcc 
      atgcaaaac cacgtggct aaatgtaaa ttcctcaat 
      tcgaaacta ccggccgag aagctcccc tatgagatg 
      ttttgtatt tgtggctac agcgatacc gatggtaac 
      atgttggct aaacatttg gcaatttgc gagcgcaaa 
      tcggcgtat ccatctcag gaggctgcg aaagaggcc 
      accgtcact catagtatg cttgatgtt ttgggtttg 
      gtgcggaaa ccagaagag gcgacgtcg agtaaagat 
      gatctgccc cgacttata ccgacaaga tctgaaccg 
      attattttg aaacgcggc aggcctagg aaacatccg 
      gtaccacca aaagaagtc attgaagta aaagatgac 
      gaagagccc ttgagtatt agtgaggtc aggggaggg 
      gttaaaatt gaaggagag aaacatggt atgactgaa 
      attgaagaa attaaagaa agtgaaaat aaggatgtc 
      tcggagaaa caaaaggaa gtccaagag gataagagt 
      caagttgga caggaagac catcaagag aaaggtact 
      gaagaagaa acaagtgaa gtcgttgat aaggaacaa 
      gaagaccat cctgagaaa gagattgaa gaagaagca 
      agtgaagtc ggtggtaag ggacaagaa gaagatcaa 
      gggaaagag attgaagaa gaaacaagt gaagtggtt 
      gataaggga caagaagac caacaagaa aaagagatt 
      gcagaagaa acaagtgaa gtggttgat aaggaacaa 
      gaagatcaa caagagaaa aatattgaa aaagaaaca 
      agtgaaatc gttaaggca ccagaagac caagaagag 
      aaaaatgtt gaagaagaa acaagtgaa gttattgat 
      aaagtacaa gaagaccct cacgaggaa gaagaaaaa 
      acaaatgac attgttgat aagggtcaa gaagatcat 
      agaaatgag gaagttgaa gaaacgacg agtggaatt 
      aaggaacaa gaagacgtt caacaaaaa gaagctgaa 
      gatgacagt ggaattgtt gataataaa tcggaaaat 
      gatgaagta aagtgtgtg gaagaagag gaaataaaa 
      gaacacagt gcagaagca cgagacaac gaatctgtt 
      agtgaaaat acaaaagac gtaaatgaa ttacagtct 
      gagacagtt gaaagtgaa agtatggaa gttgacagt 
      tgtaatgcc agcgaaaca gtcgaaacg gtggcaata 
      ggaaatcaa atggaaaaa aactctgaa actattgat 
      gatatctgt cctatggat acagactaa
    mRNA sequence
    • >TC014398 RA
      cgtcaaaca taaataaat atggctcct gttgcaaag 
      tcagtaatg tagcgttga cgttttggt gtgttaatt 
      caagtttta agcacttaa catgccgaa agagaaaca 
      gctgaatcg gactcagtc aaggcgggc cgctagtaa 
      ggaggccct gaaacaaat gcatctgtt gctgggagc 
      tgataataa taaatatga aatatcgtc agatgacga 
      tgttataga agtcgaggt gcagccacc aaagaagaa 
      atataagtt aagtcaccg gaagtcatc agatggaaa 
      tgatcgtga cgtcatagt tttaaaacc cgttgacga 
      aagtgtttt cgacttgga agtggtcgt cgaagactt 
      agccccaag tcagaagtc tcgtctcga gttgcttca 
      aaaagaaac aaacgaagt tgaaacaac aattcaaca 
      actcctcac cgcccccaa caccgaagt cgtctacaa 
      tggggtcta cacgaccgc tgtcgaaat cactgaaca 
      gatacagac aatccttag cacgcccac ccccgctgc 
      cgcctccac cgcccagtt cacaatcac gaaccaaac 
      tccgctcgc cgcccccac ccccgtaca acagccggt 
      cgctagtca gagtctagt tttgcagca gcagccacc 
      gtctgccaa caatgcgat tccacaaag ctaccaact 
      ggtcatgga tccgcggct tggtgtcat cgtgggggc 
      ggtgtccac gtcaaacgc tagtcccaa cacgccgcc 
      ccttttgta tcaacccaa agttgcaat tccggcgcc 
      gggggcggc agcagctac agaatcgcc ggcgggcaa 
      gggacggcg cggccccat tttgagaca agtgcccgc 
      taatgctat cccaccgct ggcgaaagt gtcgcagaa 
      gacgcccgt ggcggccaa caagaccca gaataaaat 
      tcaagtcaa gccgatgct ggggaaggc ggtgcagcc 
      caagcaaaa tgtagttaa aaccatccc ggcttcacc 
      gaaacccat cccgataaa gggaaggcc cccgaccac 
      ctcgacgtc taccaattt tcgcccgac ggagagcaa 
      cgtcgaggg cggcttaag catgggtgc aaagccgtc 
      gaagagtat cgatctgac tggcgaaga tggtcgtgc 
      gttaccgga cagccggga agtgtcctt caacaagct 
      tcaaggacg tacctaccc gtcgctcgt cgtagttgc 
      aaggccgca tcttcgtgt caaagattt ggcagcaga 
      tcgaagtaa acttgacgt caaggtcaa aagtgtgct 
      tatgtatcc acccacgaa gtttaccga atggctaat 
      ccaacaagg gttggtccg atccgatca aaaatgttc 
      ggtacatac gacaaatcc actcaagct ggggatgta 
      tagtgatgc ctcgaagtt tccctattc ggggggtta 
      cgtgtggat ttcagagtg ttgccccca gaggtttgt 
      ttcggtttt tagtggctc tatatttga agggtcgca 
      gcatccacc gatgtcaat tttgaaact tttgtacca 
      ttggtgttg ccagacgat tgtgcaaaa cgttactca 
      atgggtcaa agttgataa tctctacgt taaaggtat 
      gtacacttg gctgcgctc cgtatgtac agttgcaat 
      atattccca tattaaaca attgggtgg gccgggggt 
      taaagttca aatcggggt gataagtct cgggacaac 
      gtcccaaga tggtaacaa gcgagaggt caaagtgga 
      agtattggg aattctgga aaccaccac cagacatat 
      acgactgcg agcggtgga accgctaag cgaacttga 
      gcgtaatta caagaaacg gttctcaaa aatccttga 
      gccggtgtt caactgggt gcacccgca aagtatcat 
      tgtaacaga tctgacagt tgacaaaaa cacactgca 
      tcaactggg tttcctcca cgtccaaca aaacacatc 
      caacgatta tgcgaacag caaccgtac tataatgga 
      gtacctgcg acgcatcgt cccccgaat gttccaaaa 
      tacgttatc tcttctttc ccgacccat aatccagca 
      attcctcga cgaattggt ctggaggga gtggtacgg 
      taccaccgt tttgcaatg tttcgacaa tatggtcaa 
      acatatctc ggaacagac tcggttcga ctcgggttc 
      gtccctcat taaccggtt gaacaaagt cagcgcaaa 
      cccattcaa aaactggtc gattacgac ctacaccac 
      ttccactcc ttcgaaacc ggtggaacc cgctccaaa 
      gcgctacag ccgcttgcg caaaccgca accctctgc 
      acctgcacc acctccggt acaggaggt agtaaggcc 
      tagtaaatc tcattcgcc ggatgtacc tgagcagat 
      ggtacctct tgaaaatta ctactacgg taccatttc 
      tccgactac gccctccaa gaataatac catgattaa 
      tattaagtg ccccttctg taaattgac gtttaatac 
      gaacatctc cctcatgaa ccacttatt caaacatgc 
      gcataacgt gtcgaatga cgtccaatt gtgcaagta 
      ctgtctgac gatagtacc atcagccaa tgatctgct 
      caagcatat taacaccgc gcatcctga cgaaaccaa 
      gtttgacaa cggattcgt gtgtttgat ttgcgaatc 
      ccactttcg gaatccgtt catactagg aaaacactt 
      gtcaaggga acacaaccc gtcggaact accctattt 
      gtgcggtac ttgcgggtt ccggtgttc taatcataa 
      gcaagccgt ggatcactt ctaccaaca gcacgaccg 
      cggaacgac gattcagtg tccgttctg tcttaaatc 
      gacgacgat ttactcggg agggaggaa catatcgca 
      aaatatgaa ctacttcat ccaacattt acaaaaaca 
      tcagaaaaa acagctggc gaaacggtg cggaaagtg 
      cacgctttg gttcatcca aaaagagtt tcttaaaga 
      ccatcagat caaaatgca cgcttcgca gagaggcaa 
      accaggact ggtaccagc gtttgcacc gagaaacgg 
      cgtcatggt gcccaaatc caagatgga caagtggcc 
      agatgatgc caaagtgat caatttctc gacccttta 
      tttcaacgt taacaagaa tcttaattg taaagagtg 
      cggacaatc gttaagttc accgaaaca cttcgcgtc 
      atttgatag ttgtcagaa tccaaactg ccagttttc 
      gacatgttg caccacagc catgcaaaa ccacgtggc 
      taaatgtaa attcctcaa ttcgaaact accggccga 
      gaagctccc ctatgagat gttttgtat ttgtggcta 
      cagcgatac cgatggtaa catgttggc taaacattt 
      ggcaatttg cgagcgcaa atcggcgta tccatctca 
      ggaggctgc gaaagaggc caccgtcac tcatagtat 
      gcttgatgt tttgggttt ggtgcggaa accagaaga 
      ggcgacgtc gagtaaaga tgatctgcc ccgacttat 
      accgacaag atctgaacc gattatttt gaaacgcgg 
      caggcctag gaaacatcc ggtaccacc aaaagaagt 
      cattgaagt aaaagatga cgaagagcc cttgagtat 
      tagtgaggt caggggagg ggttaaaat tgaaggaga 
      gaaacatgg tatgactga aattgaaga aattaaaga 
      aagtgaaaa taaggatgt ctcggagaa acaaaagga 
      agtccaaga ggataagag tcaagttgg acaggaaga 
      ccatcaaga gaaaggtac tgaagaaga aacaagtga 
      agtcgttga taaggaaca agaagacca tcctgagaa 
      agagattga agaagaagc aagtgaagt cggtggtaa 
      gggacaaga agaagatca agggaaaga gattgaaga 
      agaaacaag tgaagtggt tgataaggg acaagaaga 
      ccaacaaga aaaagagat tgcagaaga aacaagtga 
      agtggttga taaggaaca agaagatca acaagagaa 
      aaatattga aaaagaaac aagtgaaat cgttaaggc 
      accagaaga ccaagaaga gaaaaatgt tgaagaaga 
      aacaagtga agttattga taaagtaca agaagaccc 
      tcacgagga agaagaaaa aacaaatga cattgttga 
      taagggtca agaagatca tagaaatga ggaagttga 
      agaaacgac gagtggaat taaggaaca agaagacgt 
      tcaacaaaa agaagctga agatgacag tggaattgt 
      tgataataa atcggaaaa tgatgaagt aaagtgtgt 
      ggaagaaga ggaaataaa agaacacag tgcagaagc 
      acgagacaa cgaatctgt tagtgaaaa tacaaaaga 
      cgtaaatga attacagtc tgagacagt tgaaagtga 
      aagtatgga agttgacag ttgtaatgc cagcgaaac 
      agtcgaaac ggtggcaat aggaaatca aatggaaaa 
      aaactctga aactattga tgatatctg tcctatgga 
      tacagacta aaacctaga gagtatatt acgtttgta 
      aataagatg ttattgttc aagactgta aaaagtaat 
      tgttgcgaa catagttaa ttagtgttt gcgtttctt 
      tcaaactcg tttttcttt tacaaaagc agatttttt 
      atatgctta tcacgtctg ttctgtaag gttctattg 
      aatattata tttgtaata cttttaaat aaaaaaatc 
      ctccttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY