| General information | |||
| TC014398 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10061193..10067180 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014398 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05624 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)remarks: later than schedule, hours 48
tropharium structure irregular - Number of animals showing the phenotype: 3
vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 3
mature egg potentially degenerated - Number of animals showing the phenotype: 1
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014398 RA
MPKEKQLNR TQSRRAASK EALKQMHLL LGADNNKYE ISSDDDVIE VEVQPPKKK YKLSHRKSS DGNDRDVIV LKPVDESVF DLEVVVEDL APSQKSRLE LLQKETNEV ETTIQQLLT APNTEVVYN GVYTTAVEI TEQIQTILS TPTPAAAST AQFTITNQT PLAAPTPVQ QPVASQSLV LQQQPPSAN NAIPQSYQL VMDPRLGVI VGAVSTSNA SPNTPPLLY QPKVAIPAP GAAAATESP AGKGRRGPI LRQVPANAI PPLAKVSQK TPVAANKTQ NKIQVKPML GKAVQPKQN VVKTIPASP KPIPIKGRP PTTSTSTNF RPTESNVEG GLSMGAKPS KSIDLTGED GRALPDSRE VSFNKLQGR TYPSLVVVA RPHLRVKDL AADRSKLDV KVKSVLMYP PTKFTEWLI QQGLVRSDQ KCSVHTTNP LKLGMYSDA SKFPYSGGY VWISECCPQ RFVSVFSGS IFEGSQHPP MSILKLLYH WCCQTIVQN VTQWVKVDN LYVKGMYTW LRSVCTVAI YSHIKQLGG PGVKVQIGV ISLGTTSQD GNKREVKVE VLGILETTT RHIRLRAVE PLSELERNY KKRFSKILE PVFNWVHPQ SIIVTDLTV DKNTLHQLG FLHVQQNTS NDYANSNRT IMEYLRRIV PRMFQNTLS LLSRPIIQQ FLDELVWRE WYGTTVLQC FDNMVKHIS EQTRFDSGS SLINRLNKV SANPFKNWS ITTYTTSTP SKPVEPAPK RYSRLRKPQ PSAPAPPPV QEVVRPSKS HSPDVPEQM VPLENYYYG TISPTTPSK NNTMINIKC PFCKLTFNT NISLMNHLF KHAHNVSND VQLCKYCLT IVPSANDLL KHINTAHPD ETKFDNGFV CLICESHFR NPFILGKHL SREHNPSEL PYLCGTCGF RCSNHKQAV DHFYQQHDR GTTIQCPFC LKSTTIYSG GRNISQNMN YFIQHLQKH QKKQLAKRC GKCTLWFIQ KEFLKDHQI KMHASQRGK PGLVPAFAP RNGVMVPKS KMDKWPDDA KVINFSTLY FNVNKNLNC KECGQSLSS PKHFASFDS CQNPNCQFS TCCTTAMQN HVAKCKFLN SKLPAEKLP YEMFCICGY SDTDGNMLA KHLAICERK SAYPSQEAA KEATVTHSM LDVLGLVRK PEEATSSKD DLPRLIPTR SEPIILKRG RPRKHPVPP KEVIEVKDD EEPLSISEV RGGVKIEGE KHGMTEIEE IKESENKDV SEKQKEVQE DKSQVGQED HQEKGTEEE TSEVVDKEQ EDHPEKEIE EEASEVGGK GQEEDQGKE IEEETSEVV DKGQEDQQE KEIAEETSE VVDKEQEDQ QEKNIEKET SEIVKAPED QEEKNVEEE TSEVIDKVQ EDPHEEEEK TNDIVDKGQ EDHRNEEVE ETTSGIKEQ EDVQQKEAE DDSGIVDNK SENDEVKCV EEEEIKEHS AEARDNESV SENTKDVNE LQSETVESE SMEVDSCNA SETVETVAI GNQMEKNSE TIDDICPMD TD
- >TC014398 RA
atgccgaaa gagaaacag ctgaatcgg actcagtca aggcgggcc gctagtaag gaggccctg aaacaaatg catctgttg ctgggagct gataataat aaatatgaa atatcgtca gatgacgat gttatagaa gtcgaggtg cagccacca aagaagaaa tataagtta agtcaccgg aagtcatca gatggaaat gatcgtgac gtcatagtt ttaaaaccc gttgacgaa agtgttttc gacttggaa gtggtcgtc gaagactta gccccaagt cagaagtct cgtctcgag ttgcttcaa aaagaaaca aacgaagtt gaaacaaca attcaacaa ctcctcacc gcccccaac accgaagtc gtctacaat ggggtctac acgaccgct gtcgaaatc actgaacag atacagaca atccttagc acgcccacc cccgctgcc gcctccacc gcccagttc acaatcacg aaccaaact ccgctcgcc gcccccacc cccgtacaa cagccggtc gctagtcag agtctagtt ttgcagcag cagccaccg tctgccaac aatgcgatt ccacaaagc taccaactg gtcatggat ccgcggctt ggtgtcatc gtgggggcg gtgtccacg tcaaacgct agtcccaac acgccgccc cttttgtat caacccaaa gttgcaatt ccggcgccg ggggcggca gcagctaca gaatcgccg gcgggcaag ggacggcgc ggccccatt ttgagacaa gtgcccgct aatgctatc ccaccgctg gcgaaagtg tcgcagaag acgcccgtg gcggccaac aagacccag aataaaatt caagtcaag ccgatgctg gggaaggcg gtgcagccc aagcaaaat gtagttaaa accatcccg gcttcaccg aaacccatc ccgataaag ggaaggccc ccgaccacc tcgacgtct accaatttt cgcccgacg gagagcaac gtcgagggc ggcttaagc atgggtgca aagccgtcg aagagtatc gatctgact ggcgaagat ggtcgtgcg ttaccggac agccgggaa gtgtccttc aacaagctt caaggacgt acctacccg tcgctcgtc gtagttgca aggccgcat cttcgtgtc aaagatttg gcagcagat cgaagtaaa cttgacgtc aaggtcaaa agtgtgctt atgtatcca cccacgaag tttaccgaa tggctaatc caacaaggg ttggtccga tccgatcaa aaatgttcg gtacatacg acaaatcca ctcaagctg gggatgtat agtgatgcc tcgaagttt ccctattcg gggggttac gtgtggatt tcagagtgt tgcccccag aggtttgtt tcggttttt agtggctct atatttgaa gggtcgcag catccaccg atgtcaatt ttgaaactt ttgtaccat tggtgttgc cagacgatt gtgcaaaac gttactcaa tgggtcaaa gttgataat ctctacgtt aaaggtatg tacacttgg ctgcgctcc gtatgtaca gttgcaata tattcccat attaaacaa ttgggtggg ccgggggtt aaagttcaa atcggggtg ataagtctc gggacaacg tcccaagat ggtaacaag cgagaggtc aaagtggaa gtattggga attctggaa accaccacc agacatata cgactgcga gcggtggaa ccgctaagc gaacttgag cgtaattac aagaaacgg ttctcaaaa atccttgag ccggtgttc aactgggtg cacccgcaa agtatcatt gtaacagat ctgacagtt gacaaaaac acactgcat caactgggt ttcctccac gtccaacaa aacacatcc aacgattat gcgaacagc aaccgtact ataatggag tacctgcga cgcatcgtc ccccgaatg ttccaaaat acgttatct cttctttcc cgacccata atccagcaa ttcctcgac gaattggtc tggagggag tggtacggt accaccgtt ttgcaatgt ttcgacaat atggtcaaa catatctcg gaacagact cggttcgac tcgggttcg tccctcatt aaccggttg aacaaagtc agcgcaaac ccattcaaa aactggtcg attacgacc tacaccact tccactcct tcgaaaccg gtggaaccc gctccaaag cgctacagc cgcttgcgc aaaccgcaa ccctctgca cctgcacca cctccggta caggaggta gtaaggcct agtaaatct cattcgccg gatgtacct gagcagatg gtacctctt gaaaattac tactacggt accatttct ccgactacg ccctccaag aataatacc atgattaat attaagtgc cccttctgt aaattgacg tttaatacg aacatctcc ctcatgaac cacttattc aaacatgcg cataacgtg tcgaatgac gtccaattg tgcaagtac tgtctgacg atagtacca tcagccaat gatctgctc aagcatatt aacaccgcg catcctgac gaaaccaag tttgacaac ggattcgtg tgtttgatt tgcgaatcc cactttcgg aatccgttc atactagga aaacacttg tcaagggaa cacaacccg tcggaacta ccctatttg tgcggtact tgcgggttc cggtgttct aatcataag caagccgtg gatcacttc taccaacag cacgaccgc ggaacgacg attcagtgt ccgttctgt cttaaatcg acgacgatt tactcggga gggaggaac atatcgcaa aatatgaac tacttcatc caacattta caaaaacat cagaaaaaa cagctggcg aaacggtgc ggaaagtgc acgctttgg ttcatccaa aaagagttt cttaaagac catcagatc aaaatgcac gcttcgcag agaggcaaa ccaggactg gtaccagcg tttgcaccg agaaacggc gtcatggtg cccaaatcc aagatggac aagtggcca gatgatgcc aaagtgatc aatttctcg accctttat ttcaacgtt aacaagaat cttaattgt aaagagtgc ggacaatcg ttaagttca ccgaaacac ttcgcgtca tttgatagt tgtcagaat ccaaactgc cagttttcg acatgttgc accacagcc atgcaaaac cacgtggct aaatgtaaa ttcctcaat tcgaaacta ccggccgag aagctcccc tatgagatg ttttgtatt tgtggctac agcgatacc gatggtaac atgttggct aaacatttg gcaatttgc gagcgcaaa tcggcgtat ccatctcag gaggctgcg aaagaggcc accgtcact catagtatg cttgatgtt ttgggtttg gtgcggaaa ccagaagag gcgacgtcg agtaaagat gatctgccc cgacttata ccgacaaga tctgaaccg attattttg aaacgcggc aggcctagg aaacatccg gtaccacca aaagaagtc attgaagta aaagatgac gaagagccc ttgagtatt agtgaggtc aggggaggg gttaaaatt gaaggagag aaacatggt atgactgaa attgaagaa attaaagaa agtgaaaat aaggatgtc tcggagaaa caaaaggaa gtccaagag gataagagt caagttgga caggaagac catcaagag aaaggtact gaagaagaa acaagtgaa gtcgttgat aaggaacaa gaagaccat cctgagaaa gagattgaa gaagaagca agtgaagtc ggtggtaag ggacaagaa gaagatcaa gggaaagag attgaagaa gaaacaagt gaagtggtt gataaggga caagaagac caacaagaa aaagagatt gcagaagaa acaagtgaa gtggttgat aaggaacaa gaagatcaa caagagaaa aatattgaa aaagaaaca agtgaaatc gttaaggca ccagaagac caagaagag aaaaatgtt gaagaagaa acaagtgaa gttattgat aaagtacaa gaagaccct cacgaggaa gaagaaaaa acaaatgac attgttgat aagggtcaa gaagatcat agaaatgag gaagttgaa gaaacgacg agtggaatt aaggaacaa gaagacgtt caacaaaaa gaagctgaa gatgacagt ggaattgtt gataataaa tcggaaaat gatgaagta aagtgtgtg gaagaagag gaaataaaa gaacacagt gcagaagca cgagacaac gaatctgtt agtgaaaat acaaaagac gtaaatgaa ttacagtct gagacagtt gaaagtgaa agtatggaa gttgacagt tgtaatgcc agcgaaaca gtcgaaacg gtggcaata ggaaatcaa atggaaaaa aactctgaa actattgat gatatctgt cctatggat acagactaa
- >TC014398 RA
cgtcaaaca taaataaat atggctcct gttgcaaag tcagtaatg tagcgttga cgttttggt gtgttaatt caagtttta agcacttaa catgccgaa agagaaaca gctgaatcg gactcagtc aaggcgggc cgctagtaa ggaggccct gaaacaaat gcatctgtt gctgggagc tgataataa taaatatga aatatcgtc agatgacga tgttataga agtcgaggt gcagccacc aaagaagaa atataagtt aagtcaccg gaagtcatc agatggaaa tgatcgtga cgtcatagt tttaaaacc cgttgacga aagtgtttt cgacttgga agtggtcgt cgaagactt agccccaag tcagaagtc tcgtctcga gttgcttca aaaagaaac aaacgaagt tgaaacaac aattcaaca actcctcac cgcccccaa caccgaagt cgtctacaa tggggtcta cacgaccgc tgtcgaaat cactgaaca gatacagac aatccttag cacgcccac ccccgctgc cgcctccac cgcccagtt cacaatcac gaaccaaac tccgctcgc cgcccccac ccccgtaca acagccggt cgctagtca gagtctagt tttgcagca gcagccacc gtctgccaa caatgcgat tccacaaag ctaccaact ggtcatgga tccgcggct tggtgtcat cgtgggggc ggtgtccac gtcaaacgc tagtcccaa cacgccgcc ccttttgta tcaacccaa agttgcaat tccggcgcc gggggcggc agcagctac agaatcgcc ggcgggcaa gggacggcg cggccccat tttgagaca agtgcccgc taatgctat cccaccgct ggcgaaagt gtcgcagaa gacgcccgt ggcggccaa caagaccca gaataaaat tcaagtcaa gccgatgct ggggaaggc ggtgcagcc caagcaaaa tgtagttaa aaccatccc ggcttcacc gaaacccat cccgataaa gggaaggcc cccgaccac ctcgacgtc taccaattt tcgcccgac ggagagcaa cgtcgaggg cggcttaag catgggtgc aaagccgtc gaagagtat cgatctgac tggcgaaga tggtcgtgc gttaccgga cagccggga agtgtcctt caacaagct tcaaggacg tacctaccc gtcgctcgt cgtagttgc aaggccgca tcttcgtgt caaagattt ggcagcaga tcgaagtaa acttgacgt caaggtcaa aagtgtgct tatgtatcc acccacgaa gtttaccga atggctaat ccaacaagg gttggtccg atccgatca aaaatgttc ggtacatac gacaaatcc actcaagct ggggatgta tagtgatgc ctcgaagtt tccctattc ggggggtta cgtgtggat ttcagagtg ttgccccca gaggtttgt ttcggtttt tagtggctc tatatttga agggtcgca gcatccacc gatgtcaat tttgaaact tttgtacca ttggtgttg ccagacgat tgtgcaaaa cgttactca atgggtcaa agttgataa tctctacgt taaaggtat gtacacttg gctgcgctc cgtatgtac agttgcaat atattccca tattaaaca attgggtgg gccgggggt taaagttca aatcggggt gataagtct cgggacaac gtcccaaga tggtaacaa gcgagaggt caaagtgga agtattggg aattctgga aaccaccac cagacatat acgactgcg agcggtgga accgctaag cgaacttga gcgtaatta caagaaacg gttctcaaa aatccttga gccggtgtt caactgggt gcacccgca aagtatcat tgtaacaga tctgacagt tgacaaaaa cacactgca tcaactggg tttcctcca cgtccaaca aaacacatc caacgatta tgcgaacag caaccgtac tataatgga gtacctgcg acgcatcgt cccccgaat gttccaaaa tacgttatc tcttctttc ccgacccat aatccagca attcctcga cgaattggt ctggaggga gtggtacgg taccaccgt tttgcaatg tttcgacaa tatggtcaa acatatctc ggaacagac tcggttcga ctcgggttc gtccctcat taaccggtt gaacaaagt cagcgcaaa cccattcaa aaactggtc gattacgac ctacaccac ttccactcc ttcgaaacc ggtggaacc cgctccaaa gcgctacag ccgcttgcg caaaccgca accctctgc acctgcacc acctccggt acaggaggt agtaaggcc tagtaaatc tcattcgcc ggatgtacc tgagcagat ggtacctct tgaaaatta ctactacgg taccatttc tccgactac gccctccaa gaataatac catgattaa tattaagtg ccccttctg taaattgac gtttaatac gaacatctc cctcatgaa ccacttatt caaacatgc gcataacgt gtcgaatga cgtccaatt gtgcaagta ctgtctgac gatagtacc atcagccaa tgatctgct caagcatat taacaccgc gcatcctga cgaaaccaa gtttgacaa cggattcgt gtgtttgat ttgcgaatc ccactttcg gaatccgtt catactagg aaaacactt gtcaaggga acacaaccc gtcggaact accctattt gtgcggtac ttgcgggtt ccggtgttc taatcataa gcaagccgt ggatcactt ctaccaaca gcacgaccg cggaacgac gattcagtg tccgttctg tcttaaatc gacgacgat ttactcggg agggaggaa catatcgca aaatatgaa ctacttcat ccaacattt acaaaaaca tcagaaaaa acagctggc gaaacggtg cggaaagtg cacgctttg gttcatcca aaaagagtt tcttaaaga ccatcagat caaaatgca cgcttcgca gagaggcaa accaggact ggtaccagc gtttgcacc gagaaacgg cgtcatggt gcccaaatc caagatgga caagtggcc agatgatgc caaagtgat caatttctc gacccttta tttcaacgt taacaagaa tcttaattg taaagagtg cggacaatc gttaagttc accgaaaca cttcgcgtc atttgatag ttgtcagaa tccaaactg ccagttttc gacatgttg caccacagc catgcaaaa ccacgtggc taaatgtaa attcctcaa ttcgaaact accggccga gaagctccc ctatgagat gttttgtat ttgtggcta cagcgatac cgatggtaa catgttggc taaacattt ggcaatttg cgagcgcaa atcggcgta tccatctca ggaggctgc gaaagaggc caccgtcac tcatagtat gcttgatgt tttgggttt ggtgcggaa accagaaga ggcgacgtc gagtaaaga tgatctgcc ccgacttat accgacaag atctgaacc gattatttt gaaacgcgg caggcctag gaaacatcc ggtaccacc aaaagaagt cattgaagt aaaagatga cgaagagcc cttgagtat tagtgaggt caggggagg ggttaaaat tgaaggaga gaaacatgg tatgactga aattgaaga aattaaaga aagtgaaaa taaggatgt ctcggagaa acaaaagga agtccaaga ggataagag tcaagttgg acaggaaga ccatcaaga gaaaggtac tgaagaaga aacaagtga agtcgttga taaggaaca agaagacca tcctgagaa agagattga agaagaagc aagtgaagt cggtggtaa gggacaaga agaagatca agggaaaga gattgaaga agaaacaag tgaagtggt tgataaggg acaagaaga ccaacaaga aaaagagat tgcagaaga aacaagtga agtggttga taaggaaca agaagatca acaagagaa aaatattga aaaagaaac aagtgaaat cgttaaggc accagaaga ccaagaaga gaaaaatgt tgaagaaga aacaagtga agttattga taaagtaca agaagaccc tcacgagga agaagaaaa aacaaatga cattgttga taagggtca agaagatca tagaaatga ggaagttga agaaacgac gagtggaat taaggaaca agaagacgt tcaacaaaa agaagctga agatgacag tggaattgt tgataataa atcggaaaa tgatgaagt aaagtgtgt ggaagaaga ggaaataaa agaacacag tgcagaagc acgagacaa cgaatctgt tagtgaaaa tacaaaaga cgtaaatga attacagtc tgagacagt tgaaagtga aagtatgga agttgacag ttgtaatgc cagcgaaac agtcgaaac ggtggcaat aggaaatca aatggaaaa aaactctga aactattga tgatatctg tcctatgga tacagacta aaacctaga gagtatatt acgtttgta aataagatg ttattgttc aagactgta aaaagtaat tgttgcgaa catagttaa ttagtgttt gcgtttctt tcaaactcg tttttcttt tacaaaagc agatttttt atatgctta tcacgtctg ttctgtaag gttctattg aatattata tttgtaata cttttaaat aaaaaaatc ctccttga
