General information | |||
TC014393 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 10014435..10017211 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014393 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05622 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
bristle partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC014393 RA
MEGGPNIQE ADDTINSST AEEGGNSGC YYSCCDPRG RLHRFIALI FMCFLGFGS YFCYDNPAA LEGHIEKTL NITTTQYSL LYSMYSWPN VVLCFVGGF LIDRVFGIR LGTNIYSGL TLLGQIIFA SGALFDVFW IMIMGRFIF GIGAESLAV AQNNYAVLW FKGKELNMV FGLQLSFAR IGSTVNFLV MESIYQWVN KEYKGTELL GIVLFVATS TCLFSMLCA LVLGFMDKR AENLLRRHE TTNPEVVRF TDVIEFKFT FWLIAVICV SYYVAIFPF IALGEDLFI KKFHMSKQE ANTINSLIY LISGVASPL VGLFIDKVG KNILWIVVA ISVTIGSHT LWAFTDVNA YVGMVILGL AYACLASSL WPLVSLIIP EYQLGTAYG VCQAIQNLG LAVFTIVAG HIKDTYGYI WLEVFFICT LIVALVFTL VLWLVDMYQ RGVLNMTPG NRVAHQERL SSAADAEKQ RLLSSEASS SNDNDNLQP SSDFEIRNR YLSRIGAPV SDNTEE
- >TC014393 RB
MEGGPNIQE ADDTINSST AEEGGNSGC YYSCCDPRG RLHRFIALI FMCFLGFGS YFCYDNPAA LEGHIEKTL NITTTQYSL LYSMYSWPN VVLCFVGGF LIDRVFGIR LGTNIYSGL TLLGQIIFA SGALFDVFW IMIMGRFIF GIGAESLAV AQNNYAVLW FKGKELNMV FGLQLSFAR IGSTVNFLV MESIYQWVN KEYKGTELL GIVLFVATS TCLFSMLCA LVLGFMDKR AENLLRRHE TTNPEVVRF TDVIEFKFT FWLIAVICV SYYVAIFPF IALGEDLFI KKFHMSKQE ANTINSLIY LISGVASPL VGLFIDKVG KNILWIVVA ISVTIGSHT LWAFTDVNA YVGMVILGL AYACLASSL WPLVSLIIP EYQLGTAYG VCQAIQNLG LAVFTIVAG HIKDTYGYI WLEVFFICT LIVALVFTL VLWLVDMYQ RGVLNMTPG NRVAHQERL SSAADAEKQ RLLSSEASS SNDNDNLQP SSDFEIRNR YLSRIGAPL PDHYSIQNK GLSYRALR
- >TC014393 RA
atggagggt ggacccaac atacaagaa gcagacgac acaataaat tcctccact gctgaggaa ggaggaaat tcgggatgt tactacagc tgctgtgac cccaggggc aggctgcac cgctttata gccctaatt ttcatgtgc ttcctcggt tttggttcc tatttctgt tacgacaac cctgcagct ttagaaggc cacatcgaa aaaacctta aatataacc acaactcaa tattcccta ctttactcg atgtactct tggccaaac gttgtgcta tgtttcgtt ggaggcttt ctaattgac cgagttttc ggaattcgt ctcggcacc aatatttat tcgggcctc acgttgttg gggcaaatt attttcgcc agcggggcg ctgtttgac gttttttgg ataatgatc atgggcaga tttattttt ggcataggg gctgagtcg ctagcagtc gcacaaaat aattacgcc gtgttgtgg ttcaaaggc aaagaactt aacatggtt tttgggctg cagttgagt tttgcgcgg attggtagc actgtgaat tttctcgtg atggaatcg atttaccag tgggttaat aaggagtac aaagggacg gaattgcta ggaattgtt ttatttgtg gccacttcg acctgtctg ttctccatg ctttgtgcc ctagttttg ggttttatg gacaagcgg gcggaaaat ctgttaaga cgacacgaa accacaaac cctgaagtt gttcgattt accgacgtt attgagttt aaattcacc ttttggctt attgccgtg atctgtgtt tcttattac gtcgctata tttcccttt atcgccctt ggagaagac ctatttatt aaaaaattc cacatgtcg aaacaagag gcgaacacg attaatagt ctcatttac ttgatatca ggagtggct tcgcccctc gtgggcctt tttatcgac aaagttggc aaaaatatt ttatggatc gttgtggca atttcagtg acgataggg tcacacacc ttgtgggcc tttaccgac gtcaacgcc tacgttgga atggttatc ttggggctt gcatacgca tgcttggcc agtagttta tggcctttg gtctctttg ataattccc gagtatcag ttaggaaca gcttacgga gtttgtcaa gctattcaa aatcttggc ctggccgtg tttacaata gtggctggg cacatcaaa gacacctac ggctacatc tggcttgaa gtgttcttc atttgcaca ctaatagtc gccttagtg ttcactctt gttttatgg ttggttgac atgtaccaa agaggtgtc ctgaacatg actcccgga aatcgggta gcgcatcaa gagaggcta tcaagcgcc gcagacgcg gagaaacaa agactctta tcgtctgaa gcgagttca agtaacgac aatgataat ttacaaccg agttcagat tttgaaatt aggaaccga tatttgtcg aggatcggg gcaccagta agtgacaac acggaagaa tga
- >TC014393 RB
atggagggt ggacccaac atacaagaa gcagacgac acaataaat tcctccact gctgaggaa ggaggaaat tcgggatgt tactacagc tgctgtgac cccaggggc aggctgcac cgctttata gccctaatt ttcatgtgc ttcctcggt tttggttcc tatttctgt tacgacaac cctgcagct ttagaaggc cacatcgaa aaaacctta aatataacc acaactcaa tattcccta ctttactcg atgtactct tggccaaac gttgtgcta tgtttcgtt ggaggcttt ctaattgac cgagttttc ggaattcgt ctcggcacc aatatttat tcgggcctc acgttgttg gggcaaatt attttcgcc agcggggcg ctgtttgac gttttttgg ataatgatc atgggcaga tttattttt ggcataggg gctgagtcg ctagcagtc gcacaaaat aattacgcc gtgttgtgg ttcaaaggc aaagaactt aacatggtt tttgggctg cagttgagt tttgcgcgg attggtagc actgtgaat tttctcgtg atggaatcg atttaccag tgggttaat aaggagtac aaagggacg gaattgcta ggaattgtt ttatttgtg gccacttcg acctgtctg ttctccatg ctttgtgcc ctagttttg ggttttatg gacaagcgg gcggaaaat ctgttaaga cgacacgaa accacaaac cctgaagtt gttcgattt accgacgtt attgagttt aaattcacc ttttggctt attgccgtg atctgtgtt tcttattac gtcgctata tttcccttt atcgccctt ggagaagac ctatttatt aaaaaattc cacatgtcg aaacaagag gcgaacacg attaatagt ctcatttac ttgatatca ggagtggct tcgcccctc gtgggcctt tttatcgac aaagttggc aaaaatatt ttatggatc gttgtggca atttcagtg acgataggg tcacacacc ttgtgggcc tttaccgac gtcaacgcc tacgttgga atggttatc ttggggctt gcatacgca tgcttggcc agtagttta tggcctttg gtctctttg ataattccc gagtatcag ttaggaaca gcttacgga gtttgtcaa gctattcaa aatcttggc ctggccgtg tttacaata gtggctggg cacatcaaa gacacctac ggctacatc tggcttgaa gtgttcttc atttgcaca ctaatagtc gccttagtg ttcactctt gttttatgg ttggttgac atgtaccaa agaggtgtc ctgaacatg actcccgga aatcgggta gcgcatcaa gagaggcta tcaagcgcc gcagacgcg gagaaacaa agactctta tcgtctgaa gcgagttca agtaacgac aatgataat ttacaaccg agttcagat tttgaaatt aggaaccga tatttgtcg aggatcggg gcaccatta cctgatcac tattcaatt caaaacaaa ggactctct tacagagcg ttgagataa
- >TC014393 RA
gtccaccaa ccaaacaac aaaaaagta tccaaacac tcaaaatta tgtcaataa aaaggaata aataaataa taaaaattg tgttatgcg ataatacca ttataatat caccatatc tcgctatga ttatgattt gtgcgaaac atttgagac aataaaccg cgacattat cactttcag aaggtcgtc cgtgtctcc caagtgaaa cacaaaaga ggttatttg gggggtgtc agcatgaat accagtgtt ggattaaat taaattgga ctacgagta agtgtgttt ctatttcat tgaacaacc ccacgaaaa tggagggtg gacccaaca tacaagaag cagacgaca caataaatt cctccactg ctgaggaag gaggaaatt cgggatgtt actacagct gctgtgacc ccaggggca ggctgcacc gctttatag ccctaattt tcatgtgct tcctcggtt ttggttcct atttctgtt acgacaacc ctgcagctt tagaaggcc acatcgaaa aaaccttaa atataacca caactcaat attccctac tttactcga tgtactctt ggccaaacg ttgtgctat gtttcgttg gaggctttc taattgacc gagttttcg gaattcgtc tcggcacca atatttatt cgggcctca cgttgttgg ggcaaatta ttttcgcca gcggggcgc tgtttgacg ttttttgga taatgatca tgggcagat ttatttttg gcatagggg ctgagtcgc tagcagtcg cacaaaata attacgccg tgttgtggt tcaaaggca aagaactta acatggttt ttgggctgc agttgagtt ttgcgcgga ttggtagca ctgtgaatt ttctcgtga tggaatcga tttaccagt gggttaata aggagtaca aagggacgg aattgctag gaattgttt tatttgtgg ccacttcga cctgtctgt tctccatgc tttgtgccc tagttttgg gttttatgg acaagcggg cggaaaatc tgttaagac gacacgaaa ccacaaacc ctgaagttg ttcgattta ccgacgtta ttgagttta aattcacct tttggctta ttgccgtga tctgtgttt cttattacg tcgctatat ttcccttta tcgcccttg gagaagacc tatttatta aaaaattcc acatgtcga aacaagagg cgaacacga ttaatagtc tcatttact tgatatcag gagtggctt cgcccctcg tgggccttt ttatcgaca aagttggca aaaatattt tatggatcg ttgtggcaa tttcagtga cgatagggt cacacacct tgtgggcct ttaccgacg tcaacgcct acgttggaa tggttatct tggggcttg catacgcat gcttggcca gtagtttat ggcctttgg tctctttga taattcccg agtatcagt taggaacag cttacggag tttgtcaag ctattcaaa atcttggcc tggccgtgt ttacaatag tggctgggc acatcaaag acacctacg gctacatct ggcttgaag tgttcttca tttgcacac taatagtcg ccttagtgt tcactcttg ttttatggt tggttgaca tgtaccaaa gaggtgtcc tgaacatga ctcccggaa atcgggtag cgcatcaag agaggctat caagcgccg cagacgcgg agaaacaaa gactcttat cgtctgaag cgagttcaa gtaacgaca atgataatt tacaaccga gttcagatt ttgaaatta ggaaccgat atttgtcga ggatcgggg caccagtaa gtgacaaca cggaagaat gaaaaggat gagaatagt taaatattt tttgtttca gttacctga tcactattc aattcaaaa caaaggact ctcttacag agcgttgag ataagtctt ttcaattat tatacgatt ttgtgatca aataaagtg ataaaacac aagactaat attgaatac aaaaaatat ttaacgatt ctgtaatga tcaaagcag aatttgaat caaagtgta ttgtgatac atatcgtaa gatactaag taaaaagtg taaaatatt aaaggttta gaacatttt ctactctct gtttccaca gttgtgtac tctatagat caatatatt tattgttag tacggaatt ttatagtgt aattgtaat taataaaga catttgtct att
- >TC014393 RB
gtccaccaa ccaaacaac aaaaaagta tccaaacac tcaaaatta tgtcaataa aaaggaata aataaataa taaaaattg tgttatgcg ataatacca ttataatat caccatatc tcgctatga ttatgattt gtgcgaaac atttgagac aataaaccg cgacattat cactttcag aaggtcgtc cgtgtctcc caagtgaaa cacaaaaga ggttatttg gggggtgtc agcatgaat accagtgtt ggattaaat taaattgga ctacgagta agtgtgttt ctatttcat tgaacaacc ccacgaaaa tggagggtg gacccaaca tacaagaag cagacgaca caataaatt cctccactg ctgaggaag gaggaaatt cgggatgtt actacagct gctgtgacc ccaggggca ggctgcacc gctttatag ccctaattt tcatgtgct tcctcggtt ttggttcct atttctgtt acgacaacc ctgcagctt tagaaggcc acatcgaaa aaaccttaa atataacca caactcaat attccctac tttactcga tgtactctt ggccaaacg ttgtgctat gtttcgttg gaggctttc taattgacc gagttttcg gaattcgtc tcggcacca atatttatt cgggcctca cgttgttgg ggcaaatta ttttcgcca gcggggcgc tgtttgacg ttttttgga taatgatca tgggcagat ttatttttg gcatagggg ctgagtcgc tagcagtcg cacaaaata attacgccg tgttgtggt tcaaaggca aagaactta acatggttt ttgggctgc agttgagtt ttgcgcgga ttggtagca ctgtgaatt ttctcgtga tggaatcga tttaccagt gggttaata aggagtaca aagggacgg aattgctag gaattgttt tatttgtgg ccacttcga cctgtctgt tctccatgc tttgtgccc tagttttgg gttttatgg acaagcggg cggaaaatc tgttaagac gacacgaaa ccacaaacc ctgaagttg ttcgattta ccgacgtta ttgagttta aattcacct tttggctta ttgccgtga tctgtgttt cttattacg tcgctatat ttcccttta tcgcccttg gagaagacc tatttatta aaaaattcc acatgtcga aacaagagg cgaacacga ttaatagtc tcatttact tgatatcag gagtggctt cgcccctcg tgggccttt ttatcgaca aagttggca aaaatattt tatggatcg ttgtggcaa tttcagtga cgatagggt cacacacct tgtgggcct ttaccgacg tcaacgcct acgttggaa tggttatct tggggcttg catacgcat gcttggcca gtagtttat ggcctttgg tctctttga taattcccg agtatcagt taggaacag cttacggag tttgtcaag ctattcaaa atcttggcc tggccgtgt ttacaatag tggctgggc acatcaaag acacctacg gctacatct ggcttgaag tgttcttca tttgcacac taatagtcg ccttagtgt tcactcttg ttttatggt tggttgaca tgtaccaaa gaggtgtcc tgaacatga ctcccggaa atcgggtag cgcatcaag agaggctat caagcgccg cagacgcgg agaaacaaa gactcttat cgtctgaag cgagttcaa gtaacgaca atgataatt tacaaccga gttcagatt ttgaaatta ggaaccgat atttgtcga ggatcgggg caccattac ctgatcact attcaattc aaaacaaag gactctctt acagagcgt tgagataag tcttttcaa ttattatac gattttgtg atcaaataa agtgataaa acacaagac taatattga atacaaaaa atatttaac gattctgta atgatcaaa gcagaattt gaatcaaag tgtattgtg atacatatc gtaagatac taagtaaaa agtgtaaaa tattaaagg tttagaaca ttttctact ctctgtttc cacagttgt gtactctat agatcaata tatttattg ttagtacgg aattttata gtgtaattg taattaata aagacattt gtctatt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
