Status
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Gene information
General information
TC014393 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10014435..10017211
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014393
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    bristle partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014393 RA
      MEGGPNIQE ADDTINSST AEEGGNSGC YYSCCDPRG 
RLHRFIALI FMCFLGFGS YFCYDNPAA LEGHIEKTL 
NITTTQYSL LYSMYSWPN VVLCFVGGF LIDRVFGIR 
LGTNIYSGL TLLGQIIFA SGALFDVFW IMIMGRFIF 
GIGAESLAV AQNNYAVLW FKGKELNMV FGLQLSFAR 
IGSTVNFLV MESIYQWVN KEYKGTELL GIVLFVATS 
TCLFSMLCA LVLGFMDKR AENLLRRHE TTNPEVVRF 
TDVIEFKFT FWLIAVICV SYYVAIFPF IALGEDLFI 
KKFHMSKQE ANTINSLIY LISGVASPL VGLFIDKVG 
KNILWIVVA ISVTIGSHT LWAFTDVNA YVGMVILGL 
AYACLASSL WPLVSLIIP EYQLGTAYG VCQAIQNLG 
LAVFTIVAG HIKDTYGYI WLEVFFICT LIVALVFTL 
VLWLVDMYQ RGVLNMTPG NRVAHQERL SSAADAEKQ 
RLLSSEASS SNDNDNLQP SSDFEIRNR YLSRIGAPV 
SDNTEE
    • >TC014393 RB
      MEGGPNIQE ADDTINSST AEEGGNSGC YYSCCDPRG 
RLHRFIALI FMCFLGFGS YFCYDNPAA LEGHIEKTL 
NITTTQYSL LYSMYSWPN VVLCFVGGF LIDRVFGIR 
LGTNIYSGL TLLGQIIFA SGALFDVFW IMIMGRFIF 
GIGAESLAV AQNNYAVLW FKGKELNMV FGLQLSFAR 
IGSTVNFLV MESIYQWVN KEYKGTELL GIVLFVATS 
TCLFSMLCA LVLGFMDKR AENLLRRHE TTNPEVVRF 
TDVIEFKFT FWLIAVICV SYYVAIFPF IALGEDLFI 
KKFHMSKQE ANTINSLIY LISGVASPL VGLFIDKVG 
KNILWIVVA ISVTIGSHT LWAFTDVNA YVGMVILGL 
AYACLASSL WPLVSLIIP EYQLGTAYG VCQAIQNLG 
LAVFTIVAG HIKDTYGYI WLEVFFICT LIVALVFTL 
VLWLVDMYQ RGVLNMTPG NRVAHQERL SSAADAEKQ 
RLLSSEASS SNDNDNLQP SSDFEIRNR YLSRIGAPL 
PDHYSIQNK GLSYRALR
    CDS sequence
    • >TC014393 RA
      atggagggt ggacccaac atacaagaa gcagacgac 
acaataaat tcctccact gctgaggaa ggaggaaat 
tcgggatgt tactacagc tgctgtgac cccaggggc 
aggctgcac cgctttata gccctaatt ttcatgtgc 
ttcctcggt tttggttcc tatttctgt tacgacaac 
cctgcagct ttagaaggc cacatcgaa aaaacctta 
aatataacc acaactcaa tattcccta ctttactcg 
atgtactct tggccaaac gttgtgcta tgtttcgtt 
ggaggcttt ctaattgac cgagttttc ggaattcgt 
ctcggcacc aatatttat tcgggcctc acgttgttg 
gggcaaatt attttcgcc agcggggcg ctgtttgac 
gttttttgg ataatgatc atgggcaga tttattttt 
ggcataggg gctgagtcg ctagcagtc gcacaaaat 
aattacgcc gtgttgtgg ttcaaaggc aaagaactt 
aacatggtt tttgggctg cagttgagt tttgcgcgg 
attggtagc actgtgaat tttctcgtg atggaatcg 
atttaccag tgggttaat aaggagtac aaagggacg 
gaattgcta ggaattgtt ttatttgtg gccacttcg 
acctgtctg ttctccatg ctttgtgcc ctagttttg 
ggttttatg gacaagcgg gcggaaaat ctgttaaga 
cgacacgaa accacaaac cctgaagtt gttcgattt 
accgacgtt attgagttt aaattcacc ttttggctt 
attgccgtg atctgtgtt tcttattac gtcgctata 
tttcccttt atcgccctt ggagaagac ctatttatt 
aaaaaattc cacatgtcg aaacaagag gcgaacacg 
attaatagt ctcatttac ttgatatca ggagtggct 
tcgcccctc gtgggcctt tttatcgac aaagttggc 
aaaaatatt ttatggatc gttgtggca atttcagtg 
acgataggg tcacacacc ttgtgggcc tttaccgac 
gtcaacgcc tacgttgga atggttatc ttggggctt 
gcatacgca tgcttggcc agtagttta tggcctttg 
gtctctttg ataattccc gagtatcag ttaggaaca 
gcttacgga gtttgtcaa gctattcaa aatcttggc 
ctggccgtg tttacaata gtggctggg cacatcaaa 
gacacctac ggctacatc tggcttgaa gtgttcttc 
atttgcaca ctaatagtc gccttagtg ttcactctt 
gttttatgg ttggttgac atgtaccaa agaggtgtc 
ctgaacatg actcccgga aatcgggta gcgcatcaa 
gagaggcta tcaagcgcc gcagacgcg gagaaacaa 
agactctta tcgtctgaa gcgagttca agtaacgac 
aatgataat ttacaaccg agttcagat tttgaaatt 
aggaaccga tatttgtcg aggatcggg gcaccagta 
agtgacaac acggaagaa tga
    • >TC014393 RB
      atggagggt ggacccaac atacaagaa gcagacgac 
acaataaat tcctccact gctgaggaa ggaggaaat 
tcgggatgt tactacagc tgctgtgac cccaggggc 
aggctgcac cgctttata gccctaatt ttcatgtgc 
ttcctcggt tttggttcc tatttctgt tacgacaac 
cctgcagct ttagaaggc cacatcgaa aaaacctta 
aatataacc acaactcaa tattcccta ctttactcg 
atgtactct tggccaaac gttgtgcta tgtttcgtt 
ggaggcttt ctaattgac cgagttttc ggaattcgt 
ctcggcacc aatatttat tcgggcctc acgttgttg 
gggcaaatt attttcgcc agcggggcg ctgtttgac 
gttttttgg ataatgatc atgggcaga tttattttt 
ggcataggg gctgagtcg ctagcagtc gcacaaaat 
aattacgcc gtgttgtgg ttcaaaggc aaagaactt 
aacatggtt tttgggctg cagttgagt tttgcgcgg 
attggtagc actgtgaat tttctcgtg atggaatcg 
atttaccag tgggttaat aaggagtac aaagggacg 
gaattgcta ggaattgtt ttatttgtg gccacttcg 
acctgtctg ttctccatg ctttgtgcc ctagttttg 
ggttttatg gacaagcgg gcggaaaat ctgttaaga 
cgacacgaa accacaaac cctgaagtt gttcgattt 
accgacgtt attgagttt aaattcacc ttttggctt 
attgccgtg atctgtgtt tcttattac gtcgctata 
tttcccttt atcgccctt ggagaagac ctatttatt 
aaaaaattc cacatgtcg aaacaagag gcgaacacg 
attaatagt ctcatttac ttgatatca ggagtggct 
tcgcccctc gtgggcctt tttatcgac aaagttggc 
aaaaatatt ttatggatc gttgtggca atttcagtg 
acgataggg tcacacacc ttgtgggcc tttaccgac 
gtcaacgcc tacgttgga atggttatc ttggggctt 
gcatacgca tgcttggcc agtagttta tggcctttg 
gtctctttg ataattccc gagtatcag ttaggaaca 
gcttacgga gtttgtcaa gctattcaa aatcttggc 
ctggccgtg tttacaata gtggctggg cacatcaaa 
gacacctac ggctacatc tggcttgaa gtgttcttc 
atttgcaca ctaatagtc gccttagtg ttcactctt 
gttttatgg ttggttgac atgtaccaa agaggtgtc 
ctgaacatg actcccgga aatcgggta gcgcatcaa 
gagaggcta tcaagcgcc gcagacgcg gagaaacaa 
agactctta tcgtctgaa gcgagttca agtaacgac 
aatgataat ttacaaccg agttcagat tttgaaatt 
aggaaccga tatttgtcg aggatcggg gcaccatta 
cctgatcac tattcaatt caaaacaaa ggactctct 
tacagagcg ttgagataa
    mRNA sequence
    • >TC014393 RA
      gtccaccaa ccaaacaac aaaaaagta tccaaacac 
tcaaaatta tgtcaataa aaaggaata aataaataa 
taaaaattg tgttatgcg ataatacca ttataatat 
caccatatc tcgctatga ttatgattt gtgcgaaac 
atttgagac aataaaccg cgacattat cactttcag 
aaggtcgtc cgtgtctcc caagtgaaa cacaaaaga 
ggttatttg gggggtgtc agcatgaat accagtgtt 
ggattaaat taaattgga ctacgagta agtgtgttt 
ctatttcat tgaacaacc ccacgaaaa tggagggtg 
gacccaaca tacaagaag cagacgaca caataaatt 
cctccactg ctgaggaag gaggaaatt cgggatgtt 
actacagct gctgtgacc ccaggggca ggctgcacc 
gctttatag ccctaattt tcatgtgct tcctcggtt 
ttggttcct atttctgtt acgacaacc ctgcagctt 
tagaaggcc acatcgaaa aaaccttaa atataacca 
caactcaat attccctac tttactcga tgtactctt 
ggccaaacg ttgtgctat gtttcgttg gaggctttc 
taattgacc gagttttcg gaattcgtc tcggcacca 
atatttatt cgggcctca cgttgttgg ggcaaatta 
ttttcgcca gcggggcgc tgtttgacg ttttttgga 
taatgatca tgggcagat ttatttttg gcatagggg 
ctgagtcgc tagcagtcg cacaaaata attacgccg 
tgttgtggt tcaaaggca aagaactta acatggttt 
ttgggctgc agttgagtt ttgcgcgga ttggtagca 
ctgtgaatt ttctcgtga tggaatcga tttaccagt 
gggttaata aggagtaca aagggacgg aattgctag 
gaattgttt tatttgtgg ccacttcga cctgtctgt 
tctccatgc tttgtgccc tagttttgg gttttatgg 
acaagcggg cggaaaatc tgttaagac gacacgaaa 
ccacaaacc ctgaagttg ttcgattta ccgacgtta 
ttgagttta aattcacct tttggctta ttgccgtga 
tctgtgttt cttattacg tcgctatat ttcccttta 
tcgcccttg gagaagacc tatttatta aaaaattcc 
acatgtcga aacaagagg cgaacacga ttaatagtc 
tcatttact tgatatcag gagtggctt cgcccctcg 
tgggccttt ttatcgaca aagttggca aaaatattt 
tatggatcg ttgtggcaa tttcagtga cgatagggt 
cacacacct tgtgggcct ttaccgacg tcaacgcct 
acgttggaa tggttatct tggggcttg catacgcat 
gcttggcca gtagtttat ggcctttgg tctctttga 
taattcccg agtatcagt taggaacag cttacggag 
tttgtcaag ctattcaaa atcttggcc tggccgtgt 
ttacaatag tggctgggc acatcaaag acacctacg 
gctacatct ggcttgaag tgttcttca tttgcacac 
taatagtcg ccttagtgt tcactcttg ttttatggt 
tggttgaca tgtaccaaa gaggtgtcc tgaacatga 
ctcccggaa atcgggtag cgcatcaag agaggctat 
caagcgccg cagacgcgg agaaacaaa gactcttat 
cgtctgaag cgagttcaa gtaacgaca atgataatt 
tacaaccga gttcagatt ttgaaatta ggaaccgat 
atttgtcga ggatcgggg caccagtaa gtgacaaca 
cggaagaat gaaaaggat gagaatagt taaatattt 
tttgtttca gttacctga tcactattc aattcaaaa 
caaaggact ctcttacag agcgttgag ataagtctt 
ttcaattat tatacgatt ttgtgatca aataaagtg 
ataaaacac aagactaat attgaatac aaaaaatat 
ttaacgatt ctgtaatga tcaaagcag aatttgaat 
caaagtgta ttgtgatac atatcgtaa gatactaag 
taaaaagtg taaaatatt aaaggttta gaacatttt 
ctactctct gtttccaca gttgtgtac tctatagat 
caatatatt tattgttag tacggaatt ttatagtgt 
aattgtaat taataaaga catttgtct att
    • >TC014393 RB
      gtccaccaa ccaaacaac aaaaaagta tccaaacac 
tcaaaatta tgtcaataa aaaggaata aataaataa 
taaaaattg tgttatgcg ataatacca ttataatat 
caccatatc tcgctatga ttatgattt gtgcgaaac 
atttgagac aataaaccg cgacattat cactttcag 
aaggtcgtc cgtgtctcc caagtgaaa cacaaaaga 
ggttatttg gggggtgtc agcatgaat accagtgtt 
ggattaaat taaattgga ctacgagta agtgtgttt 
ctatttcat tgaacaacc ccacgaaaa tggagggtg 
gacccaaca tacaagaag cagacgaca caataaatt 
cctccactg ctgaggaag gaggaaatt cgggatgtt 
actacagct gctgtgacc ccaggggca ggctgcacc 
gctttatag ccctaattt tcatgtgct tcctcggtt 
ttggttcct atttctgtt acgacaacc ctgcagctt 
tagaaggcc acatcgaaa aaaccttaa atataacca 
caactcaat attccctac tttactcga tgtactctt 
ggccaaacg ttgtgctat gtttcgttg gaggctttc 
taattgacc gagttttcg gaattcgtc tcggcacca 
atatttatt cgggcctca cgttgttgg ggcaaatta 
ttttcgcca gcggggcgc tgtttgacg ttttttgga 
taatgatca tgggcagat ttatttttg gcatagggg 
ctgagtcgc tagcagtcg cacaaaata attacgccg 
tgttgtggt tcaaaggca aagaactta acatggttt 
ttgggctgc agttgagtt ttgcgcgga ttggtagca 
ctgtgaatt ttctcgtga tggaatcga tttaccagt 
gggttaata aggagtaca aagggacgg aattgctag 
gaattgttt tatttgtgg ccacttcga cctgtctgt 
tctccatgc tttgtgccc tagttttgg gttttatgg 
acaagcggg cggaaaatc tgttaagac gacacgaaa 
ccacaaacc ctgaagttg ttcgattta ccgacgtta 
ttgagttta aattcacct tttggctta ttgccgtga 
tctgtgttt cttattacg tcgctatat ttcccttta 
tcgcccttg gagaagacc tatttatta aaaaattcc 
acatgtcga aacaagagg cgaacacga ttaatagtc 
tcatttact tgatatcag gagtggctt cgcccctcg 
tgggccttt ttatcgaca aagttggca aaaatattt 
tatggatcg ttgtggcaa tttcagtga cgatagggt 
cacacacct tgtgggcct ttaccgacg tcaacgcct 
acgttggaa tggttatct tggggcttg catacgcat 
gcttggcca gtagtttat ggcctttgg tctctttga 
taattcccg agtatcagt taggaacag cttacggag 
tttgtcaag ctattcaaa atcttggcc tggccgtgt 
ttacaatag tggctgggc acatcaaag acacctacg 
gctacatct ggcttgaag tgttcttca tttgcacac 
taatagtcg ccttagtgt tcactcttg ttttatggt 
tggttgaca tgtaccaaa gaggtgtcc tgaacatga 
ctcccggaa atcgggtag cgcatcaag agaggctat 
caagcgccg cagacgcgg agaaacaaa gactcttat 
cgtctgaag cgagttcaa gtaacgaca atgataatt 
tacaaccga gttcagatt ttgaaatta ggaaccgat 
atttgtcga ggatcgggg caccattac ctgatcact 
attcaattc aaaacaaag gactctctt acagagcgt 
tgagataag tcttttcaa ttattatac gattttgtg 
atcaaataa agtgataaa acacaagac taatattga 
atacaaaaa atatttaac gattctgta atgatcaaa 
gcagaattt gaatcaaag tgtattgtg atacatatc 
gtaagatac taagtaaaa agtgtaaaa tattaaagg 
tttagaaca ttttctact ctctgtttc cacagttgt 
gtactctat agatcaata tatttattg ttagtacgg 
aattttata gtgtaattg taattaata aagacattt 
gtctatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY