Status
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Gene information
General information
TC014383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9967853..9972130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014383
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05621

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014383 RA
      MDYLGKRER KVHPQEKAN ILSNLSFFY TLKLFRKGY 
      KNDLEEDDL YEVLKSYRS KELGDQLEA EWEKQKKQG 
      KNNSIVRLL WACYGWQYF LLGLVQLIV KTTMIVVQP 
      QALSKIISY FNPNQTEMT KKDAYFYAM LLIAINLIN 
      CIYVHNYQL AVTGLGIKV RTAFCSFIY RKALKLSPT 
      RLSDISIGR IVTLMTKDV HSFESFIHF ANDLWIGIV 
      KSGVIFYII YRKIGVSAF AGVLCFVIL FPMQAFLGS 
      KSAKLRMKM CKKTDDRLQ LTQETLSAI KIIKMYTWE 
      KFFDKKISD ARKKEIKTM HTNFYLKFI VLQIGNLSG 
      KMTFYLLIM TYTWLDNHV TAEIVYFVE SCLQIVTHT 
      ISILFPIGI TQTAELSAS IKRIGNVLK ALEVQTEQH 
      EDHLTIKPK ITLKDVSVN FKDKEILHS INLTLDMGV 
      TLITGPVGS GKSFLLKTI LQDYEPSSG GLVSKGRIS 
      YASQEPWLF PSSIKQNIL FGQKYDEKR YNEVLKVCA 
      LVYDFDLLS AGDNTIVED RGINLSKGQ QARINLARA 
      VYKESEIYL LDDCLSALD AHVSDFIFK ECILKFLRN 
      KLVVFVSHN ASHVKDVDN VVIMHDGSV SSCVKSSEI 
      SETQILEEI INDKEKTED LIDEDEDEA NEKTKLVTE 
      TTKERKVYQ EIKKKGEVD LSVYTKYIK FGGGFFIFT 
      LLLVAFGVS QFVHSYADK LVSQWVNLE RKISNFTFS 
      NSTNLTEQQ KGTIISHRD FTQHLFSAM TALTVAFEL 
      GRILALFAV ARRASIKLH KYMTDHIIK ATMHFFDTN 
      FIGNILNRF SKDLSTIDE HLPFVIFQV FRAFLVIVG 
      VLILIYTVI KSFLIPTLM FFCLLAVIR RYYLPTGRS 
      LKRLDAATR SPVVGHLNA TLEGLTTIR AFKAEQILR 
      DEYDRHQDL YTSATYNFQ CSMRAFAYC LDTFNTLFI 
      ASVVLRFVI FDDVKEAGD VGLAITQAF RMTGTLQWG 
      IRQWAEIEN SMTSVERVL EYTEVKQEN NQGQTLDCW 
      PTKGEVRYE NVYLSYTNS EEYVLKDIN FTANPREKI 
      GIVGRTGAG KSSIISTLF RLYEVKGTI TIDGVDIKT 
      LSLDYLRRN ISIIPQDPV LFSGRIRDN IDPEGLYTD 
      DQIWKAIET ANLKPLVPS LDYEITENG SNFSVGQRQ 
      LICLARAVI RNNRIIVLD EATANMDRE TDALIHQTI 
      HENFASCTV FTIAHKLHT IINSDKVIV MDKGQIIEC 
      DDPNSLLQN TEGVFYNMV KKSGLLPTA EA
    CDS sequence
    • >TC014383 RA
      atggactat ctcggaaaa cgtgaacgc aaagtccat 
      ccccaagag aaagccaac attttatca aacttatca 
      tttttctac actttaaag ctattcaga aagggttac 
      aaaaatgat ctggaagaa gacgacttg tacgaagtt 
      ttgaaaagt tacagatcg aaagaactg ggcgaccag 
      ttggaggca gaatgggaa aaacagaaa aagcaaggc 
      aaaaacaat tcaatagtc cgcttgttg tgggcttgc 
      tacgggtgg caatacttc ctccttggt ctagtgcag 
      ctcattgtc aaaacaacg atgatcgta gtccaaccg 
      caagctttg agcaaaatt atttcgtac ttcaacccg 
      aaccaaact gaaatgacc aaaaaggac gcgtatttc 
      tatgcgatg cttttgatc gcaatcaat ttaatcaat 
      tgcatctac gtccacaac tatcagcta gctgtaaca 
      ggactgggg atcaaagtt cgtacagct ttctgctcg 
      tttatttac agaaaagct ctaaagttg agtcctaca 
      agactcagc gacatatca atcggacgc attgtgacg 
      ctaatgacc aaagatgtg cactctttc gaaagcttc 
      attcacttc gctaacgac ttatggatc ggaattgtc 
      aagtccggg gttattttt tacataatt taccggaaa 
      attggggtt tctgccttt gcaggtgtc ttgtgtttt 
      gtcattttg ttcccaatg caagctttt ttgggttct 
      aaatcagcg aagctgcga atgaaaatg tgtaagaaa 
      acagacgat aggttacaa ctgactcag gaaacttta 
      tcggcgatc aaaatcatc aaaatgtat acttgggag 
      aagtttttt gacaagaaa atttcagat gccagaaag 
      aaagagatt aaaacaatg cacaccaac ttctacctg 
      aagtttatt gtgctacag atcggtaat ttgagtggc 
      aaaatgacc ttctacctc ctcatcatg acctacact 
      tggctcgat aaccacgta accgctgaa atcgtttat 
      tttgtcgaa agttgcctc caaatcgtc actcacacc 
      atcagcatt cttttcccc atcggaatc acacaaaca 
      gccgaactg tccgcttct attaaacga atcggcaat 
      gtgttaaaa gcattagaa gtgcaaacg gaacaacac 
      gaagatcat ttaacaatc aaaccaaaa atcactctt 
      aaggacgtc agtgttaac ttcaaggac aaggaaatc 
      ctccactcc ataaacctg acgcttgac atgggagtc 
      actctcatc actggtcct gtgggaagc ggcaaaagc 
      ttcctcttg aagacgatc ctccaggac tacgaacct 
      tcaagcgga ggcttagtt tccaaagga cgaatttcc 
      tacgcttcg caggaaccg tggctgttc ccgtcctca 
      atcaagcaa aatatctta ttcgggcaa aagtacgac 
      gaaaaacgc tacaacgaa gtcctgaag gtgtgtgcg 
      ctcgtgtac gactttgac ctgctatca gccggtgat 
      aacacaatt gttgaagac cgcggaatc aatttgagc 
      aaaggacaa caagctcgg atcaaccta gctcgggct 
      gtgtacaaa gagagcgag atttacctc ctggacgat 
      tgcctgtcg gcgttggac gcccacgtc agtgatttt 
      attttcaaa gagtgcatt ttgaaattc ttgagaaac 
      aaactggtg gtcttcgtc agccacaac gccagtcat 
      gtgaaggac gttgacaat gttgttatc atgcacgat 
      ggctcggtc tcgtcctgc gtcaagtcg tccgaaatt 
      tccgaaacc caaattttg gaggagata atcaacgat 
      aaggaaaaa actgaggat ttaatcgat gaggacgag 
      gacgaagcg aacgaaaaa accaaacta gtgaccgaa 
      accaccaaa gagcggaag gtttaccag gaaattaaa 
      aagaaaggc gaggtggac ctctccgtt tacaccaaa 
      tacatcaaa tttggtggc gggtttttc atcttcacg 
      ctactgcta gtcgcgttt ggcgtttcg caatttgtt 
      cacagctat gcggacaaa ttggttagc caatgggtt 
      aatctcgag aggaaaatc tccaacttc acttttagt 
      aatagtacg aatctgacg gaacaacag aaggggacc 
      ataataagc catcgcgat ttcacccaa cacctgttc 
      agcgcaatg acggctcta actgtagct ttcgaactt 
      ggccgaatt ttggcactt tttgccgtt gcaaggaga 
      gcctccatc aagctgcac aaatatatg actgaccat 
      attatcaaa gccacgatg catttcttt gacaccaat 
      tttatcggc aatattttg aaccggttc tcgaaggat 
      ttgtcgacg attgacgag catttgccg tttgtgatt 
      ttccaagtc tttagagca tttctcgta atagtcggc 
      gttttgatc ctgatatac acagtcatt aaatcattt 
      ctaatcccg acactgatg ttcttctgc cttttggca 
      gtaattagg cgatattat ctgccaaca ggacgaagt 
      cttaagcgt ctggatgct gctactcga agtccagtc 
      gtgggtcac ttgaacgca acccttgaa ggtctgaca 
      acaatccgg gctttcaaa gccgaacaa atcctgcga 
      gatgaatac gacaggcac caggacctt tacacttca 
      gccacgtac aatttccag tgcagcatg cgggctttt 
      gcctactgt ttggacact ttcaacaca ctttttatc 
      gcatcggtg gttttaaga tttgttatt ttcgatgac 
      gtcaaagag gccggagac gtaggtctt gccatcact 
      caggcgttc cgaatgacg ggaactttg cagtggggg 
      atccggcag tgggccgaa attgaaaat tcgatgact 
      tcagtcgaa cgcgtcctt gaatacacc gaagtcaag 
      caggagaac aatcaagga cagacgctg gactgttgg 
      cccacgaaa ggcgaagtc agatacgaa aacgtctac 
      ttgtcgtac accaactcc gaggagtat gtcctgaaa 
      gacattaat tttaccgcg aacccgagg gaaaaaatc 
      gggatcgtc ggcagaaca ggcgctggg aagtcgtca 
      atcatctcg actttgttc cggttgtac gaagtcaaa 
      ggaacaatc acgattgac ggagttgac atcaaaacc 
      ttgtctttg gattacttg cggaggaat atttccatc 
      atcccgcaa gacccagtt cttttcagt gggagaatc 
      cgcgataat attgatcct gaaggactt tacacagat 
      gatcaaatt tggaaagct atcgaaacg gccaatttg 
      aagccgctt gttccgagt ttagattac gaaattacg 
      gaaaatggt tccaacttt agcgttggc cagagacag 
      ttgatttgc ttggcgaga gctgtcatc cgtaataac 
      agaattatc gtcctggat gaagctaca gccaatatg 
      gacagagag accgatgct ttgattcac caaacaatt 
      catgagaac tttgcgtct tgtactgtt ttcacaata 
      gcgcacaaa ttacacacg attataaac tcggacaag 
      gtgattgtt atggataaa ggacagatt atcgagtgc 
      gatgatccc aatagtttg ttgcaaaat acagaaggt 
      gtgttttat aatatggtc aaaaaatcg gggctgcta 
      ccgacagcc gaagcataa
    mRNA sequence
    • >TC014383 RA
      tgtgagtgg cacctgaag accatggac tatctcgga 
      aaacgtgaa cgcaaagtc catccccaa gagaaagcc 
      aacatttta tcaaactta tcatttttc tacacttta 
      aagctattc agaaagggt tacaaaaat gatctggaa 
      gaagacgac ttgtacgaa gttttgaaa agttacaga 
      tcgaaagaa ctgggcgac cagttggag gcagaatgg 
      gaaaaacag aaaaagcaa ggcaaaaac aattcaata 
      gtccgcttg ttgtgggct tgctacggg tggcaatac 
      ttcctcctt ggtctagtg cagctcatt gtcaaaaca 
      acgatgatc gtagtccaa ccgcaagct ttgagcaaa 
      attatttcg tacttcaac ccgaaccaa actgaaatg 
      accaaaaag gacgcgtat ttctatgcg atgcttttg 
      atcgcaatc aatttaatc aattgcatc tacgtccac 
      aactatcag ctagctgta acaggactg gggatcaaa 
      gttcgtaca gctttctgc tcgtttatt tacagaaaa 
      gctctaaag ttgagtcct acaagactc agcgacata 
      tcaatcgga cgcattgtg acgctaatg accaaagat 
      gtgcactct ttcgaaagc ttcattcac ttcgctaac 
      gacttatgg atcggaatt gtcaagtcc ggggttatt 
      ttttacata atttaccgg aaaattggg gtttctgcc 
      tttgcaggt gtcttgtgt tttgtcatt ttgttccca 
      atgcaagct tttttgggt tctaaatca gcgaagctg 
      cgaatgaaa atgtgtaag aaaacagac gataggtta 
      caactgact caggaaact ttatcggcg atcaaaatc 
      atcaaaatg tatacttgg gagaagttt tttgacaag 
      aaaatttca gatgccaga aagaaagag attaaaaca 
      atgcacacc aacttctac ctgaagttt attgtgcta 
      cagatcggt aatttgagt ggcaaaatg accttctac 
      ctcctcatc atgacctac acttggctc gataaccac 
      gtaaccgct gaaatcgtt tattttgtc gaaagttgc 
      ctccaaatc gtcactcac accatcagc attcttttc 
      cccatcgga atcacacaa acagccgaa ctgtccgct 
      tctattaaa cgaatcggc aatgtgtta aaagcatta 
      gaagtgcaa acggaacaa cacgaagat catttaaca 
      atcaaacca aaaatcact cttaaggac gtcagtgtt 
      aacttcaag gacaaggaa atcctccac tccataaac 
      ctgacgctt gacatggga gtcactctc atcactggt 
      cctgtggga agcggcaaa agcttcctc ttgaagacg 
      atcctccag gactacgaa ccttcaagc ggaggctta 
      gtttccaaa ggacgaatt tcctacgct tcgcaggaa 
      ccgtggctg ttcccgtcc tcaatcaag caaaatatc 
      ttattcggg caaaagtac gacgaaaaa cgctacaac 
      gaagtcctg aaggtgtgt gcgctcgtg tacgacttt 
      gacctgcta tcagccggt gataacaca attgttgaa 
      gaccgcgga atcaatttg agcaaagga caacaagct 
      cggatcaac ctagctcgg gctgtgtac aaagagagc 
      gagatttac ctcctggac gattgcctg tcggcgttg 
      gacgcccac gtcagtgat tttattttc aaagagtgc 
      attttgaaa ttcttgaga aacaaactg gtggtcttc 
      gtcagccac aacgccagt catgtgaag gacgttgac 
      aatgttgtt atcatgcac gatggctcg gtctcgtcc 
      tgcgtcaag tcgtccgaa atttccgaa acccaaatt 
      ttggaggag ataatcaac gataaggaa aaaactgag 
      gatttaatc gatgaggac gaggacgaa gcgaacgaa 
      aaaaccaaa ctagtgacc gaaaccacc aaagagcgg 
      aaggtttac caggaaatt aaaaagaaa ggcgaggtg 
      gacctctcc gtttacacc aaatacatc aaatttggt 
      ggcgggttt ttcatcttc acgctactg ctagtcgcg 
      tttggcgtt tcgcaattt gttcacagc tatgcggac 
      aaattggtt agccaatgg gttaatctc gagaggaaa 
      atctccaac ttcactttt agtaatagt acgaatctg 
      acggaacaa cagaagggg accataata agccatcgc 
      gatttcacc caacacctg ttcagcgca atgacggct 
      ctaactgta gctttcgaa cttggccga attttggca 
      ctttttgcc gttgcaagg agagcctcc atcaagctg 
      cacaaatat atgactgac catattatc aaagccacg 
      atgcatttc tttgacacc aattttatc ggcaatatt 
      ttgaaccgg ttctcgaag gatttgtcg acgattgac 
      gagcatttg ccgtttgtg attttccaa gtctttaga 
      gcatttctc gtaatagtc ggcgttttg atcctgata 
      tacacagtc attaaatca tttctaatc ccgacactg 
      atgttcttc tgccttttg gcagtaatt aggcgatat 
      tatctgcca acaggacga agtcttaag cgtctggat 
      gctgctact cgaagtcca gtcgtgggt cacttgaac 
      gcaaccctt gaaggtctg acaacaatc cgggctttc 
      aaagccgaa caaatcctg cgagatgaa tacgacagg 
      caccaggac ctttacact tcagccacg tacaatttc 
      cagtgcagc atgcgggct tttgcctac tgtttggac 
      actttcaac acacttttt atcgcatcg gtggtttta 
      agatttgtt attttcgat gacgtcaaa gaggccgga 
      gacgtaggt cttgccatc actcaggcg ttccgaatg 
      acgggaact ttgcagtgg gggatccgg cagtgggcc 
      gaaattgaa aattcgatg acttcagtc gaacgcgtc 
      cttgaatac accgaagtc aagcaggag aacaatcaa 
      ggacagacg ctggactgt tggcccacg aaaggcgaa 
      gtcagatac gaaaacgtc tacttgtcg tacaccaac 
      tccgaggag tatgtcctg aaagacatt aattttacc 
      gcgaacccg agggaaaaa atcgggatc gtcggcaga 
      acaggcgct gggaagtcg tcaatcatc tcgactttg 
      ttccggttg tacgaagtc aaaggaaca atcacgatt 
      gacggagtt gacatcaaa accttgtct ttggattac 
      ttgcggagg aatatttcc atcatcccg caagaccca 
      gttcttttc agtgggaga atccgcgat aatattgat 
      cctgaagga ctttacaca gatgatcaa atttggaaa 
      gctatcgaa acggccaat ttgaagccg cttgttccg 
      agtttagat tacgaaatt acggaaaat ggttccaac 
      tttagcgtt ggccagaga cagttgatt tgcttggcg 
      agagctgtc atccgtaat aacagaatt atcgtcctg 
      gatgaagct acagccaat atggacaga gagaccgat 
      gctttgatt caccaaaca attcatgag aactttgcg 
      tcttgtact gttttcaca atagcgcac aaattacac 
      acgattata aactcggac aaggtgatt gttatggat 
      aaaggacag attatcgag tgcgatgat cccaatagt 
      ttgttgcaa aatacagaa ggtgtgttt tataatatg 
      gtcaaaaaa tcggggctg ctaccgaca gccgaagca 
      taaagtgat ttgtattta tttaagggt gtttattta 
      tgttaattt gtggttcct aaaaatatg tgttgtttt 
      cttaaatta agaaaaagc aataataat aataataat 
      aaaaaatac agaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY