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Gene information
General information
TC014379 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9949123..9953338
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014379
    iBeetle screen iB_05619

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014379 RA
      MYHGVDQKK RTNPKEKAN LFSLITFFF THDLLKKGY 
      KNDFVEEDV YEVLHKFRS SKLGDTLEE QWAKEQKRK 
      NPSVYRLLW ACYGKYYLF LGFIQLGMK ICVLAIQPN 
      ALGKFVSYF TSSTKLTRA DAIYYACVL IAINIINTT 
      YGHNYMFHI MELGVRIKT AFCSLIYRK SLKLTSSSL 
      SATSVGKIV TLVTKDVTN FENAIIFMN DMWISLILL 
      VLTSWMMYK KIGVAALAG VLFLVLIIP LQVLFGKLT 
      TTLRLKASK VTDQRIQLT QEILSAIKT IKMYTWERF 
      FGEKISKAR EKEVTKVKQ IFFSKATSL VIGGLVISV 
      TFYIIVITY IQMGNYFTA EVAVFLNGC LHILRSSVT 
      ISIPIGIAQ TAELIASVN RIRKFLTAD EQPQTSTES 
      DSKPIIELK QVAVEVNKI PILHNVNLT FKSGLNFIT 
      GKLGGGKSS LLKTILRDY PTCAGSLTI HGTVSYASE 
      EPWLFPSSI RQNILFGQK YNPERYNKV LQVCALEQD 
      FDTFEKGDS TIVDDRGMN LSKGQQARI SLARAVYCD 
      SDIYLLDDC LSSLDTQVQ EYVFRECVK TFLKDKIVV 
      MISHNERQI KDGDCVVIM EGGTVKSCL KPSEIRDEE 
      IEELVREDD KGVEDDIID NIVLENDES REDTKLIDK 
      PKHNIYSEQ KKEGKVDFG VYRKYFTHG GSLFVLFLI 
      ILLFVSAQS ASIFSENCV KNWINLQQN ISDYKFNAS 
      NISETEYHT LIRKNTKVI NMYTAAIIT STVLLLLRA 
      FALFAFATR ASVALHKAM ALSVVNATM KFFDLHYIG 
      NILNRFSKD LAMIDEILP HTLYECFRM ICFISGVLI 
      LILIVRALF IIPICIMAA LLFILRKYY LPTGRSLKR 
      LESATRSPF IGHLNASLE GLTIIRAYR TEDILREEF 
      DKHQDLYTS TFYMMQCTA RAFAYFADM ICSLFMAII 
      ITWLLITRS DSLPGNVTL AITQALGLT GLLQWAIRQ 
      WAELENYMT SVERVLEYA NVETENRAG QVLDDWPTG 
      GKITYQRVN LTYGKSKEF VLKDITFAI NPREKIGIV 
      GRTGAGKSS LISTLFRLY RCEGAIAID DVDIKTLSL 
      DFLRSNIAI IPQDPVLFS GTIRSNIDP IGLHTDEEI 
      WKAVTTANL KTLVTSLDY EITESGSNY SSGQRQLIC 
      LARAIISKC KIIILDEAT ANVDPETDV MIYSIIQEN 
      FASCTVLTI AHRLHTVMY SDRVMVVDK GEIVEFDSP 
      SVLLSNQDG FFYKMIQQA NLLK
    CDS sequence
    • >TC014379 RA
      atgtatcat ggtgtggac cagaaaaag cgaacaaac 
      cccaaagaa aaagcaaat ttgttctca ctgatcact 
      tttttcttc acgcacgac ttgctcaaa aagggctac 
      aaaaatgat ttcgtcgaa gaagacgtt tacgaggtt 
      ttgcataaa tttagatca tcgaaactg ggagatacg 
      ttagaagaa cagtgggcg aaggaacag aaacggaag 
      aacccgtca gtgtatcgg ctcttatgg gcctgttat 
      ggcaaatat tatctcttt ctgggcttc atccagtta 
      gggatgaag atttgtgtt ttagcgatt caaccaaat 
      gctttaggg aagttcgtc tcgtatttc acttcgtcc 
      accaaactt acaagagcg gatgccatt tattacgct 
      tgtgttttg atcgcgata aacatcatt aacaccacc 
      tatggccac aattacatg ttccatatt atggagcta 
      ggagtccgt atcaagacg gctttctgc tccctcatc 
      taccgcaag tctttaaaa ttaacttct tcgtcattg 
      tctgcgaca agtgtggga aaaatcgta acacttgtc 
      acaaaagac gtgacaaat ttcgaaaat gcgataatt 
      ttcatgaac gacatgtgg atcagtttg atactcctt 
      gtgttgaca tcctggatg atgtataag aaaatcgga 
      gtggcggct ttggccgga gttctcttc ctagtgctg 
      ataataccc ttgcaagtt ctgtttggg aaactgacc 
      acgaccctg agactcaaa gccagcaaa gtgaccgat 
      cagcggatc caactgact caagagatc ctctccgcc 
      ataaaaacc atcaaaatg tacacttgg gagcgcttt 
      ttcggcgag aaaatctcc aaagcccgc gaaaaggaa 
      gtgacaaaa gtcaagcaa atttttttc tccaaagcc 
      acgtccctg gtcatcggt ggcttggtc atcagcgtc 
      actttctac atcatcgtg ataacgtac atccaaatg 
      gggaactac ttcacggcc gaagtggcc gtgttcttg 
      aacggctgt ttgcacata ttacgctcc tcagtcacg 
      atttccatc ccaatagga atcgcccaa acggccgaa 
      ctgatcgct tctgtcaac cgaattcgc aaatttctc 
      acagctgac gagcaaccg caaacgagc accgagtca 
      gactcaaaa cccataatc gaattaaaa caggtcgct 
      gttgaagtc aacaaaatc ccgatcttg cacaatgtt 
      aacctgacg ttcaagtcc ggcttgaat tttattacc 
      ggcaagctc ggaggtggc aaaagctcg ctcttgaag 
      accatcctc cgggattac cccacttgt gccgggagt 
      ctgaccatt cacggcacc gtgtcgtat gcctcggag 
      gaaccgtgg ctgttcccg tcgtccatc cgccaaaac 
      attctattc ggccagaaa tacaaccca gagcggtac 
      aataaagtc ctccaagtg tgtgccttg gagcaagac 
      tttgacact ttcgaaaag ggggacagt accatcgtg 
      gacgaccgc ggaatgaat ttgagcaaa ggccagcaa 
      gccaggatc agtctggcc agagctgtg tactgcgac 
      agcgatatt tacttacta gacgactgt ttgtcgtct 
      ctggatact caagtccag gagtacgtc ttcagggag 
      tgtgtcaag acgttcctc aaggacaaa atcgttgtt 
      atgatttcg cacaacgag cgccaaatc aaagacggt 
      gattgtgtg gtcattatg gaaggtggt accgtaaaa 
      tcgtgtttg aaaccgtcc gaaatacgc gacgaagaa 
      atcgaagaa ctggtccgt gaagacgac aaaggtgtc 
      gaagacgat attatcgac aacatcgtg ctggaaaac 
      gacgagtca cgcgaagac accaaacta atagacaaa 
      ccgaaacac aacatctac agtgagcag aagaaagag 
      gggaaggtc gattttgga gtttaccga aagtacttc 
      acacatggc ggctcactg ttcgttttg tttttgata 
      atcctactg tttgtttcg gcccaaagt gcgagtatt 
      ttttcagag aactgtgtc aagaactgg attaacttg 
      caacaaaac atatcggac tacaagttc aacgcttcc 
      aacatttcc gaaaccgaa tatcacacc ctgattagg 
      aaaaacacc aaagttatc aacatgtat accgctgct 
      attatcact tccacagtg ctgttgctc ttaagggcg 
      ttcgccctt tttgcgttt gccacgcgg gcctcagtt 
      gcccttcac aaagcaatg gccttgtcg gtggtcaac 
      gccacaatg aaatttttc gacttgcac tacatcggg 
      aatattttg aaccgcttt tccaaagac cttgcaatg 
      attgacgag attttaccc cacactctc tatgaatgc 
      tttcggatg atttgcttc atcagtggc gtcttaatt 
      ttgattcta atagtcaga gctttgttc atcatcccc 
      atttgtatc atggctgct ttacttttc atcctgagg 
      aaatattat ttgccaact gggagaagt ctcaaaaga 
      ctggaatca gcaacacgt agtccattc attgggcat 
      ctgaatgcc tctttggaa ggcctcact ataattagg 
      gcctaccgc actgaggac attctgcga gaagagttc 
      gacaaacac caggacctt tacacttca accttctac 
      atgatgcag tgcacagca cgagcattt gcctatttc 
      gcagacatg atctgtagc ctcttcatg gccattata 
      ataacctgg ctcttaatt acgagatca gattccctg 
      cctgggaac gtcactttg gccataacg caggccctt 
      ggcttgacc ggcctgctc cagtgggcg ataagacag 
      tgggcagag ctagagaac tacatgact tcagtggag 
      agagtccta gaatacgca aacgtggag acggaaaac 
      cgagctggc caggtgttg gacgactgg cccacaggt 
      ggcaaaatc acctaccag agggttaac ttaacgtac 
      ggcaagtcg aaagaattt gtcttgaaa gacatcact 
      tttgcgata aatccgcgc gagaaaatc ggcattgtt 
      ggacgcaca ggtgctggg aaaagctcg ctgatttcg 
      actctcttc cgcttgtac cgatgcgaa ggggcaatt 
      gccatagac gacgtcgat attaaaacc ctctctttg 
      gattttctg cggtcgaac atcgccatt atccctcag 
      gatcccgtt ttgttcagc gggacaatc cgcagcaat 
      attgatcca atcggcctc cacacggac gaggagatt 
      tggaaggcg gtcacgacc gcgaatttg aagactttg 
      gttacaagt ctggactat gaaataacc gaaagtggg 
      tccaattac agctctggg caaaggcaa ctgatttgc 
      ttggcgcgg gcgatcatc agcaagtgc aaaattatc 
      attctggat gaagcgaca gcgaacgtg gatcccgag 
      acggatgtg atgatctac tcgattatc caggagaat 
      ttcgcgtct tgcacggtt ttgaccatc gcgcataga 
      ctgcatact gttatgtat tccgacaga gtaatggtt 
      gtggataag ggagaaatc gtggagttt gatagtccc 
      agtgttttg cttagcaat caagacgga tttttctac 
      aagatgatt cagcaagcc aacttgttg aagtaa
    mRNA sequence
    • >TC014379 RA
      ttcctatta tttattcta gattagata tattggatc 
      aggtgtgaa ccacacatg gtggtacat cagtgaccc 
      tctaaaatg tatcatggt gtggaccag aaaaagcga 
      acaaacccc aaagaaaaa gcaaatttg ttctcactg 
      atcactttt ttcttcacg cacgacttg ctcaaaaag 
      ggctacaaa aatgatttc gtcgaagaa gacgtttac 
      gaggttttg cataaattt agatcatcg aaactggga 
      gatacgtta gaagaacag tgggcgaag gaacagaaa 
      cggaagaac ccgtcagtg tatcggctc ttatgggcc 
      tgttatggc aaatattat ctctttctg ggcttcatc 
      cagttaggg atgaagatt tgtgtttta gcgattcaa 
      ccaaatgct ttagggaag ttcgtctcg tatttcact 
      tcgtccacc aaacttaca agagcggat gccatttat 
      tacgcttgt gttttgatc gcgataaac atcattaac 
      accacctat ggccacaat tacatgttc catattatg 
      gagctagga gtccgtatc aagacggct ttctgctcc 
      ctcatctac cgcaagtct ttaaaatta acttcttcg 
      tcattgtct gcgacaagt gtgggaaaa atcgtaaca 
      cttgtcaca aaagacgtg acaaatttc gaaaatgcg 
      ataattttc atgaacgac atgtggatc agtttgata 
      ctccttgtg ttgacatcc tggatgatg tataagaaa 
      atcggagtg gcggctttg gccggagtt ctcttccta 
      gtgctgata atacccttg caagttctg tttgggaaa 
      ctgaccacg accctgaga ctcaaagcc agcaaagtg 
      accgatcag cggatccaa ctgactcaa gagatcctc 
      tccgccata aaaaccatc aaaatgtac acttgggag 
      cgctttttc ggcgagaaa atctccaaa gcccgcgaa 
      aaggaagtg acaaaagtc aagcaaatt tttttctcc 
      aaagccacg tccctggtc atcggtggc ttggtcatc 
      agcgtcact ttctacatc atcgtgata acgtacatc 
      caaatgggg aactacttc acggccgaa gtggccgtg 
      ttcttgaac ggctgtttg cacatatta cgctcctca 
      gtcacgatt tccatccca ataggaatc gcccaaacg 
      gccgaactg atcgcttct gtcaaccga attcgcaaa 
      tttctcaca gctgacgag caaccgcaa acgagcacc 
      gagtcagac tcaaaaccc ataatcgaa ttaaaacag 
      gtcgctgtt gaagtcaac aaaatcccg atcttgcac 
      aatgttaac ctgacgttc aagtccggc ttgaatttt 
      attaccggc aagctcgga ggtggcaaa agctcgctc 
      ttgaagacc atcctccgg gattacccc acttgtgcc 
      gggagtctg accattcac ggcaccgtg tcgtatgcc 
      tcggaggaa ccgtggctg ttcccgtcg tccatccgc 
      caaaacatt ctattcggc cagaaatac aacccagag 
      cggtacaat aaagtcctc caagtgtgt gccttggag 
      caagacttt gacactttc gaaaagggg gacagtacc 
      atcgtggac gaccgcgga atgaatttg agcaaaggc 
      cagcaagcc aggatcagt ctggccaga gctgtgtac 
      tgcgacagc gatatttac ttactagac gactgtttg 
      tcgtctctg gatactcaa gtccaggag tacgtcttc 
      agggagtgt gtcaagacg ttcctcaag gacaaaatc 
      gttgttatg atttcgcac aacgagcgc caaatcaaa 
      gacggtgat tgtgtggtc attatggaa ggtggtacc 
      gtaaaatcg tgtttgaaa ccgtccgaa atacgcgac 
      gaagaaatc gaagaactg gtccgtgaa gacgacaaa 
      ggtgtcgaa gacgatatt atcgacaac atcgtgctg 
      gaaaacgac gagtcacgc gaagacacc aaactaata 
      gacaaaccg aaacacaac atctacagt gagcagaag 
      aaagagggg aaggtcgat tttggagtt taccgaaag 
      tacttcaca catggcggc tcactgttc gttttgttt 
      ttgataatc ctactgttt gtttcggcc caaagtgcg 
      agtattttt tcagagaac tgtgtcaag aactggatt 
      aacttgcaa caaaacata tcggactac aagttcaac 
      gcttccaac atttccgaa accgaatat cacaccctg 
      attaggaaa aacaccaaa gttatcaac atgtatacc 
      gctgctatt atcacttcc acagtgctg ttgctctta 
      agggcgttc gcccttttt gcgtttgcc acgcgggcc 
      tcagttgcc cttcacaaa gcaatggcc ttgtcggtg 
      gtcaacgcc acaatgaaa tttttcgac ttgcactac 
      atcgggaat attttgaac cgcttttcc aaagacctt 
      gcaatgatt gacgagatt ttaccccac actctctat 
      gaatgcttt cggatgatt tgcttcatc agtggcgtc 
      ttaattttg attctaata gtcagagct ttgttcatc 
      atccccatt tgtatcatg gctgcttta cttttcatc 
      ctgaggaaa tattatttg ccaactggg agaagtctc 
      aaaagactg gaatcagca acacgtagt ccattcatt 
      gggcatctg aatgcctct ttggaaggc ctcactata 
      attagggcc taccgcact gaggacatt ctgcgagaa 
      gagttcgac aaacaccag gacctttac acttcaacc 
      ttctacatg atgcagtgc acagcacga gcatttgcc 
      tatttcgca gacatgatc tgtagcctc ttcatggcc 
      attataata acctggctc ttaattacg agatcagat 
      tccctgcct gggaacgtc actttggcc ataacgcag 
      gcccttggc ttgaccggc ctgctccag tgggcgata 
      agacagtgg gcagagcta gagaactac atgacttca 
      gtggagaga gtcctagaa tacgcaaac gtggagacg 
      gaaaaccga gctggccag gtgttggac gactggccc 
      acaggtggc aaaatcacc taccagagg gttaactta 
      acgtacggc aagtcgaaa gaatttgtc ttgaaagac 
      atcactttt gcgataaat ccgcgcgag aaaatcggc 
      attgttgga cgcacaggt gctgggaaa agctcgctg 
      atttcgact ctcttccgc ttgtaccga tgcgaaggg 
      gcaattgcc atagacgac gtcgatatt aaaaccctc 
      tctttggat tttctgcgg tcgaacatc gccattatc 
      cctcaggat cccgttttg ttcagcggg acaatccgc 
      agcaatatt gatccaatc ggcctccac acggacgag 
      gagatttgg aaggcggtc acgaccgcg aatttgaag 
      actttggtt acaagtctg gactatgaa ataaccgaa 
      agtgggtcc aattacagc tctgggcaa aggcaactg 
      atttgcttg gcgcgggcg atcatcagc aagtgcaaa 
      attatcatt ctggatgaa gcgacagcg aacgtggat 
      cccgagacg gatgtgatg atctactcg attatccag 
      gagaatttc gcgtcttgc acggttttg accatcgcg 
      catagactg catactgtt atgtattcc gacagagta 
      atggttgtg gataaggga gaaatcgtg gagtttgat 
      agtcccagt gttttgctt agcaatcaa gacggattt 
      ttctacaag atgattcag caagccaac ttgttgaag 
      taattttag gtttatggt ttttcgtaa atgtgattt 
      ctgagcagt tttgtactt agcagtaat aaagaattt 
      aaagtaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY