Status
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Gene information
General information
TC033147
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014366
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9810553..9816775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033147
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05617

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033147 RA
      MVDAELCIA LAFSLQYVR LCVLCWMLT MAHHMHSQF 
      RTSLHLNPI TDNAIGKHF CRYSVFAWG VPGILLGAS 
      IMVQYHDKA GKLLDTASL KSQNCWFLD RNAFIYGLL 
      VPSTIFISV TLYYLVRSA LVARYVISM QVDRKLRDK 
      MRRKRMLHI ALFAKITVL LGLVVTLGA LSKLARSET 
      AWIAYHVGQ GLQGIFVAM LVTCNCQVL KLYTRSMKS 
      RASRHIPVY ASMGRASST AVSKSTSLQ LLTWEPAPD 
      PV
    CDS sequence
    • >TC033147 RA
      atggtggat gccgagctt tgcatagct ttggccttc 
      tcgctacaa tacgttcgg ctctgcgtc ctgtgctgg 
      atgctgacg atggcacat cacatgcat tcacagttt 
      aggactagt ctgcattta aatccgatc acggacaat 
      gcaattggg aagcatttt tgtagatat tcagtgttc 
      gcctggggt gttcctgga atactcctc ggcgccagc 
      attatggta caataccac gataaagcg ggaaaactg 
      ctggatact gcaagtctc aagtcgcag aattgctgg 
      tttcttgat cggaatgct ttcatatat ggtttattg 
      gttccttcc accatcttc atatcagtc accttatac 
      tatttggtg agatcggct ttagtagca cgctatgtt 
      atcagtatg caagtagat cgcaagcta cgtgataaa 
      atgcgacgt aagaggatg ctgcacatt gctcttttt 
      gccaagatt acagtcttg ctcggctta gtggtaact 
      ctgggcgcc ctcagcaag ctagctagg tccgaaacg 
      gcttggatc gcttaccac gtaggacaa ggtcttcaa 
      ggcattttc gtcgccatg ctggtgaca tgcaattgc 
      caagtgcta aagctctac actcgaagt atgaaatcg 
      agagcttcg cgtcatata ccagtctac gcaagcatg 
      ggtcgtgcc tcctccact gctgtttcc aaatctaca 
      agtctccag cttttgacg tgggaacca gcccctgat 
      ccagtgtaa
    mRNA sequence
    • >TC033147 RA
      gtgggggtg aggacgcat ccatcaatt tgacggctg 
      aattataat cctgacctg cattttgaa atattacac 
      cgagatggt ggatgccga gctttgcat agctttggc 
      cttctcgct acaatacgt tcggctctg cgtcctgtg 
      ctggatgct gacgatggc acatcacat gcattcaca 
      gtttaggac tagtctgca tttaaatcc gatcacgga 
      caatgcaat tgggaagca tttttgtag atattcagt 
      gttcgcctg gggtgttcc tggaatact cctcggcgc 
      cagcattat ggtacaata ccacgataa agcgggaaa 
      actgctgga tactgcaag tctcaagtc gcagaattg 
      ctggtttct tgatcggaa tgctttcat atatggttt 
      attggttcc ttccaccat cttcatatc agtcacctt 
      atactattt ggtgagatc ggctttagt agcacgcta 
      tgttatcag tatgcaagt agatcgcaa gctacgtga 
      taaaatgcg acgtaagag gatgctgca cattgctct 
      ttttgccaa gattacagt cttgctcgg cttagtggt 
      aactctggg cgccctcag caagctagc taggtccga 
      aacggcttg gatcgctta ccacgtagg acaaggtct 
      tcaaggcat tttcgtcgc catgctggt gacatgcaa 
      ttgccaagt gctaaagct ctacactcg aagtatgaa 
      atcgagagc ttcgcgtca tataccagt ctacgcaag 
      catgggtcg tgcctcctc cactgctgt ttccaaatc 
      tacaagtct ccagctttt gacgtggga accagcccc 
      tgatccagt gtaaaatgt atttttatc aaagcatta 
      ttgttgaaa aatatatca gaaagttct gtgtggcga 
      catcgaatt ttggaaatg tatcatttt gaaacacat 
      atcacgagc gtgttttgt aataatgat actgaatta 
      ttttcctaa atttctcaa aagtagaaa attgccagt 
      gggtgctaa aatgaaggc gagtgcttg caagctttt 
      gaaatcctg tcagtaaat aataattta gttaaggtg 
      atgcgatgg tgattatca tcaaagctt agtaaagat 
      tgttttatt ttattggga aaagccata actccactg 
      aaacaatta ttatttgta ttcttcatc ttgtatttt 
      tataatatg tttatctta ttcaaaaga aaatacaca 
      ttaaaacaa tagaaaaaa aatagtacc aaagagaca 
      tcttttctt ttcttatcg cattagatt agtttccag 
      aatgttaaa aaaacacac gactctaag acttggtag 
      tatttgtaa aacgccctg taaatactt ggttttgag 
      gtttatcca ataaagact agcatttta cgactgagt 
      ctttttata cgttactaa tgatttttt tactaattc 
      aaatgcata cacagatac tttgttgaa gtaaagaaa 
      gaatcgaaa caaccgcac tcatagtgg tcatagttt 
      tatttagca gaataaaat cgtaaaata acgcgtcac 
      gggacatct ttcttggta aggcaaata gtgtggctt 
      tggtggtct gatactgca agcttttat tgactcatc 
      aagaagata agtctaact ttttcaata ccaaagatc 
      gattcgaac aaacatcac tcgaacata tccataggt 
      cggtcctcg aggctgtgg gaactttaa tgtggttgt 
      ttcgtccac ttgacctca taacactca attcatgaa 
      cacatgttt caactccaa atcagattt tcgcttgac 
      aataaaatc acatatcta tgatgaagg gacagaaat 
      ctcgtttgc tctaataat agagtgacc ggttttcca 
      tctcttcac aataagttt ttgtattga gtcgagtta 
      ttaaattat ttccgtcac acactgaat tttctacat 
      atttgtata gaagtttat ttgagatcg tgtattttt 
      gtaatgact taatcttag ccgagtctc tattgtgta 
      cgctaggtt taggtaatc tgcagtaga caatatatc 
      aatgtaaat atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY