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Gene information
General information
TC014361 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9728470..9730134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014361
    iBeetle screen iB_05616

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014361 RA
      MNNTTVFEL LCTFITISL LSWYLYYGY CFNYWKKKG 
      VPTTPPKFP AGNILKPVL GKENLFVTV EDYYREFKR 
      EGHKHAGLY FFNGPVYLP IDPEIVKSI LTTDFDHFV 
      DRGVFYDEQ NFPLSTHLF SMRGTEWKS LRMKLSPTF 
      TSGKLKTMY EIFIKMTEN FINVVKPKA ETGEEINIK 
      YTSANFTSD IIFNAAFGM EGHTLENPD NELSKVVLN 
      VFNPSNWLF FKSILIEGL QNPGSLLRV VLSNKSMEK 
      FFMDLVRTT VTHRDENKI VRKDFLSLL LEIRDKEGL 
      SFNEIAAQS FLFFLAGAE TSSQTISYC IHQLAYHQD 
      LQDKLRKEI LDNLGTDFT KYTYDDIFK LPYLDKVFN 
      ETLRLYPVL GFLNRICVK PYKVPGTNV VIDKGTPVL 
      ISLLGLQRD PEYFPEPLK FDPERFSEG LEQVPFTFL 
      PFGDGPRFC IGMRYGKLQ TKLGIAALI SQFRFLPSP 
      KTPKFVEYD RPSKSLAMV PAKGIIVKV EKL
    CDS sequence
    • >TC014361 RA
      atgaacaac acgacagtg tttgaactt ttgtgcact 
      tttattacg atatctttg ctttcgtgg tacttgtac 
      tacgggtac tgcttcaac tactggaaa aagaaaggt 
      gttccaaca acccctccg aaattccct gccggaaac 
      atccttaag ccagtccta ggcaaagaa aatcttttc 
      gttacagtt gaagactat tacagagaa ttcaagcgc 
      gaagggcac aaacatgcc ggtttatat tttttcaac 
      ggccctgta tacctccca attgaccca gaaatcgtc 
      aagtcaatc ctcacgact gactttgac catttcgtc 
      gatcgcgga gttttctac gacgaacaa aacttccct 
      ctgagcaca cacctgttt tccatgaga ggaaccgaa 
      tggaaaagc ctccgaatg aagctttcc cccacgttc 
      acctcagga aaactcaaa accatgtac gagattttc 
      atcaaaatg accgaaaac ttcataaac gttgtgaaa 
      ccaaaagcg gaaacgggc gaagagatt aatataaaa 
      tacacgtca gcgaatttc acctccgac attattttc 
      aacgccgcg ttcggaatg gaaggccac actttggaa 
      aatccggac aacgagttg tccaaagtt gtgttaaat 
      gtcttcaac ccgtccaac tggttgttc ttcaaatcg 
      atactaata gaagggctt caaaacccg ggcagttta 
      ctacgggtg gttttaagc aacaaatcg atggaaaag 
      ttcttcatg gacctcgtc cgcacaact gtgactcat 
      cgcgacgag aataaaatt gtccggaaa gacttcctg 
      tcgctactt ttggaaatc agggacaag gaaggcttg 
      tccttcaat gaaattgcc gcccagagc tttctgttc 
      tttctggcg ggcgctgaa acttcctca cagacgata 
      agttactgc atccaccag ctggcctac caccaggat 
      ttgcaagat aagctcagg aaagaaatt ttggataac 
      ttgggtacc gactttacc aaatacacc tatgacgat 
      attttcaaa ttaccatac ttggataaa gtattcaac 
      gaaaccttg aggttgtac cccgtctta gggttcctc 
      aaccgaatt tgtgtcaag ccctacaaa gtccccggt 
      actaacgtg gtcatagat aaaggcacc ccggtttta 
      atctcgctg ttgggactt cagcgagat cctgaatat 
      tttccggag cccctgaaa tttgaccct gaaaggttt 
      agtgaaggc ctggagcaa gtccctttc acgtttttg 
      cctttcgga gacggaccc agattttgc attggtatg 
      cgttatgga aaacttcaa accaaactt ggaattgcc 
      gcacttatt tcacaattt aggtttttg ccaagtccg 
      aagactccc aaatttgtg gaatacgac aggccttca 
      aagtcacta gcgatggtg ccagcaaaa ggcataatt 
      gtgaaagtc gaaaaattg tga
    mRNA sequence
    • >TC014361 RA
      acatgttta aattcgata tgaacaaca cgacagtgt 
      ttgaacttt tgtgcactt ttattacga tatctttgc 
      tttcgtggt acttgtact acgggtact gcttcaact 
      actggaaaa agaaaggtg ttccaacaa cccctccga 
      aattccctg ccggaaaca tccttaagc cagtcctag 
      gcaaagaaa atcttttcg ttacagttg aagactatt 
      acagagaat tcaagcgcg aagggcaca aacatgccg 
      gtttatatt ttttcaacg gccctgtat acctcccaa 
      ttgacccag aaatcgtca agtcaatcc tcacgactg 
      actttgacc atttcgtcg atcgcggag ttttctacg 
      acgaacaaa acttccctc tgagcacac acctgtttt 
      ccatgagag gaaccgaat ggaaaagcc tccgaatga 
      agctttccc ccacgttca cctcaggaa aactcaaaa 
      ccatgtacg agattttca tcaaaatga ccgaaaact 
      tcataaacg ttgtgaaac caaaagcgg aaacgggcg 
      aagagatta atataaaat acacgtcag cgaatttca 
      cctccgaca ttattttca acgccgcgt tcggaatgg 
      aaggccaca ctttggaaa atccggaca acgagttgt 
      ccaaagttg tgttaaatg tcttcaacc cgtccaact 
      ggttgttct tcaaatcga tactaatag aagggcttc 
      aaaacccgg gcagtttac tacgggtgg ttttaagca 
      acaaatcga tggaaaagt tcttcatgg acctcgtcc 
      gcacaactg tgactcatc gcgacgaga ataaaattg 
      tccggaaag acttcctgt cgctacttt tggaaatca 
      gggacaagg aaggcttgt ccttcaatg aaattgccg 
      cccagagct ttctgttct ttctggcgg gcgctgaaa 
      cttcctcac agacgataa gttactgca tccaccagc 
      tggcctacc accaggatt tgcaagata agctcagga 
      aagaaattt tggataact tgggtaccg actttacca 
      aatacacct atgacgata ttttcaaat taccatact 
      tggataaag tattcaacg aaaccttga ggttgtacc 
      ccgtcttag ggttcctca accgaattt gtgtcaagc 
      cctacaaag tccccggta ctaacgtgg tcatagata 
      aaggcaccc cggttttaa tctcgctgt tgggacttc 
      agcgagatc ctgaatatt ttccggagc ccctgaaat 
      ttgaccctg aaaggttta gtgaaggcc tggagcaag 
      tccctttca cgtttttgc ctttcggag acggaccca 
      gattttgca ttggtatgc gttatggaa aacttcaaa 
      ccaaacttg gaattgccg cacttattt cacaattta 
      ggtttttgc caagtccga agactccca aatttgtgg 
      aatacgaca ggccttcaa agtcactag cgatggtgc 
      cagcaaaag gcataattg tgaaagtcg aaaaattgt 
      gattttgtt taattgttg taccattta atagttatt 
      taataaact aatttcatt atc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY