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Gene information
General information
TC014348 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9468601..9470729
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014348
    iBeetle screen iB_05615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014348 RA
      MLQIMHLFF LLLGLSPFA TSDDLPTLY EYYTTDGVS 
      SGLSVDQPY FTLNGKNIT IYSGAMHYF RVPSQYWQD 
      RLRKLRATG LNTVETYIP WNLHEFKNN VFDFGDGGS 
      DYQRLLDLD LFLTLAKQE DLFVIARPG PYICAEWEW 
      GGLPSWLLR EEAISVRTS DPVYMKYVK RYFDKLLPI 
      LAKHQFTKR GAVIAVQVE NEYASAPIE KDHAYLQQL 
      VDLMKDNGI VELLVTSDG AGYGTDGTL PGVLFQTVN 
      FGSDAKASF SKLEEMQPN KPLMAMEFY PGWFDHWSE 
      THHTVSNSS FVKNYEEVL AYPASINLY MFHGGTNFG 
      FLNGANIGS GDNSNFQPT TTSYDYDAP LSEAGDYTD 
      KYEVIRTLI KKYNPVQTK TPDPPELYE RQVYPTLAI 
      EGQLGYNDI IRQAPSTVL STSDIPMEN LPINDNSGQ 
      SYGYIVYRA SGIDIPANS VLTITGHVR DTVMVLIDG 
      VLISKPLSS LDDLNTFGF WKLDNSNIT LTTSDLVNV 
      SLDLVIENY GRANYGNFF HQFKGLTPD NRVYLNDKE 
      LQSWVIFPL EFQKSWNTK LTDWQPAGD EPTKGPGMH 
      KAVLNIDTY AERYNDTYI DMQEWNKGI VIVNGFVLG 
      RYAFIGPQQ ALYLPGAYL QHGDNEIII FEQFESAAQ 
      VKFSKDQIY KNP
    CDS sequence
    • >TC014348 RA
      atgctacag attatgcac ctttttttc ctgcttctt 
      ggtctatca ccatttgct acctctgac gacctacca 
      accctttac gaatactac acaaccgat ggagtctcc 
      tctggctta tcagtcgat caaccatac tttacccta 
      aacggtaaa aacatcacc atatacagt ggtgctatg 
      cactatttc cgggtccct tcgcaatac tggcaagac 
      cgcctccgg aaactaaga gccacaggt ctcaacact 
      gtcgaaacc tacatccct tggaacctt cacgaattc 
      aaaaacaac gttttcgac tttggagac ggcggcagc 
      gactatcag agactcctc gacttggac ctcttcctg 
      actcttgcc aaacaagaa gacttgttc gtgattgca 
      cgtccgggg ccttacatt tgcgccgag tgggaatgg 
      ggcggtctt cccagctgg ctgttgaga gaggaagcg 
      atctcagtg cgaacctct gatccagtc tacatgaaa 
      tacgtcaaa cggtacttt gacaagcta ctgccgatt 
      cttgccaag caccagttc accaaaagg ggggctgta 
      atagccgtg caagtggaa aacgaatac gcctcagcc 
      cccattgag aaagatcac gcgtatttg caacaactg 
      gtcgattta atgaaggat aatgggatt gtcgagttg 
      ttggtaact tcggatggt gcgggttat ggtaccgat 
      ggtaccctc cctggggtt cttttccaa acggtcaat 
      ttcgggtca gacgctaag gcttcgttc tcaaagctg 
      gaagaaatg caacccaat aaaccccta atggcgatg 
      gagttctat ccggggtgg ttcgaccat tggtcggag 
      acccaccac actgtgagc aacagctcg tttgtgaaa 
      aattacgaa gaagttttg gcatatcca gcgtccatc 
      aatttgtac atgtttcac ggtggtacc aactttggg 
      tttttgaac ggtgctaat atcggctcg ggggataat 
      tccaacttc caaccaact accacaagt tatgactat 
      gatgctccc ttgagtgaa gctggggat tataccgac 
      aaatacgaa gttattcga accttgatc aagaagtac 
      aatccggtt caaacgaaa acacccgat ccgccagag 
      ctgtatgag cgacaagtt tacccaact ttggcaatt 
      gagggacag ttgggttat aatgatatt attaggcag 
      gctccgagt acggtttta agtacgagt gacataccg 
      atggagaat ttaccgatc aatgataat tcggggcaa 
      agttacggc tacattgtc tacagggct tcgggtata 
      gacatccca gcaaactca gttttgacc atcacaggc 
      catgtccgt gacactgtg atggttttg atagacggt 
      gttttaatc tcgaaacca ttgtccagt ctcgacgac 
      ttgaacact ttcggtttc tggaaactc gacaattcg 
      aacataact ctgacaacc agcgatttg gttaacgta 
      agcttggac cttgtgatc gaaaattac ggacgtgcc 
      aattatggc aacttcttt caccaattc aagggtctc 
      actccagat aacagagtt tatctcaat gataaggaa 
      ttacaatcg tgggtgatt ttcccactg gaatttcag 
      aaatcttgg aacacgaaa ttaaccgac tggcagccg 
      gctggtgat gagccgacg aaaggacca gggatgcat 
      aaggccgta ctcaacatt gacacttat gctgagaga 
      tacaacgac acatacatc gatatgcag gagtggaat 
      aagggaatt gtgatagtt aatgggttt gtcttagga 
      cggtatgcg ttcattggg ccgcaacaa gccctttac 
      ctaccgggg gcgtatcta cagcatgga gacaacgaa 
      attattatt ttcgaacag ttcgaatcg gcggcccaa 
      gtgaaattt tccaaggac caaatatac aaaaatcca 
      tga
    mRNA sequence
    • >TC014348 RA
      tagataaac ttttaaaaa caggtcgaa aacagtgga 
      taaaactga tgcgaaact gtcaccatg ctacagatt 
      atgcacctt tttttcctg cttcttggt ctatcacca 
      tttgctacc tctgacgac ctaccaacc ctttacgaa 
      tactacaca accgatgga gtctcctct ggcttatca 
      gtcgatcaa ccatacttt accctaaac ggtaaaaac 
      atcaccata tacagtggt gctatgcac tatttccgg 
      gtcccttcg caatactgg caagaccgc ctccggaaa 
      ctaagagcc acaggtctc aacactgtc gaaacctac 
      atcccttgg aaccttcac gaattcaaa aacaacgtt 
      ttcgacttt ggagacggc ggcagcgac tatcagaga 
      ctcctcgac ttggacctc ttcctgact cttgccaaa 
      caagaagac ttgttcgtg attgcacgt ccggggcct 
      tacatttgc gccgagtgg gaatggggc ggtcttccc 
      agctggctg ttgagagag gaagcgatc tcagtgcga 
      acctctgat ccagtctac atgaaatac gtcaaacgg 
      tactttgac aagctactg ccgattctt gccaagcac 
      cagttcacc aaaaggggg gctgtaata gccgtgcaa 
      gtggaaaac gaatacgcc tcagccccc attgagaaa 
      gatcacgcg tatttgcaa caactggtc gatttaatg 
      aaggataat gggattgtc gagttgttg gtaacttcg 
      gatggtgcg ggttatggt accgatggt accctccct 
      ggggttctt ttccaaacg gtcaatttc gggtcagac 
      gctaaggct tcgttctca aagctggaa gaaatgcaa 
      cccaataaa cccctaatg gcgatggag ttctatccg 
      gggtggttc gaccattgg tcggagacc caccacact 
      gtgagcaac agctcgttt gtgaaaaat tacgaagaa 
      gttttggca tatccagcg tccatcaat ttgtacatg 
      tttcacggt ggtaccaac tttgggttt ttgaacggt 
      gctaatatc ggctcgggg gataattcc aacttccaa 
      ccaactacc acaagttat gactatgat gctcccttg 
      agtgaagct ggggattat accgacaaa tacgaagtt 
      attcgaacc ttgatcaag aagtacaat ccggttcaa 
      acgaaaaca cccgatccg ccagagctg tatgagcga 
      caagtttac ccaactttg gcaattgag ggacagttg 
      ggttataat gatattatt aggcaggct ccgagtacg 
      gttttaagt acgagtgac ataccgatg gagaattta 
      ccgatcaat gataattcg gggcaaagt tacggctac 
      attgtctac agggcttcg ggtatagac atcccagca 
      aactcagtt ttgaccatc acaggccat gtccgtgac 
      actgtgatg gttttgata gacggtgtt ttaatctcg 
      aaaccattg tccagtctc gacgacttg aacactttc 
      ggtttctgg aaactcgac aattcgaac ataactctg 
      acaaccagc gatttggtt aacgtaagc ttggacctt 
      gtgatcgaa aattacgga cgtgccaat tatggcaac 
      ttctttcac caattcaag ggtctcact ccagataac 
      agagtttat ctcaatgat aaggaatta caatcgtgg 
      gtgattttc ccactggaa tttcagaaa tcttggaac 
      acgaaatta accgactgg cagccggct ggtgatgag 
      ccgacgaaa ggaccaggg atgcataag gccgtactc 
      aacattgac acttatgct gagagatac aacgacaca 
      tacatcgat atgcaggag tggaataag ggaattgtg 
      atagttaat gggtttgtc ttaggacgg tatgcgttc 
      attgggccg caacaagcc ctttaccta ccgggggcg 
      tatctacag catggagac aacgaaatt attattttc 
      gaacagttc gaatcggcg gcccaagtg aaattttcc 
      aaggaccaa atatacaaa aatccatga gttgagttc 
      gtgtaattt tgaataaaa ttatgattg caag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY