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Gene information
General information
TC014334 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9257800..9262502
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014334
    iBeetle screen iB_05612

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014334 RA
      MKLKIALAK SPSHSAAVT CVGWSSTEE VYSIGDDNQ 
      LLSWSVSNN QSKKVAQLG KDLFAIDMQ FLPRTSGTL 
      SKHGDLILV TSADGKFHI MNRSGRIER SVEAHKGAI 
      LVGQWGNDG AGLLTAGED GCIKIWSRS GMLRSIVVS 
      SDNSVYGAS WAPDSQSIA YTQGKFIVI KQLAPNTKP 
      LRWKAHEEL VLCLAWSPA SELIVSGGE DCQYKVWDG 
      QGRQLFYSG PHDNHITSI AWAPNGDLF AVGSYNTVR 
      LCDYSGWSR SLEKPTTGC VYKMAWSGD GTQLAGACA 
      NGHVFFAHI VERHVHYKN FTASVTERK VVTVRNVVD 
      DTFEQLELP ERVIQLALK YTHLVATTP TQCYIYSTN 
      NWNTPAIFD LKDGSVILL LLAEKHMLL VEKNTIGIY 
      NYQGRLIAS PRWPNMRLD SLRATHISL SSDTLVVRD 
      SIDHKIVHV IDLSSVRST TESTSTVQH FLPILQVAL 
      DQWGPPSAR MLALVNKTK DLYVVAPRS NNKIFHKLG 
      RKTESFQWN SEANIIATI QDAQLVVWY CPTAAFNST 
      LLKLCSMQY GAPELGRNP RISDFVGNS VSIRRADGS 
      LINVPISPF PSILHRCIQ DNKWSDALN LCRSTNDET 
      LWACLAVLT TQSSADVID IAEEAFANI NHFDKVFYI 
      QYAKSLTNK IQQKAAIAL LGGAQQEAE AILLHNGMV 
      FQAIYSNIQ MHNWRRALD LAIKHKTHI DTVLFLRQK 
      YLDSLGKPE SDNKFSSMN ETIQIDAEK VQQKISAEL 
      QK
    CDS sequence
    • >TC014334 RA
      atgaaatta aaaattgca ctggctaaa agcccgtcg 
      cattcggcc gctgtgact tgcgtcggc tggagcagc 
      acggaggag gtctactcc ataggcgac gacaaccaa 
      ctgctttca tggtcggtg tccaacaat cagtcgaag 
      aaagtggcc cagctgggc aaggacctg ttcgccatc 
      gacatgcag ttcctgccc aggaccagc ggcaccttg 
      agcaagcac ggagatttg atactggta acctcagcc 
      gatgggaaa ttccacata atgaaccgc tcgggcaga 
      atcgagcgc agcgtggag gcccataaa ggggcgatc 
      ttagtgggg caatggggc aacgacggg gcagggctg 
      ctgactgca ggagaagac ggctgcatc aagatctgg 
      tctcggagc ggaatgtta cgatctatt gtcgtctcc 
      agcgataat tcagtttac ggggcctct tgggccccc 
      gacagtcaa agcatcgcg tacactcag ggcaaattc 
      atcgtgata aaacagcta gcaccgaac actaaacct 
      ttacggtgg aaagctcat gaagaatta gtgctgtgt 
      ttagcatgg agtccggct tcggaactt atagtttcc 
      gggggcgag gactgccaa tacaaagtc tgggatgga 
      caaggacgg cagttgttc tacagcggc ccgcacgac 
      aatcacatt acctcgatt gcttgggcc ccaaatggg 
      gatcttttc gcagtcggg tcctataat acggtccgg 
      ctgtgcgac tactccggg tggtcaaga tctctcgaa 
      aaaccaacc acgggctgc gtttacaaa atggcatgg 
      tctggagat ggtactcaa ctagcgggt gcttgtgct 
      aatggtcac gtcttcttc gcacacata gtggaaaga 
      cacgttcat tacaaaaat ttcactgct tcagtaacc 
      gaacgtaaa gtcgtgacg gttagaaat gttgtggac 
      gatactttc gaacaactc gaattacca gaaagagta 
      atacaattg gctctaaaa tacacgcat ttggttgca 
      acaacgccc acacaatgt tacatttat agcaccaat 
      aactggaat acaccagca atttttgac ttgaaggac 
      ggttctgta attttatta ctactagcc gaaaaacac 
      atgttactg gttgaaaaa aacaccatc ggcatctac 
      aattatcaa gggagactt attgcttcg cccagatgg 
      cccaacatg agattagac tctttaaga gctactcat 
      attagctta tcgtccgat actttagtc gtacgagac 
      tctatagac cataaaatc gtgcatgta attgactta 
      agcagtgtg agaagtacc acagaatcc acttcaacg 
      gttcaacac tttctgccc attttacaa gtcgctctt 
      gaccagtgg ggaccacca agtgcccga atgttagca 
      ctggtgaac aaaactaaa gacctatac gtcgttgca 
      ccaagaagt aataataaa atattccat aaactaggt 
      aggaaaaca gagtcgttt cagtggaat tcagaagca 
      aacatcatt gcaacgatt caagatgca cagttggtt 
      gtttggtat tgtccgact gctgctttt aattcaact 
      cttctgaaa ttatgttca atgcaatac ggcgctcca 
      gagctggga cgtaatccg agaataagt gatttcgtg 
      gggaattcg gtgtcaatt cggagagct gatggttct 
      ttaatcaac gtcccgatt tctccattc ccttcaata 
      cttcataga tgtattcaa gacaacaaa tggagcgac 
      gctttaaat ctatgtaga tcaacaaac gacgagact 
      ttgtgggca tgtcttgca gttttgacg actcagtcc 
      agtgccgat gttatcgac atagcggag gaagctttc 
      gccaatatt aatcatttt gataaagtg ttttacatc 
      caatatgca aagagtttg acgaacaaa attcaacaa 
      aaagccgca attgccctc cttggtggg gcgcagcaa 
      gaagctgag gcaattctt ttgcacaat gggatggtg 
      tttcaagcc atttacagc aacattcaa atgcacaac 
      tggaggcgg gctctggat ttggcaatt aaacacaaa 
      acgcatatt gacacagtg ttgttctta aggcaaaag 
      tatttagac tcgttggga aaacccgaa agtgataac 
      aaattttcg tcaatgaat gaaactata caaatagat 
      gcggagaaa gtgcaacaa aaaatttca gccgaactg 
      caaaagtaa
    mRNA sequence
    • >TC014334 RA
      cagcaaacg aaacttcat gttcacaaa gtaaacatt 
      attttgtgc attattcat ttatttcaa aaaactaga 
      agtaatgaa attaaaaat tgcactggc taaaagccc 
      gtcgcattc ggccgctgt gacttgcgt cggctggag 
      cagcacgga ggaggtcta ctccatagg cgacgacaa 
      ccaactgct ttcatggtc ggtgtccaa caatcagtc 
      gaagaaagt ggcccagct gggcaagga cctgttcgc 
      catcgacat gcagttcct gcccaggac cagcggcac 
      cttgagcaa gcacggaga tttgatact ggtaacctc 
      agccgatgg gaaattcca cataatgaa ccgctcggg 
      cagaatcga gcgcagcgt ggaggccca taaaggggc 
      gatcttagt ggggcaatg gggcaacga cggggcagg 
      gctgctgac tgcaggaga agacggctg catcaagat 
      ctggtctcg gagcggaat gttacgatc tattgtcgt 
      ctccagcga taattcagt ttacggggc ctcttgggc 
      ccccgacag tcaaagcat cgcgtacac tcagggcaa 
      attcatcgt gataaaaca gctagcacc gaacactaa 
      acctttacg gtggaaagc tcatgaaga attagtgct 
      gtgtttagc atggagtcc ggcttcgga acttatagt 
      ttccggggg cgaggactg ccaatacaa agtctggga 
      tggacaagg acggcagtt gttctacag cggcccgca 
      cgacaatca cattacctc gattgcttg ggccccaaa 
      tggggatct tttcgcagt cgggtccta taatacggt 
      ccggctgtg cgactactc cgggtggtc aagatctct 
      cgaaaaacc aaccacggg ctgcgttta caaaatggc 
      atggtctgg agatggtac tcaactagc gggtgcttg 
      tgctaatgg tcacgtctt cttcgcaca catagtgga 
      aagacacgt tcattacaa aaatttcac tgcttcagt 
      aaccgaacg taaagtcgt gacggttag aaatgttgt 
      ggacgatac tttcgaaca actcgaatt accagaaag 
      agtaataca attggctct aaaatacac gcatttggt 
      tgcaacaac gcccacaca atgttacat ttatagcac 
      caataactg gaatacacc agcaatttt tgacttgaa 
      ggacggttc tgtaatttt attactact agccgaaaa 
      acacatgtt actggttga aaaaaacac catcggcat 
      ctacaatta tcaagggag acttattgc ttcgcccag 
      atggcccaa catgagatt agactcttt aagagctac 
      tcatattag cttatcgtc cgatacttt agtcgtacg 
      agactctat agaccataa aatcgtgca tgtaattga 
      cttaagcag tgtgagaag taccacaga atccacttc 
      aacggttca acactttct gcccatttt acaagtcgc 
      tcttgacca gtggggacc accaagtgc ccgaatgtt 
      agcactggt gaacaaaac taaagacct atacgtcgt 
      tgcaccaag aagtaataa taaaatatt ccataaact 
      aggtaggaa aacagagtc gtttcagtg gaattcaga 
      agcaaacat cattgcaac gattcaaga tgcacagtt 
      ggttgtttg gtattgtcc gactgctgc ttttaattc 
      aactcttct gaaattatg ttcaatgca atacggcgc 
      tccagagct gggacgtaa tccgagaat aagtgattt 
      cgtggggaa ttcggtgtc aattcggag agctgatgg 
      ttctttaat caacgtccc gatttctcc attcccttc 
      aatacttca tagatgtat tcaagacaa caaatggag 
      cgacgcttt aaatctatg tagatcaac aaacgacga 
      gactttgtg ggcatgtct tgcagtttt gacgactca 
      gtccagtgc cgatgttat cgacatagc ggaggaagc 
      tttcgccaa tattaatca ttttgataa agtgtttta 
      catccaata tgcaaagag tttgacgaa caaaattca 
      acaaaaagc cgcaattgc cctccttgg tggggcgca 
      gcaagaagc tgaggcaat tcttttgca caatgggat 
      ggtgtttca agccattta cagcaacat tcaaatgca 
      caactggag gcgggctct ggatttggc aattaaaca 
      caaaacgca tattgacac agtgttgtt cttaaggca 
      aaagtattt agactcgtt gggaaaacc cgaaagtga 
      taacaaatt ttcgtcaat gaatgaaac tatacaaat 
      agatgcgga gaaagtgca acaaaaaat ttcagccga 
      actgcaaaa gtaaatatc acattgggt ttttgttgt 
      taaaataat aaaacgatt agttcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY