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Gene information
General information
TC014330 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9158412..9183164
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014330
    iBeetle screen iB_05610

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 9

    Comment: all animals show 8 instead of 10 flagellomeres

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014330 RA
      MVPKLVQVL SVLCVVQYG KAARWSHSA MLSPDYRLL 
      WSLGPGPQD ITFELQVRT LGYVGFGFS KDGRMTGAD 
      MVVGWVDKG QVYFQDRHV KDSPVSSRD REPEVDPSQ 
      DYQLLLGYE NHTHTVLRF RRKLDTCDH HDIPITNDT 
      MRVVWAFHS EEPVGGSVG PNSLPQHDV EFRGTQSLY 
      LVQRADQEA PGPEETARI WELRNPAVE PPAPAENLY 
      WCRVFKIPV ITKKHHLIR YEPLQGIRG PTGLQHVVL 
      YECQDSPQV AQLADTPGR QCYEPHTQP LSCNTVVAS 
      WARGSEGFS FPPEAGYPL EPATSRYYL LETHYISPT 
      DGSLGSLDG SGLRLYYTP ELRRHDAGV ISIGMDPNW 
      RHIIPPGQQ RVVSSGHCV SECTKQAFP HNGINMFAV 
      VMKTHRIGR QVALKHIRG NVEQPPIAA DDNLDADYQ 
      EYRRLGIPV KILPGDHLI AECTYNSSG RTAITLGGL 
      TSREETCLV MGLYYPKQR SLAACHSLP SLPTVLHSL 
      GIQELSPGS NPVRIAAPP ELAGMTLES RLVSYDWDN 
      HFHNFQEAT MKGSFKPLC RTAQSTILP GTDKEFHYP 
      NITIAYVRN NADQCNSYK RYSTEGWPG GTPVMELEG 
      NHIEGAARS KVSRSSSSP TAEESLGLS RVNSACRCY 
      SFQTVLVLC VLLVWSNH
    CDS sequence
    • >TC014330 RA
      atggtccct aaattagtt caagtgcta agtgttttg 
      tgtgttgtg caatacgga aaagcggct agatggtca 
      cactctgca atgctcagc ccggactat cgacttctt 
      tggtcgtta gggccggga cctcaagac atcacgttt 
      gaattgcaa gtgaggact ctgggatat gttgggttt 
      ggcttttca aaggatgga cgcatgaca ggggctgac 
      atggtcgtc ggatgggtg gacaaagga caagtctac 
      ttccaggat cgccatgtt aaggattct cctgtaagt 
      tctcgggat agagaaccg gaagtggat ccaagccag 
      gattatcaa cttctgttg ggttatgag aaccacaca 
      catactgtg ttacgattc aggaggaaa cttgatact 
      tgtgaccat catgacatc cccatcacg aacgatacg 
      atgagggta gtgtgggct ttccactcg gaggagcct 
      gtgggggga tcagtgggt ccaaactca ttgccacaa 
      catgacgtc gaattcaga ggaacccag tcgctttat 
      ttggttcag agggccgat caggaggct cctgggcct 
      gaagaaacg gcaagaatt tgggaactg cggaacccg 
      gcggtggaa ccgccagca cctgctgaa aacttgtac 
      tggtgccga gttttcaaa attcctgtc attaccaag 
      aagcaccac ttaattcgg tacgagcca cttcaggga 
      atcagaggc ccgaccgga ttgcagcac gtcgtgctg 
      tacgagtgt caggacagc ccccaggtc gcgcagctg 
      gccgacacc cccggccgg cagtgctac gagccccac 
      acccagccc ctttcgtgc aatacggtc gttgcgagt 
      tgggcgcga ggctctgaa ggttttagt ttcccaccc 
      gaagccggt tatccccta gaacccgcc acatcccgc 
      tactacttg ctggaaacg cactacatc agccccaca 
      gatggcagt ttggggtct ctcgacggg tccggcttg 
      aggctgtat tataccccc gaattacgc cggcacgac 
      gctggcgtt atatcaata gggatggat ccgaactgg 
      agacatata ataccgcca ggacaacaa agagttgtc 
      tcttcagga cattgcgtg tcggagtgc accaaacaa 
      gctttcccc cataatggc ataaacatg ttcgctgtt 
      gttatgaaa acacaccga attggacga caggttgct 
      ttgaagcac atcagaggc aatgtggag cagcctccc 
      attgcggcg gatgacaat ctcgatgct gattaccag 
      gagtatcgc aggctcgga attcccgtc aaaatacta 
      cccggcgac catcttatt gccgagtgt acttacaac 
      agctcgggc cggacggcc atcacccta ggaggactc 
      acaagtcgc gaggaaacc tgcctggtt atggggctc 
      tattacccg aaacagcgc tctttagcg gcatgtcac 
      agtttgccg agcctgccg acagtcctg cattctttg 
      ggaatacaa gaactgagc ccggggtcg aaccccgtg 
      aggatagcc gcccctcca gagttggcc gggatgact 
      ctggaatcc aggttggtg tcttatgac tgggacaat 
      catttccac aattttcaa gaagcaacg atgaaaggt 
      tccttcaag ccattgtgc cggactgcg caatcgacg 
      atattgccg ggcacagat aaggaattc cattacccg 
      aatattacg atagcatac gtccgtaac aatgcagac 
      caatgtaac agctacaaa aggtatagc acagaaggc 
      tggccggga ggaacgccc gtcatggaa ttggaaggt 
      aatcatata gaaggagca gctcgtagc aaagtttcg 
      cgtagcagc agcagtcca accgctgaa gaatcgctt 
      ggattgagt agagtgaac tctgcttgt agatgttat 
      tccttccag actgtgtta gtgctctgt gttttgttg 
      gtttggtcc aaccactga
    mRNA sequence
    • >TC014330 RA
      taggcagag aaacgtttc agcagtctg aaataaacc 
      gtgatagag ggagtatga gtcgctcgc ttcgctatt 
      ccgtcaatt ccgggtgca tattcctaa cactatcaa 
      agtaaaaat gcgtcggac ggctaatga agctacgtg 
      ataagtgaa taatcaatg attcgtgtt tgagttgga 
      aagttcctc gggtttcgg gagctttgc ccggagttt 
      gtttgtgtt tttcaccgt tgtatggaa atcgtgacc 
      gctttgtgt tccggaaac attctttgt gacttttca 
      ggcaatatt tggattatc tctcgtggt ttttatatg 
      aagcctccg tgcaactgc taacaatgg tccctaaat 
      tagttcaag tgctaagtg ttttgtgtg ttgtgcaat 
      acggaaaag cggctagat ggtcacact ctgcaatgc 
      tcagcccgg actatcgac ttctttggt cgttagggc 
      cgggacctc aagacatca cgtttgaat tgcaagtga 
      ggactctgg gatatgttg ggtttggct tttcaaagg 
      atggacgca tgacagggg ctgacatgg tcgtcggat 
      gggtggaca aaggacaag tctacttcc aggatcgcc 
      atgttaagg attctcctg taagttctc gggatagag 
      aaccggaag tggatccaa gccaggatt atcaacttc 
      tgttgggtt atgagaacc acacacata ctgtgttac 
      gattcagga ggaaacttg atacttgtg accatcatg 
      acatcccca tcacgaacg atacgatga gggtagtgt 
      gggctttcc actcggagg agcctgtgg ggggatcag 
      tgggtccaa actcattgc cacaacatg acgtcgaat 
      tcagaggaa cccagtcgc tttatttgg ttcagaggg 
      ccgatcagg aggctcctg ggcctgaag aaacggcaa 
      gaatttggg aactgcgga acccggcgg tggaaccgc 
      cagcacctg ctgaaaact tgtactggt gccgagttt 
      tcaaaattc ctgtcatta ccaagaagc accacttaa 
      ttcggtacg agccacttc agggaatca gaggcccga 
      ccggattgc agcacgtcg tgctgtacg agtgtcagg 
      acagccccc aggtcgcgc agctggccg acacccccg 
      gccggcagt gctacgagc cccacaccc agccccttt 
      cgtgcaata cggtcgttg cgagttggg cgcgaggct 
      ctgaaggtt ttagtttcc cacccgaag ccggttatc 
      ccctagaac ccgccacat cccgctact acttgctgg 
      aaacgcact acatcagcc ccacagatg gcagtttgg 
      ggtctctcg acgggtccg gcttgaggc tgtattata 
      cccccgaat tacgccggc acgacgctg gcgttatat 
      caataggga tggatccga actggagac atataatac 
      cgccaggac aacaaagag ttgtctctt caggacatt 
      gcgtgtcgg agtgcacca aacaagctt tcccccata 
      atggcataa acatgttcg ctgttgtta tgaaaacac 
      accgaattg gacgacagg ttgctttga agcacatca 
      gaggcaatg tggagcagc ctcccattg cggcggatg 
      acaatctcg atgctgatt accaggagt atcgcaggc 
      tcggaattc ccgtcaaaa tactacccg gcgaccatc 
      ttattgccg agtgtactt acaacagct cgggccgga 
      cggccatca ccctaggag gactcacaa gtcgcgagg 
      aaacctgcc tggttatgg ggctctatt acccgaaac 
      agcgctctt tagcggcat gtcacagtt tgccgagcc 
      tgccgacag tcctgcatt ctttgggaa tacaagaac 
      tgagcccgg ggtcgaacc ccgtgagga tagccgccc 
      ctccagagt tggccggga tgactctgg aatccaggt 
      tggtgtctt atgactggg acaatcatt tccacaatt 
      ttcaagaag caacgatga aaggttcct tcaagccat 
      tgtgccgga ctgcgcaat cgacgatat tgccgggca 
      cagataagg aattccatt acccgaata ttacgatag 
      catacgtcc gtaacaatg cagaccaat gtaacagct 
      acaaaaggt atagcacag aaggctggc cgggaggaa 
      cgcccgtca tggaattgg aaggtaatc atatagaag 
      gagcagctc gtagcaaag tttcgcgta gcagcagca 
      gtccaaccg ctgaagaat cgcttggat tgagtagag 
      tgaactctg cttgtagat gttattcct tccagactg 
      tgttagtgc tctgtgttt tgttggttt ggtccaacc 
      actgaccta gtgatttaa cttagtgac ctcatgact 
      gatttttat attcctgaa tcgtacatg gtgatagca 
      acgactgct gaattttta tttattgac attgttgtg 
      aattatttg agtcataca tttactagt ccgttcggc 
      cagatcata cgtggtttt tgaatgatt tctctcttt 
      ttctataaa tgtatttag taaagcgac taatccaag 
      tgcaatagt ttccggata cggaaaaat cgctttgct 
      ggaaaatat gtagcggta gtggatgcc gaatgggac 
      agttgaata cgccgcgac aggtctgtg atgaaagta 
      gtaataagc acttttcat gtttctttt ctttaggta 
      atgacgtct cggttttgc tactgttgt tagtttact 
      cttctttaa cccgtcacg agggttgta tttgtattt 
      atatgtaaa tttaatatc ctgtgtata tttgtagtc 
      tcgactggt gctatattt tttattctt ttgtatgtt 
      atttaattt cgactttcc ttagtacga taaatttct 
      tgtacagtg tagataact tgtgtatat tttataaat 
      aaacattaa taaacaaaa tctagcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY