General information | |||
TC014330 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 9158412..9183164 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014330 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05610 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)
Comment: all animals show 8 instead of 10 flagellomeres |
- >TC014330 RA
MVPKLVQVL SVLCVVQYG KAARWSHSA MLSPDYRLL WSLGPGPQD ITFELQVRT LGYVGFGFS KDGRMTGAD MVVGWVDKG QVYFQDRHV KDSPVSSRD REPEVDPSQ DYQLLLGYE NHTHTVLRF RRKLDTCDH HDIPITNDT MRVVWAFHS EEPVGGSVG PNSLPQHDV EFRGTQSLY LVQRADQEA PGPEETARI WELRNPAVE PPAPAENLY WCRVFKIPV ITKKHHLIR YEPLQGIRG PTGLQHVVL YECQDSPQV AQLADTPGR QCYEPHTQP LSCNTVVAS WARGSEGFS FPPEAGYPL EPATSRYYL LETHYISPT DGSLGSLDG SGLRLYYTP ELRRHDAGV ISIGMDPNW RHIIPPGQQ RVVSSGHCV SECTKQAFP HNGINMFAV VMKTHRIGR QVALKHIRG NVEQPPIAA DDNLDADYQ EYRRLGIPV KILPGDHLI AECTYNSSG RTAITLGGL TSREETCLV MGLYYPKQR SLAACHSLP SLPTVLHSL GIQELSPGS NPVRIAAPP ELAGMTLES RLVSYDWDN HFHNFQEAT MKGSFKPLC RTAQSTILP GTDKEFHYP NITIAYVRN NADQCNSYK RYSTEGWPG GTPVMELEG NHIEGAARS KVSRSSSSP TAEESLGLS RVNSACRCY SFQTVLVLC VLLVWSNH
- >TC014330 RA
atggtccct aaattagtt caagtgcta agtgttttg tgtgttgtg caatacgga aaagcggct agatggtca cactctgca atgctcagc ccggactat cgacttctt tggtcgtta gggccggga cctcaagac atcacgttt gaattgcaa gtgaggact ctgggatat gttgggttt ggcttttca aaggatgga cgcatgaca ggggctgac atggtcgtc ggatgggtg gacaaagga caagtctac ttccaggat cgccatgtt aaggattct cctgtaagt tctcgggat agagaaccg gaagtggat ccaagccag gattatcaa cttctgttg ggttatgag aaccacaca catactgtg ttacgattc aggaggaaa cttgatact tgtgaccat catgacatc cccatcacg aacgatacg atgagggta gtgtgggct ttccactcg gaggagcct gtgggggga tcagtgggt ccaaactca ttgccacaa catgacgtc gaattcaga ggaacccag tcgctttat ttggttcag agggccgat caggaggct cctgggcct gaagaaacg gcaagaatt tgggaactg cggaacccg gcggtggaa ccgccagca cctgctgaa aacttgtac tggtgccga gttttcaaa attcctgtc attaccaag aagcaccac ttaattcgg tacgagcca cttcaggga atcagaggc ccgaccgga ttgcagcac gtcgtgctg tacgagtgt caggacagc ccccaggtc gcgcagctg gccgacacc cccggccgg cagtgctac gagccccac acccagccc ctttcgtgc aatacggtc gttgcgagt tgggcgcga ggctctgaa ggttttagt ttcccaccc gaagccggt tatccccta gaacccgcc acatcccgc tactacttg ctggaaacg cactacatc agccccaca gatggcagt ttggggtct ctcgacggg tccggcttg aggctgtat tataccccc gaattacgc cggcacgac gctggcgtt atatcaata gggatggat ccgaactgg agacatata ataccgcca ggacaacaa agagttgtc tcttcagga cattgcgtg tcggagtgc accaaacaa gctttcccc cataatggc ataaacatg ttcgctgtt gttatgaaa acacaccga attggacga caggttgct ttgaagcac atcagaggc aatgtggag cagcctccc attgcggcg gatgacaat ctcgatgct gattaccag gagtatcgc aggctcgga attcccgtc aaaatacta cccggcgac catcttatt gccgagtgt acttacaac agctcgggc cggacggcc atcacccta ggaggactc acaagtcgc gaggaaacc tgcctggtt atggggctc tattacccg aaacagcgc tctttagcg gcatgtcac agtttgccg agcctgccg acagtcctg cattctttg ggaatacaa gaactgagc ccggggtcg aaccccgtg aggatagcc gcccctcca gagttggcc gggatgact ctggaatcc aggttggtg tcttatgac tgggacaat catttccac aattttcaa gaagcaacg atgaaaggt tccttcaag ccattgtgc cggactgcg caatcgacg atattgccg ggcacagat aaggaattc cattacccg aatattacg atagcatac gtccgtaac aatgcagac caatgtaac agctacaaa aggtatagc acagaaggc tggccggga ggaacgccc gtcatggaa ttggaaggt aatcatata gaaggagca gctcgtagc aaagtttcg cgtagcagc agcagtcca accgctgaa gaatcgctt ggattgagt agagtgaac tctgcttgt agatgttat tccttccag actgtgtta gtgctctgt gttttgttg gtttggtcc aaccactga
- >TC014330 RA
taggcagag aaacgtttc agcagtctg aaataaacc gtgatagag ggagtatga gtcgctcgc ttcgctatt ccgtcaatt ccgggtgca tattcctaa cactatcaa agtaaaaat gcgtcggac ggctaatga agctacgtg ataagtgaa taatcaatg attcgtgtt tgagttgga aagttcctc gggtttcgg gagctttgc ccggagttt gtttgtgtt tttcaccgt tgtatggaa atcgtgacc gctttgtgt tccggaaac attctttgt gacttttca ggcaatatt tggattatc tctcgtggt ttttatatg aagcctccg tgcaactgc taacaatgg tccctaaat tagttcaag tgctaagtg ttttgtgtg ttgtgcaat acggaaaag cggctagat ggtcacact ctgcaatgc tcagcccgg actatcgac ttctttggt cgttagggc cgggacctc aagacatca cgtttgaat tgcaagtga ggactctgg gatatgttg ggtttggct tttcaaagg atggacgca tgacagggg ctgacatgg tcgtcggat gggtggaca aaggacaag tctacttcc aggatcgcc atgttaagg attctcctg taagttctc gggatagag aaccggaag tggatccaa gccaggatt atcaacttc tgttgggtt atgagaacc acacacata ctgtgttac gattcagga ggaaacttg atacttgtg accatcatg acatcccca tcacgaacg atacgatga gggtagtgt gggctttcc actcggagg agcctgtgg ggggatcag tgggtccaa actcattgc cacaacatg acgtcgaat tcagaggaa cccagtcgc tttatttgg ttcagaggg ccgatcagg aggctcctg ggcctgaag aaacggcaa gaatttggg aactgcgga acccggcgg tggaaccgc cagcacctg ctgaaaact tgtactggt gccgagttt tcaaaattc ctgtcatta ccaagaagc accacttaa ttcggtacg agccacttc agggaatca gaggcccga ccggattgc agcacgtcg tgctgtacg agtgtcagg acagccccc aggtcgcgc agctggccg acacccccg gccggcagt gctacgagc cccacaccc agccccttt cgtgcaata cggtcgttg cgagttggg cgcgaggct ctgaaggtt ttagtttcc cacccgaag ccggttatc ccctagaac ccgccacat cccgctact acttgctgg aaacgcact acatcagcc ccacagatg gcagtttgg ggtctctcg acgggtccg gcttgaggc tgtattata cccccgaat tacgccggc acgacgctg gcgttatat caataggga tggatccga actggagac atataatac cgccaggac aacaaagag ttgtctctt caggacatt gcgtgtcgg agtgcacca aacaagctt tcccccata atggcataa acatgttcg ctgttgtta tgaaaacac accgaattg gacgacagg ttgctttga agcacatca gaggcaatg tggagcagc ctcccattg cggcggatg acaatctcg atgctgatt accaggagt atcgcaggc tcggaattc ccgtcaaaa tactacccg gcgaccatc ttattgccg agtgtactt acaacagct cgggccgga cggccatca ccctaggag gactcacaa gtcgcgagg aaacctgcc tggttatgg ggctctatt acccgaaac agcgctctt tagcggcat gtcacagtt tgccgagcc tgccgacag tcctgcatt ctttgggaa tacaagaac tgagcccgg ggtcgaacc ccgtgagga tagccgccc ctccagagt tggccggga tgactctgg aatccaggt tggtgtctt atgactggg acaatcatt tccacaatt ttcaagaag caacgatga aaggttcct tcaagccat tgtgccgga ctgcgcaat cgacgatat tgccgggca cagataagg aattccatt acccgaata ttacgatag catacgtcc gtaacaatg cagaccaat gtaacagct acaaaaggt atagcacag aaggctggc cgggaggaa cgcccgtca tggaattgg aaggtaatc atatagaag gagcagctc gtagcaaag tttcgcgta gcagcagca gtccaaccg ctgaagaat cgcttggat tgagtagag tgaactctg cttgtagat gttattcct tccagactg tgttagtgc tctgtgttt tgttggttt ggtccaacc actgaccta gtgatttaa cttagtgac ctcatgact gatttttat attcctgaa tcgtacatg gtgatagca acgactgct gaattttta tttattgac attgttgtg aattatttg agtcataca tttactagt ccgttcggc cagatcata cgtggtttt tgaatgatt tctctcttt ttctataaa tgtatttag taaagcgac taatccaag tgcaatagt ttccggata cggaaaaat cgctttgct ggaaaatat gtagcggta gtggatgcc gaatgggac agttgaata cgccgcgac aggtctgtg atgaaagta gtaataagc acttttcat gtttctttt ctttaggta atgacgtct cggttttgc tactgttgt tagtttact cttctttaa cccgtcacg agggttgta tttgtattt atatgtaaa tttaatatc ctgtgtata tttgtagtc tcgactggt gctatattt tttattctt ttgtatgtt atttaattt cgactttcc ttagtacga taaatttct tgtacagtg tagataact tgtgtatat tttataaat aaacattaa taaacaaaa tctagcaat
