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Gene information
General information
TC014330 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9158412..9183164
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014330
    iBeetle screen iB_05610

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    flagellomeres number decreased - Number of animals showing the phenotype: 9

    Comment: all animals show 8 instead of 10 flagellomeres

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014330 RA
      MVPKLVQVL SVLCVVQYG KAARWSHSA MLSPDYRLL 
WSLGPGPQD ITFELQVRT LGYVGFGFS KDGRMTGAD 
MVVGWVDKG QVYFQDRHV KDSPVSSRD REPEVDPSQ 
DYQLLLGYE NHTHTVLRF RRKLDTCDH HDIPITNDT 
MRVVWAFHS EEPVGGSVG PNSLPQHDV EFRGTQSLY 
LVQRADQEA PGPEETARI WELRNPAVE PPAPAENLY 
WCRVFKIPV ITKKHHLIR YEPLQGIRG PTGLQHVVL 
YECQDSPQV AQLADTPGR QCYEPHTQP LSCNTVVAS 
WARGSEGFS FPPEAGYPL EPATSRYYL LETHYISPT 
DGSLGSLDG SGLRLYYTP ELRRHDAGV ISIGMDPNW 
RHIIPPGQQ RVVSSGHCV SECTKQAFP HNGINMFAV 
VMKTHRIGR QVALKHIRG NVEQPPIAA DDNLDADYQ 
EYRRLGIPV KILPGDHLI AECTYNSSG RTAITLGGL 
TSREETCLV MGLYYPKQR SLAACHSLP SLPTVLHSL 
GIQELSPGS NPVRIAAPP ELAGMTLES RLVSYDWDN 
HFHNFQEAT MKGSFKPLC RTAQSTILP GTDKEFHYP 
NITIAYVRN NADQCNSYK RYSTEGWPG GTPVMELEG 
NHIEGAARS KVSRSSSSP TAEESLGLS RVNSACRCY 
SFQTVLVLC VLLVWSNH
    CDS sequence
    • >TC014330 RA
      atggtccct aaattagtt caagtgcta agtgttttg 
tgtgttgtg caatacgga aaagcggct agatggtca 
cactctgca atgctcagc ccggactat cgacttctt 
tggtcgtta gggccggga cctcaagac atcacgttt 
gaattgcaa gtgaggact ctgggatat gttgggttt 
ggcttttca aaggatgga cgcatgaca ggggctgac 
atggtcgtc ggatgggtg gacaaagga caagtctac 
ttccaggat cgccatgtt aaggattct cctgtaagt 
tctcgggat agagaaccg gaagtggat ccaagccag 
gattatcaa cttctgttg ggttatgag aaccacaca 
catactgtg ttacgattc aggaggaaa cttgatact 
tgtgaccat catgacatc cccatcacg aacgatacg 
atgagggta gtgtgggct ttccactcg gaggagcct 
gtgggggga tcagtgggt ccaaactca ttgccacaa 
catgacgtc gaattcaga ggaacccag tcgctttat 
ttggttcag agggccgat caggaggct cctgggcct 
gaagaaacg gcaagaatt tgggaactg cggaacccg 
gcggtggaa ccgccagca cctgctgaa aacttgtac 
tggtgccga gttttcaaa attcctgtc attaccaag 
aagcaccac ttaattcgg tacgagcca cttcaggga 
atcagaggc ccgaccgga ttgcagcac gtcgtgctg 
tacgagtgt caggacagc ccccaggtc gcgcagctg 
gccgacacc cccggccgg cagtgctac gagccccac 
acccagccc ctttcgtgc aatacggtc gttgcgagt 
tgggcgcga ggctctgaa ggttttagt ttcccaccc 
gaagccggt tatccccta gaacccgcc acatcccgc 
tactacttg ctggaaacg cactacatc agccccaca 
gatggcagt ttggggtct ctcgacggg tccggcttg 
aggctgtat tataccccc gaattacgc cggcacgac 
gctggcgtt atatcaata gggatggat ccgaactgg 
agacatata ataccgcca ggacaacaa agagttgtc 
tcttcagga cattgcgtg tcggagtgc accaaacaa 
gctttcccc cataatggc ataaacatg ttcgctgtt 
gttatgaaa acacaccga attggacga caggttgct 
ttgaagcac atcagaggc aatgtggag cagcctccc 
attgcggcg gatgacaat ctcgatgct gattaccag 
gagtatcgc aggctcgga attcccgtc aaaatacta 
cccggcgac catcttatt gccgagtgt acttacaac 
agctcgggc cggacggcc atcacccta ggaggactc 
acaagtcgc gaggaaacc tgcctggtt atggggctc 
tattacccg aaacagcgc tctttagcg gcatgtcac 
agtttgccg agcctgccg acagtcctg cattctttg 
ggaatacaa gaactgagc ccggggtcg aaccccgtg 
aggatagcc gcccctcca gagttggcc gggatgact 
ctggaatcc aggttggtg tcttatgac tgggacaat 
catttccac aattttcaa gaagcaacg atgaaaggt 
tccttcaag ccattgtgc cggactgcg caatcgacg 
atattgccg ggcacagat aaggaattc cattacccg 
aatattacg atagcatac gtccgtaac aatgcagac 
caatgtaac agctacaaa aggtatagc acagaaggc 
tggccggga ggaacgccc gtcatggaa ttggaaggt 
aatcatata gaaggagca gctcgtagc aaagtttcg 
cgtagcagc agcagtcca accgctgaa gaatcgctt 
ggattgagt agagtgaac tctgcttgt agatgttat 
tccttccag actgtgtta gtgctctgt gttttgttg 
gtttggtcc aaccactga
    mRNA sequence
    • >TC014330 RA
      taggcagag aaacgtttc agcagtctg aaataaacc 
gtgatagag ggagtatga gtcgctcgc ttcgctatt 
ccgtcaatt ccgggtgca tattcctaa cactatcaa 
agtaaaaat gcgtcggac ggctaatga agctacgtg 
ataagtgaa taatcaatg attcgtgtt tgagttgga 
aagttcctc gggtttcgg gagctttgc ccggagttt 
gtttgtgtt tttcaccgt tgtatggaa atcgtgacc 
gctttgtgt tccggaaac attctttgt gacttttca 
ggcaatatt tggattatc tctcgtggt ttttatatg 
aagcctccg tgcaactgc taacaatgg tccctaaat 
tagttcaag tgctaagtg ttttgtgtg ttgtgcaat 
acggaaaag cggctagat ggtcacact ctgcaatgc 
tcagcccgg actatcgac ttctttggt cgttagggc 
cgggacctc aagacatca cgtttgaat tgcaagtga 
ggactctgg gatatgttg ggtttggct tttcaaagg 
atggacgca tgacagggg ctgacatgg tcgtcggat 
gggtggaca aaggacaag tctacttcc aggatcgcc 
atgttaagg attctcctg taagttctc gggatagag 
aaccggaag tggatccaa gccaggatt atcaacttc 
tgttgggtt atgagaacc acacacata ctgtgttac 
gattcagga ggaaacttg atacttgtg accatcatg 
acatcccca tcacgaacg atacgatga gggtagtgt 
gggctttcc actcggagg agcctgtgg ggggatcag 
tgggtccaa actcattgc cacaacatg acgtcgaat 
tcagaggaa cccagtcgc tttatttgg ttcagaggg 
ccgatcagg aggctcctg ggcctgaag aaacggcaa 
gaatttggg aactgcgga acccggcgg tggaaccgc 
cagcacctg ctgaaaact tgtactggt gccgagttt 
tcaaaattc ctgtcatta ccaagaagc accacttaa 
ttcggtacg agccacttc agggaatca gaggcccga 
ccggattgc agcacgtcg tgctgtacg agtgtcagg 
acagccccc aggtcgcgc agctggccg acacccccg 
gccggcagt gctacgagc cccacaccc agccccttt 
cgtgcaata cggtcgttg cgagttggg cgcgaggct 
ctgaaggtt ttagtttcc cacccgaag ccggttatc 
ccctagaac ccgccacat cccgctact acttgctgg 
aaacgcact acatcagcc ccacagatg gcagtttgg 
ggtctctcg acgggtccg gcttgaggc tgtattata 
cccccgaat tacgccggc acgacgctg gcgttatat 
caataggga tggatccga actggagac atataatac 
cgccaggac aacaaagag ttgtctctt caggacatt 
gcgtgtcgg agtgcacca aacaagctt tcccccata 
atggcataa acatgttcg ctgttgtta tgaaaacac 
accgaattg gacgacagg ttgctttga agcacatca 
gaggcaatg tggagcagc ctcccattg cggcggatg 
acaatctcg atgctgatt accaggagt atcgcaggc 
tcggaattc ccgtcaaaa tactacccg gcgaccatc 
ttattgccg agtgtactt acaacagct cgggccgga 
cggccatca ccctaggag gactcacaa gtcgcgagg 
aaacctgcc tggttatgg ggctctatt acccgaaac 
agcgctctt tagcggcat gtcacagtt tgccgagcc 
tgccgacag tcctgcatt ctttgggaa tacaagaac 
tgagcccgg ggtcgaacc ccgtgagga tagccgccc 
ctccagagt tggccggga tgactctgg aatccaggt 
tggtgtctt atgactggg acaatcatt tccacaatt 
ttcaagaag caacgatga aaggttcct tcaagccat 
tgtgccgga ctgcgcaat cgacgatat tgccgggca 
cagataagg aattccatt acccgaata ttacgatag 
catacgtcc gtaacaatg cagaccaat gtaacagct 
acaaaaggt atagcacag aaggctggc cgggaggaa 
cgcccgtca tggaattgg aaggtaatc atatagaag 
gagcagctc gtagcaaag tttcgcgta gcagcagca 
gtccaaccg ctgaagaat cgcttggat tgagtagag 
tgaactctg cttgtagat gttattcct tccagactg 
tgttagtgc tctgtgttt tgttggttt ggtccaacc 
actgaccta gtgatttaa cttagtgac ctcatgact 
gatttttat attcctgaa tcgtacatg gtgatagca 
acgactgct gaattttta tttattgac attgttgtg 
aattatttg agtcataca tttactagt ccgttcggc 
cagatcata cgtggtttt tgaatgatt tctctcttt 
ttctataaa tgtatttag taaagcgac taatccaag 
tgcaatagt ttccggata cggaaaaat cgctttgct 
ggaaaatat gtagcggta gtggatgcc gaatgggac 
agttgaata cgccgcgac aggtctgtg atgaaagta 
gtaataagc acttttcat gtttctttt ctttaggta 
atgacgtct cggttttgc tactgttgt tagtttact 
cttctttaa cccgtcacg agggttgta tttgtattt 
atatgtaaa tttaatatc ctgtgtata tttgtagtc 
tcgactggt gctatattt tttattctt ttgtatgtt 
atttaattt cgactttcc ttagtacga taaatttct 
tgtacagtg tagataact tgtgtatat tttataaat 
aaacattaa taaacaaaa tctagcaat
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