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Gene information
General information
TC014328 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9123501..9130401
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014328
    iBeetle screen iB_05609

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    about 50

    head partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014328 RA
      MESRGNVLF VFALLMLTT LVSARAVNI SNTWMLPEE 
      GFPVFYRYF RDRISWYEA DAVCQFHHS NLVTADSTS 
      QYDAIRAYL KELDINENV WIGLSKTTG RPNFTWTNF 
      RPLSGEGHW QQQLPKSDD SLCTAMDPV ADFLWKPMN 
      CGGPEIASF ICELPVPSW AMGPKGCLL TELPSLTVL 
      YIPEQSALE LTSDCGLDG TKRIACKGN ADREDILKQ 
      LSCSISPED FEDKPTKNP ILPNITSSS VSETSEDVV 
      SSKTTKPWT SNTIDIDYG MPTRHRRET EDTLSPVST 
      KSTTDLSNQ EAHKQEIIT MSSQSLETE TTSSNPDGD 
      VTLDASVSL KSDKSTELK TTQVTDLTT QDINAQTVT 
      AEPKTANPE SGDVGEYPA AINQGQLFS IIENGTMFD 
      ILEVNDTSE DVKFAKEIV METPQLETG TSLKYNSEN 
      SENFYTTVV YHKEPPSEA SFVTNKSVK KPDKHSKKD 
      MLKEKDPKK ANHDNKYLP KNLNTLEDT TENEKTLLK 
      EVEMFPLLT DPLTKLNRT YRKDIPVKA DKHHHVEVA 
      WGEKENTKK LFITTKRTE ALQTSTPSA KENLELLNS 
      SEEEVMHKI DSKIDLLNN NSAINNESE TVTLPKPEK 
      AIEDSDLMK KQFLPKSLF GETKPGTET ATSEPLEPE 
      PQAQPRPNR QRQLTRPQR RSFYPYFFS RVLG
    CDS sequence
    • >TC014328 RA
      atggagtcc cgtggaaat gtgttgttt gtgttcgcg 
      ctgctaatg ttaacgaca ttggtttct gcgcgagct 
      gtgaacatt agcaacacg tggatgctt ccagaggaa 
      ggttttccg gtattttat cgttatttc cgggatcgc 
      atatcttgg tacgaagcc gacgccgta tgccagttt 
      catcactcc aatctggtt accgctgac agtacatcg 
      caatacgat gccatacga gcgtatctc aaagaatta 
      gacattaac gagaatgta tggattgga ctgtctaag 
      accacaggg cgaccaaat ttcacctgg acaaatttt 
      cgtcctttg tccggtgag ggtcactgg cagcaacag 
      ctacccaaa agtgacgat tctctttgt actgccatg 
      gatccagtc gcagatttc ctgtggaaa ccgatgaac 
      tgtggagga cccgaaata gcgtccttt atttgtgaa 
      ttacccgtt ccatcgtgg gctatgggt cccaaagga 
      tgtcttttg acagaacta ccgtcttta acagtttta 
      tacattccg gaacagtcg gcacttgaa ctcacttca 
      gactgtggt cttgacggc acaaaaaga attgcgtgc 
      aaaggaaac gcggaccgc gaagacatc ctaaaacaa 
      ctgtcgtgc tcgatatcc cccgaagat ttcgaggat 
      aagccgaca aaaaatcca attttgccc aacataaca 
      tcaagcagc gtatctgaa acatccgag gatgttgtc 
      tctagtaaa acgacaaaa ccgtggact tcgaatacc 
      atcgacatc gattatgga atgccgaca cgtcatcgc 
      agagaaacc gaagacaca ctgagtcct gtttccacc 
      aaaagtacg actgattta tccaatcaa gaagcgcac 
      aaacaagaa atcattacg atgtctagt cagagcctc 
      gaaactgaa acaaccagt agcaatcct gatggagat 
      gtgactcta gacgcgagt gtatctttg aaatccgac 
      aaatccaca gagcttaaa acgacacaa gtaaccgat 
      ttaaccact caggacatt aatgctcag actgttact 
      gctgaaccg aaaacagcg aatccagaa agtggagat 
      gtgggcgag tatcctgct gctattaat caaggacag 
      cttttcagt attatcgaa aacggaaca atgttcgat 
      attctggaa gtcaacgat acgtctgag gacgtgaag 
      tttgccaaa gaaatagtg atggagaca ccacagcta 
      gaaactgga acgtctttg aaatataat agcgaaaat 
      agtgagaat ttttacacg acggtggtt taccacaaa 
      gagcccccg tccgaggcc agctttgtc acaaacaaa 
      tcagttaaa aaacccgat aaacattcg aaaaaagac 
      atgttgaag gaaaaagat ccgaaaaag gcaaaccac 
      gataataaa tatctcccg aaaaatttg aatacgttg 
      gaagacacc acagaaaac gaaaaaact ctcttaaaa 
      gaagtggaa atgttcccg ctcctgacg gaccccctg 
      acgaaactt aaccgaact tacaggaaa gacatcccg 
      gttaaagcc gacaaacac caccacgtc gaagtcgcg 
      tggggcgag aaagaaaac acgaagaaa ttgtttatc 
      accactaaa cgaaccgag gccttacag acctcaaca 
      ccatccgcg aaagaaaac ttggaactt ctcaacagc 
      agcgaagag gaagttatg cacaagatt gactcaaaa 
      atagatcta ctcaacaac aactccgcg ataaacaac 
      gagagtgaa actgtaaca ctccccaag ccggaaaaa 
      gcaattgaa gattccgat ttgatgaaa aagcagttt 
      ttgccgaaa tcgcttttt ggtgaaacc aaaccgggc 
      acagaaacc gcaacgagt gagcccttg gagcctgaa 
      cctcaggct cagccgagg cccaatcgc caaagacag 
      ctcacccgg ccacaaaga aggtcgttt tatccgtat 
      tttttcagt agggtttta ggttga
    mRNA sequence
    • >TC014328 RA
      tggcttctg aagcttcag ttttcagtc gtgcatgcg 
      agggccagg ttagaatgg agtcccgtg gaaatgtgt 
      tgtttgtgt tcgcgctgc taatgttaa cgacattgg 
      tttctgcgc gagctgtga acattagca acacgtgga 
      tgcttccag aggaaggtt ttccggtat tttatcgtt 
      atttccggg atcgcatat cttggtacg aagccgacg 
      ccgtatgcc agtttcatc actccaatc tggttaccg 
      ctgacagta catcgcaat acgatgcca tacgagcgt 
      atctcaaag aattagaca ttaacgaga atgtatgga 
      ttggactgt ctaagacca cagggcgac caaatttca 
      cctggacaa attttcgtc ctttgtccg gtgagggtc 
      actggcagc aacagctac ccaaaagtg acgattctc 
      tttgtactg ccatggatc cagtcgcag atttcctgt 
      ggaaaccga tgaactgtg gaggacccg aaatagcgt 
      cctttattt gtgaattac ccgttccat cgtgggcta 
      tgggtccca aaggatgtc ttttgacag aactaccgt 
      ctttaacag ttttataca ttccggaac agtcggcac 
      ttgaactca cttcagact gtggtcttg acggcacaa 
      aaagaattg cgtgcaaag gaaacgcgg accgcgaag 
      acatcctaa aacaactgt cgtgctcga tatcccccg 
      aagatttcg aggataagc cgacaaaaa atccaattt 
      tgcccaaca taacatcaa gcagcgtat ctgaaacat 
      ccgaggatg ttgtctcta gtaaaacga caaaaccgt 
      ggacttcga ataccatcg acatcgatt atggaatgc 
      cgacacgtc atcgcagag aaaccgaag acacactga 
      gtcctgttt ccaccaaaa gtacgactg atttatcca 
      atcaagaag cgcacaaac aagaaatca ttacgatgt 
      ctagtcaga gcctcgaaa ctgaaacaa ccagtagca 
      atcctgatg gagatgtga ctctagacg cgagtgtat 
      ctttgaaat ccgacaaat ccacagagc ttaaaacga 
      cacaagtaa ccgatttaa ccactcagg acattaatg 
      ctcagactg ttactgctg aaccgaaaa cagcgaatc 
      cagaaagtg gagatgtgg gcgagtatc ctgctgcta 
      ttaatcaag gacagcttt tcagtatta tcgaaaacg 
      gaacaatgt tcgatattc tggaagtca acgatacgt 
      ctgaggacg tgaagtttg ccaaagaaa tagtgatgg 
      agacaccac agctagaaa ctggaacgt ctttgaaat 
      ataatagcg aaaatagtg agaattttt acacgacgg 
      tggtttacc acaaagagc ccccgtccg aggccagct 
      ttgtcacaa acaaatcag ttaaaaaac ccgataaac 
      attcgaaaa aagacatgt tgaaggaaa aagatccga 
      aaaaggcaa accacgata ataaatatc tcccgaaaa 
      atttgaata cgttggaag acaccacag aaaacgaaa 
      aaactctct taaaagaag tggaaatgt tcccgctcc 
      tgacggacc ccctgacga aacttaacc gaacttaca 
      ggaaagaca tcccggtta aagccgaca aacaccacc 
      acgtcgaag tcgcgtggg gcgagaaag aaaacacga 
      agaaattgt ttatcacca ctaaacgaa ccgaggcct 
      tacagacct caacaccat ccgcgaaag aaaacttgg 
      aacttctca acagcagcg aagaggaag ttatgcaca 
      agattgact caaaaatag atctactca acaacaact 
      ccgcgataa acaacgaga gtgaaactg taacactcc 
      ccaagccgg aaaaagcaa ttgaagatt ccgatttga 
      tgaaaaagc agtttttgc cgaaatcgc tttttggtg 
      aaaccaaac cgggcacag aaaccgcaa cgagtgagc 
      ccttggagc ctgaacctc aggctcagc cgaggccca 
      atcgccaaa gacagctca cccggccac aaagaaggt 
      cgttttatc cgtattttt tcagtaggg ttttaggtt 
      gagtttagt atgttaaaa aaagagcgt gatttttga 
      aagcacttt aatggttgt tatttaaga ttgttaaag 
      tcgagtgtt aaatcggaa aaaatgttt agttgcact 
      gaaattgtt cgatcgtgt tgcattcgc tacaacgat 
      aactgttta ttactgtta cttttttaa ttcttatca 
      tagattgta gtttaaaaa caaatattt ttgttgttg 
      taataatag ttctttgtt ctttagttg taaaaataa 
      gtatagcat aggactgtt tttatagga ctagcgttt 
      tgtggaatg aataagttt tacaacact tttggtaaa 
      aagctgtac aagctatgc accgtcctg cttcccgtc 
      ctacgcccc caaacatgc tgataaacg aaacacatt 
      gtcactgct ttaaaagca aaatatttt gcacacgat 
      gtaaaaaca tttcaatgt acataatta tgtagttta 
      attgtgtta ataaaaaaa atcgtgata a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY