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Gene information
General information
TC014298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8681697..8687095
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014298
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05606

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014298 RA
      MSSRSKEKV TAMFRKIFK SSNKDGEGP SKANPSGSG 
      SPARRLLRG CRDSVSPAA PQASPAFTS SKGKKGEDG 
      PSHKSVRAR RLMKEYRDL QRLQNSKTD PVFTVELVD 
      DNLFEWHVK VYKLDAESE LGNDMKELG INYILLHVV 
      FPENFPFAP PFMRVISPR IEKGFVMEG GAICMELLT 
      PRGWASAYT VEAVIMQFA ASVVKGQGR IQRKTKGQK 
      VFSKRTAEE SFRSLVKTH DKYGWVTPS LADG
    CDS sequence
    • >TC014298 RA
      atgagcagc cgatcgaag gaaaaagtc accgcaatg 
      ttccgcaaa attttcaaa tcctccaac aaagacggc 
      gagggtccc agcaaggcc aatccctcg gggtctggt 
      tctcccgcg cgccgcctc ctgaggggt tgccgcgat 
      tccgtgtct cccgccgcc ccccaagcc agtccagcc 
      ttcacctcc agcaagggg aaaaagggg gaagacggc 
      ccctcgcac aaaagtgtc cgcgcccgg agactcatg 
      aaggagtat cgcgatttg cagcgcctc caaaactcc 
      aaaacggac ccggtgttt accgtcgag ttagttgac 
      gataatttg ttcgagtgg cacgtgaaa gtgtacaaa 
      ctggacgct gaaagtgaa ctaggcaac gacatgaag 
      gagttgggg atcaactac attctgctg catgttgtt 
      tttcccgag aactttccc ttcgcccct cccttcatg 
      cgggtgatt tcgccgcgg atagagaaa gggtttgtt 
      atggagggc ggcgcgatt tgcatggag ttattgaca 
      ccaaggggg tgggcgagt gcttacaca gtcgaggcc 
      gttatcatg caattcgcc gccagtgtc gttaagggt 
      cagggccgc atccagagg aagaccaaa gggcaaaaa 
      gtgttcagt aaaaggacg gcagaggag agctttagg 
      tcgctggtg aagacccac gacaagtac ggctgggtc 
      acacccagc ctagccgat ggttag
    mRNA sequence
    • >TC014298 RA
      tcgcatcga tattaagaa atcacaaac acaaaaatg 
      cgccttcca ggggctgca aacacccac acccacacc 
      aactcaatt atcaaagtg ctgcatgca aacttaaca 
      ctggcgacg ccaacaaat gtgtcgttt ccaattgta 
      ataaagccg ttaatgtcc gcaaagtgt gcttaacgt 
      aatcagcaa gcccagctg acgaggctg tcttttgag 
      ccatcttgg acgccggtt ttgtgaggc gccggccat 
      cgatccgac ttagtgcgc caaatcaag tggcctgtg 
      catcatcgc tgccagttt atcagcaag ggtcagcta 
      gagcctgta ccaacatct tgcttatct cagttttat 
      caatggaga agtggactg ttttggggc ttacagcag 
      ttttgcagc cgtagtcgc ggatttgcg tcgtaaaaa 
      tcgccaaaa tgagcagcc gatcgaagg aaaaagtca 
      ccgcaatgt tccgcaaaa ttttcaaat cctccaaca 
      aagacggcg agggtccca gcaaggcca atccctcgg 
      ggtctggtt ctcccgcgc gccgcctcc tgaggggtt 
      gccgcgatt ccgtgtctc ccgccgccc cccaagcca 
      gtccagcct tcacctcca gcaagggga aaaaggggg 
      aagacggcc cctcgcaca aaagtgtcc gcgcccgga 
      gactcatga aggagtatc gcgatttgc agcgcctcc 
      aaaactcca aaacggacc cggtgttta ccgtcgagt 
      tagttgacg ataatttgt tcgagtggc acgtgaaag 
      tgtacaaac tggacgctg aaagtgaac taggcaacg 
      acatgaagg agttgggga tcaactaca ttctgctgc 
      atgttgttt ttcccgaga actttccct tcgcccctc 
      ccttcatgc gggtgattt cgccgcgga tagagaaag 
      ggtttgtta tggagggcg gcgcgattt gcatggagt 
      tattgacac caagggggt gggcgagtg cttacacag 
      tcgaggccg ttatcatgc aattcgccg ccagtgtcg 
      ttaagggtc agggccgca tccagagga agaccaaag 
      ggcaaaaag tgttcagta aaaggacgg cagaggaga 
      gctttaggt cgctggtga agacccacg acaagtacg 
      gctgggtca cacccagcc tagccgatg gttagatag 
      tcttgtcca aaaatatcg aatccgttc cgtttaaca 
      gtgttatct gaatgttca gaatagata tgtatttta 
      tgataaagc tgtatactt atattgtcc agtttagac 
      atttattca aatcacggt taggtaatc atgattcgt 
      tcctttcgc caaagaaac ggaaaatcc ttccaatat 
      tctgatccc gatttaatt ggaattccc aagtagtgt 
      ttgtcacat ttgactttt atcttccct tgagtttcc 
      cttgcgatt tcgccccag tgcatgcga gtaatatct 
      atcagtgtg gaacaaaca tcaaaacta taaagcacc 
      aatttcaag ctcattttt tcgataata tgtgttgaa 
      gcttcgcga aaataatgt tagacaagt tgctgtacc 
      cacaattac gttcaatgt agataggaa atttttaaa 
      ttcaatact ttttacccg tacttgtat cttactaaa 
      cttgtgtca ttttcatta tgagtattg aaaatgagg 
      cgagtttag aaatggcta aatggtaat gtgttttgc 
      atgactgaa atttttcat ttgaaaaaa aatgtgatt 
      gtttactat acatgacac aaataatac agggtgaac 
      aaaaggtga tgctaatac tttaggcaa atttgattg 
      ctcgctttg ttcagcctg tataatctt taattttgt 
      tttgtcaag ttatgcggc aaaactgaa gtactaaag 
      gctaatgtt caaaaatga aaatgctca tacaaagta 
      tattttata gtacattta agtaaaaca ggtgagtta 
      gtataattg ttgtacctc caaaaagtc cactgaaac 
      agtattttt agctaatag ttgtgattt gttattaaa 
      tgtgtattt ttttattta ctattttgt cttccaggg 
      tcaatttta tgtaaccaa tttattttt aagatacat 
      atttaaata tgagtcaag taattgtta cttaatgat 
      aataaatgt aacattaaa tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY