Status
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Gene information
General information
TC014296 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8576441..8601622
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014296
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05605

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014296 RA
      MEDNCTMRP CATDFSIAA IMSRHGRAP RTRCRERDP 
      PDSISPLEK FVESSASSV PSTPEPVGL DCSLVSPDI 
      ESGRESPVD VSSTSESGV PSHSIANGR RSPAAARNP 
      LLQERWTSE ELRTVTCHL ETKDLWEKF NELGTEMII 
      TKSGRRMFP TVRVSFTGI RPDQRYAVL LDIVPVDNK 
      RYRYAYHRS SWLVAGKAD PPAPCRMCA HPDSPFTGE 
      QLRKQVVSF EKVKLTNNE MDKHGHLVL NSMHKYQPR 
      IHLVKRPDG ASGQIVDLE NEEYKTFIF PETVFTAVT 
      AYQNQLITK LKIDSNPFA KGFRDSSRL TEFERETME 
      SMLAEQHYL RSPLRPFAD LDTHNNNLS IEEKAILAA 
      RSQLFLRAA AAAAGPYGP LMGGLYGAG ASQSSAVPP 
      GVLWNQWAC LGPSLLAAQ HQHQLAVAA AGSSHLNSQ 
      MSPLARPLA QHRYSPYAS LPKSSPDTS LRDGRESPS 
      SPT
    CDS sequence
    • >TC014296 RA
      atggaggat aactgcacc atgaggccc tgcgccacg 
      gactttagc attgctgct atcatgtcg aggcatggc 
      agggcgccc cggactaga tgcagggaa cgcgatccg 
      ccggattcc atctcgccg cttgaaaaa tttgtcgag 
      tcctccgcc tcgtcagta ccttcgacc ccggagccc 
      gtgggctta gattgttcg ttagttagt ccggacatt 
      gaaagtggt cgtgaatca ccggttgat gtgtcttcc 
      acgtccgaa tcgggggtg ccatcacat tctattgcg 
      aatggtcgt agatcacct gcggctgcg agaaatccg 
      cttctgcag gaacgctgg accagcgaa gagctccga 
      acagttact tgtcattta gaaacaaag gatctctgg 
      gaaaagttc aacgaattg ggaacggag atgattatc 
      accaaatca ggacgccgc atgtttcct acggtccgg 
      gtttctttc accggaatt cgcccggac cagcgctac 
      gccgtgctg ctggacatc gtccctgtg gacaacaag 
      cgttaccgg tacgcctac catcggtct tcgtggcta 
      gtggccgga aaagcggac cctccagcg ccttgcagg 
      atgtgcgcc cacccggac tctcccttc accggtgag 
      cagctccga aagcaagtg gtttcgttc gaaaaagtg 
      aagctcacc aacaacgaa atggacaag catggacat 
      ttagtcctg aattcgatg cacaaatac cagccgagg 
      attcactta gtgaaacga ccggatggg gcttcagga 
      caaatagtc gacttggag aatgaggaa tataaaacg 
      tttatattt cctgaaact gtttttacg gctgtcacg 
      gcttatcag aatcagttg ataaccaag ctcaagatt 
      gacagcaac ccgttcgcc aagggcttc agagactcc 
      tcgaggctc accgagttc gaaagagag accatggag 
      tcgatgctc gcggagcag cactacctg cgctctccg 
      ctgcggcct ttcgccgac ttggacacc cacaacaac 
      aatttgtcg atcgaggag aaggcgatt ctggccgcc 
      aggtcgcag ttgtttctg agggcggcg gcggcggcg 
      gcgggccct tacgggccc ctaatgggc ggactctac 
      ggtgctggt gcttcgcaa agtagtgcc gttcctccg 
      ggggttttg tggaaccag tgggcttgc ttggggcct 
      tcgcttctg gcggctcaa catcaacac caattggcg 
      gttgccgcc gccggaagt tcgcatttg aattcgcaa 
      atgtcgcca ctggcgagg cctttggct caacaccgg 
      tactctccc tatgcttct cttcccaaa tcctcgccg 
      gatacgagt ctcagagac gggcgggag tcgcccagc 
      agcccgact tga
    mRNA sequence
    • >TC014296 RA
      cggttcttc aatacggaa aacattatt tgcaattac 
      ctacaaacg caattgttt tctccggtc gagtaataa 
      ctatttaat aattcgatt tctaacttc aacaaatga 
      ataagattg ttaaataaa gtgtagtgc taataaccc 
      agtaattaa ttacttatt gtttaaact aatagataa 
      ttatgactc tatgtagaa gtacctacc ttttccaag 
      cttgtgtta ggtataggt gtttaatgt gcgattttc 
      gtgacttgg tggaatcta ttaggtaaa atgaaaaaa 
      gtgctaaaa gtgtttgaa gtgaatgtg tttgaagat 
      gttgacgtt tcgtaagcg ttgaaacct ttgacctag 
      tgggtagta atagcgggt gaaaatgac gagaaaata 
      attttcgtt agtccctaa agtagcgta ggtaagcat 
      catcagaag ggttcacca aaacaatcg gccaggttc 
      gctgatagg cgtcgccag gtcgtaagc gcctccctg 
      gggcgtgtc atcttgctt cccaccccg cggaattcg 
      tctgatcaa gcagcagag aagaagaaa ggcccggat 
      agtgcaggt tctacagcc cgcccaggt gcagccgcg 
      atcggggag gtaaaaaca aagccggtc gcgagttgt 
      catcgacga ggacagagg ggagaatcg tcgaaaaaa 
      tggcacatt cctttgata acgtctcga cggagtggt 
      cagtcggcg ttgaacagc gtcgctgta gacaacgga 
      gcgccgttg ataatcaat tacagcaag caaagtgag 
      gaacgcctc ttgaagtgg gagcagcca atctgaatg 
      ctttcgctg catatcaaa gtaatcttg ttaaatgtg 
      agtcaaaag gatctaaat attcattct gtcaaaaca 
      gcttgcttc tgtgccggc gcgaaaact gcctcacac 
      ctagtgaaa gtacacgga agtgcctaa aatgacatg 
      tagacggtc acgccgccc ccggtcgta gcggctcat 
      tcacgtgct ttagtttac tggttttgt gacttgacc 
      tgacctgac ctgcacggt ggtgatgtg gtcgacgag 
      gtgatggag gataactgc accatgagg ccctgcgcc 
      acggacttt agcattgct gctatcatg tcgaggcat 
      ggcagggcg ccccggact agatgcagg gaacgcgat 
      ccgccggat tccatctcg ccgcttgaa aaatttgtc 
      gagtcctcc gcctcgtca gtaccttcg accccggag 
      cccgtgggc ttagattgt tcgttagtt agtccggac 
      attgaaagt ggtcgtgaa tcaccggtt gatgtgtct 
      tccacgtcc gaatcgggg gtgccatca cattctatt 
      gcgaatggt cgtagatca cctgcggct gcgagaaat 
      ccgcttctg caggaacgc tggaccagc gaagagctc 
      cgaacagtt acttgtcat ttagaaaca aaggatctc 
      tgggaaaag ttcaacgaa ttgggaacg gagatgatt 
      atcaccaaa tcaggacgc cgcatgttt cctacggtc 
      cgggtttct ttcaccgga attcgcccg gaccagcgc 
      tacgccgtg ctgctggac atcgtccct gtggacaac 
      aagcgttac cggtacgcc taccatcgg tcttcgtgg 
      ctagtggcc ggaaaagcg gaccctcca gcgccttgc 
      aggatgtgc gcccacccg gactctccc ttcaccggt 
      gagcagctc cgaaagcaa gtggtttcg ttcgaaaaa 
      gtgaagctc accaacaac gaaatggac aagcatgga 
      catttagtc ctgaattcg atgcacaaa taccagccg 
      aggattcac ttagtgaaa cgaccggat ggggcttca 
      ggacaaata gtcgacttg gagaatgag gaatataaa 
      acgtttata tttcctgaa actgttttt acggctgtc 
      acggcttat cagaatcag ttgataacc aagctcaag 
      attgacagc aacccgttc gccaagggc ttcagagac 
      tcctcgagg ctcaccgag ttcgaaaga gagaccatg 
      gagtcgatg ctcgcggag cagcactac ctgcgctct 
      ccgctgcgg cctttcgcc gacttggac acccacaac 
      aacaatttg tcgatcgag gagaaggcg attctggcc 
      gccaggtcg cagttgttt ctgagggcg gcggcggcg 
      gcggcgggc ccttacggg cccctaatg ggcggactc 
      tacggtgct ggtgcttcg caaagtagt gccgttcct 
      ccgggggtt ttgtggaac cagtgggct tgcttgggg 
      ccttcgctt ctggcggct caacatcaa caccaattg 
      gcggttgcc gccgccgga agttcgcat ttgaattcg 
      caaatgtcg ccactggcg aggcctttg gctcaacac 
      cggtactct ccctatgct tctcttccc aaatcctcg 
      ccggatacg agtctcaga gacgggcgg gagtcgccc 
      agcagcccg acttgagca ggttccttc atcttgtga 
      tatatagaa aatgtgtat aaagctgta aactctgac 
      tcacactgt aaaaacaat aaagacaac aatttgcgt 
      tattttata ttaagtatt agcggcagt atggttaag 
      ttgttgcaa taacggtta agtggcgtt gctactatt 
      ttgtaaata gtcgcttga ggcgcaaaa ccacacttg 
      tgtagtgta aaatcttga gtgtgttaa ctgattctg 
      ctaaagttc caaacaaat gtataaaat gtctgtaaa 
      taagtataa tttattata gtgaactaa tgtgacttt 
      tgttcatta tgtctttca gcggaaaaa gcttcattt 
      attagacgt ttttcacaa aatcgcgct gaaggacac 
      gatgactga ctcgtagat cgttaaact ccctcgcat 
      aattaattg tttaaaagc tgcttaatg taattgcct 
      agttgtgta tgacacagt cgaataggt ttgattatt 
      atattctaa atcttttca ttgaaataa aagcatttt 
      aagaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY