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Gene information
General information
TC014294 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8529115..8533875
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014294
    iBeetle screen iB_05604

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014294 RA
      MANINLDKE TFHRRLKKL YSAWQKSES ENGFSKMDA 
      LVTAVGVDD EVVYSKSGA LQTWLLGYE LTDTIMVLT 
      EKKAHFLAS KKKIDFLRQ AETKDENSI QLSLHVRDR 
      TSDEANFKL LIDAIKESK NGKTIGVFS KDNYPGAFM 
      DAWRAALSK VSFQTVDVS AAIAYLLSP KEDSEIITI 
      KKACMVSVD VFTKYLKDQ IMEIIDSDR KVKHSKLAE 
      GVESAIADK KYVSGVDVS QVDMCYPAI IQSGGNYSL 
      KFSVVSDKN TLHFGAIIC SLGARYKSY CSNIVRTLL 
      VNPNEQVQN NYNFLVTLE EEVLKKLQA GTKLSEVYE 
      AGYNFVKKE KPELADNLT KNFGFATGI EFKESSLMI 
      GPKTTLPAK KGMVFNVNM GFSNLENKD ATDKEGKTY 
      ALFIGDTVM VNEGQPASV LTMSKKKIK NIGIFLKDE 
      SDDEDNDDE KENAPKPEI LGRGKRTAV LESKLRTEH 
      TSEEKRKEH QKELASMLN EKAKERLAK QSGSKDVEK 
      VRKSTVSYK NVNQMPRVP EVKELKLYV DQKYETVIL 
      PIYGIAVPF HISTIKNIS QSVEGDYTY LRINFFHPG 
      STMGRTDGG NYQQPEATF VKEVTYRSL NTKEPGEIS 
      PPSSNLNTA FRLIKEVQR KFKTREAEE REKEDLVKQ 
      DTLVLSQNK GNPKLKDLY IRPNIVTKR MTGALEAHS 
      NGFRYTSVR GDKVDILYN NIKNAFFQP CDGEMIILL 
      HFHLKHAIM FGKKKHVDV QFYTEVGEI TTDLGKHQH 
      MHDRDDLAA EQSERELRH KLKTAFKSF CEKVEAMTK 
      QEIEFDTPF RELGFPGAP YRSTVLLQP TSGCLVNLT 
      EWPPFVITL EDVELVHFE RVQFHLKNF DMIFVFKDY 
      QRKTAMVNA IPMNMLDHV KEWLNSCDI RYSEGVQSL 
      NWVKIMKTI TDDPEGFFE SGGWTFLDP ESDEEAQAQ 
      EEETEDEDE VYEPTDMES VSEESEEDS EYSEGDTEE 
      SDESASGED DLGSDEESG KDWSDLERE AAEEDRERN 
      FDDTFDRKK SHHKSSKPS KHSSSKSNH KGSPSKHSN 
      NHHSSSSKH SSSKHSSRD SDRRKSSSS SKSKSSPSK 
      DKDRHHRSS HSDSKKRSR EESSDKHKS KKSKK
    CDS sequence
    • >TC014294 RA
      atggcaaac ataaatttg gacaaagaa acttttcac 
      cgccgctta aagaagttg tacagtgct tggcaaaaa 
      tcggagagc gagaatggt ttctctaag atggatgct 
      ttggttaca gccgtcggt gtggacgac gaagtggtt 
      tacagtaag tcgggggcc ctccagacc tggcttttg 
      ggctacgaa ctgaccgac accatcatg gtcctgacc 
      gagaagaaa gcccacttc ctggccagt aagaaaaaa 
      attgatttt ctgagacaa gccgaaact aaggacgag 
      aattcgatt cagttgtct ctgcatgtg cgagaccgc 
      acgagcgac gaggccaat ttcaagctc ctcattgac 
      gcgatcaaa gagagcaag aacgggaaa acaatcggg 
      gttttctcc aaagacaac tacccgggc gctttcatg 
      gacgcctgg cgcgcggct ttgtccaaa gttagcttc 
      cagacagtt gatgtgagc gcggctatt gcctatttg 
      ctatcgcca aaggaagat tcagaaatc ataacaatt 
      aaaaaagcc tgcatggtg agtgtcgat gtgtttacc 
      aagtatctc aaagaccag ataatggag ataattgac 
      tccgatcgc aaagtcaag cacagcaaa ctggcggaa 
      ggcgtggag tctgcaatc gccgacaaa aaatacgtt 
      tcgggcgtc gatgtttct caggttgat atgtgctat 
      cctgccata atccagtca gggggcaac tacagccta 
      aaattcagt gtagtgagt gacaagaac actctacac 
      tttggggcc atcatatgc tcgctgggc gcccggtac 
      aagtcctac tgttcaaac attgtccgc acgttgctt 
      gtgaaccca aacgaacaa gtacaaaac aattataat 
      ttcctcgtt acccttgaa gaagaggtt ttgaaaaaa 
      ttgcaagcc gggacaaag ctttcagag gtatacgaa 
      gccggttac aactttgtt aagaaagaa aagcctgaa 
      ctggcggat aatctgacc aaaaatttc ggtttcgca 
      acggggatt gaattcaaa gagagctcg cttatgatc 
      ggcccaaaa acaactctc ccggcgaaa aagggaatg 
      gtcttcaac gtcaacatg ggttttagc aacctggag 
      aacaaagat gcgaccgat aaagagggc aaaacgtac 
      gcgctgttt attggcgac actgtgatg gtgaatgaa 
      ggccaaccg gcctcggtc ctcaccatg tccaagaag 
      aaaatcaaa aatatcggg atctttctc aaggatgag 
      tccgatgat gaggataac gacgatgag aaagaaaat 
      gccccaaaa cctgaaata ctagggagg ggcaaacgc 
      accgccgtt ttggagtcc aaacttaga accgaacat 
      acgtctgaa gagaaacga aaagagcat cagaaggaa 
      ttggcttcg atgctgaac gagaaagca aaggaacgg 
      cttgctaaa caatcggga tcgaaggat gttgaaaaa 
      gttaggaaa agcacagtg tcgtataaa aatgtcaat 
      caaatgccc cgtgtgcct gaagtcaaa gaactgaaa 
      ctctacgtg gatcagaaa tatgaaact gtcattttg 
      ccgatttat ggaattgca gtgccgttc catatctcc 
      accattaag aatatatcg cagtcggtc gaaggcgat 
      tacacgtac cttcggatc aactttttc catcccgga 
      tccaccatg ggcaggacc gatggtggc aactaccag 
      cagcctgaa gccactttc gtcaaagaa gttacctac 
      cgcagtttg aacaccaaa gagcccgga gaaatttcc 
      cctccctct tcgaaccta aacactgca ttccgacta 
      atcaaggaa gtacaacga aaattcaaa acccgcgaa 
      gcggaagaa cgcgaaaaa gaagatctt gttaaacaa 
      gacacgctg gtcttgtcg cagaacaag ggcaatccc 
      aagcttaaa gatctttac atacgacct aatatcgtc 
      acaaagcgt atgaccggc gctttggaa gctcacagt 
      aacggtttc cggtacact tcagtcaga ggcgacaaa 
      gttgatatt ttatacaat aatattaag aatgcattc 
      tttcaacct tgcgacggt gaaatgatc atacttttg 
      cattttcac ctgaagcat gcgatcatg ttcgggaaa 
      aagaagcac gtggacgtg cagttttac acggaagta 
      ggtgaaatc acgactgat ttaggcaaa caccagcat 
      atgcatgat agggatgat ttggctgct gagcagtct 
      gagagggag ttgaggcac aagctgaag acggcgttc 
      aagagcttt tgcgaaaag gtcgaagcg atgacaaaa 
      caagaaatc gagttcgat acgccgttc cgggagctc 
      ggctttccc ggagccccc tacaggtcc acagtatta 
      ctccagccg acgtcgggt tgcctcgtc aatttaacc 
      gaatggccc ccgttcgta attacgctc gaagacgtc 
      gaattagtc cacttcgaa agagtccaa ttccatttg 
      aaaaacttc gatatgatt ttcgtcttc aaggactac 
      caacgcaaa accgcaatg gtgaacgcc atcccgatg 
      aatatgctg gaccatgtc aaagaatgg ctcaactct 
      tgcgacatc cgctactcc gaaggtgtc cagtcgtta 
      aactgggtc aaaatcatg aaaacaatc accgatgat 
      cccgaggga tttttcgaa agcggcgga tggacgttc 
      ctggacccg gaatcggac gaagaagcc caggcccaa 
      gaggaggaa acggaggac gaggacgaa gtttacgaa 
      ccgaccgac atggaaagc gtgtcggaa gaaagcgag 
      gaagattcc gaatactct gaaggcgac actgaggag 
      agtgacgaa agcgcttcc ggggaggac gatctagga 
      tctgatgaa gaatccggg aaggactgg tccgacctg 
      gagcgggaa gcagccgag gaggaccgg gaacgcaat 
      ttcgacgat actttcgac cggaaaaag tcgcatcac 
      aagtcgagc aaacccagc aaacactcg tcgagtaaa 
      agcaaccac aaaggctcg ccgagcaaa cattcaaac 
      aaccatcat tcctcaagt agcaaacat tcgagttcg 
      aaacatagc agcagagat agtgatagg cgtaaatct 
      tcttcaagt tcgaaatcg aaatcttca ccctcgaaa 
      gacaaggac cgccatcac agaagctcg cattcggat 
      tcgaaaaag cgcagtcgg gaggagagc agcgataaa 
      cacaagtcc aagaagtcg aagaagtaa
    mRNA sequence
    • >TC014294 RA
      cgtttcgaa ataatggtt tatttggtg ttggagtga 
      tggcagtct tgtgacatt ttgttgaca tttgcgagt 
      taccgctct tttgtgttt gcgcaaagt gcgcaaaat 
      aacgtctcg atctggcgc cttctcttc atgtaacgc 
      cgctgcgtt gttgtgtgg tgttaaatg tgtaaatat 
      cgcatgtaa caatcgaca atggcaaac ataaatttg 
      gacaaagaa acttttcac cgccgctta aagaagttg 
      tacagtgct tggcaaaaa tcggagagc gagaatggt 
      ttctctaag atggatgct ttggttaca gccgtcggt 
      gtggacgac gaagtggtt tacagtaag tcgggggcc 
      ctccagacc tggcttttg ggctacgaa ctgaccgac 
      accatcatg gtcctgacc gagaagaaa gcccacttc 
      ctggccagt aagaaaaaa attgatttt ctgagacaa 
      gccgaaact aaggacgag aattcgatt cagttgtct 
      ctgcatgtg cgagaccgc acgagcgac gaggccaat 
      ttcaagctc ctcattgac gcgatcaaa gagagcaag 
      aacgggaaa acaatcggg gttttctcc aaagacaac 
      tacccgggc gctttcatg gacgcctgg cgcgcggct 
      ttgtccaaa gttagcttc cagacagtt gatgtgagc 
      gcggctatt gcctatttg ctatcgcca aaggaagat 
      tcagaaatc ataacaatt aaaaaagcc tgcatggtg 
      agtgtcgat gtgtttacc aagtatctc aaagaccag 
      ataatggag ataattgac tccgatcgc aaagtcaag 
      cacagcaaa ctggcggaa ggcgtggag tctgcaatc 
      gccgacaaa aaatacgtt tcgggcgtc gatgtttct 
      caggttgat atgtgctat cctgccata atccagtca 
      gggggcaac tacagccta aaattcagt gtagtgagt 
      gacaagaac actctacac tttggggcc atcatatgc 
      tcgctgggc gcccggtac aagtcctac tgttcaaac 
      attgtccgc acgttgctt gtgaaccca aacgaacaa 
      gtacaaaac aattataat ttcctcgtt acccttgaa 
      gaagaggtt ttgaaaaaa ttgcaagcc gggacaaag 
      ctttcagag gtatacgaa gccggttac aactttgtt 
      aagaaagaa aagcctgaa ctggcggat aatctgacc 
      aaaaatttc ggtttcgca acggggatt gaattcaaa 
      gagagctcg cttatgatc ggcccaaaa acaactctc 
      ccggcgaaa aagggaatg gtcttcaac gtcaacatg 
      ggttttagc aacctggag aacaaagat gcgaccgat 
      aaagagggc aaaacgtac gcgctgttt attggcgac 
      actgtgatg gtgaatgaa ggccaaccg gcctcggtc 
      ctcaccatg tccaagaag aaaatcaaa aatatcggg 
      atctttctc aaggatgag tccgatgat gaggataac 
      gacgatgag aaagaaaat gccccaaaa cctgaaata 
      ctagggagg ggcaaacgc accgccgtt ttggagtcc 
      aaacttaga accgaacat acgtctgaa gagaaacga 
      aaagagcat cagaaggaa ttggcttcg atgctgaac 
      gagaaagca aaggaacgg cttgctaaa caatcggga 
      tcgaaggat gttgaaaaa gttaggaaa agcacagtg 
      tcgtataaa aatgtcaat caaatgccc cgtgtgcct 
      gaagtcaaa gaactgaaa ctctacgtg gatcagaaa 
      tatgaaact gtcattttg ccgatttat ggaattgca 
      gtgccgttc catatctcc accattaag aatatatcg 
      cagtcggtc gaaggcgat tacacgtac cttcggatc 
      aactttttc catcccgga tccaccatg ggcaggacc 
      gatggtggc aactaccag cagcctgaa gccactttc 
      gtcaaagaa gttacctac cgcagtttg aacaccaaa 
      gagcccgga gaaatttcc cctccctct tcgaaccta 
      aacactgca ttccgacta atcaaggaa gtacaacga 
      aaattcaaa acccgcgaa gcggaagaa cgcgaaaaa 
      gaagatctt gttaaacaa gacacgctg gtcttgtcg 
      cagaacaag ggcaatccc aagcttaaa gatctttac 
      atacgacct aatatcgtc acaaagcgt atgaccggc 
      gctttggaa gctcacagt aacggtttc cggtacact 
      tcagtcaga ggcgacaaa gttgatatt ttatacaat 
      aatattaag aatgcattc tttcaacct tgcgacggt 
      gaaatgatc atacttttg cattttcac ctgaagcat 
      gcgatcatg ttcgggaaa aagaagcac gtggacgtg 
      cagttttac acggaagta ggtgaaatc acgactgat 
      ttaggcaaa caccagcat atgcatgat agggatgat 
      ttggctgct gagcagtct gagagggag ttgaggcac 
      aagctgaag acggcgttc aagagcttt tgcgaaaag 
      gtcgaagcg atgacaaaa caagaaatc gagttcgat 
      acgccgttc cgggagctc ggctttccc ggagccccc 
      tacaggtcc acagtatta ctccagccg acgtcgggt 
      tgcctcgtc aatttaacc gaatggccc ccgttcgta 
      attacgctc gaagacgtc gaattagtc cacttcgaa 
      agagtccaa ttccatttg aaaaacttc gatatgatt 
      ttcgtcttc aaggactac caacgcaaa accgcaatg 
      gtgaacgcc atcccgatg aatatgctg gaccatgtc 
      aaagaatgg ctcaactct tgcgacatc cgctactcc 
      gaaggtgtc cagtcgtta aactgggtc aaaatcatg 
      aaaacaatc accgatgat cccgaggga tttttcgaa 
      agcggcgga tggacgttc ctggacccg gaatcggac 
      gaagaagcc caggcccaa gaggaggaa acggaggac 
      gaggacgaa gtttacgaa ccgaccgac atggaaagc 
      gtgtcggaa gaaagcgag gaagattcc gaatactct 
      gaaggcgac actgaggag agtgacgaa agcgcttcc 
      ggggaggac gatctagga tctgatgaa gaatccggg 
      aaggactgg tccgacctg gagcgggaa gcagccgag 
      gaggaccgg gaacgcaat ttcgacgat actttcgac 
      cggaaaaag tcgcatcac aagtcgagc aaacccagc 
      aaacactcg tcgagtaaa agcaaccac aaaggctcg 
      ccgagcaaa cattcaaac aaccatcat tcctcaagt 
      agcaaacat tcgagttcg aaacatagc agcagagat 
      agtgatagg cgtaaatct tcttcaagt tcgaaatcg 
      aaatcttca ccctcgaaa gacaaggac cgccatcac 
      agaagctcg cattcggat tcgaaaaag cgcagtcgg 
      gaggagagc agcgataaa cacaagtcc aagaagtcg 
      aagaagtaa ttcgaggaa gttgttttt accgttgta 
      tttgtataa gttattttt atgttagag atttgtgtt 
      tcaagttct gtgtttcgt tcaggctaa gttctttcc 
      actgaatgt acagattaa acttttgat ataaaccgt 
      ggtttaatt tgcacggaa ttcttagtt ttcgtgtat 
      tacatttta tgaagttat tgcaaaaca tgtaataaa 
      tttgaaaag accaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY