Status
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Gene information
General information
TC014287 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8446965..8455192
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014287
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05603

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014287 RA
      MVCKEDVLS WFKDLEGYK RIDVLYELL NMCVPFELR 
      FLGTCIEEL GKHTYQELR GPAINANDL DRLSKDTSF 
      SQSLLDENV RHRVLIYLS LLSSRNYSV ANWLYKNVL 
      RTDWLEDCI VKGNLKDEI VQSEFLLLF VMALHHPAF 
      TFEQKLHFN QILSLLLEL RESKKRISP KPSSYGYPP 
      GFGYPTAKM NIQEGPIVP VKTSMTIPC GEDTLHQQS 
      PGLPHVPPL QPHQEFVTW NIPGFACGA PDLGKVPPP 
      FPMPPTVSP LVSESTSPN HSRSTSPHR TIVTLRSTP 
      MPPPQTTRP IDTTIPTQL PIDPLSPNN FPFLHPAEN 
      VKMPDEDLN SIPEDKLLL EGSWVTVPM KEVKQPNGI 
      RLSACPTKN SHRHFVEHF QGLSLNDGE NSLHRSNSS 
      SSSSLNQTP PETPAATPH PPQKARVNG MPFIPPAPG 
      LPLCDTTSP PPPPTPTFN NCSVPYTTY QFTAIPTRS 
      MFPYNQSYR PPFPHFQSY QTNENSFQT YSYMPLMFS 
      SPFPPRNPN CYNCGAMGH SGQDCTGQN IEDITQKKT 
      YMIEFAPPP LPDADK
    CDS sequence
    • >TC014287 RA
      atggtgtgt aaagaggat gttttatca tggtttaag 
      gacttggag ggttacaag agaatagat gtactttac 
      gaactgttg aatatgtgc gtccctttc gagctccgt 
      ttcttgggc acctgcatc gaggagctg ggcaaacac 
      acctaccag gaactacgc ggaccagcc atcaacgcc 
      aacgacctg gacagactg tccaaggac acctcgttc 
      tcgcagagt ctcctcgac gagaacgtc aggcacaga 
      gtgctaatc tacctgtct ctgctctcc tcacggaac 
      tatagtgtt gccaattgg ctgtacaaa aacgttcta 
      aggacagac tggctcgag gattgtata gtgaaggga 
      aatcttaag gatgaaatt gttcagagt gaatttttg 
      ttgttgttt gtcatggct ttgcaccat cctgctttt 
      actttcgaa caaaagtta cattttaac caaatactc 
      agtctcctt ttagagctt agggaaagt aaaaaaagg 
      atttcgccc aagcctagc agttacgga tatccgccc 
      ggatttgga taccccacc gcaaaaatg aatattcaa 
      gagggtcca atcgtccct gtaaaaact tcaatgacc 
      atcccgtgc ggtgaggac acccttcac cagcaatcc 
      ccgggtctt ccacacgtt ccaccactt cagccgcat 
      caggagttc gtcacgtgg aacattccg ggctttgcg 
      tgtggagcg ccggatttg ggaaaggta ccgcccccg 
      ttcccgatg cccccaact gtatcaccc ctagtcagc 
      gaatcaacg tcaccgaac cacagtcgt agcacctca 
      ccgcatcga acaatagtc acgttacgg tcgacgccg 
      atgccgccc ccacagaca acccgcccg attgacacc 
      accataccg acgcagtta cccatcgac ccgctctct 
      ccaaataat ttccctttt cttcatccc gctgagaac 
      gttaaaatg cccgacgag gatctgaac tcgataccg 
      gaggacaag ctgttgttg gagggttct tgggtgacg 
      gtcccgatg aaagaagtg aagcagcca aacggtatt 
      cgcttgagc gcgtgtcct acaaaaaac agccatcgg 
      catttcgtg gagcatttt cagggactg agcctaaac 
      gacggcgaa aactcgctc caccgctcg aactcgtcc 
      agcagcagc agcttgaac caaacgccc cccgagact 
      ccggccgcg acgccgcac ccgccgcag aaggcccgc 
      gtgaacggc atgcccttc atcccgccg gcgccgggg 
      ctgccgctg tgcgacacg acgtcgccg ccgccgccg 
      ccgacgccc accttcaac aactgctcc gtgccttac 
      accacttat cagttcacc gccatcccc accaggtca 
      atgttccca tacaaccag tcgtaccga ccgccattc 
      ccgcacttt cagtcctat cagacgaac gaaaattcc 
      tttcagacc tactcctac atgcccctc atgttttcc 
      agtccgttc ccgcctagg aaccccaac tgctacaac 
      tgcggggcc atggggcac tccggacaa gactgcacg 
      gggcagaac atagaggat ataacgcag aaaaagacg 
      tacatgatt gagttcgcg ccgccgccg ctccctgac 
      gccgataaa tag
    mRNA sequence
    • >TC014287 RA
      tcgtttcaa ttcaccgaa agcctgaat aatgttcac 
      ctacttttt caaatttgg cgcgcctgg ttgttttga 
      agttggttc gttcggtcg ctaaagcac aaaatttgc 
      aacaataaa atttgacac atttttgga aatgtggca 
      acgcttctg tcccgaaaa tcgtgcaat ttttctctg 
      aaaataaaa caatcgggg taaaatcgg gcgcaaagt 
      gttaaaata atcccgaat tgcagtcat tttgtgaaa 
      aagtgggga ctgagcgga caaagctgc attctgcag 
      tgtgattcc cgaatgtca gaattgttt tgttgaatg 
      gttaggtgc tattttctg gctagttga gtcaagagg 
      accacattc cacctgcag tttcggcgt gtctttgtc 
      gcgtgttgc tgcgaccag cggaggcgc tgtcaatgg 
      gcatttggg cccgttcgc cccacggaa ggaccctct 
      ggtggtagc gtgcgctcg gcccgatgg aagtgctgc 
      ctctttagt gcgacaggc tctgatatg gtcttgaat 
      gcgttggat aactgtgct ttgtaacat caatggttt 
      ctggagatg gtgtgtaaa gaggatgtt ttatcatgg 
      tttaaggac ttggagggt tacaagaga atagatgta 
      ctttacgaa ctgttgaat atgtgcgtc cctttcgag 
      ctccgtttc ttgggcacc tgcatcgag gagctgggc 
      aaacacacc taccaggaa ctacgcgga ccagccatc 
      aacgccaac gacctggac agactgtcc aaggacacc 
      tcgttctcg cagagtctc ctcgacgag aacgtcagg 
      cacagagtg ctaatctac ctgtctctg ctctcctca 
      cggaactat agtgttgcc aattggctg tacaaaaac 
      gttctaagg acagactgg ctcgaggat tgtatagtg 
      aagggaaat cttaaggat gaaattgtt cagagtgaa 
      tttttgttg ttgtttgtc atggctttg caccatcct 
      gcttttact ttcgaacaa aagttacat tttaaccaa 
      atactcagt ctcctttta gagcttagg gaaagtaaa 
      aaaaggatt tcgcccaag cctagcagt tacggatat 
      ccgcccgga tttggatac cccaccgca aaaatgaat 
      attcaagag ggtccaatc gtccctgta aaaacttca 
      atgaccatc ccgtgcggt gaggacacc cttcaccag 
      caatccccg ggtcttcca cacgttcca ccacttcag 
      ccgcatcag gagttcgtc acgtggaac attccgggc 
      tttgcgtgt ggagcgccg gatttggga aaggtaccg 
      cccccgttc ccgatgccc ccaactgta tcaccccta 
      gtcagcgaa tcaacgtca ccgaaccac agtcgtagc 
      acctcaccg catcgaaca atagtcacg ttacggtcg 
      acgccgatg ccgccccca cagacaacc cgcccgatt 
      gacaccacc ataccgacg cagttaccc atcgacccg 
      ctctctcca aataatttc ccttttctt catcccgct 
      gagaacgtt aaaatgccc gacgaggat ctgaactcg 
      ataccggag gacaagctg ttgttggag ggttcttgg 
      gtgacggtc ccgatgaaa gaagtgaag cagccaaac 
      ggtattcgc ttgagcgcg tgtcctaca aaaaacagc 
      catcggcat ttcgtggag cattttcag ggactgagc 
      ctaaacgac ggcgaaaac tcgctccac cgctcgaac 
      tcgtccagc agcagcagc ttgaaccaa acgcccccc 
      gagactccg gccgcgacg ccgcacccg ccgcagaag 
      gcccgcgtg aacggcatg cccttcatc ccgccggcg 
      ccggggctg ccgctgtgc gacacgacg tcgccgccg 
      ccgccgccg acgcccacc ttcaacaac tgctccgtg 
      ccttacacc acttatcag ttcaccgcc atccccacc 
      aggtcaatg ttcccatac aaccagtcg taccgaccg 
      ccattcccg cactttcag tcctatcag acgaacgaa 
      aattccttt cagacctac tcctacatg cccctcatg 
      ttttccagt ccgttcccg cctaggaac cccaactgc 
      tacaactgc ggggccatg gggcactcc ggacaagac 
      tgcacgggg cagaacata gaggatata acgcagaaa 
      aagacgtac atgattgag ttcgcgccg ccgccgctc 
      cctgacgcc gataaatag tagtggttt cgtgttgtg 
      cataattgt ttctgtaat agtcacaag tgatttgga 
      cagttaccg tactgctat tgagacagt gattttgta 
      aaataggag gaaagatgt gaattattt gtaaaaaat 
      ctttaagct ttgatgttt tgttactaa aatatggcc 
      tggcgtcat agcactgat cgttcttag ctgtggtcc 
      gaactttac gataggtgg tatgtgcgt tttctaaat 
      actgttact tctttttat cacgttctt gtacagaat 
      ctcgaaagt tttacgtcc gcttttttg tgtcttgtc 
      aatttattt agtacaatt ttcagtagc gaatttttt 
      tctaagatg tacttactt catatggtg aagttccaa 
      tttttagac gttttaatt atatttaag caattgcca 
      caatattta taaggaagt gagattatt tattaattt 
      gttatattt gttttcaat actctcttt ttaactacc 
      aatcattgt cctatgcat aattctgta tacttctgt 
      catcatgat tttactgta catagtgac tagttatta 
      catagttaa tattaaata ttgatatat ttataatac 
      aatcagcat ttgactgaa agtttattt gaaagtaaa 
      gaataaaga ttttttaac aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY