| General information | |||
| TC014241 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 3583670..3589388 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014241 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05592 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
- >TC014241 RA
MYAKGKGST VPSDAQARE KLALYVYEY LLHVGAQKA AQTFLSEIR WEKNITLGE PPGFLHSWW CVFWDLYCA APERRDTCE HSSEAKAFH DYGFVNSGY GVNGIAHNT GPAPSPLGQ MPPADGMPG GPIPPGFFP NSTMRPSPP THPSSQPSP HSQPPPPHT QMMSAQPFM GPRYPAGPR PGVRMPQIA SDFNGPPGQ PMMPNNMDP TRQGPPGMN PMNRMNPPR GPSMGPLGP GTYGPSLRG PPPNSSLGP GGPMPPMSM TGPGGRPQW QPNTSTPMN YSSSSPGNY GGPPGSAGP PGPGTPIMP SPQDSSNSG GENMYTLMK PAVGGNMPG EFQMGGGPE GGPMGPMGP NSMGPVLNG DGMDGMKNS PANGGPGTP REDSGSGMG DYNLGGFGG PGENDQTES AAILKIKES MQEEAKRFE KDSDHPEYF IQ
- >TC014241 RB
MYAKGKGST VPSDAQARE KLALYVYEY LLHVGAQKA AQTFLSEIR WEKNITLGE PPGFLHSWW CVFWDLYCA APERRDTCE HSSEAKAFH DYGFVNSGY GVNGIAHNT GPAPSPLGQ MPPADGMPG GPIPPGFFP NSTMRPSPP THPSSQPSP HSQPPPPHT QMMSAQPFM GPRYPAGPR PGVRMPQIA SDFNGPPGQ PMMPNNMDP TRQGEGGEF VGWQGPPGM NPMNRMNPP RGPSMGPLG PGTYGPSLR GPPPNSSLG PGGPMPPMS MTGPGGRPQ WQPNTSTPM NYSSSSPGN YGGPPGSAG PPGPGTPIM PSPQDSSNS GGENMYTLM KPAVGGNMP GEFQMGGGP EGGPMGPMG PNSMGPVLN GDGMDGMKN SPANGGPGT PREDSGSGM GDYNLGGFG GPGENDQTE SAAILKIKE SMQEEAKRF EKDSDHPEY FIQ
- >TC014241 RA
atgtacgcg aaaggaaag ggatcaaca gtgccgtcc gatgcacaa gcacgagaa aagttggca ttatacgta tatgaatat ttacttcac gttggtgca caaaaggcc gcacaaaca tttctttca gagattcgg tgggagaag aatataacg ttaggagag ccccctgga tttttgcac tcttggtgg tgtgtattt tgggattta tattgcgcc gctccagaa aggagggat acctgcgag cattcatca gaagctaaa gcttttcat gattatggc tttgtaaat tctgggtat ggagttaat ggcattgca cataacact ggcccagca ccaagtccg ttgggacaa atgcctcca gctgacgga atgcctgga ggtcccata ccgccagga ttttttcca aattcaacg atgaggccg tcaccaccc acacaccct tcaagccaa ccctctccc cattcacag ccgcctcca cctcacaca caaatgatg tcggcgcaa cctttcatg ggtccccgg tatccggca ggtcctaga ccgggtgta aggatgccc caaatcgcg agcgatttt aacggaccc cccggacag ccaatgatg cccaataat atggatccg acgagacaa ggccccccg ggaatgaac ccaatgaat cgaatgaac cctcccaga ggaccttca atggggccg ctaggcccc gggacctac ggtcccagt ttacggggc ccaccacct aatagcagt ctaggacct ggtggcccc atgcccccc atgtccatg acgggcccc ggcggacgg ccgcagtgg cagcccaac acgtctaca ccaatgaac tattcttcc tcgtcgcct ggcaactac gggggcccg ccggggtcg gctgggccg ccgggcccc ggaaccccg ataatgccg agcccgcaa gattcgtcg aactcgggc ggcgagaac atgtacacg ttaatgaag ccggcggtt ggcggaaac atgcctgga gagttccag atgggtggt gggcccgaa ggagggccg atgggtccc atgggcccg aattcgatg ggtccagtg ctgaacggt gacggaatg gacggaatg aagaattcg ccggcgaat ggaggccca ggaacaccg cgagaggac agcggtagc ggaatgggc gactacaac ttaggggga tttggagga ccgggagaa aacgaccaa acggaatcg gctgctatt ttgaaaata aaagagagc atgcaagag gaggccaag aggtttgag aaagactcg gaccaccct gagtatttc atacaataa
- >TC014241 RB
atgtacgcg aaaggaaag ggatcaaca gtgccgtcc gatgcacaa gcacgagaa aagttggca ttatacgta tatgaatat ttacttcac gttggtgca caaaaggcc gcacaaaca tttctttca gagattcgg tgggagaag aatataacg ttaggagag ccccctgga tttttgcac tcttggtgg tgtgtattt tgggattta tattgcgcc gctccagaa aggagggat acctgcgag cattcatca gaagctaaa gcttttcat gattatggc tttgtaaat tctgggtat ggagttaat ggcattgca cataacact ggcccagca ccaagtccg ttgggacaa atgcctcca gctgacgga atgcctgga ggtcccata ccgccagga ttttttcca aattcaacg atgaggccg tcaccaccc acacaccct tcaagccaa ccctctccc cattcacag ccgcctcca cctcacaca caaatgatg tcggcgcaa cctttcatg ggtccccgg tatccggca ggtcctaga ccgggtgta aggatgccc caaatcgcg agcgatttt aacggaccc cccggacag ccaatgatg cccaataat atggatccg acgagacaa ggtgaaggc ggagaattt gtaggttgg caaggcccc ccgggaatg aacccaatg aatcgaatg aaccctccc agaggacct tcaatgggg ccgctaggc cccgggacc tacggtccc agtttacgg ggcccacca cctaatagc agtctagga cctggtggc cccatgccc cccatgtcc atgacgggc cccggcgga cggccgcag tggcagccc aacacgtct acaccaatg aactattct tcctcgtcg cctggcaac tacgggggc ccgccgggg tcggctggg ccgccgggc cccggaacc ccgataatg ccgagcccg caagattcg tcgaactcg ggcggcgag aacatgtac acgttaatg aagccggcg gttggcgga aacatgcct ggagagttc cagatgggt ggtgggccc gaaggaggg ccgatgggt cccatgggc ccgaattcg atgggtcca gtgctgaac ggtgacgga atggacgga atgaagaat tcgccggcg aatggaggc ccaggaaca ccgcgagag gacagcggt agcggaatg ggcgactac aacttaggg ggatttgga ggaccggga gaaaacgac caaacggaa tcggctgct attttgaaa ataaaagag agcatgcaa gaggaggcc aagaggttt gagaaagac tcggaccac cctgagtat ttcatacaa taa
- >TC014241 RA
caagtcatc aagaaggtc cacggttgc cagggtgat cgtggagtt aattgtatg ttgtggcta tttggccga aaaagcaat cgcaaaagc agcttttcc ctccaaaat tggccagaa ccggcgctc ggaaggtgc ccgatgtgc gtggggttg aatgtgcga attgcgcca attccttcc ggaaatcaa attattgga agagagtga ttatggtaa cgtgttatg gcaacatag gctacggga cagtggcgc gtgcgcaat cgggagtat tttagtgag aattttgag tgcggcgtc gaaagaatt taatgtgtg tattgttgc gttttcgat gtgcgtgtt tgttggcaa gttgtagat atttccaag tgatgttaa ttggggaaa agtaatctg gctgcggtg aagttgaac cggccggat taccatgaa ctggatctc tcgtcatat agaaccagc gtgttcgct caaatagtt aatttttaa gtaataata tatttaatt gtatgtagg ttaaattca gctctgtgt tcctttgta ttttaaaaa tgtacgcga aaggaaagg gatcaacag tgccgtccg atgcacaag cacgagaaa agttggcat tatacgtat atgaatatt tacttcacg ttggtgcac aaaaggccg cacaaacat ttctttcag agattcggt gggagaaga atataacgt taggagagc cccctggat ttttgcact cttggtggt gtgtatttt gggatttat attgcgccg ctccagaaa ggagggata cctgcgagc attcatcag aagctaaag cttttcatg attatggct ttgtaaatt ctgggtatg gagttaatg gcattgcac ataacactg gcccagcac caagtccgt tgggacaaa tgcctccag ctgacggaa tgcctggag gtcccatac cgccaggat tttttccaa attcaacga tgaggccgt caccaccca cacaccctt caagccaac cctctcccc attcacagc cgcctccac ctcacacac aaatgatgt cggcgcaac ctttcatgg gtccccggt atccggcag gtcctagac cgggtgtaa ggatgcccc aaatcgcga gcgatttta acggacccc ccggacagc caatgatgc ccaataata tggatccga cgagacaag gccccccgg gaatgaacc caatgaatc gaatgaacc ctcccagag gaccttcaa tggggccgc taggccccg ggacctacg gtcccagtt tacggggcc caccaccta atagcagtc taggacctg gtggcccca tgcccccca tgtccatga cgggccccg gcggacggc cgcagtggc agcccaaca cgtctacac caatgaact attcttcct cgtcgcctg gcaactacg ggggcccgc cggggtcgg ctgggccgc cgggccccg gaaccccga taatgccga gcccgcaag attcgtcga actcgggcg gcgagaaca tgtacacgt taatgaagc cggcggttg gcggaaaca tgcctggag agttccaga tgggtggtg ggcccgaag gagggccga tgggtccca tgggcccga attcgatgg gtccagtgc tgaacggtg acggaatgg acggaatga agaattcgc cggcgaatg gaggcccag gaacaccgc gagaggaca gcggtagcg gaatgggcg actacaact tagggggat ttggaggac cgggagaaa acgaccaaa cggaatcgg ctgctattt tgaaaataa aagagagca tgcaagagg aggccaaga ggtttgaga aagactcgg accaccctg agtatttca tacaataac tccctcgct ggcctcttt ggctggtgg acgccttta ttcctacct aacctgtac taacccggg cggtggtct tgtcagctc ttcctgccc tcatcgttg cgagctcgc caattttca actgtttga ctactacta gtcatgaat gttttctgt tttgcagat tttcctttg aaagctgaa tatacgata cgaagataa ggacattgt gtggctgta ctttggtgt aataaattt tcgagttcc tgataccta cttgtccac cagtctagg ctaggttgg atgtattca cggcgttct gtgtttatt ctatactaa gtactactt gcatgtgtc tacattcct ccacggagc aggttgcag atactgtag agcagcaat gattattgt ttgacataa tacacattg tagtttttt ttataaata tttatggaa gataagatt ttcagtcat tactaatcc tcattctat aatctgctc cgacctata atgtttctt caccttttt atgtgtaca tgcaataga caagtgcaa aaggagttt gctcctcta ttaaaatat tatgtttta tctgtaatt tctcaaaaa gcaacttaa ttcgtatgt atgtcatat acaataagt nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnna atagacaag tgcaaaagg agtttgctc ctctattaa aatattatg ttttatctg taatttctc aaaaagcaa cttaattcg tatgtatgt catatacaa taagttcga tttttaatt tgattcatt aatttatat attttgttc ttaatatat ttacacata tatttaaaa gattagatt attttatga atgaatgat gcgtgataa atattctga tgaactgga taaataaca ttgtgcaag tgagttctt tatctatat tgctttttt ttgtatggt ttattaaat attaaaagg ttca
- >TC014241 RB
caagtcatc aagaaggtc cacggttgc cagggtgat cgtggagtt aattgtatg ttgtggcta tttggccga aaaagcaat cgcaaaagc agcttttcc ctccaaaat tggccagaa ccggcgctc ggaaggtgc ccgatgtgc gtggggttg aatgtgcga attgcgcca attccttcc ggaaatcaa attattgga agagagtga ttatggtaa cgtgttatg gcaacatag gctacggga cagtggcgc gtgcgcaat cgggagtat tttagtgag aattttgag tgcggcgtc gaaagaatt taatgtgtg tattgttgc gttttcgat gtgcgtgtt tgttggcaa gttgtagat atttccaag tgatgttaa ttggggaaa agtaatctg gctgcggtg aagttgaac cggccggat taccatgaa ctggatctc tcgtcatat agaaccagc gtgttcgct caaatagtt aatttttaa gtaataata tatttaatt gtatgtagg ttaaattca gctctgtgt tcctttgta ttttaaaaa tgtacgcga aaggaaagg gatcaacag tgccgtccg atgcacaag cacgagaaa agttggcat tatacgtat atgaatatt tacttcacg ttggtgcac aaaaggccg cacaaacat ttctttcag agattcggt gggagaaga atataacgt taggagagc cccctggat ttttgcact cttggtggt gtgtatttt gggatttat attgcgccg ctccagaaa ggagggata cctgcgagc attcatcag aagctaaag cttttcatg attatggct ttgtaaatt ctgggtatg gagttaatg gcattgcac ataacactg gcccagcac caagtccgt tgggacaaa tgcctccag ctgacggaa tgcctggag gtcccatac cgccaggat tttttccaa attcaacga tgaggccgt caccaccca cacaccctt caagccaac cctctcccc attcacagc cgcctccac ctcacacac aaatgatgt cggcgcaac ctttcatgg gtccccggt atccggcag gtcctagac cgggtgtaa ggatgcccc aaatcgcga gcgatttta acggacccc ccggacagc caatgatgc ccaataata tggatccga cgagacaag gtgaaggcg gagaatttg taggttggc aaggccccc cgggaatga acccaatga atcgaatga accctccca gaggacctt caatggggc cgctaggcc ccgggacct acggtccca gtttacggg gcccaccac ctaatagca gtctaggac ctggtggcc ccatgcccc ccatgtcca tgacgggcc ccggcggac ggccgcagt ggcagccca acacgtcta caccaatga actattctt cctcgtcgc ctggcaact acgggggcc cgccggggt cggctgggc cgccgggcc ccggaaccc cgataatgc cgagcccgc aagattcgt cgaactcgg gcggcgaga acatgtaca cgttaatga agccggcgg ttggcggaa acatgcctg gagagttcc agatgggtg gtgggcccg aaggagggc cgatgggtc ccatgggcc cgaattcga tgggtccag tgctgaacg gtgacggaa tggacggaa tgaagaatt cgccggcga atggaggcc caggaacac cgcgagagg acagcggta gcggaatgg gcgactaca acttagggg gatttggag gaccgggag aaaacgacc aaacggaat cggctgcta ttttgaaaa taaaagaga gcatgcaag aggaggcca agaggtttg agaaagact cggaccacc ctgagtatt tcatacaat aactccctc gctggcctc tttggctgg tggacgcct ttattccta cctaacctg tactaaccc gggcggtgg tcttgtcag ctcttcctg ccctcatcg ttgcgagct cgccaattt tcaactgtt tgactacta ctagtcatg aatgttttc tgttttgca gattttcct ttgaaagct gaatatacg atacgaaga taaggacat tgtgtggct gtactttgg tgtaataaa ttttcgagt tcctgatac ctacttgtc caccagtct aggctaggt tggatgtat tcacggcgt tctgtgttt attctatac taagtacta cttgcatgt gtctacatt cctccacgg agcaggttg cagatactg tagagcagc aatgattat tgtttgaca taatacaca ttgtagttt tttttataa atatttatg gaagataag attttcagt cattactaa tcctcattc tataatctg ctccgacct ataatgttt cttcacctt tttatgtgt acatgcaat agacaagtg caaaaggag tttgctcct ctattaaaa tattatgtt ttatctgta atttctcaa aaagcaact taattcgta tgtatgtca tatacaata agtnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn nnaatagac aagtgcaaa aggagtttg ctcctctat taaaatatt atgttttat ctgtaattt ctcaaaaag caacttaat tcgtatgta tgtcatata caataagtt cgattttta atttgattc attaattta tatattttg ttcttaata tatttacac atatattta aaagattag attatttta tgaatgaat gatgcgtga taaatattc tgatgaact ggataaata acattgtgc aagtgagtt ctttatcta tattgcttt tttttgtat ggtttatta aatattaaa aggttca
