Status
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Gene information
General information
TC014241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3583670..3589388
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014241
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05592

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014241 RA
      MYAKGKGST VPSDAQARE KLALYVYEY LLHVGAQKA 
      AQTFLSEIR WEKNITLGE PPGFLHSWW CVFWDLYCA 
      APERRDTCE HSSEAKAFH DYGFVNSGY GVNGIAHNT 
      GPAPSPLGQ MPPADGMPG GPIPPGFFP NSTMRPSPP 
      THPSSQPSP HSQPPPPHT QMMSAQPFM GPRYPAGPR 
      PGVRMPQIA SDFNGPPGQ PMMPNNMDP TRQGPPGMN 
      PMNRMNPPR GPSMGPLGP GTYGPSLRG PPPNSSLGP 
      GGPMPPMSM TGPGGRPQW QPNTSTPMN YSSSSPGNY 
      GGPPGSAGP PGPGTPIMP SPQDSSNSG GENMYTLMK 
      PAVGGNMPG EFQMGGGPE GGPMGPMGP NSMGPVLNG 
      DGMDGMKNS PANGGPGTP REDSGSGMG DYNLGGFGG 
      PGENDQTES AAILKIKES MQEEAKRFE KDSDHPEYF 
      IQ
    • >TC014241 RB
      MYAKGKGST VPSDAQARE KLALYVYEY LLHVGAQKA 
      AQTFLSEIR WEKNITLGE PPGFLHSWW CVFWDLYCA 
      APERRDTCE HSSEAKAFH DYGFVNSGY GVNGIAHNT 
      GPAPSPLGQ MPPADGMPG GPIPPGFFP NSTMRPSPP 
      THPSSQPSP HSQPPPPHT QMMSAQPFM GPRYPAGPR 
      PGVRMPQIA SDFNGPPGQ PMMPNNMDP TRQGEGGEF 
      VGWQGPPGM NPMNRMNPP RGPSMGPLG PGTYGPSLR 
      GPPPNSSLG PGGPMPPMS MTGPGGRPQ WQPNTSTPM 
      NYSSSSPGN YGGPPGSAG PPGPGTPIM PSPQDSSNS 
      GGENMYTLM KPAVGGNMP GEFQMGGGP EGGPMGPMG 
      PNSMGPVLN GDGMDGMKN SPANGGPGT PREDSGSGM 
      GDYNLGGFG GPGENDQTE SAAILKIKE SMQEEAKRF 
      EKDSDHPEY FIQ
    CDS sequence
    • >TC014241 RA
      atgtacgcg aaaggaaag ggatcaaca gtgccgtcc 
      gatgcacaa gcacgagaa aagttggca ttatacgta 
      tatgaatat ttacttcac gttggtgca caaaaggcc 
      gcacaaaca tttctttca gagattcgg tgggagaag 
      aatataacg ttaggagag ccccctgga tttttgcac 
      tcttggtgg tgtgtattt tgggattta tattgcgcc 
      gctccagaa aggagggat acctgcgag cattcatca 
      gaagctaaa gcttttcat gattatggc tttgtaaat 
      tctgggtat ggagttaat ggcattgca cataacact 
      ggcccagca ccaagtccg ttgggacaa atgcctcca 
      gctgacgga atgcctgga ggtcccata ccgccagga 
      ttttttcca aattcaacg atgaggccg tcaccaccc 
      acacaccct tcaagccaa ccctctccc cattcacag 
      ccgcctcca cctcacaca caaatgatg tcggcgcaa 
      cctttcatg ggtccccgg tatccggca ggtcctaga 
      ccgggtgta aggatgccc caaatcgcg agcgatttt 
      aacggaccc cccggacag ccaatgatg cccaataat 
      atggatccg acgagacaa ggccccccg ggaatgaac 
      ccaatgaat cgaatgaac cctcccaga ggaccttca 
      atggggccg ctaggcccc gggacctac ggtcccagt 
      ttacggggc ccaccacct aatagcagt ctaggacct 
      ggtggcccc atgcccccc atgtccatg acgggcccc 
      ggcggacgg ccgcagtgg cagcccaac acgtctaca 
      ccaatgaac tattcttcc tcgtcgcct ggcaactac 
      gggggcccg ccggggtcg gctgggccg ccgggcccc 
      ggaaccccg ataatgccg agcccgcaa gattcgtcg 
      aactcgggc ggcgagaac atgtacacg ttaatgaag 
      ccggcggtt ggcggaaac atgcctgga gagttccag 
      atgggtggt gggcccgaa ggagggccg atgggtccc 
      atgggcccg aattcgatg ggtccagtg ctgaacggt 
      gacggaatg gacggaatg aagaattcg ccggcgaat 
      ggaggccca ggaacaccg cgagaggac agcggtagc 
      ggaatgggc gactacaac ttaggggga tttggagga 
      ccgggagaa aacgaccaa acggaatcg gctgctatt 
      ttgaaaata aaagagagc atgcaagag gaggccaag 
      aggtttgag aaagactcg gaccaccct gagtatttc 
      atacaataa
    • >TC014241 RB
      atgtacgcg aaaggaaag ggatcaaca gtgccgtcc 
      gatgcacaa gcacgagaa aagttggca ttatacgta 
      tatgaatat ttacttcac gttggtgca caaaaggcc 
      gcacaaaca tttctttca gagattcgg tgggagaag 
      aatataacg ttaggagag ccccctgga tttttgcac 
      tcttggtgg tgtgtattt tgggattta tattgcgcc 
      gctccagaa aggagggat acctgcgag cattcatca 
      gaagctaaa gcttttcat gattatggc tttgtaaat 
      tctgggtat ggagttaat ggcattgca cataacact 
      ggcccagca ccaagtccg ttgggacaa atgcctcca 
      gctgacgga atgcctgga ggtcccata ccgccagga 
      ttttttcca aattcaacg atgaggccg tcaccaccc 
      acacaccct tcaagccaa ccctctccc cattcacag 
      ccgcctcca cctcacaca caaatgatg tcggcgcaa 
      cctttcatg ggtccccgg tatccggca ggtcctaga 
      ccgggtgta aggatgccc caaatcgcg agcgatttt 
      aacggaccc cccggacag ccaatgatg cccaataat 
      atggatccg acgagacaa ggtgaaggc ggagaattt 
      gtaggttgg caaggcccc ccgggaatg aacccaatg 
      aatcgaatg aaccctccc agaggacct tcaatgggg 
      ccgctaggc cccgggacc tacggtccc agtttacgg 
      ggcccacca cctaatagc agtctagga cctggtggc 
      cccatgccc cccatgtcc atgacgggc cccggcgga 
      cggccgcag tggcagccc aacacgtct acaccaatg 
      aactattct tcctcgtcg cctggcaac tacgggggc 
      ccgccgggg tcggctggg ccgccgggc cccggaacc 
      ccgataatg ccgagcccg caagattcg tcgaactcg 
      ggcggcgag aacatgtac acgttaatg aagccggcg 
      gttggcgga aacatgcct ggagagttc cagatgggt 
      ggtgggccc gaaggaggg ccgatgggt cccatgggc 
      ccgaattcg atgggtcca gtgctgaac ggtgacgga 
      atggacgga atgaagaat tcgccggcg aatggaggc 
      ccaggaaca ccgcgagag gacagcggt agcggaatg 
      ggcgactac aacttaggg ggatttgga ggaccggga 
      gaaaacgac caaacggaa tcggctgct attttgaaa 
      ataaaagag agcatgcaa gaggaggcc aagaggttt 
      gagaaagac tcggaccac cctgagtat ttcatacaa 
      taa
    mRNA sequence
    • >TC014241 RA
      caagtcatc aagaaggtc cacggttgc cagggtgat 
      cgtggagtt aattgtatg ttgtggcta tttggccga 
      aaaagcaat cgcaaaagc agcttttcc ctccaaaat 
      tggccagaa ccggcgctc ggaaggtgc ccgatgtgc 
      gtggggttg aatgtgcga attgcgcca attccttcc 
      ggaaatcaa attattgga agagagtga ttatggtaa 
      cgtgttatg gcaacatag gctacggga cagtggcgc 
      gtgcgcaat cgggagtat tttagtgag aattttgag 
      tgcggcgtc gaaagaatt taatgtgtg tattgttgc 
      gttttcgat gtgcgtgtt tgttggcaa gttgtagat 
      atttccaag tgatgttaa ttggggaaa agtaatctg 
      gctgcggtg aagttgaac cggccggat taccatgaa 
      ctggatctc tcgtcatat agaaccagc gtgttcgct 
      caaatagtt aatttttaa gtaataata tatttaatt 
      gtatgtagg ttaaattca gctctgtgt tcctttgta 
      ttttaaaaa tgtacgcga aaggaaagg gatcaacag 
      tgccgtccg atgcacaag cacgagaaa agttggcat 
      tatacgtat atgaatatt tacttcacg ttggtgcac 
      aaaaggccg cacaaacat ttctttcag agattcggt 
      gggagaaga atataacgt taggagagc cccctggat 
      ttttgcact cttggtggt gtgtatttt gggatttat 
      attgcgccg ctccagaaa ggagggata cctgcgagc 
      attcatcag aagctaaag cttttcatg attatggct 
      ttgtaaatt ctgggtatg gagttaatg gcattgcac 
      ataacactg gcccagcac caagtccgt tgggacaaa 
      tgcctccag ctgacggaa tgcctggag gtcccatac 
      cgccaggat tttttccaa attcaacga tgaggccgt 
      caccaccca cacaccctt caagccaac cctctcccc 
      attcacagc cgcctccac ctcacacac aaatgatgt 
      cggcgcaac ctttcatgg gtccccggt atccggcag 
      gtcctagac cgggtgtaa ggatgcccc aaatcgcga 
      gcgatttta acggacccc ccggacagc caatgatgc 
      ccaataata tggatccga cgagacaag gccccccgg 
      gaatgaacc caatgaatc gaatgaacc ctcccagag 
      gaccttcaa tggggccgc taggccccg ggacctacg 
      gtcccagtt tacggggcc caccaccta atagcagtc 
      taggacctg gtggcccca tgcccccca tgtccatga 
      cgggccccg gcggacggc cgcagtggc agcccaaca 
      cgtctacac caatgaact attcttcct cgtcgcctg 
      gcaactacg ggggcccgc cggggtcgg ctgggccgc 
      cgggccccg gaaccccga taatgccga gcccgcaag 
      attcgtcga actcgggcg gcgagaaca tgtacacgt 
      taatgaagc cggcggttg gcggaaaca tgcctggag 
      agttccaga tgggtggtg ggcccgaag gagggccga 
      tgggtccca tgggcccga attcgatgg gtccagtgc 
      tgaacggtg acggaatgg acggaatga agaattcgc 
      cggcgaatg gaggcccag gaacaccgc gagaggaca 
      gcggtagcg gaatgggcg actacaact tagggggat 
      ttggaggac cgggagaaa acgaccaaa cggaatcgg 
      ctgctattt tgaaaataa aagagagca tgcaagagg 
      aggccaaga ggtttgaga aagactcgg accaccctg 
      agtatttca tacaataac tccctcgct ggcctcttt 
      ggctggtgg acgccttta ttcctacct aacctgtac 
      taacccggg cggtggtct tgtcagctc ttcctgccc 
      tcatcgttg cgagctcgc caattttca actgtttga 
      ctactacta gtcatgaat gttttctgt tttgcagat 
      tttcctttg aaagctgaa tatacgata cgaagataa 
      ggacattgt gtggctgta ctttggtgt aataaattt 
      tcgagttcc tgataccta cttgtccac cagtctagg 
      ctaggttgg atgtattca cggcgttct gtgtttatt 
      ctatactaa gtactactt gcatgtgtc tacattcct 
      ccacggagc aggttgcag atactgtag agcagcaat 
      gattattgt ttgacataa tacacattg tagtttttt 
      ttataaata tttatggaa gataagatt ttcagtcat 
      tactaatcc tcattctat aatctgctc cgacctata 
      atgtttctt caccttttt atgtgtaca tgcaataga 
      caagtgcaa aaggagttt gctcctcta ttaaaatat 
      tatgtttta tctgtaatt tctcaaaaa gcaacttaa 
      ttcgtatgt atgtcatat acaataagt nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnna atagacaag tgcaaaagg agtttgctc 
      ctctattaa aatattatg ttttatctg taatttctc 
      aaaaagcaa cttaattcg tatgtatgt catatacaa 
      taagttcga tttttaatt tgattcatt aatttatat 
      attttgttc ttaatatat ttacacata tatttaaaa 
      gattagatt attttatga atgaatgat gcgtgataa 
      atattctga tgaactgga taaataaca ttgtgcaag 
      tgagttctt tatctatat tgctttttt ttgtatggt 
      ttattaaat attaaaagg ttca
    • >TC014241 RB
      caagtcatc aagaaggtc cacggttgc cagggtgat 
      cgtggagtt aattgtatg ttgtggcta tttggccga 
      aaaagcaat cgcaaaagc agcttttcc ctccaaaat 
      tggccagaa ccggcgctc ggaaggtgc ccgatgtgc 
      gtggggttg aatgtgcga attgcgcca attccttcc 
      ggaaatcaa attattgga agagagtga ttatggtaa 
      cgtgttatg gcaacatag gctacggga cagtggcgc 
      gtgcgcaat cgggagtat tttagtgag aattttgag 
      tgcggcgtc gaaagaatt taatgtgtg tattgttgc 
      gttttcgat gtgcgtgtt tgttggcaa gttgtagat 
      atttccaag tgatgttaa ttggggaaa agtaatctg 
      gctgcggtg aagttgaac cggccggat taccatgaa 
      ctggatctc tcgtcatat agaaccagc gtgttcgct 
      caaatagtt aatttttaa gtaataata tatttaatt 
      gtatgtagg ttaaattca gctctgtgt tcctttgta 
      ttttaaaaa tgtacgcga aaggaaagg gatcaacag 
      tgccgtccg atgcacaag cacgagaaa agttggcat 
      tatacgtat atgaatatt tacttcacg ttggtgcac 
      aaaaggccg cacaaacat ttctttcag agattcggt 
      gggagaaga atataacgt taggagagc cccctggat 
      ttttgcact cttggtggt gtgtatttt gggatttat 
      attgcgccg ctccagaaa ggagggata cctgcgagc 
      attcatcag aagctaaag cttttcatg attatggct 
      ttgtaaatt ctgggtatg gagttaatg gcattgcac 
      ataacactg gcccagcac caagtccgt tgggacaaa 
      tgcctccag ctgacggaa tgcctggag gtcccatac 
      cgccaggat tttttccaa attcaacga tgaggccgt 
      caccaccca cacaccctt caagccaac cctctcccc 
      attcacagc cgcctccac ctcacacac aaatgatgt 
      cggcgcaac ctttcatgg gtccccggt atccggcag 
      gtcctagac cgggtgtaa ggatgcccc aaatcgcga 
      gcgatttta acggacccc ccggacagc caatgatgc 
      ccaataata tggatccga cgagacaag gtgaaggcg 
      gagaatttg taggttggc aaggccccc cgggaatga 
      acccaatga atcgaatga accctccca gaggacctt 
      caatggggc cgctaggcc ccgggacct acggtccca 
      gtttacggg gcccaccac ctaatagca gtctaggac 
      ctggtggcc ccatgcccc ccatgtcca tgacgggcc 
      ccggcggac ggccgcagt ggcagccca acacgtcta 
      caccaatga actattctt cctcgtcgc ctggcaact 
      acgggggcc cgccggggt cggctgggc cgccgggcc 
      ccggaaccc cgataatgc cgagcccgc aagattcgt 
      cgaactcgg gcggcgaga acatgtaca cgttaatga 
      agccggcgg ttggcggaa acatgcctg gagagttcc 
      agatgggtg gtgggcccg aaggagggc cgatgggtc 
      ccatgggcc cgaattcga tgggtccag tgctgaacg 
      gtgacggaa tggacggaa tgaagaatt cgccggcga 
      atggaggcc caggaacac cgcgagagg acagcggta 
      gcggaatgg gcgactaca acttagggg gatttggag 
      gaccgggag aaaacgacc aaacggaat cggctgcta 
      ttttgaaaa taaaagaga gcatgcaag aggaggcca 
      agaggtttg agaaagact cggaccacc ctgagtatt 
      tcatacaat aactccctc gctggcctc tttggctgg 
      tggacgcct ttattccta cctaacctg tactaaccc 
      gggcggtgg tcttgtcag ctcttcctg ccctcatcg 
      ttgcgagct cgccaattt tcaactgtt tgactacta 
      ctagtcatg aatgttttc tgttttgca gattttcct 
      ttgaaagct gaatatacg atacgaaga taaggacat 
      tgtgtggct gtactttgg tgtaataaa ttttcgagt 
      tcctgatac ctacttgtc caccagtct aggctaggt 
      tggatgtat tcacggcgt tctgtgttt attctatac 
      taagtacta cttgcatgt gtctacatt cctccacgg 
      agcaggttg cagatactg tagagcagc aatgattat 
      tgtttgaca taatacaca ttgtagttt tttttataa 
      atatttatg gaagataag attttcagt cattactaa 
      tcctcattc tataatctg ctccgacct ataatgttt 
      cttcacctt tttatgtgt acatgcaat agacaagtg 
      caaaaggag tttgctcct ctattaaaa tattatgtt 
      ttatctgta atttctcaa aaagcaact taattcgta 
      tgtatgtca tatacaata agtnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnaatagac aagtgcaaa aggagtttg ctcctctat 
      taaaatatt atgttttat ctgtaattt ctcaaaaag 
      caacttaat tcgtatgta tgtcatata caataagtt 
      cgattttta atttgattc attaattta tatattttg 
      ttcttaata tatttacac atatattta aaagattag 
      attatttta tgaatgaat gatgcgtga taaatattc 
      tgatgaact ggataaata acattgtgc aagtgagtt 
      ctttatcta tattgcttt tttttgtat ggtttatta 
      aatattaaa aggttca
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