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Gene information
General information
TC014237 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3545064..3547188
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014237
    iBeetle screen iB_05591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014237 RA
      MDLKRKLPI FIWATVIFC IKAQSISDS SNAFAIDLL 
      AATSKEPRS PRNLALSPY TVWTLLSII DEGAKDKTA 
      KQMESVLRI PSFPDKAMF RNSFKNLSA LLSEKDDGV 
      NLDIYNAIF TTKAQELKT DFRDIAHKD YGVKIEPTD 
      FSDTRTASD RINKYVASA TKNRIRNFI RPDDVFDAE 
      IFIVSAMYF KGTWMKPFN RSETRRDTF YDEKQNKLG 
      EVDMMSQMG FFPYSRLEW IRGYAIELP YGKGDKMSM 
      IVILPYKDQ TLANMLNLM AEHPFSRII DRLNKAKED 
      FEGEDVKVY LPRFTVKSD LTLNTVLEK MGMPDVFDA 
      SQANLLGMF PHYLYISRL LQRAEIQVN EEGTVATAA 
      SGASAVNKA PPPKFVANK PFLYFIVNK PAKSIVFAG 
      KVSVPP
    CDS sequence
    • >TC014237 RA
      atggatttg aagagaaag ttaccgatt ttcatctgg 
      gcgacggtg attttttgc attaaggcc caaagtatc 
      agcgactcg tcgaatgcg tttgcaatc gatttacta 
      gcggctacc agtaaagaa ccgagaagt ccacggaac 
      ttagcactg tctccgtac acagtgtgg acacttctc 
      tcgatcatc gatgaagga gcaaaggac aaaacggcc 
      aaacagatg gaaagcgtc ctcaggata ccaagcttt 
      cccgacaag gctatgttt aggaacagc ttcaaaaat 
      ttgtcggcc cttttatcg gaaaaagac gacggggtg 
      aacctagac atctacaac gctattttt accacaaaa 
      gcccaggaa ctgaaaacc gacttccgc gacattgcc 
      cacaaagat tacggagtc aaaatagaa cccaccgat 
      ttttcagac accagaacc gcgagcgat agaatcaac 
      aagtacgtg gctagcgcg acaaaaaac cgaatacga 
      aattttata cgcccagac gatgttttt gacgctgaa 
      attttcatc gtcagtgcc atgtatttc aaaggcacg 
      tggatgaaa cccttcaat aggtctgaa acgcgccgt 
      gacactttc tacgacgag aaacagaac aaacttggg 
      gaggtcgac atgatgtcg cagatgggc ttcttcccc 
      tattcgaga ctcgagtgg atcaggggc tacgccata 
      gagttaccc tacggaaag ggcgacaaa atgtcaatg 
      attgtgatt ttgccgtac aaagatcag acgcttgcg 
      aacatgctc aatttaatg gccgagcat ccgttcagc 
      aggatcatc gaccggctc aacaaagcc aaggaggat 
      tttgaaggc gaggatgtc aaagtgtat ttgccacgt 
      tttaccgtc aagtcggac ttgacactg aacacagta 
      cttgaaaag atgggaatg ccggacgtg ttcgacgcc 
      agtcaggcg aacctcctc ggcatgttc ccccactat 
      ttgtacatt tctcgactg ctccaaagg gcggaaata 
      caagtaaac gaagaagga actgtggca accgcagct 
      tctggtgcg tcagctgtg aataaagcc cctcctccg 
      aaatttgta gctaacaaa cccttcttg tacttcatt 
      gtgaacaag ccggcgaaa agtatcgtg tttgcggga 
      aaggtgagc gtcccccca tga
    mRNA sequence
    • >TC014237 RA
      atacaataa cgagtgaca tcaattttt ttatttgaa 
      acaagtttc cggatgttg atggtttca agagaggga 
      tagccaaat gggaaaccg atagatata aatccgtct 
      tcgttgaaa aagcaagga actgttcga gattcaagt 
      cgctgggaa aaacccaat gaatatctt taacaagct 
      ggtaagacg ttctggctg tgtacgtgc gtaagtatt 
      aggataaac actaaaatt tgtgctata aaaggaaaa 
      aatattaac gggatagtt aacagataa aggtcaacc 
      gcgctaact cgggggata tcaaaattt cttcacaaa 
      acgagttga acgcaaatt accgccgac gagtttaat 
      tgcatccag tgttttcta atcgcttgt gaggaaggc 
      actgcagtt gctgatgta ccaacaaca acatggatt 
      tgaagagaa agttaccga ttttcatct gggcgacgg 
      tgatttttt gcattaagg cccaaagta tcagcgact 
      cgtcgaatg cgtttgcaa tcgatttac tagcggcta 
      ccagtaaag aaccgagaa gtccacgga acttagcac 
      tgtctccgt acacagtgt ggacacttc tctcgatca 
      tcgatgaag gagcaaagg acaaaacgg ccaaacaga 
      tggaaagcg tcctcagga taccaagct ttcccgaca 
      aggctatgt ttaggaaca gcttcaaaa atttgtcgg 
      cccttttat cggaaaaag acgacgggg tgaacctag 
      acatctaca acgctattt ttaccacaa aagcccagg 
      aactgaaaa ccgacttcc gcgacattg cccacaaag 
      attacggag tcaaaatag aacccaccg atttttcag 
      acaccagaa ccgcgagcg atagaatca acaagtacg 
      tggctagcg cgacaaaaa accgaatac gaaatttta 
      tacgcccag acgatgttt ttgacgctg aaattttca 
      tcgtcagtg ccatgtatt tcaaaggca cgtggatga 
      aacccttca ataggtctg aaacgcgcc gtgacactt 
      tctacgacg agaaacaga acaaacttg gggaggtcg 
      acatgatgt cgcagatgg gcttcttcc cctattcga 
      gactcgagt ggatcaggg gctacgcca tagagttac 
      cctacggaa agggcgaca aaatgtcaa tgattgtga 
      ttttgccgt acaaagatc agacgcttg cgaacatgc 
      tcaatttaa tggccgagc atccgttca gcaggatca 
      tcgaccggc tcaacaaag ccaaggagg attttgaag 
      gcgaggatg tcaaagtgt atttgccac gttttaccg 
      tcaagtcgg acttgacac tgaacacag tacttgaaa 
      agatgggaa tgccggacg tgttcgacg ccagtcagg 
      cgaacctcc tcggcatgt tcccccact atttgtaca 
      tttctcgac tgctccaaa gggcggaaa tacaagtaa 
      acgaagaag gaactgtgg caaccgcag cttctggtg 
      cgtcagctg tgaataaag cccctcctc cgaaatttg 
      tagctaaca aacccttct tgtacttca ttgtgaaca 
      agccggcga aaagtatcg tgtttgcgg gaaaggtga 
      gcgtccccc catgaaata aaaaacgaa atgttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY