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Gene information
General information
TC014214 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3305749..3308038
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014214
    iBeetle screen iB_05589

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014214 RA
      MSGFDDNPF GEPTIDNPF ADPSVQQVA RNTNAQINV 
      NDYNPFDNQ PQNTRPMGY NQPNTTPAI MQPTQEPPA 
      YTKSGQQIH EPKPALNTE ELQRRQEEL ERKEEELAR 
      REEEMRQSS YNVRRNNWP PLPEQCCFQ PCFYQDIQV 
      DIPLEFQKI VRYLYYLWI FHGIIMLVN VLGGIVLLD 
      FMIIGLGIL YMVLFTPFS YLCWFRPAY KAFRSDSSF 
      NFMVFFFIF FFQFVVTLI QAIGIPGSG TIGIFTAIK 
      TFNNEAWSI IKGILALII AAGFGCAAA ADLFLITKI 
      HRMYRSTGA SLAKAQQEF TTEFLRNQH VRNAASNVA 
      AAAVQSQFS QNTNPRY
    CDS sequence
    • >TC014214 RA
      atgtccggt tttgacgat aatcccttc ggtgagcca 
      acaattgac aaccctttt gcggatcct tcggttcaa 
      caggttgcc aggaacacc aatgcccaa atcaatgtc 
      aatgattac aacccgttt gataatcaa ccccaaaac 
      acaaggcct atgggatac aaccaaccc aacacgacg 
      cctgcgatc atgcagccg acacaagaa ccacctgcc 
      tacaccaaa agcggccaa cagatacac gagcccaag 
      ccggctctc aacacggaa gagctgcaa agacgtcag 
      gaagagctg gagaggaag gaagaagag ttagctcga 
      cgggaggaa gaaatgcgg caatcgtcg tataatgta 
      agacgtaac aattggccg cctcttccc gaacagtgt 
      tgctttcaa ccgtgcttc taccaagac atccaagtg 
      gatatacct ttagaattc caaaaaata gtgcgttac 
      ttgtactat ctttggata tttcacgga ataataatg 
      ttggtgaac gtcctaggc ggaatagta ttgctagac 
      tttatgatt atcggactt ggaattttg tatatggtg 
      ttgtttacc ccgttttct tacttgtgc tggttccga 
      ccggcatat aaagcattc cgttccgac tcgtcgttc 
      aatttcatg gtgttcttt ttcatattc ttctttcag 
      tttgttgtt acacttata caagcaatc ggaattccc 
      ggttccggg actattggg atctttact gccattaaa 
      acgttcaat aacgaagcc tggagcata atcaaagga 
      attctggcg ctgattatt gcagcaggg tttgggtgt 
      gcagcagct gctgacttg tttttgatc acgaagatt 
      catcgcatg tacaggagt accggagct agtttagcc 
      aaagctcaa caagaattc acgactgag tttctgcgt 
      aatcagcat gtgcgaaac gctgcaagt aacgtcgct 
      gcagcggct gtgcaatca cagtttagc caaaacaca 
      aatcctaga tactaa
    mRNA sequence
    • >TC014214 RA
      gtttgttac attgcaagg tgtgtttca aacaaggat 
      agccgtaaa aattgtcgt gcgcatgat caccacaag 
      tgtcaacat ggctaaaaa ttaaaatat ggtgtcctg 
      tcaaaaacg ataatggat ttgtagata agattagaa 
      atcgctttc aagatccgt tgtttgcag aacgaagac 
      aaatatttt tgaaaaaat gtccggttt tgacgataa 
      tcccttcgg tgagccaac aattgacaa cccttttgc 
      ggatccttc ggttcaaca ggttgccag gaacaccaa 
      tgcccaaat caatgtcaa tgattacaa cccgtttga 
      taatcaacc ccaaaacac aaggcctat gggatacaa 
      ccaacccaa cacgacgcc tgcgatcat gcagccgac 
      acaagaacc acctgccta caccaaaag cggccaaca 
      gatacacga gcccaagcc ggctctcaa cacggaaga 
      gctgcaaag acgtcagga agagctgga gaggaagga 
      agaagagtt agctcgacg ggaggaaga aatgcggca 
      atcgtcgta taatgtaag acgtaacaa ttggccgcc 
      tcttcccga acagtgttg ctttcaacc gtgcttcta 
      ccaagacat ccaagtgga tataccttt agaattcca 
      aaaaatagt gcgttactt gtactatct ttggatatt 
      tcacggaat aataatgtt ggtgaacgt cctaggcgg 
      aatagtatt gctagactt tatgattat cggacttgg 
      aattttgta tatggtgtt gtttacccc gttttctta 
      cttgtgctg gttccgacc ggcatataa agcattccg 
      ttccgactc gtcgttcaa tttcatggt gttcttttt 
      catattctt ctttcagtt tgttgttac acttataca 
      agcaatcgg aattcccgg ttccgggac tattgggat 
      ctttactgc cattaaaac gttcaataa cgaagcctg 
      gagcataat caaaggaat tctggcgct gattattgc 
      agcagggtt tgggtgtgc agcagctgc tgacttgtt 
      tttgatcac gaagattca tcgcatgta caggagtac 
      cggagctag tttagccaa agctcaaca agaattcac 
      gactgagtt tctgcgtaa tcagcatgt gcgaaacgc 
      tgcaagtaa cgtcgctgc agcggctgt gcaatcaca 
      gtttagcca aaacacaaa tcctagata ctaaagatt 
      agtccaact gttgtactc ttcgatctt tctcagtat 
      ttagtcttt tttaattgc gttgattgt tattcaaat 
      taaaacact tgtgaacgt gaattaaac tgaaaatca 
      catgtctat ggttttgtt atataaata tgtatattt 
      ctctgatga aaatctatg tctggagaa gtcggaatt 
      gttaggttg tattgtaaa tatatattt ttgcattag 
      tttcctaat ggtttaaga atcatttga taaacaaaa 
      cacagcttg tacttaatc ataggcttt gttacaatt 
      aatcttttt tcatcactg aataatatc ctagagtag 
      ttgtctgtg ttatttatg taatgatta tccggtact 
      ttgtatatt gattttatt aatctgttc attcgttcc 
      agttttctt attgttttg taacatgta agatacact 
      aatatatta taaaaaacc gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY