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Gene information
General information
TC014213 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3299859..3302451
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014213
    iBeetle screen iB_05588

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014213 RA
      MGVPWLAQV RLLHMAILV LATASCLLY VAYITSPQL 
      TTTASPLRT LVSSEIRAF QGNTTQAEV AKNMTVAPP 
      SSNSGGSSE APQLPAVRT LTNATNSSQ PDLTRGVAA 
      EIIYEAGHV DVSSQICPE LGRDLKLLI AITSAPSHE 
      SARMAIRET WGHFASRKD VAIAFMLGS ISNETVNAN 
      IEKEQYLYG DIIRGKFRD TYDNLTLKT ISMLEWVDN 
      YCPKAAFVL KTDDDMFIN VSRLLAFIA KHSPEQRTI 
      YGRLAKKWK PIRNKKSKY YISPNQYKP AVFPDFTTG 
      PAYLLPARL SKELYVAAL NHTYFKLED VFVTGIVAN 
      SLKIKRVHA PEFLNKRVS LTPCSVQKG ISIHMVKGV 
      EQYDLWKKL HDVAAKCKK
    CDS sequence
    • >TC014213 RA
      atgggggtg ccatggctc gcccaagtg cggttgctg 
      cacatggcg attttagtt ctagcgacg gcgtcatgt 
      ctactgtac gtggcatat ataacatcc ccccaattg 
      acgacaaca gcttcaccc ctacgtacg ttagtatca 
      tccgaaatt cgcgcattc caaggtaac acgacccag 
      gctgaagtg gcgaagaac atgaccgtg gcgccgcct 
      tcctcgaac agcggcggc tcgtctgaa gcgccccaa 
      ttgccagca gttcgcacg ctgacaaat gcgacaaac 
      agcagtcag cccgaccta actcgggga gtcgccgct 
      gagataata tacgaggcc ggtcatgtg gacgtcagt 
      tcgcagatc tgccccgag ttaggcaga gatttaaag 
      ttgttgatt gcgataacg tcggctccc agccacgaa 
      agcgcccga atggcgatt cgggagact tggggccac 
      ttcgcgagc agaaaagac gtagctata gcatttatg 
      ttagggtcg atttcgaac gagacggtc aacgccaac 
      atagagaag gagcaatac ttgtacggt gatataatt 
      cgggggaag ttccgagac acatatgac aatcttact 
      cttaaaacg atttcgatg ttagaatgg gtggataac 
      tactgtcca aaagcagct tttgtactt aaaactgac 
      gacgacatg tttattaac gtctcccgg ctgctggct 
      ttcattgca aagcacagt ccggagcaa aggacgatc 
      tatggccgc ttggccaag aagtggaaa ccgatcaga 
      aacaaaaaa tccaagtac tacatctcg cctaatcag 
      tataaaccg gcggtcttt cccgacttt accaccggg 
      cccgcgtat ctgctgccc gcgcggctg tccaaagag 
      ctgtacgtg gcggcgctt aatcacacc tacttcaag 
      ctggaggat gtgttcgtt accggaatc gtggccaac 
      agcttgaag atcaagcgc gtgcacgcc ccggaattc 
      ctaaacaaa agagtctct ctaaccccg tgcagcgtg 
      cagaagggc atcagcata cacatggtc aaaggtgtg 
      gagcagtac gacctgtgg aagaagcta cacgacgtt 
      gccgcaaag tgcaagaaa tag
    mRNA sequence
    • >TC014213 RA
      tttgtagcc ataaaaata taacttttg tgagcattt 
      tctaacaaa ttatttggt ttcagtttt gacaccgtc 
      atgtcgtct gatgtgtta ccacatcga ttctcctac 
      agaacaaaa ataattttt tacaagtaa ttggcatgg 
      gggtgccat ggctcgccc aagtgcggt tgctgcaca 
      tggcgattt tagttctag cgacggcgt catgtctac 
      tgtacgtgg catatataa catcccccc aattgacga 
      caacagctt cacccctac gtacgttag tatcatccg 
      aaattcgcg cattccaag gtaacacga cccaggctg 
      aagtggcga agaacatga ccgtggcgc cgccttcct 
      cgaacagcg gcggctcgt ctgaagcgc cccaattgc 
      cagcagttc gcacgctga caaatgcga caaacagca 
      gtcagcccg acctaactc ggggagtcg ccgctgaga 
      taatatacg aggccggtc atgtggacg tcagttcgc 
      agatctgcc ccgagttag gcagagatt taaagttgt 
      tgattgcga taacgtcgg ctcccagcc acgaaagcg 
      cccgaatgg cgattcggg agacttggg gccacttcg 
      cgagcagaa aagacgtag ctatagcat ttatgttag 
      ggtcgattt cgaacgaga cggtcaacg ccaacatag 
      agaaggagc aatacttgt acggtgata taattcggg 
      ggaagttcc gagacacat atgacaatc ttactctta 
      aaacgattt cgatgttag aatgggtgg ataactact 
      gtccaaaag cagcttttg tacttaaaa ctgacgacg 
      acatgttta ttaacgtct cccggctgc tggctttca 
      ttgcaaagc acagtccgg agcaaagga cgatctatg 
      gccgcttgg ccaagaagt ggaaaccga tcagaaaca 
      aaaaatcca agtactaca tctcgccta atcagtata 
      aaccggcgg tctttcccg actttacca ccgggcccg 
      cgtatctgc tgcccgcgc ggctgtcca aagagctgt 
      acgtggcgg cgcttaatc acacctact tcaagctgg 
      aggatgtgt tcgttaccg gaatcgtgg ccaacagct 
      tgaagatca agcgcgtgc acgccccgg aattcctaa 
      acaaaagag tctctctaa ccccgtgca gcgtgcaga 
      agggcatca gcatacaca tggtcaaag gtgtggagc 
      agtacgacc tgtggaaga agctacacg acgttgccg 
      caaagtgca agaaatagt gcgtgatcg agacaattt 
      tttttcaat tgtgtacat tttcacaaa cagtatttt 
      acttaagtt aaattattg gtgaatctc atcaactgt 
      acaattctc acatgaaca tcacagttt atttgtact 
      gtaattaat tatcaatca aaaaaattt taaaattat 
      ataaataaa taagttgca taattagaa ctcattttt 
      atatcccct gaataaaca ttattatta ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY