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Gene information
General information
TC014210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3211348..3212624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014210
    iBeetle screen iB_05586

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 4

    vitellogenic egg chamber sometimes shape irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014210 RA
      MSGLRNPRP LVLCGPSGS GKSTLVKKM 
      MADFPDKFG FSISHTTRQ PRPGEVHGQ 
      HYHFTSREE MEQAISDGR FIESASFCG 
      NMYGTSKAA VEQVIQEGK VCVLDIDVQ 
      GVKQVKKTD LNPFYVFIK PPSLEELKN 
      RLTARKTES EESLNHRLK VASEEMEYG 
      TSDNFDFIV VNDNLDHAY AQLKTFVEE 
      KVLKETN
    CDS sequence
    • >TC014210 RA
      atgtctggc ttgcggaac ccccggccc ttggtgctg 
      tgcgggccc tcgggctcc ggcaaaagc acgctggtg 
      aagaagatg atggccgac ttcccggac aagttcggg 
      tttagcatc agtcacacg acccggcag ccccgtccg 
      ggggaggtg cacggccag cactaccac ttcacctct 
      cgggaggag atggagcag gccatcagc gacgggagg 
      ttcatcgaa agcgccagc ttttgcggg aacatgtac 
      gggacgagc aaagcggcg gtggagcaa gtgatccaa 
      gagggcaag gtctgtgtg ctggatatc gacgtccag 
      ggggtgaag caggtcaag aagacggac ctcaacccc 
      ttctacgtc ttcatcaag ccccccagt ttggaggag 
      ctgaagaac aggctcacg gcgcgcaaa accgagtcg 
      gaggagagt ctcaaccat aggttgaaa gtggccagt 
      gaggaaatg gaatatggg acttcggat aacttcgat 
      tttatcgta gttaatgat aatttagac cacgcatat 
      gctcagcta aagacgttt gtggaagaa aaggttttg 
      aaagagaca aattaa
    mRNA sequence
    • >TC014210 RA
      cttctaaaa ataatcaat agaagttcg tgcttcctc 
      cacctctca ttaggctaa ttagtgagc aaacacgaa 
      gacaccgcg gtcataatc ataaattag gaaaaccag 
      ccaaggcgc acccaaatt aattagccc agtccccaa 
      cacccgcca ttgatttaa atttagcgc gcggcacca 
      taaccacaa aaaacaaat ctccctgtg caaattgtt 
      ttgttgcgt gtcgggcgc ccagcacgt gtttcctcc 
      cgaaaaacc agcccttct ccaccattt ttctcctaa 
      gaggtttgg ccaggatgt ctggcttgc ggaaccccc 
      ggcccttgg tgctgtgcg ggccctcgg gctccggca 
      aaagcacgc tggtgaaga agatgatgg ccgacttcc 
      cggacaagt tcgggttta gcatcagtc acacgaccc 
      ggcagcccc gtccggggg aggtgcacg gccagcact 
      accacttca cctctcggg aggagatgg agcaggcca 
      tcagcgacg ggaggttca tcgaaagcg ccagctttt 
      gcgggaaca tgtacggga cgagcaaag cggcggtgg 
      agcaagtga tccaagagg gcaaggtct gtgtgctgg 
      atatcgacg tccaggggg tgaagcagg tcaagaaga 
      cggacctca accccttct acgtcttca tcaagcccc 
      ccagtttgg aggagctga agaacaggc tcacggcgc 
      gcaaaaccg agtcggagg agagtctca accataggt 
      tgaaagtgg ccagtgagg aaatggaat atgggactt 
      cggataact tcgatttta tcgtagtta atgataatt 
      tagaccacg catatgctc agctaaaga cgtttgtgg 
      aagaaaagg ttttgaaag agacaaatt aattgattt 
      agattttat taaaattac attccttgt ggagcattc 
      tggcctagt taattaaca aattgtact taatgtgta 
      gtcgttgct tcgcgttac ttgacatcg ttcttgttg 
      taaatagat gctagaagt aactgcatt tattgatag 
      ttaactgtg attttgagg tactttgag attgatttg 
      tacgaaact agagcgtcc aattaatgc tttattcgt 
      aatgaagca ataaaaaaa gcattgtat gtaaagttg 
      caataaatt gatgctcct aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY