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Gene information
General information
TC014209 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3205668..3207529
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014209
    iBeetle screen iB_05585

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014209 RA
      MLLSREFYT FLKSATSPA KLITLKNFH SCASYAAQV 
      SFKLSDIGE GIREVTVKE WFVKVGDKV SQFDEICEV 
      QSDKASVTI TSRYDGVIK KLHYKIDEI ASVGKPLVD 
      IETEGDEPS AAPTPEEES KPPVEEIKI SEPTDPQPT 
      AEILCIPSV RRLAKEHKV NLWEVTGTG KSGRILKED 
      VLKYLQAGP APAKAPARQ ARTEPIKGF QKAMVKTMS 
      DALKIPHFV YSDEIAVTQ LSQLRQTLK KLPETQDLK 
      LSFMPFFIK AASNALQRF PVLNASLDE NCENVTYKS 
      EHNIGVAMD TKVGLAVPV IKNVETLSI IEISNELNR 
      LIKSGRSGS FSPQDLAGG TFTISNIGA IGGTYMKPV 
      IMPPHVAIV ALGASQVVP RFDDAGNVV PVEVLNLSG 
      AADHRIIDG ATMARFVQT LKRQIENPY LLFLNL
    CDS sequence
    • >TC014209 RA
      atgttgctg tcaagggag ttttacact tttttaaaa 
      agtgcgacc agtccggca aaactaatc actttgaaa 
      aactttcac tcgtgtgcg agctatgcg gcccaagtg 
      tctttcaaa ctgtctgac attggtgaa ggcataagg 
      gaggtaaca gtcaaagag tggttcgtc aaagtcggc 
      gacaaagtc tcccaattt gacgaaatt tgcgaagta 
      cagagtgac aaagcctcg gtcacaatc acaagcaga 
      tacgatgga gttattaag aaactacat tacaaaatc 
      gacgagatt gcgtcagtt ggcaaaccc ctggtggac 
      attgaaacc gaaggggat gaaccaagc gcagcacca 
      acccccgaa gaagaatca aaaccccca gtcgaagaa 
      attaaaata agcgaaccc actgacccc caaccaacc 
      gctgaaatt ttatgcatc ccctctgtc cgaagactc 
      gccaaagaa cacaaggtg aacttatgg gaggtcaca 
      ggaaccgga aaatccggc cgcattctc aaagaagac 
      gttctgaaa tacctgcaa gccggccct gcgccggcc 
      aaggccccc gcccgccag gcccgcacc gagcccatc 
      aagggcttc cagaaggcc atggtcaag acaatgagc 
      gacgctctg aaaatccct cacttcgtc tacagcgac 
      gaaatcgca gtaacgcaa ctgtcgcaa ctgcgacaa 
      acccttaaa aaactgcca gaaacgcag gacttgaag 
      ctgtcgttc atgcccttc ttcatcaaa gcggcctcc 
      aacgccctg cagcgcttc ccggttttg aacgccagc 
      ttggacgag aactgcgag aatgtgacc tacaagagc 
      gagcacaac atcggggtt gcgatggac acgaaagtc 
      gggctggcc gtcccggtg ataaaaaac gtggagaca 
      cttagtatt attgaaatt agcaacgaa ctgaaccga 
      cttatcaag tcggggcgc agcggctcc ttctcgccc 
      caggacttg gccgggggc acgttcacg atttcgaac 
      attggggct atcggcggc acgtacatg aagccggtc 
      attatgccg ccgcacgtg gccattgtt gcgctcggg 
      gcgtcccag gtggttccc cggtttgac gacgccggg 
      aatgtcgtc cctgtcgaa gtgcttaat ctgagcggg 
      gccgccgac caccggatc atagacggg gccacgatg 
      gcccgcttt gtgcagact ttgaaacgg caaatcgaa 
      aacccctat ctcttgttc ctgaacttg tga
    mRNA sequence
    • >TC014209 RA
      aatttgaat aaatgtatt tgtgttttt gtggcaagt 
      cttaatctt tttgcaata atttactta accggttga 
      cctcaaaac gagttgatt cacctttgc ctctttctc 
      ttatcacgg agagataaa attcggata agttccacc 
      aaacgtagt gctctgtgt gtttttcga aaatgtgtt 
      aatcaacgt agcgggaaa tttaaaaat tgataatta 
      gtgattgag aaaaaacaa cgtcaatta tttaaatga 
      ctggacact ccgttactc aatacgacc atcacttgc 
      gttgataag cggccccaa gttgtattg ttatcagtc 
      gaaatttaa aataattaa aaacatgtt gctgtcaag 
      ggagtttta cactttttt aaaaagtgc gaccagtcc 
      ggcaaaact aatcacttt gaaaaactt tcactcgtg 
      tgcgagcta tgcggccca agtgtcttt caaactgtc 
      tgacattgg tgaaggcat aagggaggt aacagtcaa 
      agagtggtt cgtcaaagt cggcgacaa agtctccca 
      atttgacga aatttgcga agtacagag tgacaaagc 
      ctcggtcac aatcacaag cagatacga tggagttat 
      taagaaact acattacaa aatcgacga gattgcgtc 
      agttggcaa acccctggt ggacattga aaccgaagg 
      ggatgaacc aagcgcagc accaacccc cgaagaaga 
      atcaaaacc cccagtcga agaaattaa aataagcga 
      acccactga cccccaacc aaccgctga aattttatg 
      catcccctc tgtccgaag actcgccaa agaacacaa 
      ggtgaactt atgggaggt cacaggaac cggaaaatc 
      cggccgcat tctcaaaga agacgttct gaaatacct 
      gcaagccgg ccctgcgcc ggccaaggc ccccgcccg 
      ccaggcccg caccgagcc catcaaggg cttccagaa 
      ggccatggt caagacaat gagcgacgc tctgaaaat 
      ccctcactt cgtctacag cgacgaaat cgcagtaac 
      gcaactgtc gcaactgcg acaaaccct taaaaaact 
      gccagaaac gcaggactt gaagctgtc gttcatgcc 
      cttcttcat caaagcggc ctccaacgc cctgcagcg 
      cttcccggt tttgaacgc cagcttgga cgagaactg 
      cgagaatgt gacctacaa gagcgagca caacatcgg 
      ggttgcgat ggacacgaa agtcgggct ggccgtccc 
      ggtgataaa aaacgtgga gacacttag tattattga 
      aattagcaa cgaactgaa ccgacttat caagtcggg 
      gcgcagcgg ctccttctc gccccagga cttggccgg 
      gggcacgtt cacgatttc gaacattgg ggctatcgg 
      cggcacgta catgaagcc ggtcattat gccgccgca 
      cgtggccat tgttgcgct cggggcgtc ccaggtggt 
      tccccggtt tgacgacgc cgggaatgt cgtccctgt 
      cgaagtgct taatctgag cggggccgc cgaccaccg 
      gatcataga cggggccac gatggcccg ctttgtgca 
      gactttgaa acggcaaat cgaaaaccc ctatctctt 
      gttcctgaa cttgtgatg tgataaata aaacacgct 
      ttgcatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY