Status
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Gene information
General information
TC014196 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3027568..3039146
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014196
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05583

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014196 RA
      MASFRSIYE TFELRKPVS TLSIPGAVK NSSRDSAGG 
      RMQQEEPGV ALVGHVDYP RYMEDRTIG SGVYKGPSS 
      GSLKHKPNV GYRLGRRKA LFEKRKRIS DYALVMGMF 
      GIIVMVIEN ELSSAGVYR KDDFYSIAL KTLISVSTV 
      ILLGLIVAY HALEVQLFM IDNCADDWR IAMTWQRIC 
      QITMELVIC AVHPIPGHY RFVWTTKLS NHKDGGIGS 
      KCVPYDVPL SLPMFLRLY LICRVMLLH SKLFTDASS 
      RSIGALNRI NFNTRFVLK TLMTICPGT VLLVFMVSL 
      WIIASWTLR QCERFHDEE HANLLNAMW LIAITFLSV 
      GFGDIVPNT YCGRGIAVS TGIMGAGCT ALLVAVVSR 
      KLELTRAEK HVHNFMMDT QLTKRLKNA AANVLRETW 
      LIYKHTRLV KRVNPGRVR THQRKFLLA IYALRKVKM 
      DQRKLMDNA NTITDMAKT QNTVYEIVS DMSTRQDTL 
      EDRLTTMED KIVALQEQL DLLPDLIAT RIQAQAEKM 
      EQRRNFLHP ESAAGLQQA RSVPPGCPW PGPALAPAG 
      KQSAASSNA QS
    CDS sequence
    • >TC014196 RA
      atggcgtcg ttccgttcg atttatgag acctttgaa 
      ctacgaaag cccgtctcg actctctcc atccccgga 
      gcggtgaag aactcgagc agggactct gccggcgga 
      aggatgcag caggaggag ccgggagtc gccctcgtg 
      ggacacgtg gactacccc cggtacatg gaggacagg 
      actatagga agcggcgtc tacaaaggg ccttcgtct 
      ggaagtctc aagcacaaa cccaacgtg ggctatcgg 
      ttaggccgg cgcaaggcg ctttttgaa aagaggaaa 
      aggattagt gattacgct cttgttatg gggatgttt 
      gggattatt gttatggtt atagaaaat gaactctca 
      agtgctggc gtttatcgc aaagacgat ttttactca 
      attgcgctc aaaactctg atatctgtt tcgactgtg 
      attctttta ggcctcatt gtggcctat catgcttta 
      gaagtgcag ctttttatg atagacaac tgcgcggac 
      gattggcgg atcgcgatg acctggcag aggatctgc 
      caaatcacc atggaacta gtcatatgt gcagtgcat 
      ccgattcct ggacactat cggttcgtg tggacgact 
      aaattatcc aatcacaag gacgggggg atcggatca 
      aagtgtgta ccgtacgac gtgccctta tctttaccg 
      atgttcctt cggctttac ttaatctgc agagtgatg 
      ttactgcat agtaagttg ttcacggac gcatcgtcg 
      cgcagcata ggagctctt aatcgaatt aattttaat 
      acgcggttt gtcttaaaa actctcatg accatctgc 
      cccgggaca gtgctctta gtttttatg gtttccttg 
      tggattatc gccagttgg acattgcgc cagtgcgaa 
      aggttccac gatgaagaa cacgcaaac ctgcttaat 
      gctatgtgg ctgatagcg atcactttc ctgagcgtt 
      ggcttcgga gatattgtt cctaacacg tactgtgga 
      aggggcatt gcggtcagc acaggcatc atgggggct 
      ggttgcacg gccttactc gtggctgtg gtgtcgagg 
      aagctggaa ttgactcga gcggaaaag cacgtgcat 
      aattttatg atggacacc caactaaca aaacggcta 
      aaaaacgcc gccgccaac gttttacga gaaacttgg 
      ttaatttac aaacacaca aggctagtg aaaagggtg 
      aaccctggc agagttcgg actcatcaa agaaaattt 
      ttactcgca atttacgcg ttacgtaaa gttaaaatg 
      gaccaacgc aaattaatg gacaatgcc aacaccata 
      acggatatg gcgaagacg caaaacacc gtgtatgag 
      atagtttcc gatatgagc acgaggcaa gacactttg 
      gaagatcgc ttgacgacg atggaagat aagatcgta 
      gcacttcag gagcagcta gatttgcta cctgatctc 
      attgctact agaatccaa gctcaggcg gagaaaatg 
      gagcaaaga cgcaatttt ttgcatcct gaatccgcg 
      gctggtctt caacaggcg cgtagcgtg ccccctgga 
      tgcccttgg cccggtcct gctttagct cctgctgga 
      aagcaatct gctgcctct tcaaacgca cagagttga
    mRNA sequence
    • >TC014196 RA
      tatcagtcg ctccttgat tgtaggaaa gtttgtgaa 
      aaattttca ggcgtcaga tgaaatgtg cttttattg 
      tgtctctga aatgacgta cgcttatct gcgataatt 
      cgtcgccta aatgacaaa aatccgcga atgtaaacc 
      gggtgggtt tatctttat ggcccgctg tccatcggg 
      gctatctat gattatttt agtatgttt aggtttgat 
      ggctcgcag ttttgttcc tcctcttcg gaaaggaag 
      aggagcagc aaaagattt tttctgtta tcgatcgac 
      taatctggc gattggtcg cttgtttgt gtgtttgaa 
      cgaggtgtg agagtgata tttcgtaag atgagacgt 
      gcaatccca taatgtggt cctacgcag ggttcaggg 
      atgctgtcg tcctctcag accatggcg caagcgcgc 
      cgaactcga ctcaagcgc gctcttaca gaagtaacc 
      agcgatact gtgtcctat gtgaaaacg gtgggctca 
      tcaactaga gtccttttg actttaaag ctcgaaagt 
      taagctttt ttcacttaa cctcaaact gattcgacg 
      ctcccatgc gatcaatcg cggccttac tctaataaa 
      tgatgtgtt tgtttcgca aaactactg atatcactc 
      actgcaatt tgaactttt cgcaggaat gtgtcaaat 
      gagcaggta aacactcat ttaaatcat attcgcgcc 
      acgggccat caactcccg ctcttcgcc gctttcaaa 
      cttcactca ccattcatt attattttc aaacaagca 
      caatcgaat taaaataaa acaatattt ttgccaaaa 
      gcttggcac gaccaatga taaaaaagc gcaaagtaa 
      accacgtgt gcgcgcaac cgttgtaat ttgaattta 
      aaagactaa ttaatgcaa atccggcaa atgcgtgaa 
      tttgttttc tcgccgaat gggcgcttt taagatccg 
      gctttgacc gctaacgtg ccactagtt gtccataaa 
      gcgcccata acggccgat tttgtgctt tgttttatg 
      gaagtgcgt taaacatcc ataataccg ttgtgccgg 
      catagaatt cgcttccat ccattttac gtagttatg 
      cggaaattt atgtttgaa ttaaacaat ttataagcg 
      gcgcggcga agattaaac aaattcttt actcgggaa 
      aaggcggaa aagtgacaa aagagcgaa cgatgccag 
      ataacattt ttacaattc gagcggatg ggaagtcgg 
      aaagcgggt ctatgtagg gaatcaatg ggaattttt 
      atatatgcg attattacc tcccttggt ggttttaaa 
      acgggacgt taaatgtcg gaatattat ccagcgtta 
      ttgcaaggg gtccgcaaa tgcgggaat ttacttttc 
      accgtcgat tcgacgcac gtgatttgg cattttgag 
      aataaaata aaatttttg aaaaaggac aatatctgg 
      taatgcctc ctctaatta attttactg taggtttta 
      ggaacagtt gggcgaccc gtcccttta tcacctgat 
      aaagtggac cacgtggtt atcagccca tcgccaatt 
      attcataac tttctttgc aagtcttat cacaaagtt 
      tcctctttt atctatctt ttgacctta aaattgcat 
      gtaaaccaa gcaattaga gaagttcac aagtttaca 
      acttgcatg atttgttac cccgacaat taccaattg 
      cactttcca cggtgtaat tcaaagtaa aagattgta 
      cccgttgtt taggacatg gaaaatccg agctttcag 
      aaaaccaat cctgtccaa acaccgtga ttgtgactt 
      tttattata gatttccgt gattctttt actattttt 
      tcgaaccgg atttgcatg taactcgat tctgtgtga 
      tagacacgt tctgatttt caactatcc gtctttgtt 
      ccttattga ttatctctt atctttttc acacttcct 
      acaacacac gttttcgca ccaattatt ttcctaatg 
      ccagaacat aattgcttt aagtttttt taatcaaga 
      tcccgaaac aaagattta ttgtgatgc taacagcaa 
      aattaatat cggatcaga atcaagatg aagtattgt 
      cttgtaata attaaagtt gtggttaga aaccggaaa 
      aaccggttt atttcgaac acaaacata atactgcag 
      cagctcaat caaaaccgg aaaaaccgg ttcgcttct 
      aaataaaac gaaacataa aaagaaaag tagacgaaa 
      cacaaagaa gtcgatcca accctaaga taaatttat 
      tatgtgaag ttcaacaaa ctcatctct cgaaatggc 
      attcagata tgatccgag agaatcagt tcaaagtga 
      aaattcgtt tatttaatc cattttcat gttaattac 
      aaaaaacac tcgtgacgt ttcagggaa actcggccg 
      ttgtttata ttttcgttt cactttctg gattgtttt 
      gacatgtgt ataatttct tttgtgcag gatatcagt 
      agcgaatgt gcactcgtt ttgaatttg attaagttg 
      aaaccatca agcagtcct taatgaaaa tttcatgta 
      ttattacag cgacttgcg gttcctgct aggcatcaa 
      attaaatta attcgttta gttggaaat tttcgtgca 
      agcacgcag ccactcccg gccgaacta ccaatgaag 
      ttttttaat ttcaaaaaa ttgcctaag caaattttc 
      aactattac tcaaaactc cgacaaggg tcactctca 
      ctcaccgca ataactaat tattgtgaa caacataat 
      caatcacta aatagattt tactcgaaa gaattctga 
      taaacacgc acattatta cattaattg cccaaaaag 
      caattgtga tttatagtt caatttgtg cacgactcc 
      gaaagctga aatatgctg agaagtgag aaaagttga 
      aaacccgaa ttattatcc tcgacaagg aataatagt 
      gcactaaaa accgtttat ttaattttg ctaaaagta 
      tatccaata caaaaatgc aaaaaacac gcgtaacaa 
      aacttaata ctaagtttt ttaactgag tccggattc 
      cagaagaat tggatgttt tgtgaatat cactcaaga 
      aaatggttt ggaaaagca caactcttt gatcacctc 
      ggcttaaaa gctttccag cttttagct tacaaaaat 
      caaatttct gcgttaata atgcatgtg tgtgtgaat 
      gaaatgtca aggaaaagt cgtttcaca caactttaa 
      ataaataat gtttggtag tgacagcca actgaaagg 
      aaaatctgt gagaaagta atcttcaaa ttgcattat 
      caaaaattc tgtgaattt atttagctt tgctaagtt 
      attagagta gatgcgaac gcggattgc ttgaaaaca 
      ttttgatgt acagttgca aatgagtgg actccaact 
      agctgtcaa gttcattaa aaatatgtc gagggttga 
      gggtggaaa ttttaatta tgcaaataa tgagaagtg 
      atttttctg acattttcc gagcggtaa tgtaaaaaa 
      tggcgtcgt tccgttcga tttatgaga cctttgaac 
      tacgaaagc ccgtctcga ctctctcca tccccggag 
      cggtgaaga actcgagca gggactctg ccggcggaa 
      ggatgcagc aggaggagc cgggagtcg ccctcgtgg 
      gacacgtgg actaccccc ggtacatgg aggacagga 
      ctataggaa gcggcgtct acaaagggc cttcgtctg 
      gaagtctca agcacaaac ccaacgtgg gctatcggt 
      taggccggc gcaaggcgc tttttgaaa agaggaaaa 
      ggattagtg attacgctc ttgttatgg ggatgtttg 
      ggattattg ttatggtta tagaaaatg aactctcaa 
      gtgctggcg tttatcgca aagacgatt tttactcaa 
      ttgcgctca aaactctga tatctgttt cgactgtga 
      ttcttttag gcctcattg tggcctatc atgctttag 
      aagtgcagc tttttatga tagacaact gcgcggacg 
      attggcgga tcgcgatga cctggcaga ggatctgcc 
      aaatcacca tggaactag tcatatgtg cagtgcatc 
      cgattcctg gacactatc ggttcgtgt ggacgacta 
      aattatcca atcacaagg acgggggga tcggatcaa 
      agtgtgtac cgtacgacg tgcccttat ctttaccga 
      tgttccttc ggctttact taatctgca gagtgatgt 
      tactgcata gtaagttgt tcacggacg catcgtcgc 
      gcagcatag gagctctta atcgaatta attttaata 
      cgcggtttg tcttaaaaa ctctcatga ccatctgcc 
      ccgggacag tgctcttag tttttatgg tttccttgt 
      ggattatcg ccagttgga cattgcgcc agtgcgaaa 
      ggttccacg atgaagaac acgcaaacc tgcttaatg 
      ctatgtggc tgatagcga tcactttcc tgagcgttg 
      gcttcggag atattgttc ctaacacgt actgtggaa 
      ggggcattg cggtcagca caggcatca tgggggctg 
      gttgcacgg ccttactcg tggctgtgg tgtcgagga 
      agctggaat tgactcgag cggaaaagc acgtgcata 
      attttatga tggacaccc aactaacaa aacggctaa 
      aaaacgccg ccgccaacg ttttacgag aaacttggt 
      taatttaca aacacacaa ggctagtga aaagggtga 
      accctggca gagttcgga ctcatcaaa gaaaatttt 
      tactcgcaa tttacgcgt tacgtaaag ttaaaatgg 
      accaacgca aattaatgg acaatgcca acaccataa 
      cggatatgg cgaagacgc aaaacaccg tgtatgaga 
      tagtttccg atatgagca cgaggcaag acactttgg 
      aagatcgct tgacgacga tggaagata agatcgtag 
      cacttcagg agcagctag atttgctac ctgatctca 
      ttgctacta gaatccaag ctcaggcgg agaaaatgg 
      agcaaagac gcaattttt tgcatcctg aatccgcgg 
      ctggtcttc aacaggcgc gtagcgtgc cccctggat 
      gcccttggc ccggtcctg ctttagctc ctgctggaa 
      agcaatctg ctgcctctt caaacgcac agagttgat 
      ttccttatt gggattaac tatgcaaca cagtattta 
      gtcggtata tatatttta atgaacatc atggctaga 
      actagtttc ggattcaga tttactcat ttctggcct 
      atttcgagc ggactcacc taatttaca aaaccatga 
      tgaacaata ctaaatata tattgagaa gttagagcc 
      ttatctata tacagtatt agtaataat acaaccacg 
      ttgatacta ataggcgtt agttactca aacgatttc 
      atgtcatcg atcataaaa tactataat catatctca 
      gccaatagt tagccaact ccaaattta caaaacact 
      ctaaccaac cattaatgt gccaacgaa cgacattaa 
      gttttcttg taccatact aacgctatt ttcagactt 
      ttatacgag atatgataa agtagtttt ttaataaat 
      ctattttga agtctgatc tagacgcga gagaccatc 
      acttcctga aaccattgg tctagttat gcaatgtgt 
      tggtctgac attttcaga tttcaattt atattttta 
      ttcattctt cagagctat caaattagt ttagtctca 
      ttctgccac attcacacg aaattggcg taattagga 
      gatgtggta gagtgcaaa tccgcaaat ttgctaatt 
      catttttca tcaaacttc agccaaatt taggctatt 
      agttaatta taaattcca ttagaaagc ttcgttaaa 
      tgctttaat tccagtaat atgtaatga cttgactga 
      ctgaagttt tagatgtag tacgctata tgattatag 
      tattttgtt aaaaatcta taaaccaac gataagcac 
      atttacttt gtgaataca caattcatg gattccctt 
      aacacaata gtaatcgcc atttctaac tttttttaa 
      tctaattat atgataagc ctgaaacag tcctcagta 
      acggttcta tcgtagcgt atcattatg taacaacat 
      tttgtttaa actgtgctc gtggaaggc ttcggccgt 
      gtaacttgt tatacatag tttctaaac aatacatca 
      actagtgca tttttaaac aatgagtac ttccacaat 
      tgacataaa gtatattat tattgataa acatatttc 
      ttattcagg catttcgca ccgattgta ccatacttc 
      atacttttt taaatctta gccttcatt tctttcaac 
      tcgttcagt ttgtatata gctctgtaa atactggat 
      aagaaatat gagtgtgaa agtaaagct ttagatttg 
      ataaatcag tgaaaatac tggtgtgat agtgtatgg 
      tgcacctta agcattata tattcagag gtccaaatt 
      gttaaactg ttagagttt tctgcaatt tcacaagtt 
      ttataagca tatattata taatttagt atataccct 
      atattatac aagacgtat atcagcata gttagcctg 
      ttttaaatt tcttagcgt tatatattt tcaagtgca 
      ttttcatgt taccctact catgttacc aacgaataa 
      actactagt ttccta
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