Status
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Gene information
General information
TC031499
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014191
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2976750..2993034
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031499
    iBeetle screen iB_05582

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 48
    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031499 RA
      MQDEDKAGS GEEAPEKGK EDAELEKLE AASTKAAAS 
      LPLFHPAEG GAPALWSAA VEDSYAPMS VNGLGFQNF 
      PSPNQQQLF GNNQSQGRR AITASHNFP HMGRQSGHP 
      LYKGYGSWA SPGQTSWSG GSSAWNRGR SVPNLTPLQ 
      QMGVAGRKP SPTFNHQQV ISPVKFRRS TSYPGKGPF 
      PQPAPTFEI TNMDEARDL LPYQDRCGV VNANGSSPL 
      ENMRGLEHY LNDIMRPPQ DSTDIKGFI NCNTNAFQS 
      LTQLHGKSS YFQVLDDQP GPVLLEEPH LVDSTLGGA 
      QPLSSPSRS SPHSQGSEG GERFSRKVF VGGLPPDID 
      EDEITASFR RFGPLVVDW PHKAESKSY FPPKGYAFL 
      LFQDENSVQ SLIDACITD EDKLYLCVS SPTIKDKPV 
      QIRPWRLSD ADFVLDASM PLDPRKTVF VGGVPRPLK 
      AVELAMIMD RLYGGVCYA GIDTDPELK YPKGAGRVA 
      FSNQQSYIA AISARFVQL QHGDIDKRV EVKPYVLDD 
      QMCDECQGQ RCGGKFAPF FCANVTCLQ YYCEHCWAT 
      IHSRPGREF HKPLVKEGA DRPRAVPFR WC
    • >TC031499 RB
      MQDEDKAGS GEEAPEKGK EDAELEKLE AASTKAAAS 
      LPLFHPAEG GAPALWSAA VEDSYAPMS VNGLGFQNF 
      PSPNQQQLF GNNQSQGRR AITASHNFP HMGRQSGHP 
      LYKGYGSWA SPGQTSWSG GSSAWNRGR SVPNLTPLQ 
      QMGVAGRKP SPTFNHQQV ISPVKFRRS TSYPGKGPF 
      PQPAPTFEI TNMDEARDL LPYQDRCGV VNANGSSPL 
      ENMRGLEHY LNDIMRPPQ DSTDIKGKE CYVEKVSCA 
      MFRAYGTSP VDTSAPTPT WGLQFNSIS KGFINCNTN 
      AFQSLTQLH GKSSYFQVL DDQPGPVLL EEPHLVDST 
      LGGAQPLSS PSRSSPHSQ GSEGGERFS RKVFVGGLP 
      PDIDEDEIT ASFRRFGPL VVDWPHKAE SKSYFPPKG 
      YAFLLFQDE NSVQSLIDA CITDEDKLY LCVSSPTIK 
      DKPVQIRPW RLSDADFVL DASMPLDPR KTVFVGGVP 
      RPLKAVELA MIMDRLYGG VCYAGIDTD PELKYPKGA 
      GRVAFSNQQ SYIAAISAR FVQLQHGDI DKRVEVKPY 
      VLDDQMCDE CQGQRCGGK FAPFFCANV TCLQYYCEH 
      CWATIHSRP GREFHKPLV KEGADRPRA VPFRWC
    CDS sequence
    • >TC031499 RA
      atgcaggat gaggacaag gccggcagc ggcgaggag 
      gcccccgag aagggcaag gaggacgcc gagctcgag 
      aagctggag gcggcgtcc acgaaggcc gcggcctcg 
      ctgccgctc ttccacccc gccgagggg ggcgccccg 
      gcgctgtgg tcggcggcc gtcgaggac tcgtacgcc 
      cccatgagc gtcaacggc ctcggcttc cagaacttc 
      ccgtccccg aaccagcag cagctgttc ggcaacaac 
      cagagccag ggccggcgg gccatcacg gccagccac 
      aacttcccg cacatgggc cgccagtcc ggccacccg 
      ctctacaag ggctacggc tcgtgggcc agcccgggc 
      cagacgagc tggtcgggg ggctcctcg gcctggaac 
      cgcggccgc agcgtgccc aacctcacg ccgctccag 
      cagatgggg gtcgccggc cggaagccc agtcccacc 
      ttcaaccac caacaagtc atatctcca gtcaaattc 
      agacgcagc acctcctat ccgggcaaa gggcccttt 
      ccccagccc gctcccacc ttcgaaatc accaacatg 
      gatgaggcc agagatctg cttccctat caggaccgg 
      tgtggtgta gtgaacgcg aatggaagt agtccttta 
      gaaaatatg agaggccta gaacattat ttgaatgat 
      ataatgaga ccacctcaa gactcaacg gatatcaaa 
      gggtttatc aactgcaat accaatgct ttccagtca 
      ctgacacag ttacatggg aaatcgtct tacttccaa 
      gttttagat gaccagccc gggcctgta ctgctcgaa 
      gagccgcac ttagtggat agtacgtta ggtggtgcc 
      cagcctctg agttcccca tcgaggtct tcgccgcac 
      tcgcagggc tcggaaggc ggggagaga ttttcgcgt 
      aaagtattc gtcgggggc ctgccaccg gacatagat 
      gaagacgaa atcacggct agtttccga cgattcgga 
      cccctcgtc gtcgactgg ccccacaag gccgaaagc 
      aaatcgtat ttccccccg aaaggatat gcattccta 
      ttattccag gatgaaaat tctgtacaa agtctaata 
      gatgcgtgc attacggat gaagataaa ctgtatctt 
      tgtgtatcg tcgccaaca atcaaagac aagcctgta 
      cagattaga ccatggagg ttgtcggat gcggatttc 
      gttttagat gcttctatg ccgttggac cctcggaag 
      accgtcttc gtcggcggg gttccacgt cccctgaaa 
      gctgtggaa ttagctatg ataatggat cgtctctat 
      ggtggggtg tgttatgct ggcattgac actgaccca 
      gagctcaag tatccaaaa ggtgcagga cgagtagcg 
      tttagtaat cagcagagt tatatcgct gcaattagt 
      gcacgtttt gtccaactg caacacggt gatattgac 
      aaaagggtg gaagtgaaa ccttacgtc ctcgacgat 
      cagatgtgt gatgaatgt caaggacag cgttgtggt 
      ggcaaattt gcacctttc ttctgtgct aacgtgact 
      tgtttgcag tactattgc gaacactgc tgggcgaca 
      attcactcg aggcctgga cgagagttt cataagccc 
      ttggtaaag gagggtgcg gatcgacca agagctgtt 
      cctttccgc tggtgttaa
    • >TC031499 RB
      atgcaggat gaggacaag gccggcagc ggcgaggag 
      gcccccgag aagggcaag gaggacgcc gagctcgag 
      aagctggag gcggcgtcc acgaaggcc gcggcctcg 
      ctgccgctc ttccacccc gccgagggg ggcgccccg 
      gcgctgtgg tcggcggcc gtcgaggac tcgtacgcc 
      cccatgagc gtcaacggc ctcggcttc cagaacttc 
      ccgtccccg aaccagcag cagctgttc ggcaacaac 
      cagagccag ggccggcgg gccatcacg gccagccac 
      aacttcccg cacatgggc cgccagtcc ggccacccg 
      ctctacaag ggctacggc tcgtgggcc agcccgggc 
      cagacgagc tggtcgggg ggctcctcg gcctggaac 
      cgcggccgc agcgtgccc aacctcacg ccgctccag 
      cagatgggg gtcgccggc cggaagccc agtcccacc 
      ttcaaccac caacaagtc atatctcca gtcaaattc 
      agacgcagc acctcctat ccgggcaaa gggcccttt 
      ccccagccc gctcccacc ttcgaaatc accaacatg 
      gatgaggcc agagatctg cttccctat caggaccgg 
      tgtggtgta gtgaacgcg aatggaagt agtccttta 
      gaaaatatg agaggccta gaacattat ttgaatgat 
      ataatgaga ccacctcaa gactcaacg gatatcaaa 
      ggtaaagaa tgttacgta gaaaaagtg tcatgtgct 
      atgttcagg gcttacgga acttcacct gtggacacc 
      tcggcccct acacctaca tggggatta cagttcaac 
      tccatttct aaagggttt atcaactgc aataccaat 
      gctttccag tcactgaca cagttacat gggaaatcg 
      tcttacttc caagtttta gatgaccag cccgggcct 
      gtactgctc gaagagccg cacttagtg gatagtacg 
      ttaggtggt gcccagcct ctgagttcc ccatcgagg 
      tcttcgccg cactcgcag ggctcggaa ggcggggag 
      agattttcg cgtaaagta ttcgtcggg ggcctgcca 
      ccggacata gatgaagac gaaatcacg gctagtttc 
      cgacgattc ggacccctc gtcgtcgac tggccccac 
      aaggccgaa agcaaatcg tatttcccc ccgaaagga 
      tatgcattc ctattattc caggatgaa aattctgta 
      caaagtcta atagatgcg tgcattacg gatgaagat 
      aaactgtat ctttgtgta tcgtcgcca acaatcaaa 
      gacaagcct gtacagatt agaccatgg aggttgtcg 
      gatgcggat ttcgtttta gatgcttct atgccgttg 
      gaccctcgg aagaccgtc ttcgtcggc ggggttcca 
      cgtcccctg aaagctgtg gaattagct atgataatg 
      gatcgtctc tatggtggg gtgtgttat gctggcatt 
      gacactgac ccagagctc aagtatcca aaaggtgca 
      ggacgagta gcgtttagt aatcagcag agttatatc 
      gctgcaatt agtgcacgt tttgtccaa ctgcaacac 
      ggtgatatt gacaaaagg gtggaagtg aaaccttac 
      gtcctcgac gatcagatg tgtgatgaa tgtcaagga 
      cagcgttgt ggtggcaaa tttgcacct ttcttctgt 
      gctaacgtg acttgtttg cagtactat tgcgaacac 
      tgctgggcg acaattcac tcgaggcct ggacgagag 
      tttcataag cccttggta aaggagggt gcggatcga 
      ccaagagct gttcctttc cgctggtgt taa
    mRNA sequence
    • >TC031499 RA
      nnnaacgag gataaccgg ccctagaca gagtgacgt 
      gcactaatg ggggagttc agtttgagt ggcaacgcg 
      aaacatcgg tttgagtgc tgtggagat ccccgtgaa 
      cacgtcgaa gagcagcac gatcttcta caccacacc 
      tacgtcgac gtcgacgag cggcagcga ctcctccgt 
      cctccaggc cgcgagccg gccgatgca ggatgagga 
      caaggccgg cagcggcga ggaggcccc cgagaaggg 
      caaggagga cgccgagct cgagaagct ggaggcggc 
      gtccacgaa ggccgcggc ctcgctgcc gctcttcca 
      ccccgccga ggggggcgc cccggcgct gtggtcggc 
      ggccgtcga ggactcgta cgcccccat gagcgtcaa 
      cggcctcgg cttccagaa cttcccgtc cccgaacca 
      gcagcagct gttcggcaa caaccagag ccagggccg 
      gcgggccat cacggccag ccacaactt cccgcacat 
      gggccgcca gtccggcca cccgctcta caagggcta 
      cggctcgtg ggccagccc gggccagac gagctggtc 
      ggggggctc ctcggcctg gaaccgcgg ccgcagcgt 
      gcccaacct cacgccgct ccagcagat gggggtcgc 
      cggccggaa gcccagtcc caccttcaa ccaccaaca 
      agtcatatc tccagtcaa attcagacg cagcacctc 
      ctatccggg caaagggcc ctttcccca gcccgctcc 
      caccttcga aatcaccaa catggatga ggccagaga 
      tctgcttcc ctatcagga ccggtgtgg tgtagtgaa 
      cgcgaatgg aagtagtcc tttagaaaa tatgagagg 
      cctagaaca ttatttgaa tgatataat gagaccacc 
      tcaagactc aacggatat caaagggtt tatcaactg 
      caataccaa tgctttcca gtcactgac acagttaca 
      tgggaaatc gtcttactt ccaagtttt agatgacca 
      gcccgggcc tgtactgct cgaagagcc gcacttagt 
      ggatagtac gttaggtgg tgcccagcc tctgagttc 
      cccatcgag gtcttcgcc gcactcgca gggctcgga 
      aggcgggga gagattttc gcgtaaagt attcgtcgg 
      gggcctgcc accggacat agatgaaga cgaaatcac 
      ggctagttt ccgacgatt cggacccct cgtcgtcga 
      ctggcccca caaggccga aagcaaatc gtatttccc 
      cccgaaagg atatgcatt cctattatt ccaggatga 
      aaattctgt acaaagtct aatagatgc gtgcattac 
      ggatgaaga taaactgta tctttgtgt atcgtcgcc 
      aacaatcaa agacaagcc tgtacagat tagaccatg 
      gaggttgtc ggatgcgga tttcgtttt agatgcttc 
      tatgccgtt ggaccctcg gaagaccgt cttcgtcgg 
      cggggttcc acgtcccct gaaagctgt ggaattagc 
      tatgataat ggatcgtct ctatggtgg ggtgtgtta 
      tgctggcat tgacactga cccagagct caagtatcc 
      aaaaggtgc aggacgagt agcgtttag taatcagca 
      gagttatat cgctgcaat tagtgcacg ttttgtcca 
      actgcaaca cggtgatat tgacaaaag ggtggaagt 
      gaaacctta cgtcctcga cgatcagat gtgtgatga 
      atgtcaagg acagcgttg tggtggcaa atttgcacc 
      tttcttctg tgctaacgt gacttgttt gcagtacta 
      ttgcgaaca ctgctgggc gacaattca ctcgaggcc 
      tggacgaga gtttcataa gcccttggt aaaggaggg 
      tgcggatcg accaagagc tgttccttt ccgctggtg 
      ttaacgcag gagcgctta gtcagcggc cgccacttt 
      tagcttatt accaccggc ggcacggct gtccatcgc 
      ataggcata attatctag tagcaatta tacatattt 
      aaagtatac atgtaacca gttatcttc tgtctgagg 
      cgcctggtg gtagcggat tccactgca ccagcacca 
      gccgatgcg tctgcgttc agccattgt ccgactacg 
      atgtgtatc gctgtattt cgagacgag aagcctcct 
      tttggttct atgtctagt tcttgtagg taatcaaaa 
      aaagcaaaa aataaaata aatagtaga gaaaaatcg 
      gggaaggaa ccaagaggt acttctgca tatgatgtt 
      tagtgcggt atagtcaga aactttcag ggtattgta 
      aatactagt ctaatctgc aagccgctg ctgggcaga 
      ggatcttcg cttgcggcg accacttac cacttagcc 
      cccaagtac ctgaccggc ccgcctttg cccgtgtcc 
      cgctattaa gccccttta ataacacta acccacctg 
      gctttcccg ctgccccgc aaacctgct gctttatgt 
      ccgcactcc taccatcct ttattttaa tgtgtgata 
      ccataaacg tctttatgt attaaatga attgcacgg 
      atatgtata gtatatatt atatatagt atcagctat 
      tctaagact ttgtatttt ttaaatttg aatttttat 
      actaaactg ttataaatg gtgattagc aaaagtttg 
      atgtgtgca ataattgcg cttggggta gcccagctg 
      acctcggcg ccgtagagt tttatcttt aaccgtaat 
      taatgaata tataacgtt tcctaggta attgtgtaa 
      ataggcgcg gggcgatga gattgcagg ccgtacccc 
      taaacgtct agtcagggt gcagccacc cggcccgtg 
      cgaaccatc tacaatatg gtacaaata tgtattgat 
      atgatttgt aaagattta cactccttt ttctactgt 
      ttgtatatt tgctcgcct gccctgtgg ggagtagat 
      gatagttga aacggacaa gaccgtatc atattctgt 
      agacagttg tcattttga tctaacgat tacataact 
      ttttatccg tttttgaga tttagaagt gtgtgcagt 
      cagattgtt tcgaaactg attgtatca ttttcatat 
      ttttactat gtaagctgt gtatttatt ggaaaattt 
      ttaaagttg tatacattt tttactttt tataactgt 
      tttcaagca aattttttg agttattat ttactattt 
      tatatgtgg atatggaca gtcacaact gcgaccagt 
      ctgaaatga aactggcaa caagtactg tgtttccag 
      tttgattgt agttaccat tagtgcaat acatcgggc 
      tgttgcctc gattaggga tagtcaatt gtttcagat 
      ccttattgt tgcccaaat ggatctttc agttttgcc 
      cggtttgtt gaccgttgg ctacttgac tagcaacaa 
      actagtttt ttattatta ttctaacta ttattatta 
      tttttttgt attttacag aatactatt taaattgtt 
      tttataatt attttatac acttttgta tgtttgtta 
      gtcaatttg agatagggt ttgtaaaac tgtgtataa 
      acagtttct cctttaaga ttttgttaa atat
    • >TC031499 RB
      nnnaacgag gataaccgg ccctagaca gagtgacgt 
      gcactaatg ggggagttc agtttgagt ggcaacgcg 
      aaacatcgg tttgagtgc tgtggagat ccccgtgaa 
      cacgtcgaa gagcagcac gatcttcta caccacacc 
      tacgtcgac gtcgacgag cggcagcga ctcctccgt 
      cctccaggc cgcgagccg gccgatgca ggatgagga 
      caaggccgg cagcggcga ggaggcccc cgagaaggg 
      caaggagga cgccgagct cgagaagct ggaggcggc 
      gtccacgaa ggccgcggc ctcgctgcc gctcttcca 
      ccccgccga ggggggcgc cccggcgct gtggtcggc 
      ggccgtcga ggactcgta cgcccccat gagcgtcaa 
      cggcctcgg cttccagaa cttcccgtc cccgaacca 
      gcagcagct gttcggcaa caaccagag ccagggccg 
      gcgggccat cacggccag ccacaactt cccgcacat 
      gggccgcca gtccggcca cccgctcta caagggcta 
      cggctcgtg ggccagccc gggccagac gagctggtc 
      ggggggctc ctcggcctg gaaccgcgg ccgcagcgt 
      gcccaacct cacgccgct ccagcagat gggggtcgc 
      cggccggaa gcccagtcc caccttcaa ccaccaaca 
      agtcatatc tccagtcaa attcagacg cagcacctc 
      ctatccggg caaagggcc ctttcccca gcccgctcc 
      caccttcga aatcaccaa catggatga ggccagaga 
      tctgcttcc ctatcagga ccggtgtgg tgtagtgaa 
      cgcgaatgg aagtagtcc tttagaaaa tatgagagg 
      cctagaaca ttatttgaa tgatataat gagaccacc 
      tcaagactc aacggatat caaaggtaa agaatgtta 
      cgtagaaaa agtgtcatg tgctatgtt cagggctta 
      cggaacttc acctgtgga cacctcggc ccctacacc 
      tacatgggg attacagtt caactccat ttctaaagg 
      gtttatcaa ctgcaatac caatgcttt ccagtcact 
      gacacagtt acatgggaa atcgtctta cttccaagt 
      tttagatga ccagcccgg gcctgtact gctcgaaga 
      gccgcactt agtggatag tacgttagg tggtgccca 
      gcctctgag ttccccatc gaggtcttc gccgcactc 
      gcagggctc ggaaggcgg ggagagatt ttcgcgtaa 
      agtattcgt cgggggcct gccaccgga catagatga 
      agacgaaat cacggctag tttccgacg attcggacc 
      cctcgtcgt cgactggcc ccacaaggc cgaaagcaa 
      atcgtattt ccccccgaa aggatatgc attcctatt 
      attccagga tgaaaattc tgtacaaag tctaataga 
      tgcgtgcat tacggatga agataaact gtatctttg 
      tgtatcgtc gccaacaat caaagacaa gcctgtaca 
      gattagacc atggaggtt gtcggatgc ggatttcgt 
      tttagatgc ttctatgcc gttggaccc tcggaagac 
      cgtcttcgt cggcggggt tccacgtcc cctgaaagc 
      tgtggaatt agctatgat aatggatcg tctctatgg 
      tggggtgtg ttatgctgg cattgacac tgacccaga 
      gctcaagta tccaaaagg tgcaggacg agtagcgtt 
      tagtaatca gcagagtta tatcgctgc aattagtgc 
      acgttttgt ccaactgca acacggtga tattgacaa 
      aagggtgga agtgaaacc ttacgtcct cgacgatca 
      gatgtgtga tgaatgtca aggacagcg ttgtggtgg 
      caaatttgc acctttctt ctgtgctaa cgtgacttg 
      tttgcagta ctattgcga acactgctg ggcgacaat 
      tcactcgag gcctggacg agagtttca taagccctt 
      ggtaaagga gggtgcgga tcgaccaag agctgttcc 
      tttccgctg gtgttaacg caggagcgc ttagtcagc 
      ggccgccac ttttagctt attaccacc ggcggcacg 
      gctgtccat cgcataggc ataattatc tagtagcaa 
      ttatacata tttaaagta tacatgtaa ccagttatc 
      ttctgtctg aggcgcctg gtggtagcg gattccact 
      gcaccagca ccagccgat gcgtctgcg ttcagccat 
      tgtccgact acgatgtgt atcgctgta tttcgagac 
      gagaagcct ccttttggt tctatgtct agttcttgt 
      aggtaatca aaaaaagca aaaaataaa ataaatagt 
      agagaaaaa tcggggaag gaaccaaga ggtacttct 
      gcatatgat gtttagtgc ggtatagtc agaaacttt 
      cagggtatt gtaaatact agtctaatc tgcaagccg 
      ctgctgggc agaggatct tcgcttgcg gcgaccact 
      taccactta gcccccaag tacctgacc ggcccgcct 
      ttgcccgtg tcccgctat taagcccct ttaataaca 
      ctaacccac ctggctttc ccgctgccc cgcaaacct 
      gctgcttta tgtccgcac tcctaccat cctttattt 
      taatgtgtg ataccataa acgtcttta tgtattaaa 
      tgaattgca cggatatgt atagtatat attatatat 
      agtatcagc tattctaag actttgtat tttttaaat 
      ttgaatttt tatactaaa ctgttataa atggtgatt 
      agcaaaagt ttgatgtgt gcaataatt gcgcttggg 
      gtagcccag ctgacctcg gcgccgtag agttttatc 
      tttaaccgt aattaatga atatataac gtttcctag 
      gtaattgtg taaataggc gcggggcga tgagattgc 
      aggccgtac ccctaaacg tctagtcag ggtgcagcc 
      acccggccc gtgcgaacc atctacaat atggtacaa 
      atatgtatt gatatgatt tgtaaagat ttacactcc 
      tttttctac tgtttgtat atttgctcg cctgccctg 
      tggggagta gatgatagt tgaaacgga caagaccgt 
      atcatattc tgtagacag ttgtcattt tgatctaac 
      gattacata actttttat ccgtttttg agatttaga 
      agtgtgtgc agtcagatt gtttcgaaa ctgattgta 
      tcattttca tatttttac tatgtaagc tgtgtattt 
      attggaaaa tttttaaag ttgtataca ttttttact 
      ttttataac tgttttcaa gcaaatttt ttgagttat 
      tatttacta ttttatatg tggatatgg acagtcaca 
      actgcgacc agtctgaaa tgaaactgg caacaagta 
      ctgtgtttc cagtttgat tgtagttac cattagtgc 
      aatacatcg ggctgttgc ctcgattag ggatagtca 
      attgtttca gatccttat tgttgccca aatggatct 
      ttcagtttt gcccggttt gttgaccgt tggctactt 
      gactagcaa caaactagt tttttatta ttattctaa 
      ctattatta ttatttttt tgtatttta cagaatact 
      atttaaatt gtttttata attatttta tacactttt 
      gtatgtttg ttagtcaat ttgagatag ggtttgtaa 
      aactgtgta taaacagtt tctccttta agattttgt 
      taaatat
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