Status
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Gene information
General information
TC031495
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014183,TC014182
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2858286..2862865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031495
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05580

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031495 RA
      MEQKNSVTE VAGMSNGYR RPRTSADFY DFCTVVLQY 
      ANYEGWKKD DLRNETNQS PPLDSSGSS VNSESTSSG 
      QKDKQSEDD EDSDSFNLV TCFCGKPFA GRPMIECLE 
      CLTWIHLSC ARIKKNNIP EVFLCATCK KKQRSVSPT 
      SPPDKAVKR PRWFKFRKK SGSTMAPKG NNMIPNGHF 
      HKDWQRFVK TWFNQPARK IRRKQNRIK KARAVAPRP 
      AAGALRPIV RCPTVRYHT KVRAGRGFT LQELKAAGV 
      NSNLAKTIG IAVDYRRRN KSVESVLLN AQRLKEYRS 
      KLILFPINN KKKLRPGEA TEEERKVAT QLTSEVLPI 
      RQSAVRTKA RVPTEEEQN FNAFITLRK ARADARFVG 
      IRAKRAKEA ADNPDDVTK AAKPKGKK
    CDS sequence
    • >TC031495 RA
      atggaacag aaaaactct gttacagaa gttgctgga 
      atgtcaaat ggttataga cgtccaaga actagtgcc 
      gatttttat gatttttgt acagtggtt ttgcaatat 
      gccaattac gaaggctgg aaaaaagac gatctacga 
      aacgagact aaccaaagc cctcccttg gacagctca 
      gggagtagt gtcaattct gagtccacc agctcaggg 
      cagaaggat aaacagagt gaagacgat gaggatagc 
      gactctttt aacttagtt acttgtttt tgtggcaaa 
      ccctttgca ggtagacct atgatagaa tgtttggag 
      tgtctaaca tggatacac ctctcctgc gctcggatt 
      aaaaaaaat aatatacca gaagtgttc ctatgtgcc 
      acatgcaaa aagaagcag cgatctgtg tctcccact 
      agtcccccc gacaaagct gtgaaacgt ccgcgttgg 
      tttaagttt cgcaaaaaa tctggatca accatggcg 
      ccgaaggga aataacatg atcccgaat ggtcacttt 
      cacaaggat tggcaaagg tttgttaaa acttggttc 
      aaccaacct gcgcggaaa atacgcagg aagcagaac 
      cgtatcaag aaagctagg gctgtcgct cctagacct 
      gcagcgggg gctttgaga cctatcgtc aggtgcccc 
      acagtcagg tatcacact aaagtgcgt gcagggcgc 
      ggatttacc ctacaggag ttgaaggct gccggagtt 
      aacagcaat ttagctaag accattggc attgctgtt 
      gattacaga aggaggaat aagtccgtt gaatctgtg 
      ctcctaaac gcccaacgt ttgaaggaa tacaggtcc 
      aaacttatc ttgttcccc atcaacaac aagaagaag 
      ttgaggcct ggtgaagcc actgaggag gaaagaaag 
      gtcgccact cagttgaca tctgaagtg ttgccaatc 
      cgccaatca gcggtcaga acaaaagcg agagttcca 
      actgaagaa gaacagaac ttcaacgct tttatcact 
      cttagaaag gcgcgtgct gatgctcgc tttgttggt 
      atccgtgct aagagagcg aaggaagcc gccgataat 
      cctgatgat gtaacaaaa gcggctaag ccaaaaggc 
      aagaagtaa
    mRNA sequence
    • >TC031495 RA
      tacaacctg actgacatc agaattttc caaagttta 
      gtgtagaac gcgaataaa tataaataa aataactct 
      cttcttagt gcctatctt atgtttaac caatcgaaa 
      aatttcaaa acatagtcc tttcccagg taatgacaa 
      ataaggagt tgtcattac cccccgcaa ttatggaac 
      agaaaaact ctgttacag aagttgctg gaatgtcaa 
      atggttata gacgtccaa gaactagtg ccgattttt 
      atgattttt gtacagtgg ttttgcaat atgccaatt 
      acgaaggct ggaaaaaag acgatctac gaaacgaga 
      ctaaccaaa gccctccct tggacagct cagggagta 
      gtgtcaatt ctgagtcca ccagctcag ggcagaagg 
      ataaacaga gtgaagacg atgaggata gcgactctt 
      ttaacttag ttacttgtt tttgtggca aaccctttg 
      caggtagac ctatgatag aatgtttgg agtgtctaa 
      catggatac acctctcct gcgctcgga ttaaaaaaa 
      ataatatac cagaagtgt tcctatgtg ccacatgca 
      aaaagaagc agcgatctg tgtctccca ctagtcccc 
      ccgacaaag ctgtgaaac gtccgcgtt ggtttaagt 
      ttcgcaaaa aatctggat caaccatgg cgccgaagg 
      gaaataaca tgatcccga atggtcact ttcacaagg 
      attggcaaa ggtttgtta aaacttggt tcaaccaac 
      ctgcgcgga aaatacgca ggaagcaga accgtatca 
      agaaagcta gggctgtcg ctcctagac ctgcagcgg 
      gggctttga gacctatcg tcaggtgcc ccacagtca 
      ggtatcaca ctaaagtgc gtgcagggc gcggattta 
      ccctacagg agttgaagg ctgccggag ttaacagca 
      atttagcta agaccattg gcattgctg ttgattaca 
      gaaggagga ataagtccg ttgaatctg tgctcctaa 
      acgcccaac gtttgaagg aatacaggt ccaaactta 
      tcttgttcc ccatcaaca acaagaaga agttgaggc 
      ctggtgaag ccactgagg aggaaagaa aggtcgcca 
      ctcagttga catctgaag tgttgccaa tccgccaat 
      cagcggtca gaacaaaag cgagagttc caactgaag 
      aagaacaga acttcaacg cttttatca ctcttagaa 
      aggcgcgtg ctgatgctc gctttgttg gtatccgtg 
      ctaagagag cgaaggaag ccgccgata atcctgatg 
      atgtaacaa aagcggcta agccaaaag gcaagaagt 
      aaaagagac gtttttgta taattaatt aattctaat 
      aaaaaattt acaaacccg atttgttct gttttattt 
      acaattttt acccatttt cgcctcctg aaataaatt 
      taccttcat cct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY