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Gene information
General information
TC014181 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2854676..2857866
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014181
    iBeetle screen iB_05579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus potentially partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014181 RA
      MVWFNNSGG FENEEITKA LWREVIETF NEPCRRAAE 
      KLLCFYAFP DCYLGNPLP LCHEDCIAV KQLFCYKEW 
      AHIEDRKSN GFVFKSRGH FRLPVCEKL PKYQIVNNT 
      ATCSYAGLV ELKVDEITH DCIKGRGRF YQGNVNVTK 
      DGLACQRWD SQKPHSHNS PPDIFPELK NSENYCRNA 
      GGEEKKPWC FTTNSSVRW QHCDIPRCP NSTSEESED 
      AAIAMDTFL SPSFLIIYS VIGLLGVII LTLLILLCH 
      RIHKHRLGY NPTDTAEVN IDLDKLPSN MAYHRHGVT 
      LNPKLEKLE FPRNDIIYI KDLGQGAFG RVFQAKAPG 
      LVSGEEFTL VAVKMLKDD ASEDMQDDF EKEACLLAE 
      FDHPNIVKL LGVCAIGRP MCLLFEYMG KGDLNEFLR 
      QCSPSNYVV RSVVDGEYG SRDVFKDNQ LNHLELLEV 
      ALQIASGMV YLSDRKFVH RDLATRNCL INENMVVKI 
      ADFGLSQKI YLQDYYKGS ERDAIPVRW MPLESILYN 
      KYTPESDVW AFGVCLWEI FSFALQPYF GMTHEEVVR 
      FLKAGNVLA SPENTPAAI YQVMKDCWA QKPVDRPNF 
      RTIHQTLTN IQSNMLAMP LQST
    CDS sequence
    • >TC014181 RA
      atggtgtgg ttcaataat tcgggaggt tttgagaat 
      gaggagata accaaggcg ctttggagg gaagttatc 
      gagactttt aatgaacct tgtcgccga gcagctgaa 
      aaattgtta tgtttctat gcctttcct gactgctac 
      ttgggcaac cctcttcca ctgtgccac gaagattgc 
      attgcagtg aagcagcta ttctgctac aaggagtgg 
      gcacatatt gaagacagg aagtccaat ggtttcgtt 
      ttcaaatct aggggccac ttcagatta ccagtttgc 
      gaaaaatta cccaaatat caaatcgta aataacaca 
      gcgacatgt tcatacgcc ggcctcgtg gagctaaaa 
      gtggacgaa ataacacac gactgtatt aagggacgc 
      ggtcgtttc tatcagggc aacgtgaac gtgaccaaa 
      gacggccta gcttgccaa agatgggac tcccaaaag 
      ccccactcg cacaactcc cctcctgac attttcccc 
      gagttgaaa aactcggaa aattactgc cgaaacgcc 
      ggcggcgag gaaaagaaa ccgtggtgt ttcacaacg 
      aactcgtcg gtacggtgg cagcattgt gacatcccc 
      aggtgtccc aactccact tctgaggag tcagaagat 
      gcagctatc gcaatggac acttttctc tccccttcc 
      ttcctcatt atttactcc gtgatcggg cttttgggc 
      gtaataatc ctgactcta ctaatttta ctctgccat 
      cgaattcac aaacaccgc ttggggtac aaccccacg 
      gacaccgcc gaggttaac atcgacctg gacaagtta 
      ccgagcaac atggcctac caccggcac ggcgtaacc 
      ctgaaccca aagttagaa aaactcgaa tttccccgc 
      aacgacatt atctacatc aaggacttg ggccagggg 
      gccttcggc cgcgttttc caggctaaa gccccgggg 
      cttgtctcg ggcgaagag ttcacgtta gtggccgtc 
      aaaatgctg aaagacgac gcgtcggaa gacatgcaa 
      gacgatttt gaaaaagaa gcgtgtctt ttggccgaa 
      tttgaccac cccaatatc gtgaaattg ctgggggtt 
      tgcgccatc ggacgcccc atgtgcctg ctattcgag 
      tacatgggc aaaggggac ctcaacgaa tttctgcga 
      cagtgctcc cccagcaat tacgtggtg cgcagcgtc 
      gtggacggc gaatacggg agcagggac gtctttaaa 
      gacaaccaa ttgaaccac ctggagctg ctcgaagta 
      gcgctgcag atagcgtca ggaatggtc tatttatct 
      gaccgaaaa ttcgtacac cgggaccta gccacgcga 
      aattgcttg attaacgag aacatggtc gtaaaaatc 
      gccgatttc ggactttcg caaaaaata tatctccaa 
      gattactac aaagggagt gaaagggac gcgattccg 
      gtgcggtgg atgccgttg gagagcatt ctttacaac 
      aagtacacg ccagagtcg gacgtttgg gcctttggg 
      gtgtgtctt tgggagatt ttctcgttt gcgttgcaa 
      ccatacttc gggatgacg catgaagag gtcgtcagg 
      tttttgaag gcggggaac gtgttggca tctccggag 
      aacacaccg gcagctatt taccaagtt atgaaggac 
      tgttgggcc caaaaaccg gtcgacagg ccgaatttt 
      cgcaccata catcagacg ctcactaat atacaaagc 
      aacatgctc gcgatgccc ttgcagtcg acgtag
    mRNA sequence
    • >TC014181 RA
      gggtcgtag ccgctcctt ttttggcaa cattttgac 
      agtttaatg aaattgttt ataatttaa aagtagaaa 
      tgtgattag tggatcagg agtaccaaa taaagtcaa 
      tgactggtt taatggaaa aggaaaaat atgtacctc 
      cttgtaatt ttattggta gtctttagt ataagtaat 
      ggagaaata caaagttat tgtgggatt tacgggggc 
      aagatatgt gaaaattac gtgggacat tcgaagatg 
      gtgtggttc aataattcg ggaggtttt gagaatgag 
      gagataacc aaggcgctt tggagggaa gttatcgag 
      acttttaat gaaccttgt cgccgagca gctgaaaaa 
      ttgttatgt ttctatgcc tttcctgac tgctacttg 
      ggcaaccct cttccactg tgccacgaa gattgcatt 
      gcagtgaag cagctattc tgctacaag gagtgggca 
      catattgaa gacaggaag tccaatggt ttcgttttc 
      aaatctagg ggccacttc agattacca gtttgcgaa 
      aaattaccc aaatatcaa atcgtaaat aacacagcg 
      acatgttca tacgccggc ctcgtggag ctaaaagtg 
      gacgaaata acacacgac tgtattaag ggacgcggt 
      cgtttctat cagggcaac gtgaacgtg accaaagac 
      ggcctagct tgccaaaga tgggactcc caaaagccc 
      cactcgcac aactcccct cctgacatt ttccccgag 
      ttgaaaaac tcggaaaat tactgccga aacgccggc 
      ggcgaggaa aagaaaccg tggtgtttc acaacgaac 
      tcgtcggta cggtggcag cattgtgac atccccagg 
      tgtcccaac tccacttct gaggagtca gaagatgca 
      gctatcgca atggacact tttctctcc ccttccttc 
      ctcattatt tactccgtg atcgggctt ttgggcgta 
      ataatcctg actctacta attttactc tgccatcga 
      attcacaaa caccgcttg gggtacaac cccacggac 
      accgccgag gttaacatc gacctggac aagttaccg 
      agcaacatg gcctaccac cggcacggc gtaaccctg 
      aacccaaag ttagaaaaa ctcgaattt ccccgcaac 
      gacattatc tacatcaag gacttgggc cagggggcc 
      ttcggccgc gttttccag gctaaagcc ccggggctt 
      gtctcgggc gaagagttc acgttagtg gccgtcaaa 
      atgctgaaa gacgacgcg tcggaagac atgcaagac 
      gattttgaa aaagaagcg tgtcttttg gccgaattt 
      gaccacccc aatatcgtg aaattgctg ggggtttgc 
      gccatcgga cgccccatg tgcctgcta ttcgagtac 
      atgggcaaa ggggacctc aacgaattt ctgcgacag 
      tgctccccc agcaattac gtggtgcgc agcgtcgtg 
      gacggcgaa tacgggagc agggacgtc tttaaagac 
      aaccaattg aaccacctg gagctgctc gaagtagcg 
      ctgcagata gcgtcagga atggtctat ttatctgac 
      cgaaaattc gtacaccgg gacctagcc acgcgaaat 
      tgcttgatt aacgagaac atggtcgta aaaatcgcc 
      gatttcgga ctttcgcaa aaaatatat ctccaagat 
      tactacaaa gggagtgaa agggacgcg attccggtg 
      cggtggatg ccgttggag agcattctt tacaacaag 
      tacacgcca gagtcggac gtttgggcc tttggggtg 
      tgtctttgg gagattttc tcgtttgcg ttgcaacca 
      tacttcggg atgacgcat gaagaggtc gtcaggttt 
      ttgaaggcg gggaacgtg ttggcatct ccggagaac 
      acaccggca gctatttac caagttatg aaggactgt 
      tgggcccaa aaaccggtc gacaggccg aattttcgc 
      accatacat cagacgctc actaatata caaagcaac 
      atgctcgcg atgcccttg cagtcgacg tagttgtat 
      aatggtcac tttatgttt tgtttagga aaaatgtga 
      tatgtatag tttggagac ttgtgttac tgcaccgct 
      taggttatt ttcgtttct aataaaacc cattatgca 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY