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Gene information
General information
TC014172 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2777406..2782940
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014172
    iBeetle screen iB_05578

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014172 RA
      MLPDSKLWF VFSVFSGGE AAHAVKRFT GLYTGPYFD 
      PTTTTNITT QLGTHAYLP CKVKQLGNK SVSWIRRRD 
      AHILTVDRY TFIADDRFQ AFLVEATDT WTLQVKYVQ 
      PRDAGQYEC QVSTEPKMS HFITLNVVV PKIEISGES 
      DIYVKTGST VSLKCVITQ SLEEPAYIF WYHDGERVL 
      KYDRSAIDI RTDRVGTDT TVSTLIIFH ARTEDSGNY 
      TCSPSNLDS ASVLLHVLN GEHPAAMQR GKNSASPIP 
      AWSHLSMGL GLAACTPPR FAIAVALSL AVLVELLAT
    CDS sequence
    • >TC014172 RA
      atgcttcca gattctaag ttatggttt gttttttca 
      gtgttttcc ggcggagaa gcagcccat gcagttaaa 
      agattcaca ggattatac actgggcca tactttgat 
      cccacaacg accaccaac ataacgaca cagttggga 
      acgcacgca tacctccct tgtaaagta aaacagttg 
      ggcaacaaa tctgtgtcg tggatcagg aggcgagac 
      gctcatatt ctcaccgta gataggtat actttcatc 
      gccgacgac cgctttcag gctttcctg gtcgaagcc 
      accgacaca tggacacta caagttaag tacgtgcag 
      ccaagggac gccggacag tacgaatgc caagtcagc 
      accgagccc aaaatgagc cacttcatc accctcaac 
      gtcgtagtc ccgaaaatt gagatatcg ggcgaatct 
      gacatttat gtgaagacc ggaagcacc gtgagcctg 
      aaatgcgtg atcacccag tctttggag gaacccgcc 
      tatatcttc tggtatcac gacggcgag cgcgtcctg 
      aaatatgat cggagtgca attgatata cgcacagat 
      cgtgtggga acagacacc acagtgagc acactcatc 
      attttccac gcccggacg gaagattca ggaaactac 
      acctgcagc ccctccaat ctggactca gcaagcgtc 
      ctcctgcac gttctcaac ggcgagcac cctgcggcc 
      atgcaacga ggcaagaac tcggcttct cccatcccg 
      gcttggtcc catctctcc atggggctc ggactcgcc 
      gcctgcaca ccacccaga ttcgccatc gccgtggcc 
      ctcagcctg gcagtcctg gtcgagctg ctggccaca 
      taa
    mRNA sequence
    • >TC014172 RA
      cgaaaatat aattacggc atcacattt atccggctt 
      gatgcttcc agattctaa gttatggtt tgttttttc 
      agtgttttc cggcggaga agcagccca tgcagttaa 
      aagattcac aggattata cactgggcc atactttga 
      tcccacaac gaccaccaa cataacgac acagttggg 
      aacgcacgc atacctccc ttgtaaagt aaaacagtt 
      gggcaacaa atctgtgtc gtggatcag gaggcgaga 
      cgctcatat tctcaccgt agataggta tactttcat 
      cgccgacga ccgctttca ggctttcct ggtcgaagc 
      caccgacac atggacact acaagttaa gtacgtgca 
      gccaaggga cgccggaca gtacgaatg ccaagtcag 
      caccgagcc caaaatgag ccacttcat caccctcaa 
      cgtcgtagt cccgaaaat tgagatatc gggcgaatc 
      tgacattta tgtgaagac cggaagcac cgtgagcct 
      gaaatgcgt gatcaccca gtctttgga ggaacccgc 
      ctatatctt ctggtatca cgacggcga gcgcgtcct 
      gaaatatga tcggagtgc aattgatat acgcacaga 
      tcgtgtggg aacagacac cacagtgag cacactcat 
      cattttcca cgcccggac ggaagattc aggaaacta 
      cacctgcag cccctccaa tctggactc agcaagcgt 
      cctcctgca cgttctcaa cggcgagca ccctgcggc 
      catgcaacg aggcaagaa ctcggcttc tcccatccc 
      ggcttggtc ccatctctc catggggct cggactcgc 
      cgcctgcac accacccag attcgccat cgccgtggc 
      cctcagcct ggcagtcct ggtcgagct gctggccac 
      ataaactac caacgtccg cgcttaaga tcactggtt 
      ccgttttgt tactgattt tttaattag attttatat 
      aactgactt aaggtaaaa gggagaagc gatgagatt 
      gtactataa caaaatttt gcattttag acgttttca 
      ttacattct caatagtgt ttcgtttta cgaccactg 
      tatacgttt tcaaacaat aaatcaaat aactaatgg 
      caaggctcg atttatagg tgtaggtcg gtggcgtac 
      gtttgaaga aaacgaaaa tttctattt tcgtcattg 
      tgaatttct attatttcc atgtgaaaa ggatttgtg 
      taatttatg taacgtgga aacgaaatt tatcgcctc 
      tatttgtga ggacaatct aattttcga tttgttggt 
      gttaaattg gagttggat ccctccata aatgaattt 
      attgagcaa ctgtgataa gactatgtt tattgcatg 
      ctggacgaa tattttcat cgggaattg atggtgatg 
      ttacttgtt ttattgttt gaaaataaa tcgtttttg 
      tcatgtaac attcacgta cacgttact tcaggaact 
      gtcgtcctc tttcatcta catacccac atacataat 
      ataaatgta atttttaat aaatgaatt aacttcatg 
      tgtgaagtg agactacac tacttactg ttgaacttg 
      cagtgtttt gtttgtaaa taattccag gttattcag 
      tgaaacgga cgcatactg aaatgtgag ttaattaag 
      cctaaaatt aaaggtgtg attaattag ataagttag 
      ttagttata aatgtaaaa tgaatatca ttgcagttg 
      tatttctga tatgatggg tgaaagagc tgatgtata 
      aagtatttt tactgtaaa aattttgat tatctgttt 
      aattgtttt taaaaactc gaatgttcg ttttgcaat 
      gctgtaaat ctttcattt taatgttgt acattattc 
      aaaatctag cgttcataa ttcaaataa aaatgtaaa 
      tgatccgct gttactcaa atctgtgaa attattagc 
      ccttaggac ttaccttta ttttatatc aattttatt 
      gatgtattt taggtaagt acagaatgt aacccaact 
      ttatgttga ataaatcgt tataataaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY