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Gene information
General information
TC014162 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6977230..7057270
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014162
    iBeetle screen iB_05576

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014162 RA
      MFNKKRKCQ KLLIFLSIF YFAQAASST GATSNVVVE 
      EPEFTDTIE NITVAAGRN VKFACSVKN LGTYKVAWM 
      HFEQSAILT VHNHVITRN PRISVTHDK HRTWFLHIS 
      NVQEEDKGR YMCQINTVT AKTQFGYLH VVVPPNIDD 
      SLSSSDVIV REGANETLT CKATGSPQP SVKWKRDDN 
      SKITINKTL TVSEWEGET LELTRISRL DMGAYLCIA 
      SNGVPPTVS KRIKVSVDF PPMLWIPHQ LVGAPLAYS 
      VTLECFTEA HPSSLNYWT REDGQMIHE SKKYHAENT 
      VGTPSYKTH MKLTINNIQ QSDYGTYKC VAKNPRGET 
      DGTIRLYTS SPPTTPPKS TTTETPSRI KDILGNGNL 
      SNSVYGNPS SLTIHHGEK GSKYQSNLN EIDKSEQKS 
      SSDPEGKSI SYGHHVPSG VASSPGNLN CKLSLWILP 
      IILFNALR
    CDS sequence
    • >TC014162 RA
      atgtttaat aaaaaaaga aagtgtcaa aaactttta 
      atttttttg agtattttc tactttgct caagctgcc 
      tcctccacg ggggcaacg tctaatgtg gtcgttgaa 
      gaacctgag tttacagac actatagaa aatataact 
      gtagcagcg ggccgcaac gtaaagttc gcatgctcc 
      gtcaagaat cttggaact tacaaggtg gcgtggatg 
      cattttgag cagtcagcc atcttgact gtgcacaac 
      catgtgatc acccgcaat cctcgtatt agcgtaact 
      cacgataaa cataggact tggttttta cacataagt 
      aatgtacaa gaggaggat aaagggcga tatatgtgc 
      caaatcaat accgttact gcgaaaaca cagttcgga 
      tatctccac gttgttgtg ccgcctaac atagacgat 
      tccctcagt agcagcgat gttatagta agggaaggc 
      gccaatgag acgctaacc tgcaaagct acaggatct 
      ccacaacct agtgtaaag tggaaaagg gacgataac 
      tccaagatc acaataaac aagacactt acagtttcc 
      gagtgggaa ggagaaaca ctggaatta actagaatt 
      tcaagatta gacatggga gcttactta tgtatagcc 
      agcaatggg gtgccccca actgtgagc aaaagaatc 
      aaagttagc gtcgacttt ccgccaatg ttgtggata 
      ccccaccaa ctggtcgga gctcctcta gcttactcc 
      gtgactctg gaatgcttc acggaagct catccgagc 
      tcccttaac tactggacc agagaggat ggccaaatg 
      attcacgaa tcgaagaag tatcacgct gaaaataca 
      gtcggcacg ccgtcttac aagacccat atgaaactc 
      acaatcaac aatatacaa caaagtgat tacgggact 
      tataagtgc gtagcaaag aatccccga ggtgaaacc 
      gatggcaca atcagactc tacacttct tcacctcca 
      acgacgcca ccaaagtca acaactaca gaaacgcca 
      agtcgcatc aaggatata ttgggcaat ggcaatctc 
      agcaattcg gtttatgga aatccttct tcgctcact 
      attcatcat ggtgagaaa ggttcgaaa tatcaatca 
      aatttaaat gaaattgac aaatccgag cagaaatca 
      agttcagac ccagaggga aagagtatc agctatggt 
      caccacgtg ccaagcgga gtagctagt tctccaggt 
      aacctaaac tgcaagctt tcattgtgg atacttccc 
      ataatattg ttcaacgcg ctgcggtga
    mRNA sequence
    • >TC014162 RA
      gtctgaatg tgaaaagca cgcgctcgg aaatttcga 
      tcgaaactc aaaacgaac tgtgttgta aggactgtc 
      cgttatgtt taataaaaa aagaaagtg tcaaaaact 
      tttaatttt tttgagtat tttctactt tgctcaagc 
      tgcctcctc cacgggggc aacgtctaa tgtggtcgt 
      tgaagaacc tgagtttac agacactat agaaaatat 
      aactgtagc agcgggccg caacgtaaa gttcgcatg 
      ctccgtcaa gaatcttgg aacttacaa ggtggcgtg 
      gatgcattt tgagcagtc agccatctt gactgtgca 
      caaccatgt gatcacccg caatcctcg tattagcgt 
      aactcacga taaacatag gacttggtt tttacacat 
      aagtaatgt acaagagga ggataaagg gcgatatat 
      gtgccaaat caataccgt tactgcgaa aacacagtt 
      cggatatct ccacgttgt tgtgccgcc taacataga 
      cgattccct cagtagcag cgatgttat agtaaggga 
      aggcgccaa tgagacgct aacctgcaa agctacagg 
      atctccaca acctagtgt aaagtggaa aagggacga 
      taactccaa gatcacaat aaacaagac acttacagt 
      ttccgagtg ggaaggaga aacactgga attaactag 
      aatttcaag attagacat gggagctta cttatgtat 
      agccagcaa tggggtgcc cccaactgt gagcaaaag 
      aatcaaagt tagcgtcga ctttccgcc aatgttgtg 
      gatacccca ccaactggt cggagctcc tctagctta 
      ctccgtgac tctggaatg cttcacgga agctcatcc 
      gagctccct taactactg gaccagaga ggatggcca 
      aatgattca cgaatcgaa gaagtatca cgctgaaaa 
      tacagtcgg cacgccgtc ttacaagac ccatatgaa 
      actcacaat caacaatat acaacaaag tgattacgg 
      gacttataa gtgcgtagc aaagaatcc ccgaggtga 
      aaccgatgg cacaatcag actctacac ttcttcacc 
      tccaacgac gccaccaaa gtcaacaac tacagaaac 
      gccaagtcg catcaagga tatattggg caatggcaa 
      tctcagcaa ttcggttta tggaaatcc ttcttcgct 
      cactattca tcatggtga gaaaggttc gaaatatca 
      atcaaattt aaatgaaat tgacaaatc cgagcagaa 
      atcaagttc agacccaga gggaaagag tatcagcta 
      tggtcacca cgtgccaag cggagtagc tagttctcc 
      aggtaacct aaactgcaa gctttcatt gtggatact 
      tcccataat attgttcaa cgcgctgcg gtgaatacc 
      tcaaaacac aaattttgt gtatccatt ataattact 
      atttagtag attatagga acagtaagt tcggattat 
      aaaatcaca aggacgctt aaaaccaaa gcataagtg 
      tacaattgt ttatatata ccatgtatc acctaattt 
      aaacaaaga ctgacatcg tttagagaa atataatta 
      atttttaac ataattgac tagttgata attcctgaa 
      ttttcgagg catcaatta ttcagtcga ttacacacg 
      ttacgcaag tggttttga tttgcctca tttccaaaa 
      atatgtgct acgttctga atattaaat cgtttgcat 
      cataaacaa ttcacttgt gtttcattc ttggatcat 
      agtacgttt ttgtttatt gtattaaat tgaagcaag 
      aatttcatt tgctaagtc tctagataa cttctcgtg 
      ccttaaatt ataaaccaa ttgtttcat aaaaaatta 
      acggactct aatttgact tttgctaag ttgttaaat 
      tttcagacg atagattta ctaaaatat atgatgcaa 
      acgaactgt cactgggcg tttataatt ttaagagac 
      ctggaacgg ttttagcag aaacgcaaa ttattctaa 
      caaaaccgt tccatagtt atccaactg ggaaaaatt 
      atttaaaag gttcaccaa tggatattt ggctgggaa 
      tactatagc tcctcaata tttatgtag aagcacaaa 
      atatcaatt tcatgtagc ttttgtagc gtccaaaaa 
      tgttactgt agttcataa ttaatttaa gagtgtaga 
      ctttgtata taatattta ataagccgt ttatttttt 
      aaattctgt tctggttgt acatcaaat gaaaatatt 
      taatcaatt tgtttttta acactgaat gaacgtggt 
      acgaacaca aaaacattt cggttatga ttaagttac 
      ctagacagt gtacatatt tcaaacatt tttgtgacg 
      actaaacga gtctcaaaa gttttactt aaacatttt 
      ctaaatagt tgaataatt ttttttgac gaattttcc 
      aaaatgttc ttaaaattt gttcacatt tcttgttgc 
      gccccaaac tgacggtac ccgttcttc tgaacttta 
      aactgacag ggtattaga aattttagt gatgtactg 
      atattgaac aaataatta ttttcattt gttgtatat 
      gaattttat ttagtaaat cggtgacta aaaaaatgg 
      agtaggcta cactctgta ctaaaatct accttattc 
      ttttagtaa ataaagcgt attgttttg tcaatgtgc 
      caagttaaa tattttaat aataagtac tattttgtt 
      ggaaaaata acctccaat agtacactg ttgtagata 
      aacgatgtt tcgctacaa atataaacg actgtgttt 
      taaaataac ataaattat aaattgaaa ttgtatttg 
      cgtatataa acattgtat cgaaaatgt atataataa 
      agatatata gataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY