Status
Please wait ...
Gene information
General information
TC014160 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6960025..6963462
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014160
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014160 RA
      MIKLYLILF LSTALSADD RFKGCYKTI FSTQTYNVS 
      WTTSEQCIN ECKTRYYRY AMVQNARSC YCGNYLGEE 
      VNNTLSCDQ ICDGFCDNV TDVFATGNL VPGPPEEVK 
      LTNVTDSSA HISWKPPKS YTKINGYVI KATVIHTYA 
      NYIPNSPEW SYLNDTFST KIILLPATK YNVTLCALS 
      PDGCGAPFS RVIETEVRE PENPPESPN IIKRDGDRM 
      EIELSETSN SNGPITSYL VIVFNDDSQ QIFQEDSLK 
      SYYEAKREG INYYITAEL KPENIQRRF IVGDKNYYG 
      KYYNAPLET NVHYSIIIG LVSTLNGKT KRAFSSLKH 
      TDTDGTEEF IEVSGDSPA VIIGLSVAI GLLSFMFIA 
      GIIGFIILK SRVTTRRQR LSENQELTL QGPMIEVEN 
      NGYIPEEEH TKVSYYRDL KQKVRTIPY NQLKVEPTN 
      LLGIGRFGR VNSGSIYEN STLIPVAVY SIQDKKLSA 
      DDKKNMLRD LDVLIKTRK HDNVIALVG TCETSQMVF 
      VVLEYVSMN LKDLLLGSR DSLPGKFSN MTENQALDI 
      AINVAKGMS HLASNKIIH KQLCARNVM VANGFIPKI 
      SGYGLSQFY SHNNLPDYT RWTAMEVFK GQAHNLKSD 
      VWSFGCLLW EICVLGGTP YGNFSNNEI PERIIKGLR 
      LPQMQYFSD ELYQFMLSC WQIDMDERP DFDVLLESL 
      QTFQENSLI PCLSFNLYP NFQYEQFYP DMEIAVRPV 
      F
    CDS sequence
    • >TC014160 RA
      atgatcaag ctctattta atactgttc ttaagtacg 
      gctttaagc gcagatgat agattcaaa ggttgttac 
      aaaacgatt ttctcaaca caaacctac aacgtgtct 
      tggacaaca agcgagcaa tgtatcaac gagtgcaaa 
      acgagatat tacaggtac gccatggtc caaaacgct 
      aggtcatgc tattgtggc aattatctg ggcgaggaa 
      gttaacaac acactgtct tgcgaccaa atttgtgac 
      gggttttgc gacaacgta accgacgtg tttgcgacc 
      ggcaactta gtccccggg cccccagaa gaagtaaaa 
      ctaacgaac gtgaccgac tcaagtgcc cacatttct 
      tggaagccg ccaaagtct tacacgaaa ataaacggt 
      tacgtgata aaagccaca gtgattcac acttacgct 
      aattacatt ccaaatagc ccggaatgg tcgtatttg 
      aacgacacg ttcagcaca aaaattatc cttttaccg 
      gccactaaa tataatgta actttatgc gcgctctcg 
      cccgatggt tgtggggcg cccttttcg cgagtaatc 
      gagactgaa gttcgagaa cccgaaaat ccacccgaa 
      agtccgaac attatcaaa cgagacggg gacagaatg 
      gaaattgag ttatcagag acgtcgaat agcaatggg 
      cctataacg tcctatcta gtcattgtt tttaatgac 
      gacagccaa caaatattc caagaggac tcactaaag 
      tcctattat gaagccaag cgggaaggt ataaactat 
      tacattacg gctgagttg aagccagag aacattcag 
      aggagattt attgttggc gataagaat tattacgga 
      aaatactat aacgcgcct ttggagacc aatgttcac 
      tacagcatt ataataggg cttgtgagc actttaaat 
      ggcaaaacg aaaagagct ttcagtagt ttaaaacat 
      acggatacg gacggcact gaagaattt attgaagtc 
      agtggtgac tcgcccgct gttattatc ggactgtca 
      gttgctatc ggtttgctg agttttatg tttattgcc 
      ggtatcatc gggtttatt attttgaaa agtcgagtc 
      accactaga agacagaga ctgtcggaa aatcaggag 
      ttgaccctt cagggacca atgatcgaa gttgaaaac 
      aacggatac atccctgaa gaagagcac acgaaagtg 
      agctactac cgggatctg aagcagaag gtgaggacg 
      attccttac aatcagcta aaagtcgag cctacaaat 
      ttgctgggc attggccgt tttgggcga gttaacagt 
      ggctcgatt tacgagaac agcacctta ataccagtg 
      gcagtgtac agtattcaa gacaagaaa ttgtcggcg 
      gacgataaa aagaacatg cttcgagat ttagacgtt 
      ctgattaaa acgagaaaa cacgacaat gttattgcc 
      ttagtgggc acttgtgag acgtcacag atggtgttt 
      gttgtccta gagtatgta tcaatgaat ctcaaagat 
      ctactgttg ggcagtaga gactcactg cctgggaaa 
      tttagcaac atgactgag aatcaagct ttagatatt 
      gctataaat gtagcgaaa gggatgtcc cacttagca 
      tctaataag attattcat aagcagctt tgtgcgcgc 
      aatgtcatg gtggcgaac gggttcatc cccaagata 
      agcggctat ggactgtcg cagttttat agtcacaat 
      aacctgcct gactacacc cgctggacg gcgatggaa 
      gtgttcaaa ggccaggcg cataatttg aaaagtgat 
      gtttggtct tttggatgc ttgttgtgg gagatttgt 
      gttttagga gggactcct tatggtaat ttctcaaat 
      aatgaaata cctgaaaga attattaaa ggtctacgt 
      ttaccgcaa atgcaatat ttcagcgat gaactgtat 
      cagtttatg ctgagctgt tggcagatt gacatggac 
      gaaagacct gactttgat gtcttgctc gaatcgctt 
      caaacgttt caagaaaat tcactgatt ccgtgttta 
      agttttaat ttgtatcca aactttcag tatgaacag 
      ttttaccct gatatggaa attgctgtg aggccagta 
      ttctaa
    mRNA sequence
    • >TC014160 RA
      cgcttcttc gaaaaaatt gacagcgat agctgacag 
      gcagaaata acctgtgaa aagcattta gttttgtaa 
      aataaacaa actgataaa aactgtaaa cacacaata 
      cctctgtaa taaaagaaa agacatcgt ttataagca 
      agttgacga aaaattgag tttggggcg attcacgtg 
      ttacacctg cgattagtc actcagggt gtgttcatt 
      tgcattttt acttagttt aaccttaag agaagcatt 
      tccaataca cttatctat tgatcggtg aaaatattg 
      ctgtatatt ccagctggc aagtttctt ttataatat 
      gctgattaa tgacgactt tactaacag atttgtgag 
      ctgtaaaca cgcagaatg atcaagctc tatttaata 
      ctgttctta agtacggct ttaagcgca gatgataga 
      ttcaaaggt tgttacaaa acgattttc tcaacacaa 
      acctacaac gtgtcttgg acaacaagc gagcaatgt 
      atcaacgag tgcaaaacg agatattac aggtacgcc 
      atggtccaa aacgctagg tcatgctat tgtggcaat 
      tatctgggc gaggaagtt aacaacaca ctgtcttgc 
      gaccaaatt tgtgacggg ttttgcgac aacgtaacc 
      gacgtgttt gcgaccggc aacttagtc cccgggccc 
      ccagaagaa gtaaaacta acgaacgtg accgactca 
      agtgcccac atttcttgg aagccgcca aagtcttac 
      acgaaaata aacggttac gtgataaaa gccacagtg 
      attcacact tacgctaat tacattcca aatagcccg 
      gaatggtcg tatttgaac gacacgttc agcacaaaa 
      attatcctt ttaccggcc actaaatat aatgtaact 
      ttatgcgcg ctctcgccc gatggttgt ggggcgccc 
      ttttcgcga gtaatcgag actgaagtt cgagaaccc 
      gaaaatcca cccgaaagt ccgaacatt atcaaacga 
      gacggggac agaatggaa attgagtta tcagagacg 
      tcgaatagc aatgggcct ataacgtcc tatctagtc 
      attgttttt aatgacgac agccaacaa atattccaa 
      gaggactca ctaaagtcc tattatgaa gccaagcgg 
      gaaggtata aactattac attacggct gagttgaag 
      ccagagaac attcagagg agatttatt gttggcgat 
      aagaattat tacggaaaa tactataac gcgcctttg 
      gagaccaat gttcactac agcattata atagggctt 
      gtgagcact ttaaatggc aaaacgaaa agagctttc 
      agtagttta aaacatacg gatacggac ggcactgaa 
      gaatttatt gaagtcagt ggtgactcg cccgctgtt 
      attatcgga ctgtcagtt gctatcggt ttgctgagt 
      tttatgttt attgccggt atcatcggg tttattatt 
      ttgaaaagt cgagtcacc actagaaga cagagactg 
      tcggaaaat caggagttg acccttcag ggaccaatg 
      atcgaagtt gaaaacaac ggatacatc cctgaagaa 
      gagcacacg aaagtgagc tactaccgg gatctgaag 
      cagaaggtg aggacgatt ccttacaat cagctaaaa 
      gtcgagcct acaaatttg ctgggcatt ggccgtttt 
      gggcgagtt aacagtggc tcgatttac gagaacagc 
      accttaata ccagtggca gtgtacagt attcaagac 
      aagaaattg tcggcggac gataaaaag aacatgctt 
      cgagattta gacgttctg attaaaacg agaaaacac 
      gacaatgtt attgcctta gtgggcact tgtgagacg 
      tcacagatg gtgtttgtt gtcctagag tatgtatca 
      atgaatctc aaagatcta ctgttgggc agtagagac 
      tcactgcct gggaaattt agcaacatg actgagaat 
      caagcttta gatattgct ataaatgta gcgaaaggg 
      atgtcccac ttagcatct aataagatt attcataag 
      cagctttgt gcgcgcaat gtcatggtg gcgaacggg 
      ttcatcccc aagataagc ggctatgga ctgtcgcag 
      ttttatagt cacaataac ctgcctgac tacacccgc 
      tggacggcg atggaagtg ttcaaaggc caggcgcat 
      aatttgaaa agtgatgtt tggtctttt ggatgcttg 
      ttgtgggag atttgtgtt ttaggaggg actccttat 
      ggtaatttc tcaaataat gaaatacct gaaagaatt 
      attaaaggt ctacgttta ccgcaaatg caatatttc 
      agcgatgaa ctgtatcag tttatgctg agctgttgg 
      cagattgac atggacgaa agacctgac tttgatgtc 
      ttgctcgaa tcgcttcaa acgtttcaa gaaaattca 
      ctgattccg tgtttaagt tttaatttg tatccaaac 
      tttcagtat gaacagttt taccctgat atggaaatt 
      gctgtgagg ccagtattc taatttgtc ttcggttaa 
      tattggaac ctactaatg catccagca caaatttat 
      gacatgctc tattctaat gtatgaatt tctagaacg 
      ttgttccat ttatgcata tctctattg aaaatgaca 
      ttgttttaa ttgtcttac tggacttcc tgtaataat 
      tttagcatg ccactcagc tgatattat atgtttata 
      atcctcgtg ttgatacca tttttgcgc agctaggtg 
      gaaatccgt cattttaaa agacttaaa gaatctgct 
      aaacactga atattaagc accgtagtt ttaggttcg 
      aggcgtaaa ataggtttc aatattttg tacttactg 
      gttactttt atgtattaa attatacat tccattttt 
      atctcgaat aatttatgt atgatattg tatttttac 
      acgataccg cctattttt ataataata aattttttt 
      acacatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY