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Gene information
General information
TC014156 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6797510..6801606
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014156
    iBeetle screen iB_05573

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: antenna can be shortened, malformed or missing
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014156 RA
      MTQTDAPNS DIIQKSIGV LGKWHIAIY VMIFLVKFP 
      VAWHQLGIV FLAPPVEFS CIDNKTDKC SADCNEHIF 
      DRSIFTETI IIQWDLVCA RTYLATMTQ TDAPNSDII 
      QKSIGVLGK WHIAICVMI FLVKFPVAW HQLGIVFLA 
      PPVEFSCID NKTDKCSAD CNEHIFDRS IFTETIITQ 
      WDLVCARTY LASLAQTIT MLGILFGNM LFGYLSDRF 
      GRKAPLTGA VVLQVVSGI CTAFVPYFS LFLVMRFLT 
      ALATGGTMV ISFVLVMEL VGLEWRTTM GILYQIPFN 
      LGHMIMPLF SFLLRDWKY FQIAISTPA VILISYYWI 
      LPESPRWLL TVGKKEKAI KLLEKAARC NKLPTEQIK 
      QDVYNYLKM NETSEENQA GNIFDLIKT PIMRMYTFC 
      VGFNWFVCG LCFYGSAQY IGQMGGNIF LNVALSGVI 
      QIPSTFFSI WAVKAWGRK RTLIFSNIL AGISCLLIA 
      LVPEEPAWI RITLSCVDM FALIVSFPT VYIYSGELF 
      PTVVRNIGV GTSSMCARF GSMAAPFIA GLITVQTWL 
      PPVVFGVVP LIGALMCIK LPETLDCKL PDTIKEAEL 
      FVTNSEK
    CDS sequence
    • >TC014156 RA
      atgacacag acagatgct ccaaactcg gacataatc 
      cagaaatca atcggtgtt ctgggaaag tggcacata 
      gcaatatat gtcatgatt ttccttgta aaatttccg 
      gtggcttgg caccagctc ggcattgtg tttctagca 
      ccccctgta gaattctct tgcattgat aacaaaact 
      gataaatgt tccgctgat tgtaacgag cacattttt 
      gacagatca atttttacc gaaactata atcatccaa 
      tgggacctc gtgtgtgcc aggacgtat ctagcaaca 
      atgacgcag acagatgct ccaaactcg gacataatc 
      cagaaatca atcggagtt ctgggaaag tggcacata 
      gcaatatgt gtaatgatt ttccttgta aaatttccg 
      gtggcttgg caccagctc ggcattgtg tttctagca 
      ccccctgta gaattctct tgcattgat aacaaaact 
      gataaatgt tccgctgat tgtaacgag cacattttt 
      gacagatca atttttacc gaaactata atcactcaa 
      tgggacctc gtgtgtgcc aggacgtat ctagcaagt 
      ctggcccag actataacc atgcttggt attcttttc 
      gggaacatg cttttcggt tacctttct gataggttt 
      ggacggaaa gctccactg actggggcc gtggtcttg 
      caagttgtt agtgggatt tgtacggcg tttgttccc 
      tatttttca ctctttctc gttatgcga tttttaact 
      gctttggcc acgggaggc accatggtc attagtttt 
      gtgctagtg atggagtta gtaggacta gaatggcgg 
      accacgatg ggaatcctc taccaaatc cccttcaat 
      ctcgggcat atgataatg cccttattt tcgtttctt 
      ctaagggac tggaaatat ttccaaatt gcgatatca 
      acccctgct gttatttta atttcgtac tactggatc 
      ctcccagag tcgcctcgt tggttgcta accgttggc 
      aagaaggag aaagcgatc aagcttttg gaaaaagca 
      gctcgttgc aacaaactc ccaacggaa caaatcaaa 
      caagacgtt tacaactac ttgaaaatg aacgaaacg 
      agtgaagaa aaccaagct ggcaacatt ttcgattta 
      ataaaaacg ccaataatg cgaatgtac acattttgc 
      gttggcttc aactggttc gtgtgcggc ttatgcttc 
      tacggaagc gcacaatac atcggccaa atgggaggg 
      aacattttc cttaacgtt gccctttct ggtgttatt 
      caaatcccg agcactttc ttctcaatt tgggctgtg 
      aaagcgtgg ggacgcaaa cgcacctta atttttagc 
      aacatttta gcagggatc tcctgtctt ttgatagct 
      ctggtcccc gaagaacca gcttggatc aggataact 
      ttgagctgt gtagacatg tttgcatta attgtgtct 
      ttcccaacg gtgtatatt tattcgggt gaactcttt 
      ccaactgtt gttcggaat ataggcgtg gggacatcg 
      tcaatgtgc gcaaggttt ggctccatg gctgcaccg 
      tttattgca gggcttatt acagtgcag acgtggttg 
      ccaccggtt gtttttggg gttgtgccc ctcattggg 
      gctctgatg tgtattaaa ttacccgag acgctcgac 
      tgcaaattg cctgacacg attaaagaa gcggaactc 
      tttgtaacg aattcggag aaataa
    mRNA sequence
    • >TC014156 RA
      tgataacaa aactgataa atgttccgc tgattgtaa 
      cgagcacat ttttgacaa gtgtgatta ttttttgta 
      attttaaaa tcaattttt caaacacag atcaatttt 
      taccgaaac tataatcac ccaatggga cctcgtgtg 
      tgccaggac gtatctagc aagtctggc ccagactat 
      aaccatgct taaaactac ccgagacac ttaactgca 
      cactgccgg atacggtcg aggaagcgg agcagtttg 
      ttaaaaaag cgctagcta ataacgaca cagaaatga 
      gataaatta tgaagtaca aaatttcat tgttgaaat 
      ctattttta acactttaa attaacaaa tttggagtc 
      tttcaataa tgtgtattt tttttaata aatagcgtt 
      acttgtggt tttggccat aattcaatc ctcccggcg 
      catccacca aaatttgac aaaaataaa ttggctact 
      caatgacac agacagatg ctccaaact cggacataa 
      tccagaaat caatcggtg ttctgggaa agtggcaca 
      tagcaatat atgtcatga ttttccttg taaaatttc 
      cggtggctt ggcaccagc tcggcattg tgtttctag 
      caccccctg tagaattct cttgcattg ataacaaaa 
      ctgataaat gttccgctg attgtaacg agcacattt 
      ttgacagat caattttta ccgaaacta taatcatcc 
      aatgggacc tcgtgtgtg ccaggacgt atctagcaa 
      caatgacgc agacagatg ctccaaact cggacataa 
      tccagaaat caatcggag ttctgggaa agtggcaca 
      tagcaatat gtgtaatga ttttccttg taaaatttc 
      cggtggctt ggcaccagc tcggcattg tgtttctag 
      caccccctg tagaattct cttgcattg ataacaaaa 
      ctgataaat gttccgctg attgtaacg agcacattt 
      ttgacagat caattttta ccgaaacta taatcactc 
      aatgggacc tcgtgtgtg ccaggacgt atctagcaa 
      gtctggccc agactataa ccatgcttg gtattcttt 
      tcgggaaca tgcttttcg gttaccttt ctgataggt 
      ttggacgga aagctccac tgactgggg ccgtggtct 
      tgcaagttg ttagtggga tttgtacgg cgtttgttc 
      cctattttt cactctttc tcgttatgc gatttttaa 
      ctgctttgg ccacgggag gcaccatgg tcattagtt 
      ttgtgctag tgatggagt tagtaggac tagaatggc 
      ggaccacga tgggaatcc tctaccaaa tccccttca 
      atctcgggc atatgataa tgcccttat tttcgtttc 
      ttctaaggg actggaaat atttccaaa ttgcgatat 
      caacccctg ctgttattt taatttcgt actactgga 
      tcctcccag agtcgcctc gttggttgc taaccgttg 
      gcaagaagg agaaagcga tcaagcttt tggaaaaag 
      cagctcgtt gcaacaaac tcccaacgg aacaaatca 
      aacaagacg tttacaact acttgaaaa tgaacgaaa 
      cgagtgaag aaaaccaag ctggcaaca ttttcgatt 
      taataaaaa cgccaataa tgcgaatgt acacatttt 
      gcgttggct tcaactggt tcgtgtgcg gcttatgct 
      tctacggaa gcgcacaat acatcggcc aaatgggag 
      ggaacattt tccttaacg ttgcccttt ctggtgtta 
      ttcaaatcc cgagcactt tcttctcaa tttgggctg 
      tgaaagcgt ggggacgca aacgcacct taattttta 
      gcaacattt tagcaggga tctcctgtc ttttgatag 
      ctctggtcc ccgaagaac cagcttgga tcaggataa 
      ctttgagct gtgtagaca tgtttgcat taattgtgt 
      ctttcccaa cggtgtata tttattcgg gtgaactct 
      ttccaactg ttgttcgga atataggcg tggggacat 
      cgtcaatgt gcgcaaggt ttggctcca tggctgcac 
      cgtttattg cagggctta ttacagtgc agacgtggt 
      tgccaccgg ttgtttttg gggttgtgc ccctcattg 
      gggctctga tgtgtatta aattacccg agacgctcg 
      actgcaaat tgcctgaca cgattaaag aagcggaac 
      tctttgtaa cgaattcgg agaaataaa aaaatgaac 
      tgttcaata aacaaatta tccaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY