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Gene information
General information
TC014155 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6793080..6796653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014155
    iBeetle screen iB_05572

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014155 RA
      MSEKDATNS DIIQKSIGV FGRWHVAVC FIIFLVKFP 
VAWHQLSIV FLAPPMKFS CIDNDTDKC SSNCSEHIF 
DRTIFAETI ITQWDLVCG RTHLASLAQ TVTMFGILS 
GNMLFGYLS DRFGRRGPL TGAVILQVV SGTCTAFAP 
YFPLFLFMR FLAALSTGG TMVISFVLV MELVGLEWR 
TTLGILYQI PFNLGHLLL PLFAYFLRD WRYFQITIS 
LPAVILISY YWILPESPR WLLTVGKKD KAIKLLEKA 
AHYNKLPTE HIKEDVYNY MQKNEINEE KQAGNILDL 
VKTPIMRMY TFCVGFNWF VCGLCFFGS AQYIGQLGG 
NIFINVALS AVIQIPSTL FSIWAVKAW GRKRTLISS 
NILAGISCL LIAIVPGKL AWVRTTLSC VDMFALAVS 
FPTVYIYSG ELFPTVVRN IGVGTSSMC ARFGSMAAP 
FIAGLVIVQ AWLPPVIFG VVPLIGALL CIKLPETLN 
CTLPDTVEE AEQFVKKAL ANNDTEMR
    CDS sequence
    • >TC014155 RA
      atgtcagaa aaagacgcc acaaactcg gacataatc 
caaaaatca attggggtt tttggacga tggcacgtg 
gcagtttgt ttcattatt tttctcgtc aaattccca 
gtggcttgg caccaactc agcatcgta tttctagca 
cctccgatg aaattctct tgcatagat aacgacaca 
gacaaatgc tccagtaac tgtagcgaa cacattttt 
gacagaaca atctttgcg gagacaatc atcactcaa 
tgggaccta gtctgtggc agaacgcat ttagcaagt 
ctggcccaa actgtgacg atgtttggg attctgtcc 
gggaacatg cttttcgga tatctttct gataggttt 
ggacggaga ggcccattg actggggcg gtgatcttg 
caggttgtt tctgggact tgcactgcg tttgctccc 
tatttcccg ctcttcctg tttatgcga tttttggcc 
gctttgtcc acgggcggc acaatggtc attagtttt 
gtgctagta atggaacta gtagggcta gagtggcga 
acaacgttg ggcatcctc taccaaatt cccttcaat 
ctcggccat ttgttattg cccctcttt gcttatttt 
ctccgcgac tggagatac ttccaaatt acgatatca 
ctacctgcc gttatttta atttcctac tactggatc 
ctcccagag tcgcctcgg tggttgcta acagtcggc 
aaaaaggac aaagcgatc aagcttttg gaaaaagcc 
gctcattat aacaaactc ccaacggaa cacatcaaa 
gaggacgtt tacaactac atgcaaaaa aacgaaatc 
aatgaagag aaacaagcc ggcaacatt ctagactta 
gtaaaaaca ccaataatg cgaatgtac acattttgc 
gtcggcttc aactggttc gtatgcggg ctatgtttc 
ttcggaagt gcccagtac atcggacag ttgggcgga 
aacattttc atcaacgtt gccctatct gctgttatc 
caaatcccg agcacctta ttctcaatc tgggctgtg 
aaagcgtgg gggcgtaaa cgcacctta atctccagc 
aacatttta gcaggaatt tcctgtctt ttgatcgcc 
atagtccca gggaaactg gcttgggtt cgcacaacg 
ttgagttgt gtggacatg ttcgcgtta gctgtgtct 
ttcccaaca gtttacatt tattcaggg gaacttttc 
ccgactgtt gtacgaaat ataggcgtg gggacttcg 
tcaatgtgt gctaggttt ggctcaatg gctgctccg 
ttcattgca ggacttgtt atagtccag gcgtggtta 
cccccggtt atttttggg gttgttccg ctcattggg 
gcactgctt tgtatcaaa ctaccagag acacttaac 
tgcacactg ccggatacg gtcgaggaa gcggagcag 
tttgttaaa aaagcgcta gctaataac gacacagaa 
atgcgataa
    mRNA sequence
    • >TC014155 RA
      caccgattg tatctgtgt cgagtccac ctcgaagtt 
gtgtggcat accataacg gtgctcgcg aatttcaag 
tgcattcgg tttttgtgc tacaccaac agcaacctt 
caatgaatt tcaattcac tttgtgtgt aatatttgg 
ggaaaacaa tgtcagaaa aagacgcca caaactcgg 
acataatcc aaaaatcaa ttggggttt ttggacgat 
ggcacgtgg cagtttgtt tcattattt ttctcgtca 
aattcccag tggcttggc accaactca gcatcgtat 
ttctagcac ctccgatga aattctctt gcatagata 
acgacacag acaaatgct ccagtaact gtagcgaac 
acatttttg acagaacaa tctttgcgg agacaatca 
tcactcaat gggacctag tctgtggca gaacgcatt 
tagcaagtc tggcccaaa ctgtgacga tgtttggga 
ttctgtccg ggaacatgc ttttcggat atctttctg 
ataggtttg gacggagag gcccattga ctggggcgg 
tgatcttgc aggttgttt ctgggactt gcactgcgt 
ttgctccct atttcccgc tcttcctgt ttatgcgat 
ttttggccg ctttgtcca cgggcggca caatggtca 
ttagttttg tgctagtaa tggaactag tagggctag 
agtggcgaa caacgttgg gcatcctct accaaattc 
ccttcaatc tcggccatt tgttattgc ccctctttg 
cttattttc tccgcgact ggagatact tccaaatta 
cgatatcac tacctgccg ttattttaa tttcctact 
actggatcc tcccagagt cgcctcggt ggttgctaa 
cagtcggca aaaaggaca aagcgatca agcttttgg 
aaaaagccg ctcattata acaaactcc caacggaac 
acatcaaag aggacgttt acaactaca tgcaaaaaa 
acgaaatca atgaagaga aacaagccg gcaacattc 
tagacttag taaaaacac caataatgc gaatgtaca 
cattttgcg tcggcttca actggttcg tatgcgggc 
tatgtttct tcggaagtg cccagtaca tcggacagt 
tgggcggaa acattttca tcaacgttg ccctatctg 
ctgttatcc aaatcccga gcaccttat tctcaatct 
gggctgtga aagcgtggg ggcgtaaac gcaccttaa 
tctccagca acattttag caggaattt cctgtcttt 
tgatcgcca tagtcccag ggaaactgg cttgggttc 
gcacaacgt tgagttgtg tggacatgt tcgcgttag 
ctgtgtctt tcccaacag tttacattt attcagggg 
aacttttcc cgactgttg tacgaaata taggcgtgg 
ggacttcgt caatgtgtg ctaggtttg gctcaatgg 
ctgctccgt tcattgcag gacttgtta tagtccagg 
cgtggttac ccccggtta tttttgggg ttgttccgc 
tcattgggg cactgcttt gtatcaaac taccagaga 
cacttaact gcacactgc cggatacgg tcgaggaag 
cggagcagt ttgttaaaa aagcgctag ctaataacg 
acacagaaa tgcgataag ttatgaagt acaaaattt 
cattgttga aattaattt ttaatactt taagttagc 
gaatttgga gtctttcaa taatgtgta tttttttaa 
taaatagcg ttaattgtg gttttggcc ataatttaa 
tcctcccgg cgcatccac caaaatttg acaaaaata 
aattgacta ctgtaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY