Status
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Gene information
General information
TC014155 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6793080..6796653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014155
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05572

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014155 RA
      MSEKDATNS DIIQKSIGV FGRWHVAVC FIIFLVKFP 
      VAWHQLSIV FLAPPMKFS CIDNDTDKC SSNCSEHIF 
      DRTIFAETI ITQWDLVCG RTHLASLAQ TVTMFGILS 
      GNMLFGYLS DRFGRRGPL TGAVILQVV SGTCTAFAP 
      YFPLFLFMR FLAALSTGG TMVISFVLV MELVGLEWR 
      TTLGILYQI PFNLGHLLL PLFAYFLRD WRYFQITIS 
      LPAVILISY YWILPESPR WLLTVGKKD KAIKLLEKA 
      AHYNKLPTE HIKEDVYNY MQKNEINEE KQAGNILDL 
      VKTPIMRMY TFCVGFNWF VCGLCFFGS AQYIGQLGG 
      NIFINVALS AVIQIPSTL FSIWAVKAW GRKRTLISS 
      NILAGISCL LIAIVPGKL AWVRTTLSC VDMFALAVS 
      FPTVYIYSG ELFPTVVRN IGVGTSSMC ARFGSMAAP 
      FIAGLVIVQ AWLPPVIFG VVPLIGALL CIKLPETLN 
      CTLPDTVEE AEQFVKKAL ANNDTEMR
    CDS sequence
    • >TC014155 RA
      atgtcagaa aaagacgcc acaaactcg gacataatc 
      caaaaatca attggggtt tttggacga tggcacgtg 
      gcagtttgt ttcattatt tttctcgtc aaattccca 
      gtggcttgg caccaactc agcatcgta tttctagca 
      cctccgatg aaattctct tgcatagat aacgacaca 
      gacaaatgc tccagtaac tgtagcgaa cacattttt 
      gacagaaca atctttgcg gagacaatc atcactcaa 
      tgggaccta gtctgtggc agaacgcat ttagcaagt 
      ctggcccaa actgtgacg atgtttggg attctgtcc 
      gggaacatg cttttcgga tatctttct gataggttt 
      ggacggaga ggcccattg actggggcg gtgatcttg 
      caggttgtt tctgggact tgcactgcg tttgctccc 
      tatttcccg ctcttcctg tttatgcga tttttggcc 
      gctttgtcc acgggcggc acaatggtc attagtttt 
      gtgctagta atggaacta gtagggcta gagtggcga 
      acaacgttg ggcatcctc taccaaatt cccttcaat 
      ctcggccat ttgttattg cccctcttt gcttatttt 
      ctccgcgac tggagatac ttccaaatt acgatatca 
      ctacctgcc gttatttta atttcctac tactggatc 
      ctcccagag tcgcctcgg tggttgcta acagtcggc 
      aaaaaggac aaagcgatc aagcttttg gaaaaagcc 
      gctcattat aacaaactc ccaacggaa cacatcaaa 
      gaggacgtt tacaactac atgcaaaaa aacgaaatc 
      aatgaagag aaacaagcc ggcaacatt ctagactta 
      gtaaaaaca ccaataatg cgaatgtac acattttgc 
      gtcggcttc aactggttc gtatgcggg ctatgtttc 
      ttcggaagt gcccagtac atcggacag ttgggcgga 
      aacattttc atcaacgtt gccctatct gctgttatc 
      caaatcccg agcacctta ttctcaatc tgggctgtg 
      aaagcgtgg gggcgtaaa cgcacctta atctccagc 
      aacatttta gcaggaatt tcctgtctt ttgatcgcc 
      atagtccca gggaaactg gcttgggtt cgcacaacg 
      ttgagttgt gtggacatg ttcgcgtta gctgtgtct 
      ttcccaaca gtttacatt tattcaggg gaacttttc 
      ccgactgtt gtacgaaat ataggcgtg gggacttcg 
      tcaatgtgt gctaggttt ggctcaatg gctgctccg 
      ttcattgca ggacttgtt atagtccag gcgtggtta 
      cccccggtt atttttggg gttgttccg ctcattggg 
      gcactgctt tgtatcaaa ctaccagag acacttaac 
      tgcacactg ccggatacg gtcgaggaa gcggagcag 
      tttgttaaa aaagcgcta gctaataac gacacagaa 
      atgcgataa
    mRNA sequence
    • >TC014155 RA
      caccgattg tatctgtgt cgagtccac ctcgaagtt 
      gtgtggcat accataacg gtgctcgcg aatttcaag 
      tgcattcgg tttttgtgc tacaccaac agcaacctt 
      caatgaatt tcaattcac tttgtgtgt aatatttgg 
      ggaaaacaa tgtcagaaa aagacgcca caaactcgg 
      acataatcc aaaaatcaa ttggggttt ttggacgat 
      ggcacgtgg cagtttgtt tcattattt ttctcgtca 
      aattcccag tggcttggc accaactca gcatcgtat 
      ttctagcac ctccgatga aattctctt gcatagata 
      acgacacag acaaatgct ccagtaact gtagcgaac 
      acatttttg acagaacaa tctttgcgg agacaatca 
      tcactcaat gggacctag tctgtggca gaacgcatt 
      tagcaagtc tggcccaaa ctgtgacga tgtttggga 
      ttctgtccg ggaacatgc ttttcggat atctttctg 
      ataggtttg gacggagag gcccattga ctggggcgg 
      tgatcttgc aggttgttt ctgggactt gcactgcgt 
      ttgctccct atttcccgc tcttcctgt ttatgcgat 
      ttttggccg ctttgtcca cgggcggca caatggtca 
      ttagttttg tgctagtaa tggaactag tagggctag 
      agtggcgaa caacgttgg gcatcctct accaaattc 
      ccttcaatc tcggccatt tgttattgc ccctctttg 
      cttattttc tccgcgact ggagatact tccaaatta 
      cgatatcac tacctgccg ttattttaa tttcctact 
      actggatcc tcccagagt cgcctcggt ggttgctaa 
      cagtcggca aaaaggaca aagcgatca agcttttgg 
      aaaaagccg ctcattata acaaactcc caacggaac 
      acatcaaag aggacgttt acaactaca tgcaaaaaa 
      acgaaatca atgaagaga aacaagccg gcaacattc 
      tagacttag taaaaacac caataatgc gaatgtaca 
      cattttgcg tcggcttca actggttcg tatgcgggc 
      tatgtttct tcggaagtg cccagtaca tcggacagt 
      tgggcggaa acattttca tcaacgttg ccctatctg 
      ctgttatcc aaatcccga gcaccttat tctcaatct 
      gggctgtga aagcgtggg ggcgtaaac gcaccttaa 
      tctccagca acattttag caggaattt cctgtcttt 
      tgatcgcca tagtcccag ggaaactgg cttgggttc 
      gcacaacgt tgagttgtg tggacatgt tcgcgttag 
      ctgtgtctt tcccaacag tttacattt attcagggg 
      aacttttcc cgactgttg tacgaaata taggcgtgg 
      ggacttcgt caatgtgtg ctaggtttg gctcaatgg 
      ctgctccgt tcattgcag gacttgtta tagtccagg 
      cgtggttac ccccggtta tttttgggg ttgttccgc 
      tcattgggg cactgcttt gtatcaaac taccagaga 
      cacttaact gcacactgc cggatacgg tcgaggaag 
      cggagcagt ttgttaaaa aagcgctag ctaataacg 
      acacagaaa tgcgataag ttatgaagt acaaaattt 
      cattgttga aattaattt ttaatactt taagttagc 
      gaatttgga gtctttcaa taatgtgta tttttttaa 
      taaatagcg ttaattgtg gttttggcc ataatttaa 
      tcctcccgg cgcatccac caaaatttg acaaaaata 
      aattgacta ctgtaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY