Status
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Gene information
General information
TC014154 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6787700..6791266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014154
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05571

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014154 RA
        MKHRNVSNF LLLLLCGAV FSDDSLVVS TTTKPEKEV 
        TAETETKND TEINPKIYS MHIDSNVTN RFATTLVTS 
        KVRNLKKTA AETTFSVVL PENAFISAF EMEIEGKIY 
        KAHVKEKQE AKQIYQEAV SRGQSAAHV ELNARDSKT 
        FTVSVNIEP ESKTTFRLT YEELLKRQL GQYELVINI 
        HPGQLVDDL DVRVHINES RPLTFVKTP SLRTGNEIS 
        KNADKLDPS AKIEIMNST SALVEFSPD KGKQKEFGQ 
        LLGSEKESG LAGQFIVQY DVERDPQGG EVLVRDGYF 
        VHFFSPSEL KPLPKHIVF VLDHSGSMS GRKIDQLIE 
        AMQNILTDL KETDLFNIV RFGDLAMVW DVSQNQFTQ 
        LPNFNEYGN LEPHLREIN LPRAVNGTE ENIEAAKKI 
        IEDKSRLGM TNMMYGLEV GLFLIKRTQ EETPDKYQP 
        MIVFLTDGH PNAGMSGRD EITNTVTSL NSGKKKASI 
        FSLSFGDFA DKRFLRKIS SKNSGFSRH IYESSDASL 
        QLQDFYRAI SAPLLSNVN FKYVDDVKD VTVTHYPIL 
        FNGSELVVA GRINDNPHV LNHVEAWCP IGPIKFNAT 
        INTEAVSSL ERLWAYLTV KQMLKQKET AENAANLTK 
        KALDLSLKY SFVTPITSL VVVKPNQTE AVDSEAAKS 
        SPGRYDGAR SAVAPSFSA PVPAYYPGM SSGISGRSF 
        ISSDYSGRA ALYFPPPDR FFPPPPVNF RTTRRPYFL 
        KPSSTSLPP PQNAIVPQW LEIMRTNNG NITTLQGIY 
        KLGQSGAVT TRPQCPKTP TGTVGECVI IFDCPEIFK 
        DLPDLNTYL QYFCPLNEF AGVCCPPKI PAPLP
      CDS sequence
      • >TC014154 RA
        atgaagcac cgaaacgta tcaaatttc ttgctgctg 
        cttctgtgt ggtgcagtt ttttcagac gatagttta 
        gtggtgtcc accaccacc aaacctgaa aaagaagtc 
        accgcagaa acagaaacc aaaaatgac accgaaatc 
        aatccaaaa atatactcc atgcacatt gattcaaac 
        gtaacgaat cgctttgcc acaacacta gtgactagc 
        aaagtgcga aatttaaag aaaacagcg gcggaaacc 
        accttctcg gttgtcttg cccgaaaat gccttcatc 
        tcggcgttt gaaatggaa atcgaggga aaaatatac 
        aaagcgcat gttaaagaa aaacaggag gctaaacaa 
        atctatcaa gaggccgtg tcaagagga caaagtgcg 
        gctcatgtc gaactaaat gcccgagat tcgaaaaca 
        tttacagtt tcggtgaat atcgaacca gaaagcaag 
        acaacattt agattgact tatgaggag ctgcttaag 
        cgccagctt gggcaatat gagctcgtt attaacata 
        catcctggc caacttgtt gacgatctg gacgtcaga 
        gttcatatc aacgaaagc aggcccttg acattcgtc 
        aaaacgccc tctttgagg acaggaaac gagattagt 
        aaaaacgcc gacaagttg gacccttcg gccaaaata 
        gaaatcatg aattcgact tccgctctc gttgaattt 
        agccccgac aaaggcaag cagaaagag tttggtcaa 
        cttttaggg agtgagaag gagagtgga ctagctggg 
        cagtttatc gtccaatat gacgttgaa cgagatcct 
        caaggcgga gaggttttg gtccgcgac ggctacttc 
        gtccacttc ttctctcca agtgagctg aagccgttg 
        cccaaacac atcgtgttc gtcctggac cacagtggc 
        agcatgagc ggcaggaaa atcgaccag ttgatcgaa 
        gcaatgcag aacatttta acagacttg aaagagacc 
        gatttgttc aacattgtt cgcttcggg gacttggca 
        atggtctgg gatgtgtcc caaaaccag ttcacccag 
        cttccaaat tttaacgaa tatggcaac cttgaacca 
        cacttgaga gagataaat ttgccacgg gccgtcaac 
        ggcacggaa gaaaatatt gaagcggca aaaaaaatc 
        atcgaggat aagtccaga ctagggatg actaacatg 
        atgtacggc ctggaagtt ggcttattc ctgatcaaa 
        cgaacacaa gaagaaacg cctgacaag taccaaccc 
        atgattgtg ttcttgacc gacggtcat cccaacgct 
        gggatgtcg gggagagac gagatcaca aacaccgtg 
        acgagtctt aacagtggg aaaaaaaag gcgtcgatt 
        ttctcactt tcgtttggg gactttgcc gataaacgc 
        ttcttgaga aaaatatct tcgaagaat tcgggcttt 
        tcgagacat atttacgaa tcttcagac gcctcactg 
        caattgcaa gacttctac cgcgcgatt tctgcaccc 
        ctgctaagc aatgttaat ttcaaatac gttgatgat 
        gtcaaagac gttaccgta actcattat ccgattctt 
        ttcaacggc tcggaactg gttgtagca ggaaggata 
        aatgataac ccccatgtg ttaaatcat gtggaggct 
        tggtgtccc ataggaccc attaaattt aacgccacc 
        atcaacact gaagctgtt agttccttg gaacgcctc 
        tgggcctat ttaactgtt aagcaaatg ttgaagcag 
        aaggaaacg gcggaaaat gcggctaat ttaactaaa 
        aaagcactt gatttgtcc ctaaaatac tcgtttgtt 
        actccgatc acgtctttg gtagttgtt aaacctaat 
        cagactgaa gctgtcgat agtgaggcg gctaagtcc 
        agtccagga cggtacgat ggggctcgt tcggccgtt 
        gcaccttca ttttcggcg cctgttcct gcatattat 
        ccaggaatg agtagtgga atttcagga cgatcattc 
        attagttca gattactca ggaagagca gctttgtat 
        ttcccccca ccagatcgc tttttccca ccgccacca 
        gttaacttc aggaccact cgaagacct tactttctg 
        aaaccatcg tccacgagc cttccaccc cctcaaaat 
        gcaatcgta cctcagtgg ctcgaaatt atgagaaca 
        aataatgga aacataact acgctgcaa ggtatctac 
        aaattgggt cagagcggg gctgttaca acacgtccc 
        caatgtccc aaaacacca acaggaact gtcggagaa 
        tgcgtcatt atttttgac tgtcctgaa atatttaag 
        gacttgccg gatctcaac acatatctg caatacttt 
        tgtccttta aatgagttt gctggagtt tgctgtccg 
        ccaaaaata ccagcgcca ttgccgtaa
      mRNA sequence
      • >TC014154 RA
        gctgttgaa acaaattat gtatctcaa agtttgaag 
        aagctcacg aaatgaagc accgaaacg tatcaaatt 
        tcttgctgc tgcttctgt gtggtgcag ttttttcag 
        acgatagtt tagtggtgt ccaccacca ccaaacctg 
        aaaaagaag tcaccgcag aaacagaaa ccaaaaatg 
        acaccgaaa tcaatccaa aaatatact ccatgcaca 
        ttgattcaa acgtaacga atcgctttg ccacaacac 
        tagtgacta gcaaagtgc gaaatttaa agaaaacag 
        cggcggaaa ccaccttct cggttgtct tgcccgaaa 
        atgccttca tctcggcgt ttgaaatgg aaatcgagg 
        gaaaaatat acaaagcgc atgttaaag aaaaacagg 
        aggctaaac aaatctatc aagaggccg tgtcaagag 
        gacaaagtg cggctcatg tcgaactaa atgcccgag 
        attcgaaaa catttacag tttcggtga atatcgaac 
        cagaaagca agacaacat ttagattga cttatgagg 
        agctgctta agcgccagc ttgggcaat atgagctcg 
        ttattaaca tacatcctg gccaacttg ttgacgatc 
        tggacgtca gagttcata tcaacgaaa gcaggccct 
        tgacattcg tcaaaacgc cctctttga ggacaggaa 
        acgagatta gtaaaaacg ccgacaagt tggaccctt 
        cggccaaaa tagaaatca tgaattcga cttccgctc 
        tcgttgaat ttagccccg acaaaggca agcagaaag 
        agtttggtc aacttttag ggagtgaga aggagagtg 
        gactagctg ggcagttta tcgtccaat atgacgttg 
        aacgagatc ctcaaggcg gagaggttt tggtccgcg 
        acggctact tcgtccact tcttctctc caagtgagc 
        tgaagccgt tgcccaaac acatcgtgt tcgtcctgg 
        accacagtg gcagcatga gcggcagga aaatcgacc 
        agttgatcg aagcaatgc agaacattt taacagact 
        tgaaagaga ccgatttgt tcaacattg ttcgcttcg 
        gggacttgg caatggtct gggatgtgt cccaaaacc 
        agttcaccc agcttccaa attttaacg aatatggca 
        accttgaac cacacttga gagagataa atttgccac 
        gggccgtca acggcacgg aagaaaata ttgaagcgg 
        caaaaaaaa tcatcgagg ataagtcca gactaggga 
        tgactaaca tgatgtacg gcctggaag ttggcttat 
        tcctgatca aacgaacac aagaagaaa cgcctgaca 
        agtaccaac ccatgattg tgttcttga ccgacggtc 
        atcccaacg ctgggatgt cggggagag acgagatca 
        caaacaccg tgacgagtc ttaacagtg ggaaaaaaa 
        aggcgtcga ttttctcac tttcgtttg gggactttg 
        ccgataaac gcttcttga gaaaaatat cttcgaaga 
        attcgggct tttcgagac atatttacg aatcttcag 
        acgcctcac tgcaattgc aagacttct accgcgcga 
        tttctgcac ccctgctaa gcaatgtta atttcaaat 
        acgttgatg atgtcaaag acgttaccg taactcatt 
        atccgattc ttttcaacg gctcggaac tggttgtag 
        caggaagga taaatgata acccccatg tgttaaatc 
        atgtggagg cttggtgtc ccataggac ccattaaat 
        ttaacgcca ccatcaaca ctgaagctg ttagttcct 
        tggaacgcc tctgggcct atttaactg ttaagcaaa 
        tgttgaagc agaaggaaa cggcggaaa atgcggcta 
        atttaacta aaaaagcac ttgatttgt ccctaaaat 
        actcgtttg ttactccga tcacgtctt tggtagttg 
        ttaaaccta atcagactg aagctgtcg atagtgagg 
        cggctaagt ccagtccag gacggtacg atggggctc 
        gttcggccg ttgcacctt cattttcgg cgcctgttc 
        ctgcatatt atccaggaa tgagtagtg gaatttcag 
        gacgatcat tcattagtt cagattact caggaagag 
        cagctttgt atttccccc caccagatc gctttttcc 
        caccgccac cagttaact tcaggacca ctcgaagac 
        cttactttc tgaaaccat cgtccacga gccttccac 
        cccctcaaa atgcaatcg tacctcagt ggctcgaaa 
        ttatgagaa caaataatg gaaacataa ctacgctgc 
        aaggtatct acaaattgg gtcagagcg gggctgtta 
        caacacgtc cccaatgtc ccaaaacac caacaggaa 
        ctgtcggag aatgcgtca ttatttttg actgtcctg 
        aaatattta aggacttgc cggatctca acacatatc 
        tgcaatact tttgtcctt taaatgagt ttgctggag 
        tttgctgtc cgccaaaaa taccagcgc cattgccgt 
        aacacctga attttacta tacatgtgt accttttaa 
        caaacatac caaaaataa attttatac tctact
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY