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Gene information
General information
TC014149 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6675183..6749385
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014149
    iBeetle screen iB_05569

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    head orientation irregular - Number of animals showing the phenotype: 6

    wings shortened - Number of animals showing the phenotype: 6
    legs orientation irregular - Number of animals showing the phenotype: 6
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014149 RA
      MNNLLNGKD SRWLQLEVC REFQRNKCS RPDTECKFA 
      HPPANVEVQ NGRVTACYD SIKGRCNRE KPPCKYFHP 
      PQHLKDQLL INGRNHLAL KNALMQQMG LTPGQPLVP 
      GQVQAVATN PYLTGMPQV GSTYSPYFA PGPIMPTIM 
      GPDPTGVGS PLGVVPQTV VAQQKMPRS DRLEVCREY 
      QRGACKRAE SECRFAHPA DSVTANEDG TVTVCMDAV 
      KGRCNRDPC RYFHPPLHL QAQIKAAQS RASAMDMKS 
      VGSFYYDSF AFPAMMPYK RPAADKSGV PVYQPNATT 
      YQQLMQLQQ PFVPVSCEY SVSPAPNPT TSCASPAAP 
      PQSQQEITA ATTTVSQPA KENEVSSPV SSTASSAPP 
      TTLPSLPLP QPTAIPDPA ALAKEVAQQ NYAKAVKLA 
      AVSQSLAAG QLNQLNPLN YTGVALNKQ ALTVPPPAL 
      TRYPTGALP FGLGTAGLN LGFANPYSM AQQNLLNIS 
      RPPPAIINP YSIIRTSYP TANSTPLLG SGLLGTQYP 
      VTVSAPPMT TVTASANIA AAAAQNNNN VVIQPYKKL 
      KTT
    • >TC014149 RB
      MNNLLNGKD SRWLQLEVC REFQRNKCS RPDTECKFA 
      HPPANVEVQ NGRVTACYD SIKGRCNRE KPPCKYFHP 
      PQHLKDQLL INGRNHLAL KNALMQQMG LTPGQPLVP 
      GQVQAVATN PYLTGMPQV GSTYSPYFA PGPIMPTIM 
      GPDPTGVGS PLGVVPQTV VAQQKMPRS DRLEMDMKS 
      VGSFYYDSF AFPAMMPYK RPAADKSGV PVYQPNATT 
      YQQLMQLQQ PFVPVSCEY SVSPAPNPT TSCASPAAP 
      PQSQQEITA ATTTVSQPA KENEVSSPV SSTASSAPP 
      TTLPSLPLP QPTAIPDPA ALAKEVAQQ NYAKAVKLA 
      AVSQSLAAG QLNQLNPLN YTGVALNKQ ALTVPPPAL 
      TRYPTGALP FGLGTAGLN LGFANPYSM AQQNLLNIS 
      RPPPAIINP YSIIRTSYP TANSTPLLG SGLLGTQYP 
      VTVSAPPMT TVTASANIA AAAAQNNNN VVIQPYKKL 
      KTT
    • >TC014149 RC
      MNNLLNGKD SRWLQLEVC REFQRNKCS RPDTECKFA 
      HPPANVEVQ NGRVTACYD SIKGRCNRE KPPCKYFHP 
      PQHLKDQLL INGRNHLAL KNALMQQMG LTPGQPLVP 
      GQVQAVATN PYLTGMPQV GSTYSPYFA PGPIMPTIM 
      GPDPTGVGS PLGVVPQTV VAQQKMPRS DRLEVCREY 
      QRGACKRAE SECRFAHPA DSVTANEDG TVTVCMDAV 
      KGRCNRDPC RYFHPPLHL QAQIKAAQS RASAPAAVS 
      PLLAPPQAT AALEIGKKR PREPSTADT DLLLMDMKS 
      VGSFYYDSF AFPAMMPYK RPAADKSGV PVYQPNATT 
      YQQLMQLQQ PFVPVSCEY SVSPAPNPT TSCASPAAP 
      PQSQQEITA ATTTVSQPA KENEVSSPV SSTASSAPP 
      TTLPSLPLP QPTAIPDPA ALAKEVAQQ NYAKAVKLA 
      AVSQSLAAG QLNQLNPLN YTGVALNKQ ALTVPPPAL 
      TRYPTGALP FGLGTAGLN LGFANPYSM AQQNLLNIS 
      RPPPAIINP YSIIRTSYP TANSTPLLG SGLLGTQYP 
      VTVSAPPMT TVTASANIA AAAAQNNNN VVIQPYKKL 
      KTT
    CDS sequence
    • >TC014149 RA
      atgaataat ttacttaat ggcaaggac tcccgctgg 
      cttcagctt gaagtctgc agagaattc caaaggaac 
      aaatgctcg agaccggac accgagtgt aaatttgca 
      catccacca gccaacgtc gaggtccaa aacggccgc 
      gtgacggcg tgctacgac agtattaag ggtcgctgc 
      aacagagaa aaaccccca tgcaagtac ttccaccct 
      ccacaacac ctcaaggac caacttctt ataaatggt 
      aggaatcac ttggcgtta aaaaatgca cttatgcaa 
      caaatgggt ctaacgccc ggacagcct ttggttcct 
      ggtcaagtt caggcagtg gccacaaac ccctacctg 
      actggaatg ccacaggtt ggcagtaca tatagtcca 
      tattttgct ccgggtcct ataatgcct acgattatg 
      gggccggat cctaccgga gtcgggtcg cctttgggt 
      gtagttcca caaacagtt gtagcacaa caaaaaatg 
      cctcgttcc gatagactt gaggtgtgc cgcgaatac 
      cagcgcggg gcgtgcaag cgagcagag tcagaatgt 
      cgctttgcg catcccgct gactctgta actgccaac 
      gaggacggc actgtcact gtctgcatg gatgcagtc 
      aagggccgc tgcaaccgc gacccctgc cgctatttc 
      cacccccct ctgcacctc caagcccaa atcaaggcc 
      gcacagtcg cgggctagc gcgatggat atgaaatct 
      gttgggtca ttttattat gatagtttt gccttcccg 
      gccatgatg ccgtacaag aggccagcg gcggacaaa 
      tccggtgtt cctgtgtac caaccaaac gcaaccact 
      taccaacaa ctgatgcag ttacagcag ccattcgtg 
      cccgtgtca tgtgagtat tcggtctcc ccagctccg 
      aatcccacc acctcctgc gcttctcct gctgctcct 
      cctcaatca cagcaggaa atcacagca gcaacaaca 
      acggtgtcg caaccagcc aaggagaac gaggtttcc 
      agccccgtc tcctcgact gcttcgtct gcaccccca 
      accacccta ccatctctg cctctgcct caacctact 
      gccatacca gatcccgca gctcttgct aaggaggtc 
      gctcaacag aattacgcc aaagccgtt aaactagcc 
      gccgtaagt cagtcgtta gctgcaggc cagctgaac 
      caactcaac cccctgaac tacacgggg gtcgccttg 
      aacaagcaa gcgctgact gtgccccct cctgccttg 
      acgcgttac ccaaccggc gccctgccg ttcggcttg 
      ggcacggcg ggcctgaac ctgggtttt gcgaaccct 
      tattcgatg gcccagcaa aatttgttg aacatttcg 
      cgcccgccg ccggcgatt attaacccc tattcgatt 
      atccgaact tcttatccc actgcgaac tcaacccct 
      ctcttgggc agtggcctc ttgggcacg caataccct 
      gtcactgtt tctgcaccg cctatgacg acagtcacc 
      gcaagcgct aacatagcc gccgccgcg gcgcaaaat 
      aacaataac gtggtcatt cagccttat aagaaactc 
      aagactaca taa
    • >TC014149 RB
      atgaataat ttacttaat ggcaaggac tcccgctgg 
      cttcagctt gaagtctgc agagaattc caaaggaac 
      aaatgctcg agaccggac accgagtgt aaatttgca 
      catccacca gccaacgtc gaggtccaa aacggccgc 
      gtgacggcg tgctacgac agtattaag ggtcgctgc 
      aacagagaa aaaccccca tgcaagtac ttccaccct 
      ccacaacac ctcaaggac caacttctt ataaatggt 
      aggaatcac ttggcgtta aaaaatgca cttatgcaa 
      caaatgggt ctaacgccc ggacagcct ttggttcct 
      ggtcaagtt caggcagtg gccacaaac ccctacctg 
      actggaatg ccacaggtt ggcagtaca tatagtcca 
      tattttgct ccgggtcct ataatgcct acgattatg 
      gggccggat cctaccgga gtcgggtcg cctttgggt 
      gtagttcca caaacagtt gtagcacaa caaaaaatg 
      cctcgttcc gatagactt gagatggat atgaaatct 
      gttgggtca ttttattat gatagtttt gccttcccg 
      gccatgatg ccgtacaag aggccagcg gcggacaaa 
      tccggtgtt cctgtgtac caaccaaac gcaaccact 
      taccaacaa ctgatgcag ttacagcag ccattcgtg 
      cccgtgtca tgtgagtat tcggtctcc ccagctccg 
      aatcccacc acctcctgc gcttctcct gctgctcct 
      cctcaatca cagcaggaa atcacagca gcaacaaca 
      acggtgtcg caaccagcc aaggagaac gaggtttcc 
      agccccgtc tcctcgact gcttcgtct gcaccccca 
      accacccta ccatctctg cctctgcct caacctact 
      gccatacca gatcccgca gctcttgct aaggaggtc 
      gctcaacag aattacgcc aaagccgtt aaactagcc 
      gccgtaagt cagtcgtta gctgcaggc cagctgaac 
      caactcaac cccctgaac tacacgggg gtcgccttg 
      aacaagcaa gcgctgact gtgccccct cctgccttg 
      acgcgttac ccaaccggc gccctgccg ttcggcttg 
      ggcacggcg ggcctgaac ctgggtttt gcgaaccct 
      tattcgatg gcccagcaa aatttgttg aacatttcg 
      cgcccgccg ccggcgatt attaacccc tattcgatt 
      atccgaact tcttatccc actgcgaac tcaacccct 
      ctcttgggc agtggcctc ttgggcacg caataccct 
      gtcactgtt tctgcaccg cctatgacg acagtcacc 
      gcaagcgct aacatagcc gccgccgcg gcgcaaaat 
      aacaataac gtggtcatt cagccttat aagaaactc 
      aagactaca taa
    • >TC014149 RC
      atgaataat ttacttaat ggcaaggac tcccgctgg 
      cttcagctt gaagtctgc agagaattc caaaggaac 
      aaatgctcg agaccggac accgagtgt aaatttgca 
      catccacca gccaacgtc gaggtccaa aacggccgc 
      gtgacggcg tgctacgac agtattaag ggtcgctgc 
      aacagagaa aaaccccca tgcaagtac ttccaccct 
      ccacaacac ctcaaggac caacttctt ataaatggt 
      aggaatcac ttggcgtta aaaaatgca cttatgcaa 
      caaatgggt ctaacgccc ggacagcct ttggttcct 
      ggtcaagtt caggcagtg gccacaaac ccctacctg 
      actggaatg ccacaggtt ggcagtaca tatagtcca 
      tattttgct ccgggtcct ataatgcct acgattatg 
      gggccggat cctaccgga gtcgggtcg cctttgggt 
      gtagttcca caaacagtt gtagcacaa caaaaaatg 
      cctcgttcc gatagactt gaggtgtgc cgcgaatac 
      cagcgcggg gcgtgcaag cgagcagag tcagaatgt 
      cgctttgcg catcccgct gactctgta actgccaac 
      gaggacggc actgtcact gtctgcatg gatgcagtc 
      aagggccgc tgcaaccgc gacccctgc cgctatttc 
      cacccccct ctgcacctc caagcccaa atcaaggcc 
      gcacagtcg cgggctagc gcgcccgct gccgtctcg 
      cccttgctg gcgcccccc caggcaact gccgccctc 
      gaaatcggc aagaagagg ccccgtgag cctagcacc 
      gccgacaca gatctatta ctgatggat atgaaatct 
      gttgggtca ttttattat gatagtttt gccttcccg 
      gccatgatg ccgtacaag aggccagcg gcggacaaa 
      tccggtgtt cctgtgtac caaccaaac gcaaccact 
      taccaacaa ctgatgcag ttacagcag ccattcgtg 
      cccgtgtca tgtgagtat tcggtctcc ccagctccg 
      aatcccacc acctcctgc gcttctcct gctgctcct 
      cctcaatca cagcaggaa atcacagca gcaacaaca 
      acggtgtcg caaccagcc aaggagaac gaggtttcc 
      agccccgtc tcctcgact gcttcgtct gcaccccca 
      accacccta ccatctctg cctctgcct caacctact 
      gccatacca gatcccgca gctcttgct aaggaggtc 
      gctcaacag aattacgcc aaagccgtt aaactagcc 
      gccgtaagt cagtcgtta gctgcaggc cagctgaac 
      caactcaac cccctgaac tacacgggg gtcgccttg 
      aacaagcaa gcgctgact gtgccccct cctgccttg 
      acgcgttac ccaaccggc gccctgccg ttcggcttg 
      ggcacggcg ggcctgaac ctgggtttt gcgaaccct 
      tattcgatg gcccagcaa aatttgttg aacatttcg 
      cgcccgccg ccggcgatt attaacccc tattcgatt 
      atccgaact tcttatccc actgcgaac tcaacccct 
      ctcttgggc agtggcctc ttgggcacg caataccct 
      gtcactgtt tctgcaccg cctatgacg acagtcacc 
      gcaagcgct aacatagcc gccgccgcg gcgcaaaat 
      aacaataac gtggtcatt cagccttat aagaaactc 
      aagactaca taa
    mRNA sequence
    • >TC014149 RA
      tcacggcgg tccgacgtt cgcttgctt agtacactt 
      tagtttaaa attttattt gtttatgtg tcctgctaa 
      ccgtgtgct gtgtgaaat aactgtcgt cgtgccgtt 
      cccgaatcc ttgtgaagc caccggaat ccttaacgc 
      tttcaaaga ccgccaccg aagaagtta tctgaagac 
      accggactt gcaatatgc gctagaacc gacaatcgc 
      ttcgctttg cctacccgc ttctctgca aattattac 
      tccgttcag aagcttttc tatacccgc cgacccatc 
      agtaacaac gtcaataca gaaaacaca gaacaaaag 
      agtacaaga acgcagtga cccaagatt atttttata 
      tcactctct aacagtttt aagtaacgt tcgttaacg 
      actcgttgt gttgttctt aaaacaatt tttcgaaag 
      gatggacaa taattccac ctgacttga ttttgtgaa 
      ccataacta tatttggag tgaatcgtt gcgcttcgt 
      ccagtgttt ctcgcgtcc aatttgtac gaattattg 
      acaaaaagg ccttcgcgc aatggcaat ggttaacat 
      gaataattt acttaatgg caaggactc ccgctggct 
      tcagcttga agtctgcag agaattcca aaggaacaa 
      atgctcgag accggacac cgagtgtaa atttgcaca 
      tccaccagc caacgtcga ggtccaaaa cggccgcgt 
      gacggcgtg ctacgacag tattaaggg tcgctgcaa 
      cagagaaaa acccccatg caagtactt ccaccctcc 
      acaacacct caaggacca acttcttat aaatggtag 
      gaatcactt ggcgttaaa aaatgcact tatgcaaca 
      aatgggtct aacgcccgg acagccttt ggttcctgg 
      tcaagttca ggcagtggc cacaaaccc ctacctgac 
      tggaatgcc acaggttgg cagtacata tagtccata 
      ttttgctcc gggtcctat aatgcctac gattatggg 
      gccggatcc taccggagt cgggtcgcc tttgggtgt 
      agttccaca aacagttgt agcacaaca aaaaatgcc 
      tcgttccga tagacttga ggtgtgccg cgaatacca 
      gcgcggggc gtgcaagcg agcagagtc agaatgtcg 
      ctttgcgca tcccgctga ctctgtaac tgccaacga 
      ggacggcac tgtcactgt ctgcatgga tgcagtcaa 
      gggccgctg caaccgcga cccctgccg ctatttcca 
      cccccctct gcacctcca agcccaaat caaggccgc 
      acagtcgcg ggctagcgc gatggatat gaaatctgt 
      tgggtcatt ttattatga tagttttgc cttcccggc 
      catgatgcc gtacaagag gccagcggc ggacaaatc 
      cggtgttcc tgtgtacca accaaacgc aaccactta 
      ccaacaact gatgcagtt acagcagcc attcgtgcc 
      cgtgtcatg tgagtattc ggtctcccc agctccgaa 
      tcccaccac ctcctgcgc ttctcctgc tgctcctcc 
      tcaatcaca gcaggaaat cacagcagc aacaacaac 
      ggtgtcgca accagccaa ggagaacga ggtttccag 
      ccccgtctc ctcgactgc ttcgtctgc acccccaac 
      caccctacc atctctgcc tctgcctca acctactgc 
      cataccaga tcccgcagc tcttgctaa ggaggtcgc 
      tcaacagaa ttacgccaa agccgttaa actagccgc 
      cgtaagtca gtcgttagc tgcaggcca gctgaacca 
      actcaaccc cctgaacta cacgggggt cgccttgaa 
      caagcaagc gctgactgt gccccctcc tgccttgac 
      gcgttaccc aaccggcgc cctgccgtt cggcttggg 
      cacggcggg cctgaacct gggttttgc gaaccctta 
      ttcgatggc ccagcaaaa tttgttgaa catttcgcg 
      cccgccgcc ggcgattat taaccccta ttcgattat 
      ccgaacttc ttatcccac tgcgaactc aacccctct 
      cttgggcag tggcctctt gggcacgca ataccctgt 
      cactgtttc tgcaccgcc tatgacgac agtcaccgc 
      aagcgctaa catagccgc cgccgcggc gcaaaataa 
      caataacgt ggtcattca gccttataa gaaactcaa 
      gactacata atttttaac cagttgctc gtcctagtt 
      ttcagtgtt aattttgtg gaatatttt ggctgtgga 
      tattgccat gtaaaattg aacgatgtt aaaaacatt 
      tgcatttta ttgtttgtc gcagtgata tcttaattt 
      acgttatgt gtatattta attgtgtta tctaaattg 
      tggttttaa ttgaataaa taagtgtag ttata
    • >TC014149 RB
      tcacggcgg tccgacgtt cgcttgctt agtacactt 
      tagtttaaa attttattt gtttatgtg tcctgctaa 
      ccgtgtgct gtgtgaaat aactgtcgt cgtgccgtt 
      cccgaatcc ttgtgaagc caccggaat ccttaacgc 
      tttcaaaga ccgccaccg aagaagtta tctgaagac 
      accggactt gcaatatgc gctagaacc gacaatcgc 
      ttcgctttg cctacccgc ttctctgca aattattac 
      tccgttcag aagcttttc tatacccgc cgacccatc 
      agtaacaac gtcaataca gaaaacaca gaacaaaag 
      agtacaaga acgcagtga cccaagatt atttttata 
      tcactctct aacagtttt aagtaacgt tcgttaacg 
      actcgttgt gttgttctt aaaacaatt tttcgaaag 
      gatggacaa taattccac ctgacttga ttttgtgaa 
      ccataacta tatttggag tgaatcgtt gcgcttcgt 
      ccagtgttt ctcgcgtcc aatttgtac gaattattg 
      acaaaaagg ccttcgcgc aatggcaat ggttaacat 
      gaataattt acttaatgg caaggactc ccgctggct 
      tcagcttga agtctgcag agaattcca aaggaacaa 
      atgctcgag accggacac cgagtgtaa atttgcaca 
      tccaccagc caacgtcga ggtccaaaa cggccgcgt 
      gacggcgtg ctacgacag tattaaggg tcgctgcaa 
      cagagaaaa acccccatg caagtactt ccaccctcc 
      acaacacct caaggacca acttcttat aaatggtag 
      gaatcactt ggcgttaaa aaatgcact tatgcaaca 
      aatgggtct aacgcccgg acagccttt ggttcctgg 
      tcaagttca ggcagtggc cacaaaccc ctacctgac 
      tggaatgcc acaggttgg cagtacata tagtccata 
      ttttgctcc gggtcctat aatgcctac gattatggg 
      gccggatcc taccggagt cgggtcgcc tttgggtgt 
      agttccaca aacagttgt agcacaaca aaaaatgcc 
      tcgttccga tagacttga gatggatat gaaatctgt 
      tgggtcatt ttattatga tagttttgc cttcccggc 
      catgatgcc gtacaagag gccagcggc ggacaaatc 
      cggtgttcc tgtgtacca accaaacgc aaccactta 
      ccaacaact gatgcagtt acagcagcc attcgtgcc 
      cgtgtcatg tgagtattc ggtctcccc agctccgaa 
      tcccaccac ctcctgcgc ttctcctgc tgctcctcc 
      tcaatcaca gcaggaaat cacagcagc aacaacaac 
      ggtgtcgca accagccaa ggagaacga ggtttccag 
      ccccgtctc ctcgactgc ttcgtctgc acccccaac 
      caccctacc atctctgcc tctgcctca acctactgc 
      cataccaga tcccgcagc tcttgctaa ggaggtcgc 
      tcaacagaa ttacgccaa agccgttaa actagccgc 
      cgtaagtca gtcgttagc tgcaggcca gctgaacca 
      actcaaccc cctgaacta cacgggggt cgccttgaa 
      caagcaagc gctgactgt gccccctcc tgccttgac 
      gcgttaccc aaccggcgc cctgccgtt cggcttggg 
      cacggcggg cctgaacct gggttttgc gaaccctta 
      ttcgatggc ccagcaaaa tttgttgaa catttcgcg 
      cccgccgcc ggcgattat taaccccta ttcgattat 
      ccgaacttc ttatcccac tgcgaactc aacccctct 
      cttgggcag tggcctctt gggcacgca ataccctgt 
      cactgtttc tgcaccgcc tatgacgac agtcaccgc 
      aagcgctaa catagccgc cgccgcggc gcaaaataa 
      caataacgt ggtcattca gccttataa gaaactcaa 
      gactacata atttttaac cagttgctc gtcctagtt 
      ttcagtgtt aattttgtg gaatatttt ggctgtgga 
      tattgccat gtaaaattg aacgatgtt aaaaacatt 
      tgcatttta ttgtttgtc gcagtgata tcttaattt 
      acgttatgt gtatattta attgtgtta tctaaattg 
      tggttttaa ttgaataaa taagtgtag ttata
    • >TC014149 RC
      accagtctg tgtgtcagt ttcgagaga cgcacatat 
      acactaaac actctgtat actgttcac aacgtataa 
      aaattacaa ttaatgtat tttaaataa aattacagt 
      ctccaattt gctatctag cgtgctgtt ctatacaac 
      gacacaaat ttgcaaggt gtctgaatt gctttttac 
      agtgaacca aaaatattt ttccaagtg cgtccgaag 
      aacgtctag gacaccgcc gggagtttg aaatttcgt 
      aaacatact tggagtaca ttattttgc tgtggaaat 
      tttttgttc agccaatac cgtaacgat agctttctg 
      cctggtttc ttaattttt ggtaaaaca ccggattaa 
      atagttctg gaatgcgtg cttaatttt tgtttggtg 
      gttagtgat tttgctgga gccggtgct gctgcaaaa 
      accgtttcg tctggattt cttataccg ccaccgaag 
      aagttatct gaagacacc ggacttgca atatgcgct 
      agaaccgac aatcgcttc gctttgcct acccgcttc 
      tctgcaaat tattactcc gttcagaag cttttctat 
      acccgccga cccatcagt aacaacgtc aatacagaa 
      aacacagaa caaaagagt acaagaacg cagtgaccc 
      aagattatt tttatatca ctctctaac agttttaag 
      taacgttcg ttaacgact cgttgtgtt gttcttaaa 
      acaattttt cgaaaggat ggacaataa ttccacctg 
      acttgattt tgtgaacca taactatat ttggagtga 
      atcgttgcg cttcgtcca gtgtttctc gcgtccaat 
      ttgtacgaa ttattgaca aaaaggcct tcgcgcaat 
      ggcaatggt taacatgaa taatttact taatggcaa 
      ggactcccg ctggcttca gcttgaagt ctgcagaga 
      attccaaag gaacaaatg ctcgagacc ggacaccga 
      gtgtaaatt tgcacatcc accagccaa cgtcgaggt 
      ccaaaacgg ccgcgtgac ggcgtgcta cgacagtat 
      taagggtcg ctgcaacag agaaaaacc cccatgcaa 
      gtacttcca ccctccaca acacctcaa ggaccaact 
      tcttataaa tggtaggaa tcacttggc gttaaaaaa 
      tgcacttat gcaacaaat gggtctaac gcccggaca 
      gcctttggt tcctggtca agttcaggc agtggccac 
      aaaccccta cctgactgg aatgccaca ggttggcag 
      tacatatag tccatattt tgctccggg tcctataat 
      gcctacgat tatggggcc ggatcctac cggagtcgg 
      gtcgccttt gggtgtagt tccacaaac agttgtagc 
      acaacaaaa aatgcctcg ttccgatag acttgaggt 
      gtgccgcga ataccagcg cggggcgtg caagcgagc 
      agagtcaga atgtcgctt tgcgcatcc cgctgactc 
      tgtaactgc caacgagga cggcactgt cactgtctg 
      catggatgc agtcaaggg ccgctgcaa ccgcgaccc 
      ctgccgcta tttccaccc ccctctgca cctccaagc 
      ccaaatcaa ggccgcaca gtcgcgggc tagcgcgcc 
      cgctgccgt ctcgccctt gctggcgcc cccccaggc 
      aactgccgc cctcgaaat cggcaagaa gaggccccg 
      tgagcctag caccgccga cacagatct attactgat 
      ggatatgaa atctgttgg gtcatttta ttatgatag 
      ttttgcctt cccggccat gatgccgta caagaggcc 
      agcggcgga caaatccgg tgttcctgt gtaccaacc 
      aaacgcaac cacttacca acaactgat gcagttaca 
      gcagccatt cgtgcccgt gtcatgtga gtattcggt 
      ctccccagc tccgaatcc caccacctc ctgcgcttc 
      tcctgctgc tcctcctca atcacagca ggaaatcac 
      agcagcaac aacaacggt gtcgcaacc agccaagga 
      gaacgaggt ttccagccc cgtctcctc gactgcttc 
      gtctgcacc cccaaccac cctaccatc tctgcctct 
      gcctcaacc tactgccat accagatcc cgcagctct 
      tgctaagga ggtcgctca acagaatta cgccaaagc 
      cgttaaact agccgccgt aagtcagtc gttagctgc 
      aggccagct gaaccaact caaccccct gaactacac 
      gggggtcgc cttgaacaa gcaagcgct gactgtgcc 
      ccctcctgc cttgacgcg ttacccaac cggcgccct 
      gccgttcgg cttgggcac ggcgggcct gaacctggg 
      ttttgcgaa cccttattc gatggccca gcaaaattt 
      gttgaacat ttcgcgccc gccgccggc gattattaa 
      cccctattc gattatccg aacttctta tcccactgc 
      gaactcaac ccctctctt gggcagtgg cctcttggg 
      cacgcaata ccctgtcac tgtttctgc accgcctat 
      gacgacagt caccgcaag cgctaacat agccgccgc 
      cgcggcgca aaataacaa taacgtggt cattcagcc 
      ttataagaa actcaagac tacataatt tttaaccag 
      ttgctcgtc ctagttttc agtgttaat tttgtggaa 
      tattttggc tgtggatat tgccatgta aaattgaac 
      gatgttaaa aacatttgc attttattg tttgtcgca 
      gtgatatct taatttacg ttatgtgta tatttaatt 
      gtgttatct aaattgtgg ttttaattg aataaataa 
      gtgtagtta ta
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