| General information | |||
| TC014149 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 6675183..6749385 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014149 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05569 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC014149 RA
MNNLLNGKD SRWLQLEVC REFQRNKCS RPDTECKFA HPPANVEVQ NGRVTACYD SIKGRCNRE KPPCKYFHP PQHLKDQLL INGRNHLAL KNALMQQMG LTPGQPLVP GQVQAVATN PYLTGMPQV GSTYSPYFA PGPIMPTIM GPDPTGVGS PLGVVPQTV VAQQKMPRS DRLEVCREY QRGACKRAE SECRFAHPA DSVTANEDG TVTVCMDAV KGRCNRDPC RYFHPPLHL QAQIKAAQS RASAMDMKS VGSFYYDSF AFPAMMPYK RPAADKSGV PVYQPNATT YQQLMQLQQ PFVPVSCEY SVSPAPNPT TSCASPAAP PQSQQEITA ATTTVSQPA KENEVSSPV SSTASSAPP TTLPSLPLP QPTAIPDPA ALAKEVAQQ NYAKAVKLA AVSQSLAAG QLNQLNPLN YTGVALNKQ ALTVPPPAL TRYPTGALP FGLGTAGLN LGFANPYSM AQQNLLNIS RPPPAIINP YSIIRTSYP TANSTPLLG SGLLGTQYP VTVSAPPMT TVTASANIA AAAAQNNNN VVIQPYKKL KTT
- >TC014149 RB
MNNLLNGKD SRWLQLEVC REFQRNKCS RPDTECKFA HPPANVEVQ NGRVTACYD SIKGRCNRE KPPCKYFHP PQHLKDQLL INGRNHLAL KNALMQQMG LTPGQPLVP GQVQAVATN PYLTGMPQV GSTYSPYFA PGPIMPTIM GPDPTGVGS PLGVVPQTV VAQQKMPRS DRLEMDMKS VGSFYYDSF AFPAMMPYK RPAADKSGV PVYQPNATT YQQLMQLQQ PFVPVSCEY SVSPAPNPT TSCASPAAP PQSQQEITA ATTTVSQPA KENEVSSPV SSTASSAPP TTLPSLPLP QPTAIPDPA ALAKEVAQQ NYAKAVKLA AVSQSLAAG QLNQLNPLN YTGVALNKQ ALTVPPPAL TRYPTGALP FGLGTAGLN LGFANPYSM AQQNLLNIS RPPPAIINP YSIIRTSYP TANSTPLLG SGLLGTQYP VTVSAPPMT TVTASANIA AAAAQNNNN VVIQPYKKL KTT
- >TC014149 RC
MNNLLNGKD SRWLQLEVC REFQRNKCS RPDTECKFA HPPANVEVQ NGRVTACYD SIKGRCNRE KPPCKYFHP PQHLKDQLL INGRNHLAL KNALMQQMG LTPGQPLVP GQVQAVATN PYLTGMPQV GSTYSPYFA PGPIMPTIM GPDPTGVGS PLGVVPQTV VAQQKMPRS DRLEVCREY QRGACKRAE SECRFAHPA DSVTANEDG TVTVCMDAV KGRCNRDPC RYFHPPLHL QAQIKAAQS RASAPAAVS PLLAPPQAT AALEIGKKR PREPSTADT DLLLMDMKS VGSFYYDSF AFPAMMPYK RPAADKSGV PVYQPNATT YQQLMQLQQ PFVPVSCEY SVSPAPNPT TSCASPAAP PQSQQEITA ATTTVSQPA KENEVSSPV SSTASSAPP TTLPSLPLP QPTAIPDPA ALAKEVAQQ NYAKAVKLA AVSQSLAAG QLNQLNPLN YTGVALNKQ ALTVPPPAL TRYPTGALP FGLGTAGLN LGFANPYSM AQQNLLNIS RPPPAIINP YSIIRTSYP TANSTPLLG SGLLGTQYP VTVSAPPMT TVTASANIA AAAAQNNNN VVIQPYKKL KTT
- >TC014149 RA
atgaataat ttacttaat ggcaaggac tcccgctgg cttcagctt gaagtctgc agagaattc caaaggaac aaatgctcg agaccggac accgagtgt aaatttgca catccacca gccaacgtc gaggtccaa aacggccgc gtgacggcg tgctacgac agtattaag ggtcgctgc aacagagaa aaaccccca tgcaagtac ttccaccct ccacaacac ctcaaggac caacttctt ataaatggt aggaatcac ttggcgtta aaaaatgca cttatgcaa caaatgggt ctaacgccc ggacagcct ttggttcct ggtcaagtt caggcagtg gccacaaac ccctacctg actggaatg ccacaggtt ggcagtaca tatagtcca tattttgct ccgggtcct ataatgcct acgattatg gggccggat cctaccgga gtcgggtcg cctttgggt gtagttcca caaacagtt gtagcacaa caaaaaatg cctcgttcc gatagactt gaggtgtgc cgcgaatac cagcgcggg gcgtgcaag cgagcagag tcagaatgt cgctttgcg catcccgct gactctgta actgccaac gaggacggc actgtcact gtctgcatg gatgcagtc aagggccgc tgcaaccgc gacccctgc cgctatttc cacccccct ctgcacctc caagcccaa atcaaggcc gcacagtcg cgggctagc gcgatggat atgaaatct gttgggtca ttttattat gatagtttt gccttcccg gccatgatg ccgtacaag aggccagcg gcggacaaa tccggtgtt cctgtgtac caaccaaac gcaaccact taccaacaa ctgatgcag ttacagcag ccattcgtg cccgtgtca tgtgagtat tcggtctcc ccagctccg aatcccacc acctcctgc gcttctcct gctgctcct cctcaatca cagcaggaa atcacagca gcaacaaca acggtgtcg caaccagcc aaggagaac gaggtttcc agccccgtc tcctcgact gcttcgtct gcaccccca accacccta ccatctctg cctctgcct caacctact gccatacca gatcccgca gctcttgct aaggaggtc gctcaacag aattacgcc aaagccgtt aaactagcc gccgtaagt cagtcgtta gctgcaggc cagctgaac caactcaac cccctgaac tacacgggg gtcgccttg aacaagcaa gcgctgact gtgccccct cctgccttg acgcgttac ccaaccggc gccctgccg ttcggcttg ggcacggcg ggcctgaac ctgggtttt gcgaaccct tattcgatg gcccagcaa aatttgttg aacatttcg cgcccgccg ccggcgatt attaacccc tattcgatt atccgaact tcttatccc actgcgaac tcaacccct ctcttgggc agtggcctc ttgggcacg caataccct gtcactgtt tctgcaccg cctatgacg acagtcacc gcaagcgct aacatagcc gccgccgcg gcgcaaaat aacaataac gtggtcatt cagccttat aagaaactc aagactaca taa
- >TC014149 RB
atgaataat ttacttaat ggcaaggac tcccgctgg cttcagctt gaagtctgc agagaattc caaaggaac aaatgctcg agaccggac accgagtgt aaatttgca catccacca gccaacgtc gaggtccaa aacggccgc gtgacggcg tgctacgac agtattaag ggtcgctgc aacagagaa aaaccccca tgcaagtac ttccaccct ccacaacac ctcaaggac caacttctt ataaatggt aggaatcac ttggcgtta aaaaatgca cttatgcaa caaatgggt ctaacgccc ggacagcct ttggttcct ggtcaagtt caggcagtg gccacaaac ccctacctg actggaatg ccacaggtt ggcagtaca tatagtcca tattttgct ccgggtcct ataatgcct acgattatg gggccggat cctaccgga gtcgggtcg cctttgggt gtagttcca caaacagtt gtagcacaa caaaaaatg cctcgttcc gatagactt gagatggat atgaaatct gttgggtca ttttattat gatagtttt gccttcccg gccatgatg ccgtacaag aggccagcg gcggacaaa tccggtgtt cctgtgtac caaccaaac gcaaccact taccaacaa ctgatgcag ttacagcag ccattcgtg cccgtgtca tgtgagtat tcggtctcc ccagctccg aatcccacc acctcctgc gcttctcct gctgctcct cctcaatca cagcaggaa atcacagca gcaacaaca acggtgtcg caaccagcc aaggagaac gaggtttcc agccccgtc tcctcgact gcttcgtct gcaccccca accacccta ccatctctg cctctgcct caacctact gccatacca gatcccgca gctcttgct aaggaggtc gctcaacag aattacgcc aaagccgtt aaactagcc gccgtaagt cagtcgtta gctgcaggc cagctgaac caactcaac cccctgaac tacacgggg gtcgccttg aacaagcaa gcgctgact gtgccccct cctgccttg acgcgttac ccaaccggc gccctgccg ttcggcttg ggcacggcg ggcctgaac ctgggtttt gcgaaccct tattcgatg gcccagcaa aatttgttg aacatttcg cgcccgccg ccggcgatt attaacccc tattcgatt atccgaact tcttatccc actgcgaac tcaacccct ctcttgggc agtggcctc ttgggcacg caataccct gtcactgtt tctgcaccg cctatgacg acagtcacc gcaagcgct aacatagcc gccgccgcg gcgcaaaat aacaataac gtggtcatt cagccttat aagaaactc aagactaca taa
- >TC014149 RC
atgaataat ttacttaat ggcaaggac tcccgctgg cttcagctt gaagtctgc agagaattc caaaggaac aaatgctcg agaccggac accgagtgt aaatttgca catccacca gccaacgtc gaggtccaa aacggccgc gtgacggcg tgctacgac agtattaag ggtcgctgc aacagagaa aaaccccca tgcaagtac ttccaccct ccacaacac ctcaaggac caacttctt ataaatggt aggaatcac ttggcgtta aaaaatgca cttatgcaa caaatgggt ctaacgccc ggacagcct ttggttcct ggtcaagtt caggcagtg gccacaaac ccctacctg actggaatg ccacaggtt ggcagtaca tatagtcca tattttgct ccgggtcct ataatgcct acgattatg gggccggat cctaccgga gtcgggtcg cctttgggt gtagttcca caaacagtt gtagcacaa caaaaaatg cctcgttcc gatagactt gaggtgtgc cgcgaatac cagcgcggg gcgtgcaag cgagcagag tcagaatgt cgctttgcg catcccgct gactctgta actgccaac gaggacggc actgtcact gtctgcatg gatgcagtc aagggccgc tgcaaccgc gacccctgc cgctatttc cacccccct ctgcacctc caagcccaa atcaaggcc gcacagtcg cgggctagc gcgcccgct gccgtctcg cccttgctg gcgcccccc caggcaact gccgccctc gaaatcggc aagaagagg ccccgtgag cctagcacc gccgacaca gatctatta ctgatggat atgaaatct gttgggtca ttttattat gatagtttt gccttcccg gccatgatg ccgtacaag aggccagcg gcggacaaa tccggtgtt cctgtgtac caaccaaac gcaaccact taccaacaa ctgatgcag ttacagcag ccattcgtg cccgtgtca tgtgagtat tcggtctcc ccagctccg aatcccacc acctcctgc gcttctcct gctgctcct cctcaatca cagcaggaa atcacagca gcaacaaca acggtgtcg caaccagcc aaggagaac gaggtttcc agccccgtc tcctcgact gcttcgtct gcaccccca accacccta ccatctctg cctctgcct caacctact gccatacca gatcccgca gctcttgct aaggaggtc gctcaacag aattacgcc aaagccgtt aaactagcc gccgtaagt cagtcgtta gctgcaggc cagctgaac caactcaac cccctgaac tacacgggg gtcgccttg aacaagcaa gcgctgact gtgccccct cctgccttg acgcgttac ccaaccggc gccctgccg ttcggcttg ggcacggcg ggcctgaac ctgggtttt gcgaaccct tattcgatg gcccagcaa aatttgttg aacatttcg cgcccgccg ccggcgatt attaacccc tattcgatt atccgaact tcttatccc actgcgaac tcaacccct ctcttgggc agtggcctc ttgggcacg caataccct gtcactgtt tctgcaccg cctatgacg acagtcacc gcaagcgct aacatagcc gccgccgcg gcgcaaaat aacaataac gtggtcatt cagccttat aagaaactc aagactaca taa
- >TC014149 RA
tcacggcgg tccgacgtt cgcttgctt agtacactt tagtttaaa attttattt gtttatgtg tcctgctaa ccgtgtgct gtgtgaaat aactgtcgt cgtgccgtt cccgaatcc ttgtgaagc caccggaat ccttaacgc tttcaaaga ccgccaccg aagaagtta tctgaagac accggactt gcaatatgc gctagaacc gacaatcgc ttcgctttg cctacccgc ttctctgca aattattac tccgttcag aagcttttc tatacccgc cgacccatc agtaacaac gtcaataca gaaaacaca gaacaaaag agtacaaga acgcagtga cccaagatt atttttata tcactctct aacagtttt aagtaacgt tcgttaacg actcgttgt gttgttctt aaaacaatt tttcgaaag gatggacaa taattccac ctgacttga ttttgtgaa ccataacta tatttggag tgaatcgtt gcgcttcgt ccagtgttt ctcgcgtcc aatttgtac gaattattg acaaaaagg ccttcgcgc aatggcaat ggttaacat gaataattt acttaatgg caaggactc ccgctggct tcagcttga agtctgcag agaattcca aaggaacaa atgctcgag accggacac cgagtgtaa atttgcaca tccaccagc caacgtcga ggtccaaaa cggccgcgt gacggcgtg ctacgacag tattaaggg tcgctgcaa cagagaaaa acccccatg caagtactt ccaccctcc acaacacct caaggacca acttcttat aaatggtag gaatcactt ggcgttaaa aaatgcact tatgcaaca aatgggtct aacgcccgg acagccttt ggttcctgg tcaagttca ggcagtggc cacaaaccc ctacctgac tggaatgcc acaggttgg cagtacata tagtccata ttttgctcc gggtcctat aatgcctac gattatggg gccggatcc taccggagt cgggtcgcc tttgggtgt agttccaca aacagttgt agcacaaca aaaaatgcc tcgttccga tagacttga ggtgtgccg cgaatacca gcgcggggc gtgcaagcg agcagagtc agaatgtcg ctttgcgca tcccgctga ctctgtaac tgccaacga ggacggcac tgtcactgt ctgcatgga tgcagtcaa gggccgctg caaccgcga cccctgccg ctatttcca cccccctct gcacctcca agcccaaat caaggccgc acagtcgcg ggctagcgc gatggatat gaaatctgt tgggtcatt ttattatga tagttttgc cttcccggc catgatgcc gtacaagag gccagcggc ggacaaatc cggtgttcc tgtgtacca accaaacgc aaccactta ccaacaact gatgcagtt acagcagcc attcgtgcc cgtgtcatg tgagtattc ggtctcccc agctccgaa tcccaccac ctcctgcgc ttctcctgc tgctcctcc tcaatcaca gcaggaaat cacagcagc aacaacaac ggtgtcgca accagccaa ggagaacga ggtttccag ccccgtctc ctcgactgc ttcgtctgc acccccaac caccctacc atctctgcc tctgcctca acctactgc cataccaga tcccgcagc tcttgctaa ggaggtcgc tcaacagaa ttacgccaa agccgttaa actagccgc cgtaagtca gtcgttagc tgcaggcca gctgaacca actcaaccc cctgaacta cacgggggt cgccttgaa caagcaagc gctgactgt gccccctcc tgccttgac gcgttaccc aaccggcgc cctgccgtt cggcttggg cacggcggg cctgaacct gggttttgc gaaccctta ttcgatggc ccagcaaaa tttgttgaa catttcgcg cccgccgcc ggcgattat taaccccta ttcgattat ccgaacttc ttatcccac tgcgaactc aacccctct cttgggcag tggcctctt gggcacgca ataccctgt cactgtttc tgcaccgcc tatgacgac agtcaccgc aagcgctaa catagccgc cgccgcggc gcaaaataa caataacgt ggtcattca gccttataa gaaactcaa gactacata atttttaac cagttgctc gtcctagtt ttcagtgtt aattttgtg gaatatttt ggctgtgga tattgccat gtaaaattg aacgatgtt aaaaacatt tgcatttta ttgtttgtc gcagtgata tcttaattt acgttatgt gtatattta attgtgtta tctaaattg tggttttaa ttgaataaa taagtgtag ttata
- >TC014149 RB
tcacggcgg tccgacgtt cgcttgctt agtacactt tagtttaaa attttattt gtttatgtg tcctgctaa ccgtgtgct gtgtgaaat aactgtcgt cgtgccgtt cccgaatcc ttgtgaagc caccggaat ccttaacgc tttcaaaga ccgccaccg aagaagtta tctgaagac accggactt gcaatatgc gctagaacc gacaatcgc ttcgctttg cctacccgc ttctctgca aattattac tccgttcag aagcttttc tatacccgc cgacccatc agtaacaac gtcaataca gaaaacaca gaacaaaag agtacaaga acgcagtga cccaagatt atttttata tcactctct aacagtttt aagtaacgt tcgttaacg actcgttgt gttgttctt aaaacaatt tttcgaaag gatggacaa taattccac ctgacttga ttttgtgaa ccataacta tatttggag tgaatcgtt gcgcttcgt ccagtgttt ctcgcgtcc aatttgtac gaattattg acaaaaagg ccttcgcgc aatggcaat ggttaacat gaataattt acttaatgg caaggactc ccgctggct tcagcttga agtctgcag agaattcca aaggaacaa atgctcgag accggacac cgagtgtaa atttgcaca tccaccagc caacgtcga ggtccaaaa cggccgcgt gacggcgtg ctacgacag tattaaggg tcgctgcaa cagagaaaa acccccatg caagtactt ccaccctcc acaacacct caaggacca acttcttat aaatggtag gaatcactt ggcgttaaa aaatgcact tatgcaaca aatgggtct aacgcccgg acagccttt ggttcctgg tcaagttca ggcagtggc cacaaaccc ctacctgac tggaatgcc acaggttgg cagtacata tagtccata ttttgctcc gggtcctat aatgcctac gattatggg gccggatcc taccggagt cgggtcgcc tttgggtgt agttccaca aacagttgt agcacaaca aaaaatgcc tcgttccga tagacttga gatggatat gaaatctgt tgggtcatt ttattatga tagttttgc cttcccggc catgatgcc gtacaagag gccagcggc ggacaaatc cggtgttcc tgtgtacca accaaacgc aaccactta ccaacaact gatgcagtt acagcagcc attcgtgcc cgtgtcatg tgagtattc ggtctcccc agctccgaa tcccaccac ctcctgcgc ttctcctgc tgctcctcc tcaatcaca gcaggaaat cacagcagc aacaacaac ggtgtcgca accagccaa ggagaacga ggtttccag ccccgtctc ctcgactgc ttcgtctgc acccccaac caccctacc atctctgcc tctgcctca acctactgc cataccaga tcccgcagc tcttgctaa ggaggtcgc tcaacagaa ttacgccaa agccgttaa actagccgc cgtaagtca gtcgttagc tgcaggcca gctgaacca actcaaccc cctgaacta cacgggggt cgccttgaa caagcaagc gctgactgt gccccctcc tgccttgac gcgttaccc aaccggcgc cctgccgtt cggcttggg cacggcggg cctgaacct gggttttgc gaaccctta ttcgatggc ccagcaaaa tttgttgaa catttcgcg cccgccgcc ggcgattat taaccccta ttcgattat ccgaacttc ttatcccac tgcgaactc aacccctct cttgggcag tggcctctt gggcacgca ataccctgt cactgtttc tgcaccgcc tatgacgac agtcaccgc aagcgctaa catagccgc cgccgcggc gcaaaataa caataacgt ggtcattca gccttataa gaaactcaa gactacata atttttaac cagttgctc gtcctagtt ttcagtgtt aattttgtg gaatatttt ggctgtgga tattgccat gtaaaattg aacgatgtt aaaaacatt tgcatttta ttgtttgtc gcagtgata tcttaattt acgttatgt gtatattta attgtgtta tctaaattg tggttttaa ttgaataaa taagtgtag ttata
- >TC014149 RC
accagtctg tgtgtcagt ttcgagaga cgcacatat acactaaac actctgtat actgttcac aacgtataa aaattacaa ttaatgtat tttaaataa aattacagt ctccaattt gctatctag cgtgctgtt ctatacaac gacacaaat ttgcaaggt gtctgaatt gctttttac agtgaacca aaaatattt ttccaagtg cgtccgaag aacgtctag gacaccgcc gggagtttg aaatttcgt aaacatact tggagtaca ttattttgc tgtggaaat tttttgttc agccaatac cgtaacgat agctttctg cctggtttc ttaattttt ggtaaaaca ccggattaa atagttctg gaatgcgtg cttaatttt tgtttggtg gttagtgat tttgctgga gccggtgct gctgcaaaa accgtttcg tctggattt cttataccg ccaccgaag aagttatct gaagacacc ggacttgca atatgcgct agaaccgac aatcgcttc gctttgcct acccgcttc tctgcaaat tattactcc gttcagaag cttttctat acccgccga cccatcagt aacaacgtc aatacagaa aacacagaa caaaagagt acaagaacg cagtgaccc aagattatt tttatatca ctctctaac agttttaag taacgttcg ttaacgact cgttgtgtt gttcttaaa acaattttt cgaaaggat ggacaataa ttccacctg acttgattt tgtgaacca taactatat ttggagtga atcgttgcg cttcgtcca gtgtttctc gcgtccaat ttgtacgaa ttattgaca aaaaggcct tcgcgcaat ggcaatggt taacatgaa taatttact taatggcaa ggactcccg ctggcttca gcttgaagt ctgcagaga attccaaag gaacaaatg ctcgagacc ggacaccga gtgtaaatt tgcacatcc accagccaa cgtcgaggt ccaaaacgg ccgcgtgac ggcgtgcta cgacagtat taagggtcg ctgcaacag agaaaaacc cccatgcaa gtacttcca ccctccaca acacctcaa ggaccaact tcttataaa tggtaggaa tcacttggc gttaaaaaa tgcacttat gcaacaaat gggtctaac gcccggaca gcctttggt tcctggtca agttcaggc agtggccac aaaccccta cctgactgg aatgccaca ggttggcag tacatatag tccatattt tgctccggg tcctataat gcctacgat tatggggcc ggatcctac cggagtcgg gtcgccttt gggtgtagt tccacaaac agttgtagc acaacaaaa aatgcctcg ttccgatag acttgaggt gtgccgcga ataccagcg cggggcgtg caagcgagc agagtcaga atgtcgctt tgcgcatcc cgctgactc tgtaactgc caacgagga cggcactgt cactgtctg catggatgc agtcaaggg ccgctgcaa ccgcgaccc ctgccgcta tttccaccc ccctctgca cctccaagc ccaaatcaa ggccgcaca gtcgcgggc tagcgcgcc cgctgccgt ctcgccctt gctggcgcc cccccaggc aactgccgc cctcgaaat cggcaagaa gaggccccg tgagcctag caccgccga cacagatct attactgat ggatatgaa atctgttgg gtcatttta ttatgatag ttttgcctt cccggccat gatgccgta caagaggcc agcggcgga caaatccgg tgttcctgt gtaccaacc aaacgcaac cacttacca acaactgat gcagttaca gcagccatt cgtgcccgt gtcatgtga gtattcggt ctccccagc tccgaatcc caccacctc ctgcgcttc tcctgctgc tcctcctca atcacagca ggaaatcac agcagcaac aacaacggt gtcgcaacc agccaagga gaacgaggt ttccagccc cgtctcctc gactgcttc gtctgcacc cccaaccac cctaccatc tctgcctct gcctcaacc tactgccat accagatcc cgcagctct tgctaagga ggtcgctca acagaatta cgccaaagc cgttaaact agccgccgt aagtcagtc gttagctgc aggccagct gaaccaact caaccccct gaactacac gggggtcgc cttgaacaa gcaagcgct gactgtgcc ccctcctgc cttgacgcg ttacccaac cggcgccct gccgttcgg cttgggcac ggcgggcct gaacctggg ttttgcgaa cccttattc gatggccca gcaaaattt gttgaacat ttcgcgccc gccgccggc gattattaa cccctattc gattatccg aacttctta tcccactgc gaactcaac ccctctctt gggcagtgg cctcttggg cacgcaata ccctgtcac tgtttctgc accgcctat gacgacagt caccgcaag cgctaacat agccgccgc cgcggcgca aaataacaa taacgtggt cattcagcc ttataagaa actcaagac tacataatt tttaaccag ttgctcgtc ctagttttc agtgttaat tttgtggaa tattttggc tgtggatat tgccatgta aaattgaac gatgttaaa aacatttgc attttattg tttgtcgca gtgatatct taatttacg ttatgtgta tatttaatt gtgttatct aaattgtgg ttttaattg aataaataa gtgtagtta ta
