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Gene information
General information
TC033082
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014139
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6499280..6505385
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033082
    iBeetle screen iB_05567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033082 RA
      MFKGTRLAA TPVYASVAI EEVLLGTAA TPAFVTVLQ 
      QITPFQVQD KLEDLPVLP KAATKEAQE LVKDLSQRC 
      SKSKNKDAK ELFDLLSTP HFKSLIDCH DQVARISEN 
      PPKPVKTDI SKLFPALNG MTGEAIRMV GVRKKDGEP 
      LGLTVQVDD NENLVIARI LEGGMIEKQ GLLHVGDVI 
      LEVNGTPVK SAEDLQIEV AKSKDSVTL KVGQPSEME 
      THASLVMNG TSNGVAKKL TCYMRALFE YIPEEDSLL 
      PCKEIGLPF DRGDILQIV DQSDPNWWQ AKKVDGDGR 
      TGLIPSLEL EERRKAFVA PEADFVHKI SICGARISK 
      KKKKIIYQS KSNCDFDKA ELLLYEEVT RMPPFKRKT 
      LVLIGTQGV GRRTLKNRL INSDPDKFG GVVPYTTRP 
      QRVLEENGQ SYWFTDRES MEEDIKHSK FLEYGEYNG 
      HLYGTHLDS IREVIKQGK MCVLDCSPI ALKILHNSS 
      EFLPYVIFI AAPGMEQLK ILYDVGRSS SNLRYSSRN 
      LTFDRQSSI RYSSRRART LESLASLYE EEDLKRTLE 
      ESASMQRTY DKYIDQVIV NNDFDTTFR QIVEALDTL 
      TTEHQWVPV NWIY
    CDS sequence
    • >TC033082 RA
      atgtttaaa ggtacgcgg ctcgcagcc acacctgta 
      tacgcgagt gtggcaatt gaggaggta cttcttggc 
      acagcggca acaccggcc tttgtgacc gtactacaa 
      caaataacc ccctttcag gttcaagac aaattagag 
      gatcttcca gtgcttccg aaagcagct acgaaagaa 
      gcccaagaa ctggtaaag gatttgtcc cagcgctgc 
      agtaaatca aaaaataag gacgccaaa gaactattt 
      gacttgcta tcgacgcct cattttaaa tccctgatt 
      gattgtcac gaccaagtc gcgcgtata agcgaaaat 
      cccccaaag cccgtaaag actgacatt tcgaaatta 
      ttcccagca ttaaacggg atgacggga gaggcgata 
      aggatggtg ggtgtccgc aaaaaggac ggggagccc 
      ttgggcttg acagtgcag gtggatgac aacgaaaat 
      ttggtaata gcaaggata ctagaagga gggatgatc 
      gaaaaacag ggcctcctc cacgttggt gatgttatc 
      ttagaagtc aatggaacg ccagtaaag agcgctgaa 
      gacctgcaa attgaagtg gcaaagtcg aaggattca 
      gtgaccctc aaagttggc caacccagc gaaatggaa 
      actcacgct tcactcgtt atgaacgga accagtaac 
      ggagtcgcc aaaaagctc acatgctac atgagagct 
      ctgttcgag tacatcccc gaggaagac agcctcctt 
      ccttgtaaa gaaataggt ctgcccttc gatcggggt 
      gacatcctc cagattgtg gaccagagt gatcccaac 
      tggtggcag gcaaagaaa gtggacggg gatggcaga 
      actggttta attccgtct ctggaactg gaggaaagg 
      aggaaggca ttcgtggca cccgaagcc gattttgta 
      cataaaata agtatatgt ggtgcgaga atttcgaag 
      aaaaagaag aaaatcatt tatcagtcc aaaagcaat 
      tgtgatttc gataaggct gagcttttg ctgtatgaa 
      gaagtgaca agaatgccg ccgtttaag cgaaaaact 
      ttagtcctg atcggaact caaggtgtt ggaaggaga 
      acactgaag aatcgcttg attaatagt gaccctgat 
      aagtttggc ggagttgta ccatataca acgaggcca 
      caacgcgtt ttggaggaa aacggccaa agttattgg 
      tttactgac cgcgagagc atggaggag gacataaaa 
      cactcaaag tttttggag tatggtgaa tacaatggt 
      cacttgtac ggaacacat ctcgattct atcagagaa 
      gtaattaag cagggaaaa atgtgtgtc ttggactgc 
      agtccaatc gcgctcaaa attctgcat aacagctca 
      gagtttcta ccttatgtc atatttatc gcggctcca 
      ggaatggag caacttaaa attttatac gacgtgggg 
      cgcagcagc tctaatttg cgatactcg agccggaat 
      ctaacattt gatcgccaa agctcgata cgctacagt 
      tccaggagg gcaaggaca ctagagtcg ttagcctct 
      ctgtatgaa gaggaagat ctgaagcga acacttgag 
      gaaagcgcc agcatgcaa cgaacgtac gataagtac 
      attgaccag gttatcgtc aacaatgat tttgataca 
      actttccga caaattgtg gaggccttg gatacttta 
      accactgag catcagtgg gtaccggtt aattggatt 
      tattga
    mRNA sequence
    • >TC033082 RA
      ctgtgtatc agaaaccga ccaagttgg ttcgcgggt 
      cgcgtattt tataacgta cctaaaaaa cacctgcgt 
      ctggtgcct ttaaacgcc cttttagct ttgtgtatc 
      tagacggaa ccgtttata agaattttg tgttaatcc 
      ctgtggtgg ctgtgctaa tattttatg ttaatttaa 
      cgtaaaata tgtgaactt tctatcagt ggcgttcga 
      ttgttgatg ttgatggtg acgtcactg cttattatg 
      tgtttgctt acttcgtat tttgattga aaactaggt 
      aatgagagc ttttttgca tcgtactat tttttctat 
      tattgtctg tcgtgatgt atgaactaa agatttcgc 
      cacatatgc attttgtta aatacttgg tatgtgaat 
      catgtttaa aggtacgcg gctcgcagc cacacctgt 
      atacgcgag tgtggcaat tgaggaggt acttcttgg 
      cacagcggc aacaccggc ctttgtgac cgtactaca 
      acaaataac cccctttca ggttcaaga caaattaga 
      ggatcttcc agtgcttcc gaaagcagc tacgaaaga 
      agcccaaga actggtaaa ggatttgtc ccagcgctg 
      cagtaaatc aaaaaataa ggacgccaa agaactatt 
      tgacttgct atcgacgcc tcattttaa atccctgat 
      tgattgtca cgaccaagt cgcgcgtat aagcgaaaa 
      tcccccaaa gcccgtaaa gactgacat ttcgaaatt 
      attcccagc attaaacgg gatgacggg agaggcgat 
      aaggatggt gggtgtccg caaaaagga cggggagcc 
      cttgggctt gacagtgca ggtggatga caacgaaaa 
      tttggtaat agcaaggat actagaagg agggatgat 
      cgaaaaaca gggcctcct ccacgttgg tgatgttat 
      cttagaagt caatggaac gccagtaaa gagcgctga 
      agacctgca aattgaagt ggcaaagtc gaaggattc 
      agtgaccct caaagttgg ccaacccag cgaaatgga 
      aactcacgc ttcactcgt tatgaacgg aaccagtaa 
      cggagtcgc caaaaagct cacatgcta catgagagc 
      tctgttcga gtacatccc cgaggaaga cagcctcct 
      tccttgtaa agaaatagg tctgccctt cgatcgggg 
      tgacatcct ccagattgt ggaccagag tgatcccaa 
      ctggtggca ggcaaagaa agtggacgg ggatggcag 
      aactggttt aattccgtc tctggaact ggaggaaag 
      gaggaaggc attcgtggc acccgaagc cgattttgt 
      acataaaat aagtatatg tggtgcgag aatttcgaa 
      gaaaaagaa gaaaatcat ttatcagtc caaaagcaa 
      ttgtgattt cgataaggc tgagctttt gctgtatga 
      agaagtgac aagaatgcc gccgtttaa gcgaaaaac 
      tttagtcct gatcggaac tcaaggtgt tggaaggag 
      aacactgaa gaatcgctt gattaatag tgaccctga 
      taagtttgg cggagttgt accatatac aacgaggcc 
      acaacgcgt tttggagga aaacggcca aagttattg 
      gtttactga ccgcgagag catggagga ggacataaa 
      acactcaaa gtttttgga gtatggtga atacaatgg 
      tcacttgta cggaacaca tctcgattc tatcagaga 
      agtaattaa gcagggaaa aatgtgtgt cttggactg 
      cagtccaat cgcgctcaa aattctgca taacagctc 
      agagtttct accttatgt catatttat cgcggctcc 
      aggaatgga gcaacttaa aattttata cgacgtggg 
      gcgcagcag ctctaattt gcgatactc gagccggaa 
      tctaacatt tgatcgcca aagctcgat acgctacag 
      ttccaggag ggcaaggac actagagtc gttagcctc 
      tctgtatga agaggaaga tctgaagcg aacacttga 
      ggaaagcgc cagcatgca acgaacgta cgataagta 
      cattgacca ggttatcgt caacaatga ttttgatac 
      aactttccg acaaattgt ggaggcctt ggatacttt 
      aaccactga gcatcagtg ggtaccggt taattggat 
      ttattgata ttctactgc catatatta taaggaatc 
      tctctgtaa ttctaggtt tttataaag tatcttcac 
      gaaatgttt tatctgaaa agtctcagt attaattag 
      atattggaa gggttgttc tgaacaagc ccttgttgt 
      aatatatcg aaacgctcg ttgattggt tattaaaaa 
      tatggaagt taagacatt ttcttgtag atataagtt 
      cttttaaaa tgtgaaagt actgttagc tttaatcct 
      gttttgttt attagcaca ctagcctga aatcaatat 
      tatattaca tttataaaa agcatcaca agtagatta 
      gttaaatac gacactgtt ggtagtgat atctagctg 
      ttccggaga acttgagtc gagattttt acatgaaag 
      aaggtacaa agttctgca acatcgaat aattacttc 
      tcacagatt agtcagtaa gtaagcctt ataatgcct 
      aactattat taagtgtac atatttgaa ctgagcaaa 
      gatatgagt cacccgtac tatgaagta gactgcaga 
      acgagttca aatttattt gaaaaactc ttctgcacc 
      tagatagat tactagaat tagattgtt tgttatata 
      ggtgcttta tattgtatc gttttaacc gtccaagtg 
      ttcagggaa atgtgtttt gatgagatt acttatcta 
      tattcaatg atcttttgc aattatata catttcttt 
      atacatact tacacttac atcatactt aggtataca 
      gttggtagt tttaatgtt taaaatatt gttttcaaa 
      tatgatttt tatttagtt gtgacaatt tttaatttg 
      tagtttatt cagtttcgc aaataaata tagaatttt 
      aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY