Status
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Gene information
General information
TC014135 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6449042..6469706
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014135
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05566

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50
    about 50
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014135 RA
      MLMFESPAA HHHKEVLPT KYGSKPPTT ASGKHLNKS 
      SSKNGHSTS KTTLISELP LLESSKSSS RLNLNQSKT 
      NLTPSSPMV DSFPAINNN SRTVEIQGT TNNNRENNG 
      FPMTPTEAL KLYGNRLTE YEKNEIEKY SKIWYLGLN 
      ACKIHGEVG GSQNCGYDD ESGSYIKVL HDHICYRYE 
      ILEVIGKGS FGQVIRALD HMTNKHVAI KIIRNKKRF 
      QHPALVEVR ILDHLRKKD KDGNHNVIH MSEHFTFRN 
      HLCISFELL SLNLFELLK KNSYRGFSL NLIRRFASS 
      ILKCLRLLQ KENIIHCDL KPENVLLKE RGSTSIRVI 
      DFGSSCYTN QRVYTYIQS RFYRSPEVI LGLPYGTPI 
      DMWSFGCIL AELYTGYPL FPGENEVEQ LACLMEVLG 
      QPPDELIVN ATRKRLFFD SRGNPRCIT NSKGKKRKP 
      GSKNLSTAL HCDDPLFMD FISRCLEWN AKKRMTPDE 
      ALRHEWILS GTSGYQKSG NDLRHSHSE VNVGHESYS 
      SSSTSGSSH SRIYHKNIA PKGDKVKAK ILDTRLHDS 
      ISKLDSNLN DSGTFLPPI L
    • >TC014135 RB
      MKRSRSVME ETTMRDLRV LIVGNAGHS IAQAMALVC 
      DLISIGSGA ASWCVVSAC HGLGNPSGP RPLMCAYHT 
      YPSTRGSRT MGWTHADHS SPAAHHHKE VLPTKYGSK 
      PPTTASGKH LNKSSSKNG HSTSKTTLI SELPLLESS 
      KSSSRLNLN QSKTNLTPS SPMVDSFPA INNNSRTVE 
      IQGTTNNNR ENNGFPMTP TEALKLYGN RLTEYEKNE 
      IEKYSKIWY LGLNACKIH GEVGGSQNC GYDDESGSY 
      IKVLHDHIC YRYEILEVI GKGSFGQVI RALDHMTNK 
      HVAIKIIRN KKRFQHPAL VEVRILDHL RKKDKDGNH 
      NVIHMSEHF TFRNHLCIS FELLSLNLF ELLKKNSYR 
      GFSLNLIRR FASSILKCL RLLQKENII HCDLKPENV 
      LLKERGSTS IRVIDFGSS CYTNQRVYT YIQSRFYRS 
      PEVILGLPY GTPIDMWSF GCILAELYT GYPLFPGEN 
      EVEQLACLM EVLGQPPDE LIVNATRKR LFFDSRGNP 
      RCITNSKGK KRKPGSKNL STALHCDDP LFMDFISRC 
      LEWNAKKRM TPDEALRHE WILSGTSGY QKSGNDLRH 
      SHSEVNVGH ESYSSSSTS GSSHSRIYH KNIAPKGDK 
      VKAKILDTR LHDSISKLD SNLNDSGTF LPPIL
    • >TC014135 RC
      MKRSRSVME ETTMRDLRV LIVGNAGHS IAQAMALVC 
      DLISIGSGA ASWCVVSAC HGLGNPSGP RPLMCAYHT 
      YPSTRGSRT MGWTHADHS VFHTISPAA HHHKEVLPT 
      KYGSKPPTT ASGKHLNKS SSKNGHSTS KTTLISELP 
      LLESSKSSS RLNLNQSKT NLTPSSPMV DSFPAINNN 
      SRTVEIQGT TNNNRENNG FPMTPTEAL KLYGNRLTE 
      YEKNEIEKY SKIWYLGLN ACKIHGEVG GSQNCGYDD 
      ESGSYIKVL HDHICYRYE ILEVIGKGS FGQVIRALD 
      HMTNKHVAI KIIRNKKRF QHPALVEVR ILDHLRKKD 
      KDGNHNVIH MSEHFTFRN HLCISFELL SLNLFELLK 
      KNSYRGFSL NLIRRFASS ILKCLRLLQ KENIIHCDL 
      KPENVLLKE RGSTSIRVI DFGSSCYTN QRVYTYIQS 
      RFYRSPEVI LGLPYGTPI DMWSFGCIL AELYTGYPL 
      FPGENEVEQ LACLMEVLG QPPDELIVN ATRKRLFFD 
      SRGNPRCIT NSKGKKRKP GSKNLSTAL HCDDPLFMD 
      FISRCLEWN AKKRMTPDE ALRHEWILS GTSGYQKSG 
      NDLRHSHSE VNVGHESYS SSSTSGSSH SRIYHKNIA 
      PKGDKVKAK ILDTRLHDS ISKLDSNLN DSGTFLPPI 
      L
    CDS sequence
    • >TC014135 RA
      atgctcatg ttcgagagt ccagcagct caccaccat 
      aaagaagta ttaccgaca aagtatggg tccaagcca 
      ccgacgact gcatccggc aagcacttg aataaatct 
      tcttccaaa aatggtcat tcaacatcg aaaactacg 
      cttatttcc gaattgccg ttattggaa agcagcaaa 
      tcgtcttcg cgactcaac ctgaaccaa agcaagaca 
      aatttgaca ccgagctca ccgatggtt gattcattc 
      ccggctatc aataataat tcacgaact gtggaaatt 
      caagggacg acaaacaac aacagggag aataatggc 
      tttcctatg acaccaaca gaagctctt aaactgtat 
      gggaaccgt ttgacagag tacgagaag aatgaaata 
      gagaagtat tccaagatt tggtatttg ggcttaaat 
      gcttgtaaa attcatggc gaagttggg ggttcgcaa 
      aactgtggt tacgacgac gaaagtggg agctacatc 
      aaggttctg cacgatcac atctgctac cgatacgaa 
      atcctggaa gttattgga aagggtagc tttgggcaa 
      gtcatcagg gcgctcgat cacatgacc aataagcat 
      gtagcgatc aaaatcata aggaacaag aagcggttc 
      caacatccg gcactggta gaagtccga atactggat 
      catttgcga aaaaaagac aaagatgga aatcacaac 
      gtcatccac atgtctgaa catttcacc tttagaaat 
      cacctctgc ataagtttc gagttacta agccttaat 
      cttttcgaa ctgctgaag aaaaacagc taccgtgga 
      tttagtcta aatttaatc agaaggttt gccagttcc 
      attctcaaa tgtctgagg cttctccag aaggaaaac 
      atcatacac tgcgatctt aaaccggaa aatgttctg 
      ctgaaggaa aggggaagt acttcgata agagtaata 
      gatttcgga agttcgtgt tacacaaac caacgggtt 
      tacacttac atccagtct cgtttttat cgatcaccg 
      gaagtcata ttggggttg ccctatggg acgcctatt 
      gatatgtgg agttttggc tgtatcttg gccgagttg 
      tacacgggt tatccatta tttcctggc gaaaatgaa 
      gtcgagcaa cttgcgtgc ttgatggaa gttttggga 
      cagccacct gacgagctc attgtcaac gctactaga 
      aagagatta tttttcgat tcccggggg aaccccaga 
      tgcatcaca aactccaag ggcaagaaa cgcaagcca 
      ggatcgaag aacttgtcg acagcactg cactgtgat 
      gatcccttg tttatggac ttcatatcc aggtgtctt 
      gagtggaac gccaagaag agaatgaca ccagatgaa 
      gcgttgcgc cacgaatgg attctctcg ggcacttcg 
      ggctaccaa aagtctgga aatgactta cgacacagc 
      cattctgaa gtaaacgta ggccacgag agttattcg 
      tctagcagt acttccggc agcagccat tcccgcatt 
      tatcataaa aatatcgct cccaagggt gataaagtc 
      aaggccaaa attttagat actagatta catgatagt 
      atttctaaa ctagattca aacttgaac gattcggga 
      acttttctt cccccgatt ctttag
    • >TC014135 RB
      atgaaaaga agcagatcg gtgatggag gagacgacc 
      atgcgcgat ctccgcgta ttgatcgtc gggaacgcg 
      ggccacagc atcgctcag gcaatggca ttagtttgc 
      gacctcatc tcaataggg tccggtgcc gcctcctgg 
      tgcgtagtg agtgcgtgt catgggctc ggaaacccc 
      agcggcccg cggcccttg atgtgtgcc taccacacg 
      taccccagc acccggggg tcccgaact atgggctgg 
      actcacgct gaccactcg agtccagca gctcaccac 
      cataaagaa gtattaccg acaaagtat gggtccaag 
      ccaccgacg actgcatcc ggcaagcac ttgaataaa 
      tcttcttcc aaaaatggt cattcaaca tcgaaaact 
      acgcttatt tccgaattg ccgttattg gaaagcagc 
      aaatcgtct tcgcgactc aacctgaac caaagcaag 
      acaaatttg acaccgagc tcaccgatg gttgattca 
      ttcccggct atcaataat aattcacga actgtggaa 
      attcaaggg acgacaaac aacaacagg gagaataat 
      ggctttcct atgacacca acagaagct cttaaactg 
      tatgggaac cgtttgaca gagtacgag aagaatgaa 
      atagagaag tattccaag atttggtat ttgggctta 
      aatgcttgt aaaattcat ggcgaagtt gggggttcg 
      caaaactgt ggttacgac gacgaaagt gggagctac 
      atcaaggtt ctgcacgat cacatctgc taccgatac 
      gaaatcctg gaagttatt ggaaagggt agctttggg 
      caagtcatc agggcgctc gatcacatg accaataag 
      catgtagcg atcaaaatc ataaggaac aagaagcgg 
      ttccaacat ccggcactg gtagaagtc cgaatactg 
      gatcatttg cgaaaaaaa gacaaagat ggaaatcac 
      aacgtcatc cacatgtct gaacatttc acctttaga 
      aatcacctc tgcataagt ttcgagtta ctaagcctt 
      aatcttttc gaactgctg aagaaaaac agctaccgt 
      ggatttagt ctaaattta atcagaagg tttgccagt 
      tccattctc aaatgtctg aggcttctc cagaaggaa 
      aacatcata cactgcgat cttaaaccg gaaaatgtt 
      ctgctgaag gaaagggga agtacttcg ataagagta 
      atagatttc ggaagttcg tgttacaca aaccaacgg 
      gtttacact tacatccag tctcgtttt tatcgatca 
      ccggaagtc atattgggg ttgccctat gggacgcct 
      attgatatg tggagtttt ggctgtatc ttggccgag 
      ttgtacacg ggttatcca ttatttcct ggcgaaaat 
      gaagtcgag caacttgcg tgcttgatg gaagttttg 
      ggacagcca cctgacgag ctcattgtc aacgctact 
      agaaagaga ttatttttc gattcccgg gggaacccc 
      agatgcatc acaaactcc aagggcaag aaacgcaag 
      ccaggatcg aagaacttg tcgacagca ctgcactgt 
      gatgatccc ttgtttatg gacttcata tccaggtgt 
      cttgagtgg aacgccaag aagagaatg acaccagat 
      gaagcgttg cgccacgaa tggattctc tcgggcact 
      tcgggctac caaaagtct ggaaatgac ttacgacac 
      agccattct gaagtaaac gtaggccac gagagttat 
      tcgtctagc agtacttcc ggcagcagc cattcccgc 
      atttatcat aaaaatatc gctcccaag ggtgataaa 
      gtcaaggcc aaaatttta gatactaga ttacatgat 
      agtatttct aaactagat tcaaacttg aacgattcg 
      ggaactttt cttcccccg attctttag
    • >TC014135 RC
      atgaaaaga agcagatcg gtgatggag gagacgacc 
      atgcgcgat ctccgcgta ttgatcgtc gggaacgcg 
      ggccacagc atcgctcag gcaatggca ttagtttgc 
      gacctcatc tcaataggg tccggtgcc gcctcctgg 
      tgcgtagtg agtgcgtgt catgggctc ggaaacccc 
      agcggcccg cggcccttg atgtgtgcc taccacacg 
      taccccagc acccggggg tcccgaact atgggctgg 
      actcacgct gaccactcg gtattccat acaattagt 
      ccagcagct caccaccat aaagaagta ttaccgaca 
      aagtatggg tccaagcca ccgacgact gcatccggc 
      aagcacttg aataaatct tcttccaaa aatggtcat 
      tcaacatcg aaaactacg cttatttcc gaattgccg 
      ttattggaa agcagcaaa tcgtcttcg cgactcaac 
      ctgaaccaa agcaagaca aatttgaca ccgagctca 
      ccgatggtt gattcattc ccggctatc aataataat 
      tcacgaact gtggaaatt caagggacg acaaacaac 
      aacagggag aataatggc tttcctatg acaccaaca 
      gaagctctt aaactgtat gggaaccgt ttgacagag 
      tacgagaag aatgaaata gagaagtat tccaagatt 
      tggtatttg ggcttaaat gcttgtaaa attcatggc 
      gaagttggg ggttcgcaa aactgtggt tacgacgac 
      gaaagtggg agctacatc aaggttctg cacgatcac 
      atctgctac cgatacgaa atcctggaa gttattgga 
      aagggtagc tttgggcaa gtcatcagg gcgctcgat 
      cacatgacc aataagcat gtagcgatc aaaatcata 
      aggaacaag aagcggttc caacatccg gcactggta 
      gaagtccga atactggat catttgcga aaaaaagac 
      aaagatgga aatcacaac gtcatccac atgtctgaa 
      catttcacc tttagaaat cacctctgc ataagtttc 
      gagttacta agccttaat cttttcgaa ctgctgaag 
      aaaaacagc taccgtgga tttagtcta aatttaatc 
      agaaggttt gccagttcc attctcaaa tgtctgagg 
      cttctccag aaggaaaac atcatacac tgcgatctt 
      aaaccggaa aatgttctg ctgaaggaa aggggaagt 
      acttcgata agagtaata gatttcgga agttcgtgt 
      tacacaaac caacgggtt tacacttac atccagtct 
      cgtttttat cgatcaccg gaagtcata ttggggttg 
      ccctatggg acgcctatt gatatgtgg agttttggc 
      tgtatcttg gccgagttg tacacgggt tatccatta 
      tttcctggc gaaaatgaa gtcgagcaa cttgcgtgc 
      ttgatggaa gttttggga cagccacct gacgagctc 
      attgtcaac gctactaga aagagatta tttttcgat 
      tcccggggg aaccccaga tgcatcaca aactccaag 
      ggcaagaaa cgcaagcca ggatcgaag aacttgtcg 
      acagcactg cactgtgat gatcccttg tttatggac 
      ttcatatcc aggtgtctt gagtggaac gccaagaag 
      agaatgaca ccagatgaa gcgttgcgc cacgaatgg 
      attctctcg ggcacttcg ggctaccaa aagtctgga 
      aatgactta cgacacagc cattctgaa gtaaacgta 
      ggccacgag agttattcg tctagcagt acttccggc 
      agcagccat tcccgcatt tatcataaa aatatcgct 
      cccaagggt gataaagtc aaggccaaa attttagat 
      actagatta catgatagt atttctaaa ctagattca 
      aacttgaac gattcggga acttttctt cccccgatt 
      ctttag
    mRNA sequence
    • >TC014135 RA
      ggtcaaaaa tatcagcag ggtccagtt ataacgtac 
      agaaaaaat aaaataaat aagataaaa aaattaaat 
      aaaacgact gacatacgc accctaaaa aatgttttg 
      gatgttaat gctgttata gaaaacatg gtcttattg 
      tcaaatatt tattcttcc gaattttgt ttatttaat 
      tacccaatt aaagattca ctacttgta ggtcaaagc 
      tccccagtt cgctcgcat caaaatatt actattgta 
      tgaggggat gcaactttt ataataaac cacttaaaa 
      gctctccgt tgcaggtcg agctttaaa cattcggtc 
      ttgatacaa atactgata ataataaaa atcagatta 
      gttacttgt ctcaatgag agcccacac taatttatt 
      gttttattg ttatgttaa tgttaataa catagcgtt 
      ccgaagtac agcaaacac acaagaatt tcttcatgg 
      aacaaacac tgaagataa tagcttatc gaagtaaag 
      aaaaattca aacaaggcc ataatcccc gaggaaatt 
      acaacccag gtcgcgtag ctttgagtg ctttgacag 
      atcttcaat ccagacaag gcaccgtac agctcgaat 
      accatcagt ggtcttccc cacacatac ctacgccaa 
      agagtgaat gatccgata tcatcgtgt ctggaggat 
      catcacact cacacgatc gcactcgca tttctcgat 
      agtcggtgt cggaaagtg cgcgtgttg actggggac 
      tagcgcgca ctgtcgtca actttccca ttgttcgtt 
      agctctttg aattttgaa aatttttgg tcagctgag 
      tgatacatg tgaacattt tgccaaaat gctcatgtt 
      cgagagtcc agcagctca ccaccataa agaagtatt 
      accgacaaa gtatgggtc caagccacc gacgactgc 
      atccggcaa gcacttgaa taaatcttc ttccaaaaa 
      tggtcattc aacatcgaa aactacgct tatttccga 
      attgccgtt attggaaag cagcaaatc gtcttcgcg 
      actcaacct gaaccaaag caagacaaa tttgacacc 
      gagctcacc gatggttga ttcattccc ggctatcaa 
      taataattc acgaactgt ggaaattca agggacgac 
      aaacaacaa cagggagaa taatggctt tcctatgac 
      accaacaga agctcttaa actgtatgg gaaccgttt 
      gacagagta cgagaagaa tgaaataga gaagtattc 
      caagatttg gtatttggg cttaaatgc ttgtaaaat 
      tcatggcga agttggggg ttcgcaaaa ctgtggtta 
      cgacgacga aagtgggag ctacatcaa ggttctgca 
      cgatcacat ctgctaccg atacgaaat cctggaagt 
      tattggaaa gggtagctt tgggcaagt catcagggc 
      gctcgatca catgaccaa taagcatgt agcgatcaa 
      aatcataag gaacaagaa gcggttcca acatccggc 
      actggtaga agtccgaat actggatca tttgcgaaa 
      aaaagacaa agatggaaa tcacaacgt catccacat 
      gtctgaaca tttcacctt tagaaatca cctctgcat 
      aagtttcga gttactaag ccttaatct tttcgaact 
      gctgaagaa aaacagcta ccgtggatt tagtctaaa 
      tttaatcag aaggtttgc cagttccat tctcaaatg 
      tctgaggct tctccagaa ggaaaacat catacactg 
      cgatcttaa accggaaaa tgttctgct gaaggaaag 
      gggaagtac ttcgataag agtaataga tttcggaag 
      ttcgtgtta cacaaacca acgggttta cacttacat 
      ccagtctcg tttttatcg atcaccgga agtcatatt 
      ggggttgcc ctatgggac gcctattga tatgtggag 
      ttttggctg tatcttggc cgagttgta cacgggtta 
      tccattatt tcctggcga aaatgaagt cgagcaact 
      tgcgtgctt gatggaagt tttgggaca gccacctga 
      cgagctcat tgtcaacgc tactagaaa gagattatt 
      tttcgattc ccgggggaa ccccagatg catcacaaa 
      ctccaaggg caagaaacg caagccagg atcgaagaa 
      cttgtcgac agcactgca ctgtgatga tcccttgtt 
      tatggactt catatccag gtgtcttga gtggaacgc 
      caagaagag aatgacacc agatgaagc gttgcgcca 
      cgaatggat tctctcggg cacttcggg ctaccaaaa 
      gtctggaaa tgacttacg acacagcca ttctgaagt 
      aaacgtagg ccacgagag ttattcgtc tagcagtac 
      ttccggcag cagccattc ccgcattta tcataaaaa 
      tatcgctcc caagggtga taaagtcaa ggccaaaat 
      tttagatac tagattaca tgatagtat ttctaaact 
      agattcaaa cttgaacga ttcgggaac ttttcttcc 
      cccgattct ttagtcaag ttgtcgaaa actcaatgg 
      gccaacgat ctccaataa gaccaagtt tttcggtag 
      aagttagta aactagata atcgtattt gttaccaga 
      ttggaagtc ataacagtt tcagtaatt tgattagat 
      aacagggtg caaataatt gttgaaaca ggcgaaaaa 
      tactacagc catttcgtt actgagaga agtggttat 
      tgaaactgc tatctacaa gagtagatt tttagtaca 
      atcataaac gtaatgaat atagaagta tttctccta 
      agcaaatct gttatcaaa atgctataa agttgttgt 
      gactgatac atattgcct aaatgtata ataaaattt 
      tagatatac agaattgaa tagaatgtg acataaata 
      ttaatcgtt gaaagcttc atatgttcc ttgaatagg 
      tatattatc ttttgatat tcaagtttg tttgctaac 
      tctctgtta ctactctct actaataaa gcttccagt 
      ttattgtaa cggagattg gcatacccc acaaaccac 
      gtaaataat tcatttttg taagtttaa ttaaggtgt 
      ctaattatt actaataac taatttaag tttgtacct 
      tcgtttcta agtttttta tttctttct gttaaaaaa 
      taataatgc caacatatg ttttattta acgttatat 
      ttagaagtg ttgtactaa tgtaattta atgttctat 
      acaacaaat aagcgtgac ctatgtact cttggtcac 
      atatctgac atttactgt aaattcccc aatgtgata 
      tatactgat aatattatt atgtataaa attaatata 
      gcagcctaa ctagtagat tcctgacta gtcgaccat 
      ttacaaggt atttcataa tcaaagaaa attttttcg 
      gttgaccac gaaatgtgc actttgata atttcataa 
      tgaaattga aattttaca caaaacact ttataaaat 
      attcagttc gcttaaaat ataaaagtg taacataa
    • >TC014135 RB
      tgctttgac agatcttca atccagaca aggcaccgt 
      acagctcga ataccatca gtggtcttc cccacacat 
      acctacgcc aaagagtga atgatccga tatcatcgt 
      gtctggagg atcatcaca ctcacacga tcgcactcg 
      catttctcg atagtcggt gtcggaaag tgcgcgtgt 
      tgactgggg actagcgcg cactgtcgt caactttcc 
      cattgttcg ttagctctt tgaattttg aaaattttt 
      ggtcagctg agtgataca tgtgaacat tttgccaaa 
      atgctcatg ttcgagcgg taatcgctg aataatatg 
      aaaagaagc agatcggtg atggaggag acgaccatg 
      cgcgatctc cgcgtattg atcgtcggg aacgcgggc 
      cacagcatc gctcaggca atggcatta gtttgcgac 
      ctcatctca atagggtcc ggtgccgcc tcctggtgc 
      gtagtgagt gcgtgtcat gggctcgga aaccccagc 
      ggcccgcgg cccttgatg tgtgcctac cacacgtac 
      cccagcacc cgggggtcc cgaactatg ggctggact 
      cacgctgac cactcgagt ccagcagct caccaccat 
      aaagaagta ttaccgaca aagtatggg tccaagcca 
      ccgacgact gcatccggc aagcacttg aataaatct 
      tcttccaaa aatggtcat tcaacatcg aaaactacg 
      cttatttcc gaattgccg ttattggaa agcagcaaa 
      tcgtcttcg cgactcaac ctgaaccaa agcaagaca 
      aatttgaca ccgagctca ccgatggtt gattcattc 
      ccggctatc aataataat tcacgaact gtggaaatt 
      caagggacg acaaacaac aacagggag aataatggc 
      tttcctatg acaccaaca gaagctctt aaactgtat 
      gggaaccgt ttgacagag tacgagaag aatgaaata 
      gagaagtat tccaagatt tggtatttg ggcttaaat 
      gcttgtaaa attcatggc gaagttggg ggttcgcaa 
      aactgtggt tacgacgac gaaagtggg agctacatc 
      aaggttctg cacgatcac atctgctac cgatacgaa 
      atcctggaa gttattgga aagggtagc tttgggcaa 
      gtcatcagg gcgctcgat cacatgacc aataagcat 
      gtagcgatc aaaatcata aggaacaag aagcggttc 
      caacatccg gcactggta gaagtccga atactggat 
      catttgcga aaaaaagac aaagatgga aatcacaac 
      gtcatccac atgtctgaa catttcacc tttagaaat 
      cacctctgc ataagtttc gagttacta agccttaat 
      cttttcgaa ctgctgaag aaaaacagc taccgtgga 
      tttagtcta aatttaatc agaaggttt gccagttcc 
      attctcaaa tgtctgagg cttctccag aaggaaaac 
      atcatacac tgcgatctt aaaccggaa aatgttctg 
      ctgaaggaa aggggaagt acttcgata agagtaata 
      gatttcgga agttcgtgt tacacaaac caacgggtt 
      tacacttac atccagtct cgtttttat cgatcaccg 
      gaagtcata ttggggttg ccctatggg acgcctatt 
      gatatgtgg agttttggc tgtatcttg gccgagttg 
      tacacgggt tatccatta tttcctggc gaaaatgaa 
      gtcgagcaa cttgcgtgc ttgatggaa gttttggga 
      cagccacct gacgagctc attgtcaac gctactaga 
      aagagatta tttttcgat tcccggggg aaccccaga 
      tgcatcaca aactccaag ggcaagaaa cgcaagcca 
      ggatcgaag aacttgtcg acagcactg cactgtgat 
      gatcccttg tttatggac ttcatatcc aggtgtctt 
      gagtggaac gccaagaag agaatgaca ccagatgaa 
      gcgttgcgc cacgaatgg attctctcg ggcacttcg 
      ggctaccaa aagtctgga aatgactta cgacacagc 
      cattctgaa gtaaacgta ggccacgag agttattcg 
      tctagcagt acttccggc agcagccat tcccgcatt 
      tatcataaa aatatcgct cccaagggt gataaagtc 
      aaggccaaa attttagat actagatta catgatagt 
      atttctaaa ctagattca aacttgaac gattcggga 
      acttttctt cccccgatt ctttagtca agttgtcga 
      aaactcaat gggccaacg atctccaat aagaccaag 
      tttttcggt agaagttag taaactaga taatcgtat 
      ttgttacca gattggaag tcataacag tttcagtaa 
      tttgattag ataacaggg tgcaaataa ttgttgaaa 
      caggcgaaa aatactaca gccatttcg ttactgaga 
      gaagtggtt attgaaact gctatctac aagagtaga 
      tttttagta caatcataa acgtaatga atatagaag 
      tatttctcc taagcaaat ctgttatca aaatgctat 
      aaagttgtt gtgactgat acatattgc ctaaatgta 
      taataaaat tttagatat acagaattg aatagaatg 
      tgacataaa tattaatcg ttgaaagct tcatatgtt 
      ccttgaata ggtatatta tcttttgat attcaagtt 
      tgtttgcta actctctgt tactactct ctactaata 
      aagcttcca gtttattgt aacggagat tggcatacc 
      ccacaaacc acgtaaata attcatttt tgtaagttt 
      aattaaggt gtctaatta ttactaata actaattta 
      agtttgtac cttcgtttc taagttttt tatttcttt 
      ctgttaaaa aataataat gccaacata tgttttatt 
      taacgttat atttagaag tgttgtact aatgtaatt 
      taatgttct atacaacaa ataagcgtg acctatgta 
      ctcttggtc acatatctg acatttact gtaaattcc 
      ccaatgtga tatatactg ataatatta ttatgtata 
      aaattaata tagcagcct aactagtag attcctgac 
      tagtcgacc atttacaag gtatttcat aatcaaaga 
      aaatttttt cggttgacc acgaaatgt gcactttga 
      taatttcat aatgaaatt gaaatttta cacaaaaca 
      ctttataaa atattcagt tcgcttaaa atataaaag 
      tgtaacata a
    • >TC014135 RC
      tgctttgac agatcttca atccagaca aggcaccgt 
      acagctcga ataccatca gtggtcttc cccacacat 
      acctacgcc aaagagtga atgatccga tatcatcgt 
      gtctggagg atcatcaca ctcacacga tcgcactcg 
      catttctcg atagtcggt gtcggaaag tgcgcgtgt 
      tgactgggg actagcgcg cactgtcgt caactttcc 
      cattgttcg ttagctctt tgaattttg aaaattttt 
      ggtcagctg agtgataca tgtgaacat tttgccaaa 
      atgctcatg ttcgagcgg taatcgctg aataatatg 
      aaaagaagc agatcggtg atggaggag acgaccatg 
      cgcgatctc cgcgtattg atcgtcggg aacgcgggc 
      cacagcatc gctcaggca atggcatta gtttgcgac 
      ctcatctca atagggtcc ggtgccgcc tcctggtgc 
      gtagtgagt gcgtgtcat gggctcgga aaccccagc 
      ggcccgcgg cccttgatg tgtgcctac cacacgtac 
      cccagcacc cgggggtcc cgaactatg ggctggact 
      cacgctgac cactcggta ttccataca attagtcca 
      gcagctcac caccataaa gaagtatta ccgacaaag 
      tatgggtcc aagccaccg acgactgca tccggcaag 
      cacttgaat aaatcttct tccaaaaat ggtcattca 
      acatcgaaa actacgctt atttccgaa ttgccgtta 
      ttggaaagc agcaaatcg tcttcgcga ctcaacctg 
      aaccaaagc aagacaaat ttgacaccg agctcaccg 
      atggttgat tcattcccg gctatcaat aataattca 
      cgaactgtg gaaattcaa gggacgaca aacaacaac 
      agggagaat aatggcttt cctatgaca ccaacagaa 
      gctcttaaa ctgtatggg aaccgtttg acagagtac 
      gagaagaat gaaatagag aagtattcc aagatttgg 
      tatttgggc ttaaatgct tgtaaaatt catggcgaa 
      gttgggggt tcgcaaaac tgtggttac gacgacgaa 
      agtgggagc tacatcaag gttctgcac gatcacatc 
      tgctaccga tacgaaatc ctggaagtt attggaaag 
      ggtagcttt gggcaagtc atcagggcg ctcgatcac 
      atgaccaat aagcatgta gcgatcaaa atcataagg 
      aacaagaag cggttccaa catccggca ctggtagaa 
      gtccgaata ctggatcat ttgcgaaaa aaagacaaa 
      gatggaaat cacaacgtc atccacatg tctgaacat 
      ttcaccttt agaaatcac ctctgcata agtttcgag 
      ttactaagc cttaatctt ttcgaactg ctgaagaaa 
      aacagctac cgtggattt agtctaaat ttaatcaga 
      aggtttgcc agttccatt ctcaaatgt ctgaggctt 
      ctccagaag gaaaacatc atacactgc gatcttaaa 
      ccggaaaat gttctgctg aaggaaagg ggaagtact 
      tcgataaga gtaatagat ttcggaagt tcgtgttac 
      acaaaccaa cgggtttac acttacatc cagtctcgt 
      ttttatcga tcaccggaa gtcatattg gggttgccc 
      tatgggacg cctattgat atgtggagt tttggctgt 
      atcttggcc gagttgtac acgggttat ccattattt 
      cctggcgaa aatgaagtc gagcaactt gcgtgcttg 
      atggaagtt ttgggacag ccacctgac gagctcatt 
      gtcaacgct actagaaag agattattt ttcgattcc 
      cgggggaac cccagatgc atcacaaac tccaagggc 
      aagaaacgc aagccagga tcgaagaac ttgtcgaca 
      gcactgcac tgtgatgat cccttgttt atggacttc 
      atatccagg tgtcttgag tggaacgcc aagaagaga 
      atgacacca gatgaagcg ttgcgccac gaatggatt 
      ctctcgggc acttcgggc taccaaaag tctggaaat 
      gacttacga cacagccat tctgaagta aacgtaggc 
      cacgagagt tattcgtct agcagtact tccggcagc 
      agccattcc cgcatttat cataaaaat atcgctccc 
      aagggtgat aaagtcaag gccaaaatt ttagatact 
      agattacat gatagtatt tctaaacta gattcaaac 
      ttgaacgat tcgggaact tttcttccc ccgattctt 
      tagtcaagt tgtcgaaaa ctcaatggg ccaacgatc 
      tccaataag accaagttt ttcggtaga agttagtaa 
      actagataa tcgtatttg ttaccagat tggaagtca 
      taacagttt cagtaattt gattagata acagggtgc 
      aaataattg ttgaaacag gcgaaaaat actacagcc 
      atttcgtta ctgagagaa gtggttatt gaaactgct 
      atctacaag agtagattt ttagtacaa tcataaacg 
      taatgaata tagaagtat ttctcctaa gcaaatctg 
      ttatcaaaa tgctataaa gttgttgtg actgataca 
      tattgccta aatgtataa taaaatttt agatataca 
      gaattgaat agaatgtga cataaatat taatcgttg 
      aaagcttca tatgttcct tgaataggt atattatct 
      tttgatatt caagtttgt ttgctaact ctctgttac 
      tactctcta ctaataaag cttccagtt tattgtaac 
      ggagattgg catacccca caaaccacg taaataatt 
      catttttgt aagtttaat taaggtgtc taattatta 
      ctaataact aatttaagt ttgtacctt cgtttctaa 
      gttttttat ttctttctg ttaaaaaat aataatgcc 
      aacatatgt tttatttaa cgttatatt tagaagtgt 
      tgtactaat gtaatttaa tgttctata caacaaata 
      agcgtgacc tatgtactc ttggtcaca tatctgaca 
      tttactgta aattcccca atgtgatat atactgata 
      atattatta tgtataaaa ttaatatag cagcctaac 
      tagtagatt cctgactag tcgaccatt tacaaggta 
      tttcataat caaagaaaa ttttttcgg ttgaccacg 
      aaatgtgca ctttgataa tttcataat gaaattgaa 
      attttacac aaaacactt tataaaata ttcagttcg 
      cttaaaata taaaagtgt aacataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY