General information | |||
TC014131 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 6405431..6415224 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC014131 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05564 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC014131 RA
MISEIENEE IRAIPQEKY NLVVWTPHE KLFRNRVTL RNTVLSEIA VPFTIALQV KLRRSVFGT YLDDFNENQ CQNEFLNTN KLSIMESVM RDFNSRQEN LFDYNNNLF DHVIRYLNN TVTETKQID CDFLYEHAT HLRMDDYHI NMVDDGVRN FRNLQVLVI CGNWIQDID GSVLPRRLK FLELYVNEI ESLDNLTHK PPKHLLHLG LARNKLTND SHLHLLAAC RRFNNLKSL DLSECDIYR LVDVLHELK PLQNLEGLL LQGNPCSKK VKQTIHIEF KMPLFQLNI KEDLDKESE KDLVRNKSK ERKKGKKGH KDKKSKSGK STKKGSKKG GKSPYERPN EYNPKKINN AFQSQRKAW GNNLKFTPL VLQSPENNL LTYRDTFRS CVNVSVIYL QYKPPEKKK KKKKGSKKD KKSKTSSKK SEKSSKSKK GKKKSDKSS STKSKSKEK IKEEKQSIE PKETILKRV TIANFDCNL FTASWKNNI HFFWSEEPS FGRNAVELN SYRENLRKK AEEEKRLKQ IKEEEAKRK KQKSDKKKG KDKKSAKSG KSSKSTKSK KSKKSKKSK KSKKSKSSK KSKAEKFLT CEVSFGLAR LGETLRETR SGSDVKKTK KRKKGKK
- >TC014131 RA
atgatatcc gaaattgaa aatgaggaa atcagagcg attccgcag gaaaaatat aatttggtt gtttggacg ccgcacgaa aaattattt cgcaacaga gttacactg aggaatacg gttttgtct gaaattgct gtgcctttt acgatcgct ctgcaggtg aaattgcgc cgatcagta ttcggaact tacctggat gatttcaat gaaaatcaa tgccaaaac gaatttctc aatacaaat aaactgagc attatggag agcgtcatg agggatttt aattcgaga caagagaac ctgtttgat tacaataat aacttattc gaccatgta ataagatac ttgaataat actgtcaca gaaacgaag caaattgac tgtgacttt ctttacgag catgcgaca cacctacga atggatgat tatcatata aacatggta gatgatggc gtaagaaac ttccgaaat cttcaggta ctggttatc tgcggtaac tggattcaa gatatcgac ggttcggta ctccctaga cgcttaaaa tttctggaa ctttacgtg aacgaaatt gaatcatta gataattta acgcacaag ccccccaaa catttgtta cacttgggg ttagcgcga aacaaatta acaaatgac agtcaccta catctgttg gctgcttgt cgacgcttt aacaatttg aaatcttta gatttatcg gagtgtgac atctaccgt ttggtggat gtattacac gaactgaaa ccgcttcaa aatttggaa gggttgctt ctacaggga aatccatgt tcgaaaaaa gttaagcaa acaattcat attgagttt aaaatgcct ttgtttcaa ttaaatatt aaagaagat ttagataaa gaaagtgaa aaagatcta gtacgcaat aaatctaaa gaaagaaaa aaaggaaaa aagggacat aaagacaag aaaagtaaa tctgggaaa agtacaaaa aaaggttcc aagaaaggt gggaaatct ccttacgaa cgaccaaat gaatataac ccgaaaaag ataaataac gcatttcag tctcaaagg aaagcgtgg ggaaataat ttgaaattt acgccactt gtgctgcaa tcacctgaa aataactta cttacctat agagatact tttcgaagt tgtgttaac gtctcagta atttatttg cagtacaaa ccgccagaa aagaagaaa aagaagaag aaagggtcc aaaaaggac aagaaaagt aaaacaagt agtaaaaaa tctgaaaaa tccagtaaa agtaaaaaa ggtaaaaaa aaatctgac aagtctagt agcacaaaa agtaaaagc aaagaaaaa attaaagaa gaaaaacaa tcaatagaa ccgaaagaa acaatcttg aagcgagtg actatagct aattttgac tgtaactta tttacggct agttggaaa aataatatt cactttttt tggtccgaa gaaccctct ttcgggcgt aatgcagtg gagttaaac agttaccga gagaatttg agaaaaaaa gctgaagaa gaaaaaaga ctgaaacag atcaaagaa gaagaagcg aaaagaaaa aaacagaaa agtgataaa aagaagggt aaagataag aaatcggca aaatctggg aaatcaagt aaatcgacg aaatcaaaa aaatccaaa aaaagtaaa aaatccaag aaaagtaaa aaatcgaaa tcttcgaaa aaatcaaaa gctgaaaag tttttgact tgtgaggtt agttttggg ttagccaga ttaggtgaa acgctacga gaaacaaga agtggcagc gatgttaag aaaaccaaa aagaggaaa aaaggaaaa aaataa
- >TC014131 RA
catttctga aacaaaatg atatccgaa attgaaaat gaggaaatc agagcgatt ccgcaggaa aaatataat ttggttgtt tggacgccg cacgaaaaa ttatttcgc aacagagtt acactgagg aatacggtt ttgtctgaa attgctgtg ccttttacg atcgctctg caggtgaaa ttgcgccga tcagtattc ggaacttac ctggatgat ttcaatgaa aatcaatgc caaaacgaa tttctcaat acaaataaa ctgagcatt atggagagc gtcatgagg gattttaat tcgagacaa gagaacctg tttgattac aataataac ttattcgac catgtaata agatacttg aataatact gtcacagaa acgaagcaa attgactgt gactttctt tacgagcat gcgacacac ctacgaatg gatgattat catataaac atggtagat gatggcgta agaaacttc cgaaatctt caggtactg gttatctgc ggtaactgg attcaagat atcgacggt tcggtactc cctagacgc ttaaaattt ctggaactt tacgtgaac gaaattgaa tcattagat aatttaacg cacaagccc cccaaacat ttgttacac ttggggtta gcgcgaaac aaattaaca aatgacagt cacctacat ctgttggct gcttgtcga cgctttaac aatttgaaa tctttagat ttatcggag tgtgacatc taccgtttg gtggatgta ttacacgaa ctgaaaccg cttcaaaat ttggaaggg ttgcttcta cagggaaat ccatgttcg aaaaaagtt aagcaaaca attcatatt gagtttaaa atgcctttg tttcaatta aatattaaa gaagattta gataaagaa agtgaaaaa gatctagta cgcaataaa tctaaagaa agaaaaaaa ggaaaaaag ggacataaa gacaagaaa agtaaatct gggaaaagt acaaaaaaa ggttccaag aaaggtggg aaatctcct tacgaacga ccaaatgaa tataacccg aaaaagata aataacgca tttcagtct caaaggaaa gcgtgggga aataatttg aaatttacg ccacttgtg ctgcaatca cctgaaaat aacttactt acctataga gatactttt cgaagttgt gttaacgtc tcagtaatt tatttgcag tacaaaccg ccagaaaag aagaaaaag aagaagaaa gggtccaaa aaggacaag aaaagtaaa acaagtagt aaaaaatct gaaaaatcc agtaaaagt aaaaaaggt aaaaaaaaa tctgacaag tctagtagc acaaaaagt aaaagcaaa gaaaaaatt aaagaagaa aaacaatca atagaaccg aaagaaaca atcttgaag cgagtgact atagctaat tttgactgt aacttattt acggctagt tggaaaaat aatattcac tttttttgg tccgaagaa ccctctttc gggcgtaat gcagtggag ttaaacagt taccgagag aatttgaga aaaaaagct gaagaagaa aaaagactg aaacagatc aaagaagaa gaagcgaaa agaaaaaaa cagaaaagt gataaaaag aagggtaaa gataagaaa tcggcaaaa tctgggaaa tcaagtaaa tcgacgaaa tcaaaaaaa tccaaaaaa agtaaaaaa tccaagaaa agtaaaaaa tcgaaatct tcgaaaaaa tcaaaagct gaaaagttt ttgacttgt gaggttagt tttgggtta gccagatta ggtgaaacg ctacgagaa acaagaagt ggcagcgat gttaagaaa accaaaaag aggaaaaaa ggaaaaaaa taaactata gcaaatgat cttatattt ttgtatgac aattgtaat ataaagtcc acatataa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
