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Gene information
General information
TC014131 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6405431..6415224
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC014131
      iBeetle screen iB_05564

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014131 RA
        MISEIENEE IRAIPQEKY NLVVWTPHE KLFRNRVTL 
RNTVLSEIA VPFTIALQV KLRRSVFGT YLDDFNENQ 
CQNEFLNTN KLSIMESVM RDFNSRQEN LFDYNNNLF 
DHVIRYLNN TVTETKQID CDFLYEHAT HLRMDDYHI 
NMVDDGVRN FRNLQVLVI CGNWIQDID GSVLPRRLK 
FLELYVNEI ESLDNLTHK PPKHLLHLG LARNKLTND 
SHLHLLAAC RRFNNLKSL DLSECDIYR LVDVLHELK 
PLQNLEGLL LQGNPCSKK VKQTIHIEF KMPLFQLNI 
KEDLDKESE KDLVRNKSK ERKKGKKGH KDKKSKSGK 
STKKGSKKG GKSPYERPN EYNPKKINN AFQSQRKAW 
GNNLKFTPL VLQSPENNL LTYRDTFRS CVNVSVIYL 
QYKPPEKKK KKKKGSKKD KKSKTSSKK SEKSSKSKK 
GKKKSDKSS STKSKSKEK IKEEKQSIE PKETILKRV 
TIANFDCNL FTASWKNNI HFFWSEEPS FGRNAVELN 
SYRENLRKK AEEEKRLKQ IKEEEAKRK KQKSDKKKG 
KDKKSAKSG KSSKSTKSK KSKKSKKSK KSKKSKSSK 
KSKAEKFLT CEVSFGLAR LGETLRETR SGSDVKKTK 
KRKKGKK
      CDS sequence
      • >TC014131 RA
        atgatatcc gaaattgaa aatgaggaa atcagagcg 
attccgcag gaaaaatat aatttggtt gtttggacg 
ccgcacgaa aaattattt cgcaacaga gttacactg 
aggaatacg gttttgtct gaaattgct gtgcctttt 
acgatcgct ctgcaggtg aaattgcgc cgatcagta 
ttcggaact tacctggat gatttcaat gaaaatcaa 
tgccaaaac gaatttctc aatacaaat aaactgagc 
attatggag agcgtcatg agggatttt aattcgaga 
caagagaac ctgtttgat tacaataat aacttattc 
gaccatgta ataagatac ttgaataat actgtcaca 
gaaacgaag caaattgac tgtgacttt ctttacgag 
catgcgaca cacctacga atggatgat tatcatata 
aacatggta gatgatggc gtaagaaac ttccgaaat 
cttcaggta ctggttatc tgcggtaac tggattcaa 
gatatcgac ggttcggta ctccctaga cgcttaaaa 
tttctggaa ctttacgtg aacgaaatt gaatcatta 
gataattta acgcacaag ccccccaaa catttgtta 
cacttgggg ttagcgcga aacaaatta acaaatgac 
agtcaccta catctgttg gctgcttgt cgacgcttt 
aacaatttg aaatcttta gatttatcg gagtgtgac 
atctaccgt ttggtggat gtattacac gaactgaaa 
ccgcttcaa aatttggaa gggttgctt ctacaggga 
aatccatgt tcgaaaaaa gttaagcaa acaattcat 
attgagttt aaaatgcct ttgtttcaa ttaaatatt 
aaagaagat ttagataaa gaaagtgaa aaagatcta 
gtacgcaat aaatctaaa gaaagaaaa aaaggaaaa 
aagggacat aaagacaag aaaagtaaa tctgggaaa 
agtacaaaa aaaggttcc aagaaaggt gggaaatct 
ccttacgaa cgaccaaat gaatataac ccgaaaaag 
ataaataac gcatttcag tctcaaagg aaagcgtgg 
ggaaataat ttgaaattt acgccactt gtgctgcaa 
tcacctgaa aataactta cttacctat agagatact 
tttcgaagt tgtgttaac gtctcagta atttatttg 
cagtacaaa ccgccagaa aagaagaaa aagaagaag 
aaagggtcc aaaaaggac aagaaaagt aaaacaagt 
agtaaaaaa tctgaaaaa tccagtaaa agtaaaaaa 
ggtaaaaaa aaatctgac aagtctagt agcacaaaa 
agtaaaagc aaagaaaaa attaaagaa gaaaaacaa 
tcaatagaa ccgaaagaa acaatcttg aagcgagtg 
actatagct aattttgac tgtaactta tttacggct 
agttggaaa aataatatt cactttttt tggtccgaa 
gaaccctct ttcgggcgt aatgcagtg gagttaaac 
agttaccga gagaatttg agaaaaaaa gctgaagaa 
gaaaaaaga ctgaaacag atcaaagaa gaagaagcg 
aaaagaaaa aaacagaaa agtgataaa aagaagggt 
aaagataag aaatcggca aaatctggg aaatcaagt 
aaatcgacg aaatcaaaa aaatccaaa aaaagtaaa 
aaatccaag aaaagtaaa aaatcgaaa tcttcgaaa 
aaatcaaaa gctgaaaag tttttgact tgtgaggtt 
agttttggg ttagccaga ttaggtgaa acgctacga 
gaaacaaga agtggcagc gatgttaag aaaaccaaa 
aagaggaaa aaaggaaaa aaataa
      mRNA sequence
      • >TC014131 RA
        catttctga aacaaaatg atatccgaa attgaaaat 
gaggaaatc agagcgatt ccgcaggaa aaatataat 
ttggttgtt tggacgccg cacgaaaaa ttatttcgc 
aacagagtt acactgagg aatacggtt ttgtctgaa 
attgctgtg ccttttacg atcgctctg caggtgaaa 
ttgcgccga tcagtattc ggaacttac ctggatgat 
ttcaatgaa aatcaatgc caaaacgaa tttctcaat 
acaaataaa ctgagcatt atggagagc gtcatgagg 
gattttaat tcgagacaa gagaacctg tttgattac 
aataataac ttattcgac catgtaata agatacttg 
aataatact gtcacagaa acgaagcaa attgactgt 
gactttctt tacgagcat gcgacacac ctacgaatg 
gatgattat catataaac atggtagat gatggcgta 
agaaacttc cgaaatctt caggtactg gttatctgc 
ggtaactgg attcaagat atcgacggt tcggtactc 
cctagacgc ttaaaattt ctggaactt tacgtgaac 
gaaattgaa tcattagat aatttaacg cacaagccc 
cccaaacat ttgttacac ttggggtta gcgcgaaac 
aaattaaca aatgacagt cacctacat ctgttggct 
gcttgtcga cgctttaac aatttgaaa tctttagat 
ttatcggag tgtgacatc taccgtttg gtggatgta 
ttacacgaa ctgaaaccg cttcaaaat ttggaaggg 
ttgcttcta cagggaaat ccatgttcg aaaaaagtt 
aagcaaaca attcatatt gagtttaaa atgcctttg 
tttcaatta aatattaaa gaagattta gataaagaa 
agtgaaaaa gatctagta cgcaataaa tctaaagaa 
agaaaaaaa ggaaaaaag ggacataaa gacaagaaa 
agtaaatct gggaaaagt acaaaaaaa ggttccaag 
aaaggtggg aaatctcct tacgaacga ccaaatgaa 
tataacccg aaaaagata aataacgca tttcagtct 
caaaggaaa gcgtgggga aataatttg aaatttacg 
ccacttgtg ctgcaatca cctgaaaat aacttactt 
acctataga gatactttt cgaagttgt gttaacgtc 
tcagtaatt tatttgcag tacaaaccg ccagaaaag 
aagaaaaag aagaagaaa gggtccaaa aaggacaag 
aaaagtaaa acaagtagt aaaaaatct gaaaaatcc 
agtaaaagt aaaaaaggt aaaaaaaaa tctgacaag 
tctagtagc acaaaaagt aaaagcaaa gaaaaaatt 
aaagaagaa aaacaatca atagaaccg aaagaaaca 
atcttgaag cgagtgact atagctaat tttgactgt 
aacttattt acggctagt tggaaaaat aatattcac 
tttttttgg tccgaagaa ccctctttc gggcgtaat 
gcagtggag ttaaacagt taccgagag aatttgaga 
aaaaaagct gaagaagaa aaaagactg aaacagatc 
aaagaagaa gaagcgaaa agaaaaaaa cagaaaagt 
gataaaaag aagggtaaa gataagaaa tcggcaaaa 
tctgggaaa tcaagtaaa tcgacgaaa tcaaaaaaa 
tccaaaaaa agtaaaaaa tccaagaaa agtaaaaaa 
tcgaaatct tcgaaaaaa tcaaaagct gaaaagttt 
ttgacttgt gaggttagt tttgggtta gccagatta 
ggtgaaacg ctacgagaa acaagaagt ggcagcgat 
gttaagaaa accaaaaag aggaaaaaa ggaaaaaaa 
taaactata gcaaatgat cttatattt ttgtatgac 
aattgtaat ataaagtcc acatataa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY