Status
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Gene information
General information
TC014131 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6405431..6415224
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC014131
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_05564

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC014131 RA
        MISEIENEE IRAIPQEKY NLVVWTPHE KLFRNRVTL 
        RNTVLSEIA VPFTIALQV KLRRSVFGT YLDDFNENQ 
        CQNEFLNTN KLSIMESVM RDFNSRQEN LFDYNNNLF 
        DHVIRYLNN TVTETKQID CDFLYEHAT HLRMDDYHI 
        NMVDDGVRN FRNLQVLVI CGNWIQDID GSVLPRRLK 
        FLELYVNEI ESLDNLTHK PPKHLLHLG LARNKLTND 
        SHLHLLAAC RRFNNLKSL DLSECDIYR LVDVLHELK 
        PLQNLEGLL LQGNPCSKK VKQTIHIEF KMPLFQLNI 
        KEDLDKESE KDLVRNKSK ERKKGKKGH KDKKSKSGK 
        STKKGSKKG GKSPYERPN EYNPKKINN AFQSQRKAW 
        GNNLKFTPL VLQSPENNL LTYRDTFRS CVNVSVIYL 
        QYKPPEKKK KKKKGSKKD KKSKTSSKK SEKSSKSKK 
        GKKKSDKSS STKSKSKEK IKEEKQSIE PKETILKRV 
        TIANFDCNL FTASWKNNI HFFWSEEPS FGRNAVELN 
        SYRENLRKK AEEEKRLKQ IKEEEAKRK KQKSDKKKG 
        KDKKSAKSG KSSKSTKSK KSKKSKKSK KSKKSKSSK 
        KSKAEKFLT CEVSFGLAR LGETLRETR SGSDVKKTK 
        KRKKGKK
      CDS sequence
      • >TC014131 RA
        atgatatcc gaaattgaa aatgaggaa atcagagcg 
        attccgcag gaaaaatat aatttggtt gtttggacg 
        ccgcacgaa aaattattt cgcaacaga gttacactg 
        aggaatacg gttttgtct gaaattgct gtgcctttt 
        acgatcgct ctgcaggtg aaattgcgc cgatcagta 
        ttcggaact tacctggat gatttcaat gaaaatcaa 
        tgccaaaac gaatttctc aatacaaat aaactgagc 
        attatggag agcgtcatg agggatttt aattcgaga 
        caagagaac ctgtttgat tacaataat aacttattc 
        gaccatgta ataagatac ttgaataat actgtcaca 
        gaaacgaag caaattgac tgtgacttt ctttacgag 
        catgcgaca cacctacga atggatgat tatcatata 
        aacatggta gatgatggc gtaagaaac ttccgaaat 
        cttcaggta ctggttatc tgcggtaac tggattcaa 
        gatatcgac ggttcggta ctccctaga cgcttaaaa 
        tttctggaa ctttacgtg aacgaaatt gaatcatta 
        gataattta acgcacaag ccccccaaa catttgtta 
        cacttgggg ttagcgcga aacaaatta acaaatgac 
        agtcaccta catctgttg gctgcttgt cgacgcttt 
        aacaatttg aaatcttta gatttatcg gagtgtgac 
        atctaccgt ttggtggat gtattacac gaactgaaa 
        ccgcttcaa aatttggaa gggttgctt ctacaggga 
        aatccatgt tcgaaaaaa gttaagcaa acaattcat 
        attgagttt aaaatgcct ttgtttcaa ttaaatatt 
        aaagaagat ttagataaa gaaagtgaa aaagatcta 
        gtacgcaat aaatctaaa gaaagaaaa aaaggaaaa 
        aagggacat aaagacaag aaaagtaaa tctgggaaa 
        agtacaaaa aaaggttcc aagaaaggt gggaaatct 
        ccttacgaa cgaccaaat gaatataac ccgaaaaag 
        ataaataac gcatttcag tctcaaagg aaagcgtgg 
        ggaaataat ttgaaattt acgccactt gtgctgcaa 
        tcacctgaa aataactta cttacctat agagatact 
        tttcgaagt tgtgttaac gtctcagta atttatttg 
        cagtacaaa ccgccagaa aagaagaaa aagaagaag 
        aaagggtcc aaaaaggac aagaaaagt aaaacaagt 
        agtaaaaaa tctgaaaaa tccagtaaa agtaaaaaa 
        ggtaaaaaa aaatctgac aagtctagt agcacaaaa 
        agtaaaagc aaagaaaaa attaaagaa gaaaaacaa 
        tcaatagaa ccgaaagaa acaatcttg aagcgagtg 
        actatagct aattttgac tgtaactta tttacggct 
        agttggaaa aataatatt cactttttt tggtccgaa 
        gaaccctct ttcgggcgt aatgcagtg gagttaaac 
        agttaccga gagaatttg agaaaaaaa gctgaagaa 
        gaaaaaaga ctgaaacag atcaaagaa gaagaagcg 
        aaaagaaaa aaacagaaa agtgataaa aagaagggt 
        aaagataag aaatcggca aaatctggg aaatcaagt 
        aaatcgacg aaatcaaaa aaatccaaa aaaagtaaa 
        aaatccaag aaaagtaaa aaatcgaaa tcttcgaaa 
        aaatcaaaa gctgaaaag tttttgact tgtgaggtt 
        agttttggg ttagccaga ttaggtgaa acgctacga 
        gaaacaaga agtggcagc gatgttaag aaaaccaaa 
        aagaggaaa aaaggaaaa aaataa
      mRNA sequence
      • >TC014131 RA
        catttctga aacaaaatg atatccgaa attgaaaat 
        gaggaaatc agagcgatt ccgcaggaa aaatataat 
        ttggttgtt tggacgccg cacgaaaaa ttatttcgc 
        aacagagtt acactgagg aatacggtt ttgtctgaa 
        attgctgtg ccttttacg atcgctctg caggtgaaa 
        ttgcgccga tcagtattc ggaacttac ctggatgat 
        ttcaatgaa aatcaatgc caaaacgaa tttctcaat 
        acaaataaa ctgagcatt atggagagc gtcatgagg 
        gattttaat tcgagacaa gagaacctg tttgattac 
        aataataac ttattcgac catgtaata agatacttg 
        aataatact gtcacagaa acgaagcaa attgactgt 
        gactttctt tacgagcat gcgacacac ctacgaatg 
        gatgattat catataaac atggtagat gatggcgta 
        agaaacttc cgaaatctt caggtactg gttatctgc 
        ggtaactgg attcaagat atcgacggt tcggtactc 
        cctagacgc ttaaaattt ctggaactt tacgtgaac 
        gaaattgaa tcattagat aatttaacg cacaagccc 
        cccaaacat ttgttacac ttggggtta gcgcgaaac 
        aaattaaca aatgacagt cacctacat ctgttggct 
        gcttgtcga cgctttaac aatttgaaa tctttagat 
        ttatcggag tgtgacatc taccgtttg gtggatgta 
        ttacacgaa ctgaaaccg cttcaaaat ttggaaggg 
        ttgcttcta cagggaaat ccatgttcg aaaaaagtt 
        aagcaaaca attcatatt gagtttaaa atgcctttg 
        tttcaatta aatattaaa gaagattta gataaagaa 
        agtgaaaaa gatctagta cgcaataaa tctaaagaa 
        agaaaaaaa ggaaaaaag ggacataaa gacaagaaa 
        agtaaatct gggaaaagt acaaaaaaa ggttccaag 
        aaaggtggg aaatctcct tacgaacga ccaaatgaa 
        tataacccg aaaaagata aataacgca tttcagtct 
        caaaggaaa gcgtgggga aataatttg aaatttacg 
        ccacttgtg ctgcaatca cctgaaaat aacttactt 
        acctataga gatactttt cgaagttgt gttaacgtc 
        tcagtaatt tatttgcag tacaaaccg ccagaaaag 
        aagaaaaag aagaagaaa gggtccaaa aaggacaag 
        aaaagtaaa acaagtagt aaaaaatct gaaaaatcc 
        agtaaaagt aaaaaaggt aaaaaaaaa tctgacaag 
        tctagtagc acaaaaagt aaaagcaaa gaaaaaatt 
        aaagaagaa aaacaatca atagaaccg aaagaaaca 
        atcttgaag cgagtgact atagctaat tttgactgt 
        aacttattt acggctagt tggaaaaat aatattcac 
        tttttttgg tccgaagaa ccctctttc gggcgtaat 
        gcagtggag ttaaacagt taccgagag aatttgaga 
        aaaaaagct gaagaagaa aaaagactg aaacagatc 
        aaagaagaa gaagcgaaa agaaaaaaa cagaaaagt 
        gataaaaag aagggtaaa gataagaaa tcggcaaaa 
        tctgggaaa tcaagtaaa tcgacgaaa tcaaaaaaa 
        tccaaaaaa agtaaaaaa tccaagaaa agtaaaaaa 
        tcgaaatct tcgaaaaaa tcaaaagct gaaaagttt 
        ttgacttgt gaggttagt tttgggtta gccagatta 
        ggtgaaacg ctacgagaa acaagaagt ggcagcgat 
        gttaagaaa accaaaaag aggaaaaaa ggaaaaaaa 
        taaactata gcaaatgat cttatattt ttgtatgac 
        aattgtaat ataaagtcc acatataa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY