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Gene information
General information
TC014111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5748671..5754883
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014111
    iBeetle screen iB_05561

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 30

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014111 RA
      MSTNIQVAI RIRPLIASE EAKKLQVQW GTQKNNIFQ 
      IDDNGQKFG DVFSFDHIF GVDKTNTDI YDNIVKDFV 
      ESSLNGLNS TIFAYGQTS SGKTYTMLG DKKHRGIMS 
      LAIENIFEH IENSTDRKF LIRVSYIEI YNEKIYDLL 
      DPSNKEVKI REFFPATIG LQNIKEEIV TSRKQMYEC 
      LRTGTLNRH IAGTKANDR SSRSHTIFK ITIESTQVS 
      DFTSGPVQV SSLNLVDLA GSERVAQTK ATGVRLKEG 
      SHINKSLSA LGLVIRQLS DGQEFINFR DSKLTRLLQ 
      DSLGGNSKT LIIATITLA SIEDTCSTL AFAQRAKAV 
      KNKPHVNEI LTDADLSKR YASLNSQLQ KKLEEQLRI 
      NQKLQETQE VNVREMSRL QQQMELIEH FQRGKISEI 
      PSIPKRRHT IGFATPMPL SSIVFKPDC DTSNQDPHS 
      LLFLDEFSS KDHTALGNT LETIVEEDF ITPMKRNLE 
      KQRRSSYSS VCSEFPVED NDALKKITE LEEEIYNEK 
      QKQQECEIQ LKELNDKFK LREEDLCNK ILELEKKLE 
      NENSDNFYS TIIGFKDQL LYLQQILSN EKIETEFSN 
      SYSEKDLTN KSYSLELSE VKECDELHL ELMDKEQEE 
      RKCLSILEE NEKLKMELI HYETKNKFL TDELDRLTT 
      EIKQKHTLK NSNEMELTL DEYELKQDY IKLKNEFTA 
      SCTENEFLQ KSNEEMKRK IEKYDSLKQ EYIQLKNDL 
      TTSRTENDS LLKLIEEMK GRIEEYHSL NIQLQNKLK 
      MNRKTEEYD SLSQQYIQL KNELTSSST EKEFLLKSN 
      EEMRRRIEE YELLEQQYI QFKNEFTTL CTENDALQN 
      SVEEMKRGI EEYQSLKSE YIQLQDEFN AFCSEKESL 
      KKSNEEMKK RTEEYELLK QQYIQLKNE FNASCTEKD 
      SLQKLNEEI KRIVEENES LKQEYIQLN NKFNASCTE 
      KDSLQKSNE EMKRKIEEN ESLKEEYVQ LKNEFSATC 
      REKESLQKL NEEIKRRIE ESESLKQEY IQLKNKFDA 
      SCTEKDSLQ KSNEEMKRK IEENESLKE EYVQLKNEF 
      STTCREKES LHKLNEEIK RKIEESESL KQEYIQLKN 
      KFNASCTEK DSLLKSNEE MKRKIEENE SLKQEYAQL 
      KNELSASCR EKESLQKLI EESKREVEE SESLKQEHI 
      QLKIKFNDL CAEKDSWQK SNEEMKRKI EENESLKEE 
      YIQLKNEFS ATCREKESL HKLNEKIKR KIEESESLK 
      QEYIEFKNK FTALCTEKD SLLKLEYAQ LKNEFKVSC 
      TEKDSLKKS NEEMRMIIE EQKKKIENM KICNDKLNL 
      EIRNKETNS KEQLCTMSS QNNYLKNEL EKLKNEVAD 
      QKRIISANK EKLHCNDLE LQKQKRRLL EGEEHVKEL 
      EYKIRQSSE KESNFRTII EAQNVTIDK LNLKYRNIL 
      GSIMEYFIS EQKSLLLIR HSQDERENF KSIIRDLKT 
      NNATLKSQV EQLRNENNC DLCAIQETI KKSHLIFKD 
      YFSLEKTFL KAQIIDLEN TNEEYLKEM MALKDQQRN 
      DSTEDCSNC DKLQNEITR LTEVATSQD KLVVTLEKE 
      NSTLRKERF ESVKQVVHL QQQIFDEQD KKSELQYTE 
      WMKFLQDNN KLQQQEIRE LKRKIKKYE NMHSRSVNK 
      QSVGTECNL LGAPATEID KSKNVRVED VQNGCLHFS 
      GKISTIHAE VQCNIFSEP SALQEKYDL LKQIYRKKN 
      NEIQSLNAE INQLKLKVS SL
    CDS sequence
    • >TC014111 RA
      atgtctaca aatatacaa gtagcaata agaatacgt 
      cccctcatc gcgagtgag gaggccaaa aagcttcag 
      gtgcagtgg ggcacccaa aagaacaac attttccaa 
      attgacgac aatgggcaa aagtttgga gacgtcttc 
      agctttgac cacattttc ggagttgat aaaacaaac 
      acggacatt tatgacaac attgtgaag gattttgtg 
      gaatcgtct ttaaacggc ttaaattcg actatcttt 
      gcctatgga cagacgtct tcaggcaaa acttacaca 
      atgctcggg gacaagaag catagaggg atcatgtcc 
      ttggctatc gaaaatatt ttcgagcat attgaaaat 
      agtacagac cggaaattt ttaatcaga gtgtcgtac 
      attgaaatt tacaatgag aaaatttac gacctgctt 
      gatccctca aacaaggag gtgaagata cgcgaattc 
      ttccccgcg actatcggt ttacaaaac attaaggaa 
      gaaattgtt acaagcagg aagcaaatg tacgaatgt 
      cttaggacc ggaactttg aaccggcac attgccgga 
      accaaagcc aatgatagg agcagtcgg tcgcatact 
      attttcaag atcacgatt gaatccact caagtctcc 
      gattttact agcgggccc gttcaggtt tcaagcctc 
      aatttagtg gaccttgcg ggttccgag agggttgct 
      caaacgaag gccaccgga gtgcggctt aaggaaggc 
      agccacata aataagtca ctgtcagct ctgggtcta 
      gtgatcaga caactgagt gatggtcaa gaatttatc 
      aattttcgt gattcaaag ttaacaaga cttctgcaa 
      gactctctc ggcggtaac tccaaaact ttgattatt 
      gcaacaatc acactggcg tcgattgaa gatacgtgt 
      tccacgttg gcttttgca caacgagct aaagctgtg 
      aaaaataag ccacatgtc aacgaaatt ttaacagat 
      gctgacttg agtaaacgc tatgctagt ctgaactca 
      cagttgcaa aagaaattg gaagaacaa ctgcgaata 
      aaccagaaa ttacaagaa acacaagaa gtaaatgtg 
      agggaaatg agcagattg caacagcag atggaatta 
      attgaacac tttcagcga ggcaaaatt agtgaaatt 
      ccttctatt ccaaaaaga aggcacaca atcgggttt 
      gcaacacca atgccatta tcatccatt gtttttaaa 
      cctgactgt gatacatca aatcaagat ccacatagt 
      ttattgttt ttggacgaa ttctcttca aaagatcac 
      acagcttta ggaaatact cttgaaaca attgtggag 
      gaagacttt attacacca atgaaaagg aatttggaa 
      aaacagagg cgtagttct tatagcagt gtttgtagt 
      gaatttcca gttgaagat aacgatgca ttgaaaaag 
      attacggaa ctagaagaa gagatttat aatgagaaa 
      caaaagcaa caagaatgt gaaattcag ctgaaagaa 
      ttaaatgac aaatttaaa ttaagagaa gaggatctt 
      tgtaacaaa attttggaa ttagaaaaa aaattagaa 
      aatgaaaac tccgacaat ttctattct acaattatt 
      ggttttaaa gatcaatta ctttattta caacaaata 
      ctaagtaat gaaaaaatt gaaacagag ttttcgaat 
      tcatactcg gagaaagat ttgacaaat aagtcatat 
      tctttagaa ctgagtgag gtaaaagag tgtgatgag 
      ctacactta gaattaatg gataaggaa caagaagaa 
      agaaaatgt ttgagcatt ttagaagag aatgagaaa 
      ctgaaaatg gaattaatt cattacgaa actaaaaac 
      aaatttcta accgatgaa cttgataga ctaacaact 
      gaaattaaa caaaaacat actttgaag aattcaaat 
      gaaatggag ctgacactt gacgaatat gaattaaag 
      caggattac atcaaacta aagaacgaa ttcactgct 
      tcttgtaca gaaaacgaa tttttgcaa aaatccaat 
      gaagaaatg aaaaggaaa attgaaaaa tatgattct 
      ttaaagcaa gagtacata caattaaag aacgatttg 
      actacttct cgtacagag aacgattct ttgctaaag 
      ttaattgag gaaatgaag gggagaatt gaggaatat 
      cattcgtta aatattcaa ctgcagaac aaattgaaa 
      atgaacaga aaaactgaa gaatatgat tcgttatcg 
      caacagtac atccaacta aagaacgaa ttaacttct 
      tctagtaca gaaaaagag tttttgctg aagtcaaat 
      gaggaaatg agaaggaga attgaagaa tatgaattg 
      ttagagcaa caatacata caattcaag aacgaattc 
      acgactttg tgcacagaa aacgacgct ttgcaaaat 
      tcagttgag gaaatgaag aggggaatt gaagaatat 
      cagtcgtta aagtcggag tacattcaa cttcaggac 
      gaattcaat gctttctgt agtgagaaa gaatccttg 
      aaaaaatca aacgaggaa atgaaaaaa agaactgaa 
      gaatacgaa ttgttaaag cagcaatac atacaactg 
      aagaacgaa tttaacgct tcttgtacc gaaaaagat 
      tccttgcaa aaattaaac gaagaaata aaaaggata 
      gtcgaagaa aatgaatcg ttaaagcag gagtacatt 
      caattaaac aacaaattc aatgcttcg tgcactgaa 
      aaggactcc ttgcaaaaa tcaaatgaa gaaatgaag 
      cggaaaatt gaagaaaat gaatcgtta aaggaggaa 
      tacgtccaa ctgaagaac gaattttct gctacttgt 
      agagaaaaa gagtccttg caaaaatta aatgaagaa 
      atcaaaaga agaattgaa gaaagtgaa tcgttaaag 
      caggaatat atccagttg aagaacaaa tttgatgct 
      tcgtgcact gaaaaggac tccttgcaa aaatcaaat 
      gaagaaatg aagcggaaa attgaagaa aatgaatcg 
      ttaaaggag gaatacgtc caattgaag aacgaattt 
      tctactact tgtagggaa aaagagtcc ttgcataaa 
      ttaaacgaa gaaattaaa agaaaaatt gaagaaagt 
      gagtcgcta aaacaggag tatatccag ttgaagaac 
      aaattcaat gcttcgtgt acggaaaag gattccttg 
      ctaaaatca aacgaagaa atgaagagg aaaattgaa 
      gaaaatgaa tcgttgaag caggaatat gctcaactg 
      aagaacgaa ttatctgct tcttgcaga gaaaaagag 
      tccttgcaa aaattaatt gaagaaagt aagagagaa 
      gtcgaagaa agtgaatcg ttgaagcag gagcacatt 
      caattgaag atcaaattt aatgacttg tgtgctgaa 
      aaggactct tggcaaaaa tcaaatgaa gaaatgaag 
      cggaaaatt gaagaaaat gaatcgtta aaggaggaa 
      tacatccaa ctgaagaac gaattttct gctacttgt 
      agagaaaaa gagtccttg cataaatta aacgaaaaa 
      attaaaaga aaaattgaa gaaagtgaa tcgttaaag 
      caggagtac atcgagttt aagaacaaa ttcactgct 
      ttgtgtacg gaaaaggac tccttgcta aaacttgag 
      tacgcccaa ctgaagaac gaattcaaa gtttcgtgc 
      acagaaaaa gactctttg aaaaaatca aatgaagag 
      atgaggatg ataattgaa gaacaaaag aaaaaaata 
      gagaatatg aaaatatgt aacgacaaa ttaaattta 
      gaaattcgc aataaagaa acaaactca aaagaacag 
      ttgtgcact atgtctagt caaaataat tacttgaaa 
      aatgaactc gaaaaacta aaaaacgaa gttgctgac 
      caaaagcga attattagt gcgaacaag gaaaaattg 
      cattgtaac gacttggaa ttacaaaag caaaaacgt 
      cgtcttctg gaaggggaa gagcatgta aaagaattg 
      gaatacaaa ataaggcaa tcctccgag aaggagtcg 
      aattttcga acgataata gaagcacag aacgttaca 
      attgacaaa ttaaattta aagtatcgg aatatttta 
      ggtagtatt atggagtat tttatctct gagcagaag 
      tctttattg cttatccgt cattctcaa gacgaaaga 
      gaaaatttc aaaagtatt ataagagat ctcaaaaca 
      aataatgca actttgaaa agtcaggtt gagcaatta 
      cgaaacgaa aacaactgt gatttgtgt gcaattcaa 
      gaaactata aaaaaatct catctaatt ttcaaagat 
      tatttttct ttggaaaaa acatttctc aaagcccag 
      attattgac ttagaaaac accaacgaa gaatatttg 
      aaggaaatg atggctctt aaagatcag caacgaaac 
      gattcgaca gaagattgt tcaaattgt gataagttg 
      caaaacgaa attacaaga ctgactgag gtagcaacc 
      tctcaagat aaactggtt gttacatta gaaaaagaa 
      aattctacc ttgcggaaa gaacgattt gaatccgtg 
      aaacaggtt gttcattta caacaacaa atttttgac 
      gaacaagac aaaaagagt gaacttcaa tatacagag 
      tggatgaaa tttctccaa gataacaac aaattgcaa 
      caacaagaa attcgagag ttaaaacgc aagattaag 
      aaatatgaa aacatgcac agtcgcagt gtaaacaaa 
      cagtcagtt ggaacagaa tgtaattta cttggtgca 
      ccagcaacg gaaatcgat aaatccaaa aatgttaga 
      gtagaggac gtacaaaat ggctgtttg cattttagt 
      gggaagatc tctactata catgctgaa gttcagtgt 
      aacatattt agcgaacca tcggcgcta caagaaaag 
      tacgatctt ctgaaacaa atttatagg aaaaaaaat 
      aacgaaatc caatcactt aatgctgag ataaatcaa 
      cttaaatta aaagttagc agtttgtaa
    mRNA sequence
    • >TC014111 RA
      ttttaaacc acagaaatg tctacaaat atacaagta 
      gcaataaga atacgtccc ctcatcgcg agtgaggag 
      gccaaaaag cttcaggtg cagtggggc acccaaaag 
      aacaacatt ttccaaatt gacgacaat gggcaaaag 
      tttggagac gtcttcagc tttgaccac attttcgga 
      gttgataaa acaaacacg gacatttat gacaacatt 
      gtgaaggat tttgtggaa tcgtcttta aacggctta 
      aattcgact atctttgcc tatggacag acgtcttca 
      ggcaaaact tacacaatg ctcggggac aagaagcat 
      agagggatc atgtccttg gctatcgaa aatattttc 
      gagcatatt gaaaatagt acagaccgg aaattttta 
      atcagagtg tcgtacatt gaaatttac aatgagaaa 
      atttacgac ctgcttgat ccctcaaac aaggaggtg 
      aagatacgc gaattcttc cccgcgact atcggttta 
      caaaacatt aaggaagaa attgttaca agcaggaag 
      caaatgtac gaatgtctt aggaccgga actttgaac 
      cggcacatt gccggaacc aaagccaat gataggagc 
      agtcggtcg catactatt ttcaagatc acgattgaa 
      tccactcaa gtctccgat tttactagc gggcccgtt 
      caggtttca agcctcaat ttagtggac cttgcgggt 
      tccgagagg gttgctcaa acgaaggcc accggagtg 
      cggcttaag gaaggcagc cacataaat aagtcactg 
      tcagctctg ggtctagtg atcagacaa ctgagtgat 
      ggtcaagaa tttatcaat tttcgtgat tcaaagtta 
      acaagactt ctgcaagac tctctcggc ggtaactcc 
      aaaactttg attattgca acaatcaca ctggcgtcg 
      attgaagat acgtgttcc acgttggct tttgcacaa 
      cgagctaaa gctgtgaaa aataagcca catgtcaac 
      gaaatttta acagatgct gacttgagt aaacgctat 
      gctagtctg aactcacag ttgcaaaag aaattggaa 
      gaacaactg cgaataaac cagaaatta caagaaaca 
      caagaagta aatgtgagg gaaatgagc agattgcaa 
      cagcagatg gaattaatt gaacacttt cagcgaggc 
      aaaattagt gaaattcct tctattcca aaaagaagg 
      cacacaatc gggtttgca acaccaatg ccattatca 
      tccattgtt tttaaacct gactgtgat acatcaaat 
      caagatcca catagttta ttgtttttg gacgaattc 
      tcttcaaaa gatcacaca gctttagga aatactctt 
      gaaacaatt gtggaggaa gactttatt acaccaatg 
      aaaaggaat ttggaaaaa cagaggcgt agttcttat 
      agcagtgtt tgtagtgaa tttccagtt gaagataac 
      gatgcattg aaaaagatt acggaacta gaagaagag 
      atttataat gagaaacaa aagcaacaa gaatgtgaa 
      attcagctg aaagaatta aatgacaaa tttaaatta 
      agagaagag gatctttgt aacaaaatt ttggaatta 
      gaaaaaaaa ttagaaaat gaaaactcc gacaatttc 
      tattctaca attattggt tttaaagat caattactt 
      tatttacaa caaatacta agtaatgaa aaaattgaa 
      acagagttt tcgaattca tactcggag aaagatttg 
      acaaataag tcatattct ttagaactg agtgaggta 
      aaagagtgt gatgagcta cacttagaa ttaatggat 
      aaggaacaa gaagaaaga aaatgtttg agcatttta 
      gaagagaat gagaaactg aaaatggaa ttaattcat 
      tacgaaact aaaaacaaa tttctaacc gatgaactt 
      gatagacta acaactgaa attaaacaa aaacatact 
      ttgaagaat tcaaatgaa atggagctg acacttgac 
      gaatatgaa ttaaagcag gattacatc aaactaaag 
      aacgaattc actgcttct tgtacagaa aacgaattt 
      ttgcaaaaa tccaatgaa gaaatgaaa aggaaaatt 
      gaaaaatat gattcttta aagcaagag tacatacaa 
      ttaaagaac gatttgact acttctcgt acagagaac 
      gattctttg ctaaagtta attgaggaa atgaagggg 
      agaattgag gaatatcat tcgttaaat attcaactg 
      cagaacaaa ttgaaaatg aacagaaaa actgaagaa 
      tatgattcg ttatcgcaa cagtacatc caactaaag 
      aacgaatta acttcttct agtacagaa aaagagttt 
      ttgctgaag tcaaatgag gaaatgaga aggagaatt 
      gaagaatat gaattgtta gagcaacaa tacatacaa 
      ttcaagaac gaattcacg actttgtgc acagaaaac 
      gacgctttg caaaattca gttgaggaa atgaagagg 
      ggaattgaa gaatatcag tcgttaaag tcggagtac 
      attcaactt caggacgaa ttcaatgct ttctgtagt 
      gagaaagaa tccttgaaa aaatcaaac gaggaaatg 
      aaaaaaaga actgaagaa tacgaattg ttaaagcag 
      caatacata caactgaag aacgaattt aacgcttct 
      tgtaccgaa aaagattcc ttgcaaaaa ttaaacgaa 
      gaaataaaa aggatagtc gaagaaaat gaatcgtta 
      aagcaggag tacattcaa ttaaacaac aaattcaat 
      gcttcgtgc actgaaaag gactccttg caaaaatca 
      aatgaagaa atgaagcgg aaaattgaa gaaaatgaa 
      tcgttaaag gaggaatac gtccaactg aagaacgaa 
      ttttctgct acttgtaga gaaaaagag tccttgcaa 
      aaattaaat gaagaaatc aaaagaaga attgaagaa 
      agtgaatcg ttaaagcag gaatatatc cagttgaag 
      aacaaattt gatgcttcg tgcactgaa aaggactcc 
      ttgcaaaaa tcaaatgaa gaaatgaag cggaaaatt 
      gaagaaaat gaatcgtta aaggaggaa tacgtccaa 
      ttgaagaac gaattttct actacttgt agggaaaaa 
      gagtccttg cataaatta aacgaagaa attaaaaga 
      aaaattgaa gaaagtgag tcgctaaaa caggagtat 
      atccagttg aagaacaaa ttcaatgct tcgtgtacg 
      gaaaaggat tccttgcta aaatcaaac gaagaaatg 
      aagaggaaa attgaagaa aatgaatcg ttgaagcag 
      gaatatgct caactgaag aacgaatta tctgcttct 
      tgcagagaa aaagagtcc ttgcaaaaa ttaattgaa 
      gaaagtaag agagaagtc gaagaaagt gaatcgttg 
      aagcaggag cacattcaa ttgaagatc aaatttaat 
      gacttgtgt gctgaaaag gactcttgg caaaaatca 
      aatgaagaa atgaagcgg aaaattgaa gaaaatgaa 
      tcgttaaag gaggaatac atccaactg aagaacgaa 
      ttttctgct acttgtaga gaaaaagag tccttgcat 
      aaattaaac gaaaaaatt aaaagaaaa attgaagaa 
      agtgaatcg ttaaagcag gagtacatc gagtttaag 
      aacaaattc actgctttg tgtacggaa aaggactcc 
      ttgctaaaa cttgagtac gcccaactg aagaacgaa 
      ttcaaagtt tcgtgcaca gaaaaagac tctttgaaa 
      aaatcaaat gaagagatg aggatgata attgaagaa 
      caaaagaaa aaaatagag aatatgaaa atatgtaac 
      gacaaatta aatttagaa attcgcaat aaagaaaca 
      aactcaaaa gaacagttg tgcactatg tctagtcaa 
      aataattac ttgaaaaat gaactcgaa aaactaaaa 
      aacgaagtt gctgaccaa aagcgaatt attagtgcg 
      aacaaggaa aaattgcat tgtaacgac ttggaatta 
      caaaagcaa aaacgtcgt cttctggaa ggggaagag 
      catgtaaaa gaattggaa tacaaaata aggcaatcc 
      tccgagaag gagtcgaat tttcgaacg ataatagaa 
      gcacagaac gttacaatt gacaaatta aatttaaag 
      tatcggaat attttaggt agtattatg gagtatttt 
      atctctgag cagaagtct ttattgctt atccgtcat 
      tctcaagac gaaagagaa aatttcaaa agtattata 
      agagatctc aaaacaaat aatgcaact ttgaaaagt 
      caggttgag caattacga aacgaaaac aactgtgat 
      ttgtgtgca attcaagaa actataaaa aaatctcat 
      ctaattttc aaagattat ttttctttg gaaaaaaca 
      tttctcaaa gcccagatt attgactta gaaaacacc 
      aacgaagaa tatttgaag gaaatgatg gctcttaaa 
      gatcagcaa cgaaacgat tcgacagaa gattgttca 
      aattgtgat aagttgcaa aacgaaatt acaagactg 
      actgaggta gcaacctct caagataaa ctggttgtt 
      acattagaa aaagaaaat tctaccttg cggaaagaa 
      cgatttgaa tccgtgaaa caggttgtt catttacaa 
      caacaaatt tttgacgaa caagacaaa aagagtgaa 
      cttcaatat acagagtgg atgaaattt ctccaagat 
      aacaacaaa ttgcaacaa caagaaatt cgagagtta 
      aaacgcaag attaagaaa tatgaaaac atgcacagt 
      cgcagtgta aacaaacag tcagttgga acagaatgt 
      aatttactt ggtgcacca gcaacggaa atcgataaa 
      tccaaaaat gttagagta gaggacgta caaaatggc 
      tgtttgcat tttagtggg aagatctct actatacat 
      gctgaagtt cagtgtaac atatttagc gaaccatcg 
      gcgctacaa gaaaagtac gatcttctg aaacaaatt 
      tataggaaa aaaaataac gaaatccaa tcacttaat 
      gctgagata aatcaactt aaattaaaa gttagcagt 
      ttgtaattt tttatatag ctacttaac aaatgttaa 
      atatacata aataaatat tattttcga ct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY