Status
Please wait ...
Gene information
General information
TC014098 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5611866..5634852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014098
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05557

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014098 RA
      MEEQKSSGQ MKNLCWVFS SFRDSALEK LYQSFSVRQ 
      KRAGLECFL ITAILFDIY MLVVPSDQD FVIIAVMGT 
      FLIVNSFLL IWCKKGFQN KPLWTVVPH IAFHIANTQ 
      ILAGLFLKK NEVTPRDSL GWFLLLTYL IYVTLPLRL 
      PFCVLLSIG TFTSYIVSL CGLTKSQLH FVEQQVSNC 
      ILLVTANIL GLTSYLVED KQQRRAFLE TRQSLEMKL 
      VIEEQSAEQ ERLLLSVLP EHVAVQMRQ DLDQADSQF 
      KKIYMSRHE NVSILYADI VGFTAISST YSAQELVKM 
      LNELFARFD KLAEKYQQL RIKILGDCY YCISGAPRE 
      RPDHAVLCV HMGLSMVKA IKYVQQTAN SPVDMRVGI 
      HTGAVLAGV LGQRQWQFD VYSKDVELA NKMESSGMA 
      GRVHISATT YSFLNGEFE VEPGYGEKR EEALRIAGL 
      KTYFIVKVL KPFQPPVTE VQNGSSILP EEDSCVIAE 
      ISDTKDGDV DDGNQKRSR EDSRDFKIR LRTELVNRD 
      GHKELSKNT TCLTLNFVD EKKEKLYQQ QVETFSSIT 
      LLMFLVVRL SIGLTVFTV LPRDMSINI SYCTENVII 
      IVLVSILIC DEFLPLKCW NAFKDSVTV RRILSAICV 
      FLLGTVNLI DTISCVQFH SNDNCTFTD DINSSCLYP 
      SYFSYFAVL ILIASSLPA CLSYLWKAL FLFIIALAQ 
      CLVNIFVLE LVLECEESR KNWKSLTKG KYTLSALLL 
      IATLALAFL ARHMEQVAR VLFLWRCEV EEQRDFAED 
      MRRRNEALV YNILPPHVA AHFMGNRKR QHDELYSQS 
      YAEIGVLFA SMPNFSDFY SEETVNNQG LECLRFLNE 
      VISDFDALL ELPQFQDII KIKTIGSTY MAASGLNPT 
      RQVKPDDPI TVRWAHLAL LVEFALELK KALQGINEQ 
      SFNHFVLKL GINHGPITA GVIGARKPH YDIWGNTVN 
      VASRMESTG KAGCIQVTE ETCEILQHF GYQFEQRGL 
      VAVKGKGQL MTYYLLGKA GKITPPTAP VSPIIGVTA 
      METVNEEDE SHDSPTSKS EDKTVIECT DQKIEDEVF 
      TTDTEQNFS TKEAEPLLQ C
    CDS sequence
    • >TC014098 RA
      atggaggag caaaaatcg tcaggacag atgaaaaac 
      ctgtgctgg gttttcagc agctttcgc gattcggca 
      ctcgaaaag ctttaccag agtttcagc gtgaggcag 
      aagcgcgct gggttggaa tgtttcctg ataacggca 
      attctcttc gatatttac atgttggtg gttcccagc 
      gaccaggat tttgtcata atcgcagtg atgggcaca 
      tttctcatt gtcaattct tttctgcta atctggtgt 
      aagaagggg ttccaaaac aagccgctg tggactgtg 
      gtaccgcac attgctttc cacattgcc aacactcag 
      atactcgcc gggctgttc ttgaagaag aacgaagtg 
      acacccaga gatagtcta ggctggttt ttgctccta 
      acttactta atttatgtc acactaccg ctaagactg 
      cctttttgc gttctgctc agcattgga acgttcact 
      tcgtacatc gtttcgctg tgcggattg accaaatca 
      caacttcat ttcgtggag cagcaagtc tccaactgc 
      atcttgttg gttactgca aatatcttg gggctgaca 
      tcttatctt gtcgaagat aaacagcag cgacgggcc 
      ttcttagaa actagacaa tcattggaa atgaaactt 
      gttatagag gagcaaagt gccgaacag gaacgttta 
      cttctatcc gttctcccg gaacatgtg gcggtacaa 
      atgagacag gatttggac caagccgac agccaattt 
      aagaaaatt tacatgagc aggcacgaa aacgtaagt 
      attttgtat gccgacatc gtgggattt acagcgatc 
      tcatcaaca tattcagct caagagctt gtaaaaatg 
      ctgaatgaa ttatttgca agattcgac aaacttgcc 
      gagaaatac caacagctg agaataaag attttagga 
      gactgctat tattgcatt agtggtgcg ccgagggag 
      cgtccagat catgcagtt ctttgtgtt catatggga 
      ctgtcgatg gtcaaagcg ataaaatat gtacagcaa 
      acagcgaat tctcctgta gatatgaga gttggaatt 
      cacacggga gcggttcta gcgggagtt ctgggccag 
      agacaatgg caattcgac gtctactcg aaggacgtt 
      gaattagcc aacaaaatg gaaagcagc ggcatggca 
      ggacgagtg catatttct gccacaacc tattcgttc 
      cttaatggt gagttcgaa gtcgaacct ggttatggc 
      gagaagcgg gaagaagca ctgcgaata gcaggatta 
      aaaacctac ttcatcgtc aaagtttta aaaccattt 
      caaccgcct gttacggaa gttcagaat gggagtagt 
      atacttcca gaagaggac agttgtgta attgcagaa 
      atttcagac acaaaggat ggagatgtt gacgatgga 
      aaccaaaaa agatcgcgg gaagactcg cgagacttt 
      aagatcaga ttaagaaca gaactggtt aacagagac 
      ggtcataaa gagttgtct aagaacacg acatgtttg 
      actcttaat ttcgtggac gaaaagaag gaaaagttg 
      tatcaacag caggtcgag acattttca agcataaca 
      ctgctaatg tttctcgta gttcgcctg tccattggc 
      ttaacggtt ttcactgtc ttaccaagg gacatgtca 
      atcaatata tcgtactgt acagaaaat gtaataata 
      atagtcctc gtctccatc ctcatttgc gacgaattt 
      ttgccgctg aaatgctgg aacgctttt aaggattca 
      gtaacagtt cgtcggatc ctgtcggca atttgtgtc 
      tttctttta ggaacggtc aatctaata gacacaatt 
      tcttgtgtt cagttccat tcgaacgat aattgcacg 
      ttcactgac gatataaat tcgtcatgt ctctatccc 
      tcctatttc agttatttc gccgttcta attctgatc 
      gcttcctct cttccagcc tgcctctca tatctctgg 
      aaggctctg tttctgttt atcatagct ctggctcaa 
      tgcctcgtg aacattttc gtcttagaa ttagtactc 
      gagtgcgag gagtcgcgg aaaaactgg aaaagctta 
      actaagggt aaatacacg ctttcagcg ttgttgctc 
      attgccacg ctcgctttg gctttcctt gcgagacat 
      atggagcag gtggcgaga gttttgttc ttatggcgg 
      tgcgaggtc gaggagcaa cgcgatttc gccgaggat 
      atgagaaga agaaatgaa gctctggtg tataatatc 
      cttccgcca catgtagcg gcccatttt atggggaac 
      aggaagagg caacacgat gaactttac tcgcaaagt 
      tacgccgag attggggtg ctttttgcc tcgatgccc 
      aacttttct gacttctat tcagaggag accgtcaac 
      aatcagggc ttagaatgt ctgcgattt ctgaacgaa 
      gtcatttca gatttcgat gctctttta gagctacct 
      cagtttcaa gacataata aaaattaag acaatcggt 
      tctacgtac atggcagca agcggccta aacccgact 
      cgacaagtc aaacccgac gaccccata acagtgcga 
      tgggcgcat cttgccctc cttgttgaa ttcgcactg 
      gagctgaag aaggcctta cagggcatc aacgaacaa 
      agttttaac cattttgtt ttaaaatta ggtatcaac 
      catgggcct atcacagcg ggagtaatc ggagctaga 
      aaaccccac tacgacatc tggggcaac acggtcaat 
      gtagcttcc aggatggaa agtaccgga aaagctgga 
      tgtattcag gtgactgaa gagacgtgc gaaatcctg 
      cagcacttc ggctaccag ttcgagcag cgcggccta 
      gtggcagtg aagggaaaa ggccaactg atgacctac 
      tacttgctc gggaaagcc gggaaaatc acccctcct 
      acggctccg gtctctcca atcataggt gtcaccgca 
      atggaaact gtaaacgaa gaggacgaa tcgcacgat 
      agtcctacg tccaaatcc gaggataaa acagtaatc 
      gaatgcacc gaccagaaa atcgaagac gaagttttc 
      acaacggac accgagcag aattttagt accaaagaa 
      gcggaaccg ctgctgcag tgttaa
    mRNA sequence
    • >TC014098 RA
      gtccggtgc ttggtggaa tcgatctta cgtggtggc 
      ataagttca cgtgcagtg aaacgttcg ttcctctca 
      tgataacaa aagagctga atcttgccg cctcagcac 
      ggccctcta ccccgcacg gaatttttt tcagaaaaa 
      tgtaaaact gagtaaaaa agaatgaca acagaaacg 
      gcttatata ttttcataa gtaacgaat attttttaa 
      acgaaatgg aggagcaaa aatcgtcag gacagatga 
      aaaacctgt gctgggttt tcagcagct ttcgcgatt 
      cggcactcg aaaagcttt accagagtt tcagcgtga 
      ggcagaagc gcgctgggt tggaatgtt tcctgataa 
      cggcaattc tcttcgata tttacatgt tggtggttc 
      ccagcgacc aggattttg tcataatcg cagtgatgg 
      gcacatttc tcattgtca attcttttc tgctaatct 
      ggtgtaaga aggggttcc aaaacaagc cgctgtgga 
      ctgtggtac cgcacattg ctttccaca ttgccaaca 
      ctcagatac tcgccgggc tgttcttga agaagaacg 
      aagtgacac ccagagata gtctaggct ggtttttgc 
      tcctaactt acttaattt atgtcacac taccgctaa 
      gactgcctt tttgcgttc tgctcagca ttggaacgt 
      tcacttcgt acatcgttt cgctgtgcg gattgacca 
      aatcacaac ttcatttcg tggagcagc aagtctcca 
      actgcatct tgttggtta ctgcaaata tcttggggc 
      tgacatctt atcttgtcg aagataaac agcagcgac 
      gggccttct tagaaacta gacaatcat tggaaatga 
      aacttgtta tagaggagc aaagtgccg aacaggaac 
      gtttacttc tatccgttc tcccggaac atgtggcgg 
      tacaaatga gacaggatt tggaccaag ccgacagcc 
      aatttaaga aaatttaca tgagcaggc acgaaaacg 
      taagtattt tgtatgccg acatcgtgg gatttacag 
      cgatctcat caacatatt cagctcaag agcttgtaa 
      aaatgctga atgaattat ttgcaagat tcgacaaac 
      ttgccgaga aataccaac agctgagaa taaagattt 
      taggagact gctattatt gcattagtg gtgcgccga 
      gggagcgtc cagatcatg cagttcttt gtgttcata 
      tgggactgt cgatggtca aagcgataa aatatgtac 
      agcaaacag cgaattctc ctgtagata tgagagttg 
      gaattcaca cgggagcgg ttctagcgg gagttctgg 
      gccagagac aatggcaat tcgacgtct actcgaagg 
      acgttgaat tagccaaca aaatggaaa gcagcggca 
      tggcaggac gagtgcata tttctgcca caacctatt 
      cgttcctta atggtgagt tcgaagtcg aacctggtt 
      atggcgaga agcgggaag aagcactgc gaatagcag 
      gattaaaaa cctacttca tcgtcaaag ttttaaaac 
      catttcaac cgcctgtta cggaagttc agaatggga 
      gtagtatac ttccagaag aggacagtt gtgtaattg 
      cagaaattt cagacacaa aggatggag atgttgacg 
      atggaaacc aaaaaagat cgcgggaag actcgcgag 
      actttaaga tcagattaa gaacagaac tggttaaca 
      gagacggtc ataaagagt tgtctaaga acacgacat 
      gtttgactc ttaatttcg tggacgaaa agaaggaaa 
      agttgtatc aacagcagg tcgagacat tttcaagca 
      taacactgc taatgtttc tcgtagttc gcctgtcca 
      ttggcttaa cggttttca ctgtcttac caagggaca 
      tgtcaatca atatatcgt actgtacag aaaatgtaa 
      taataatag tcctcgtct ccatcctca tttgcgacg 
      aatttttgc cgctgaaat gctggaacg cttttaagg 
      attcagtaa cagttcgtc ggatcctgt cggcaattt 
      gtgtctttc ttttaggaa cggtcaatc taatagaca 
      caatttctt gtgttcagt tccattcga acgataatt 
      gcacgttca ctgacgata taaattcgt catgtctct 
      atccctcct atttcagtt atttcgccg ttctaattc 
      tgatcgctt cctctcttc cagcctgcc tctcatatc 
      tctggaagg ctctgtttc tgtttatca tagctctgg 
      ctcaatgcc tcgtgaaca ttttcgtct tagaattag 
      tactcgagt gcgaggagt cgcggaaaa actggaaaa 
      gcttaacta agggtaaat acacgcttt cagcgttgt 
      tgctcattg ccacgctcg ctttggctt tccttgcga 
      gacatatgg agcaggtgg cgagagttt tgttcttat 
      ggcggtgcg aggtcgagg agcaacgcg atttcgccg 
      aggatatga gaagaagaa atgaagctc tggtgtata 
      atatccttc cgccacatg tagcggccc attttatgg 
      ggaacagga agaggcaac acgatgaac tttactcgc 
      aaagttacg ccgagattg gggtgcttt ttgcctcga 
      tgcccaact tttctgact tctattcag aggagaccg 
      tcaacaatc agggcttag aatgtctgc gatttctga 
      acgaagtca tttcagatt tcgatgctc ttttagagc 
      tacctcagt ttcaagaca taataaaaa ttaagacaa 
      tcggttcta cgtacatgg cagcaagcg gcctaaacc 
      cgactcgac aagtcaaac ccgacgacc ccataacag 
      tgcgatggg cgcatcttg ccctccttg ttgaattcg 
      cactggagc tgaagaagg ccttacagg gcatcaacg 
      aacaaagtt ttaaccatt ttgttttaa aattaggta 
      tcaaccatg ggcctatca cagcgggag taatcggag 
      ctagaaaac cccactacg acatctggg gcaacacgg 
      tcaatgtag cttccagga tggaaagta ccggaaaag 
      ctggatgta ttcaggtga ctgaagaga cgtgcgaaa 
      tcctgcagc acttcggct accagttcg agcagcgcg 
      gcctagtgg cagtgaagg gaaaaggcc aactgatga 
      cctactact tgctcggga aagccggga aaatcaccc 
      ctcctacgg ctccggtct ctccaatca taggtgtca 
      ccgcaatgg aaactgtaa acgaagagg acgaatcgc 
      acgatagtc ctacgtcca aatccgagg ataaaacag 
      taatcgaat gcaccgacc agaaaatcg aagacgaag 
      ttttcacaa cggacaccg agcagaatt ttagtacca 
      aagaagcgg aaccgctgc tgcagtgtt aatgagata 
      gtggataga caaagcact gattatttg taggctgtc 
      atttttaaa tcatttttg tacttcgag aaatcattc 
      gaagtggtg tttagaaat cgatagatt aagactaca 
      tgtctaagt atacacaat cagatttta aagtactcg 
      taggtaata acgaataaa atatttaag cagagtacc 
      gtcatagac gtagaaatt ttttgtaaa agttatttt 
      ctgctagcg tcggttgta atttttctt ttctagaac 
      aaacgcaca tatctatta gaccattgt gttattgta 
      gtcgtagct acaattctt tttcaaaag ggcgggatt 
      atgaagcac aaaactata gaaattata aaaaatcgc 
      gcccttttc gagtggtgt tataactac agactcatt 
      ttaaaagca aatgcctaa gttgtactt cgaaattgt 
      tttgtaatt aagtacaaa ttaattaac ttccaaagg 
      gtaatttct gtgaaatgt gaaaatctt aaaccttag 
      aattacaaa gcgagctca aaatattct tctactagg 
      tatattgta tgaatttca agaacggga tcaagtagg 
      agcttgacg ataattttt aaaagaagc aattgcttt 
      cagttgtaa ataatcatt tttgtttac attgttatt 
      ttattttag atattacta tttcaaatg ttaggaaaa 
      tgtgtaaca atgtttagt aattaccag aaataaact 
      ttacactag gca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY