Status
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Gene information
General information
TC014089 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5549980..5555647
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014089
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05554

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014089 RA
      MESKHAVKV DQLKKHPKE NSNLLSDLF YCWTFPMIK 
      TGLKKALSE KDIYQTLTS HRSTNLTNK LERAWNSQK 
      NGSNKPSLW RAIWKVFKW DIMLSIFLY FVTDIVLKI 
      IRLIALGIL LGYYSPNAT EMSKSDAII CASVIVTSS 
      FLLEILEHA YILHFQQLA MKVRVACAS LIYRKALKL 
      SKKALSETT IGHIINLLS NDMQRLVAV WGAYHALWA 
      APLQAALIL FLLYWIAGP TALVGNIFL LVLTPLQVF 
      MTKKTADYR LKISLKSDE RIRYMSEII SGIQVIKVY 
      TWELPFVKL IDAIRRKEM YFIKIANFL DSITIAFEF 
      FFDRTSVFI CLVTYVLLG STPDAQYVF VLASFYDLM 
      MQSVSLYFP QGLTITLQA NVAIKRFEN FLNLHEIQN 
      KIITTKETG ITIDQVSAK WSETSQQNT LSDIKFNLE 
      PKQLVAIIG PIGSGKSSL LQLCLGELA PNEGCVKIG 
      GRISYANQE PWLFAGSVK QNILFGQAM VREKYQEVI 
      RVCALEDDI AQFPYGDNT IVGERGILL SGGQKARIN 
      LARAIYKDA DIYLLDDPL SAVDARVGK QIFNNCIMN 
      YLKGKCTVL VTHQIQYLS FVDKIYLMV DGKVAVSGS 
      YKELQASGE DFTRLLKEH EKYDESEDE SVVESKASK 
      EDKEQDEEE ETWIQGKVS KKVYISYLR ASGNYFRFA 
      VMGMLFIMT EILATGSDY FITFWVNLE QKRTAENKT 
      KNETLSSDP VDSFFTNEN CIYVYTAII GALILFSIL 
      RSMSFFQSC MKASVRLHD KMFTSVINA TMKFFYTNS 
      SGRILNRFA KDMGSIDET LPEVFLDAL QMSMAIIGT 
      VLAICIVSP WTLIPSIII VIIMLLLRL VYLSTTRDV 
      KRMESTNRS PIFAHLTES MKGLTTIRA YNTQGILEK 
      EFDTYQDVH TSIYYMYLG GNRALAVYL DIVCVLYVF 
      CITVIALTK ETYAGNVGF MITQGMSMS GMFQWGIRQ 
      WSEMENQMT SVERVGEYI DVEREKDTK TRDPPRQWP 
      EHGKIEFKS VSMRYSSND PYVLKNLNI SITPREKVG 
      IVGRTGAGK SSLIAVLFR LVDFEGRLI IDDCDTKEL 
      SLPALRSKI SIIPQEPIL FSGTVRKNL DPFDQYQDD 
      QIWSVLEKV KLKEFVASS DLGLHSNLA EGGSNFSVG 
      QKQLICLAR ALLRDSKIL ILDEATANV DPHTDELLQ 
      KTIRENFEN CTVLTIAHR LHTVMDSDK VLVMDDGKA 
      VEFDHPHAL LQKKGVFFD LVMETGKAM GANLSEIAQ 
      ENYLKRQNL LAQ
    CDS sequence
    • >TC014089 RA
      atggaatcg aaacatgcc gtcaaagtt gatcagtta 
      aagaaacat cctaaggaa aactcgaat ctcttgtct 
      gatctcttt tactgctgg acattccca atgattaag 
      acaggttta aaaaaggca ttatcggag aaagatatt 
      tatcaaact ctgaccagt catcggtcc acaaatttg 
      acaaataaa ctagagcga gcatggaat agtcagaaa 
      aatggaagc aataaaccc tctctttgg agagcaatt 
      tggaaggtt tttaaatgg gacataatg ctttctata 
      tttctttat ttcgtaaca gacattgtt ctcaaaatc 
      atccgtctt atagctcta ggaatactg ttgggttac 
      tactctcct aacgcaaca gaaatgagc aaaagcgac 
      gcaattatt tgcgcttca gttatagta acttcgagt 
      tttcttctc gaaatatta gaacatgcc tacatactt 
      catttccaa caactggca atgaaagta cgcgtggcc 
      tgtgcttct cttatttat cgaaaagct ctaaagctt 
      agtaagaag gcattaagt gaaacgacc atagggcat 
      attatcaat ttattgtca aatgacatg cagaggcta 
      gtcgctgtt tggggagct tatcatgct ctttgggca 
      gcgcctctt caggcagcc cttatcctt tttctactg 
      tactggatc gcaggacca acggcttta gttggaaat 
      atcttcctt ctagtactc acaccgttg caggtgttc 
      atgactaaa aaaactgcc gattatcga ttaaaaata 
      tctttaaaa agcgacgaa cgaatacgt tatatgagc 
      gaaatcata tcaggaatt caagtaatc aaggtttac 
      acctgggag ttacctttt gtcaaactt atagacgct 
      attagaagg aaagaaatg tattttata aaaattgcc 
      aacttctta gactcaatc acaatcgcc tttgaattc 
      ttctttgat cgcacttct gtatttatt tgcctagtg 
      acttacgtt ttgcttggg agtacccca gatgcccag 
      tatgttttt gtccttgcc tcattctat gatttgatg 
      atgcagtca gtttcacta tactttccc caagggttg 
      acaataact cttcaagca aatgtggcc attaaaaga 
      tttgaaaac ttcctcaac ttgcacgaa atacaaaac 
      aagataatc acaactaaa gaaactgga attacgata 
      gaccaagtt tccgcgaaa tggtctgaa acttcccaa 
      caaaacact ttatcagac atcaaattt aacttggag 
      ccaaaacaa ctagtcgcg ataattgga cccattggt 
      agtgggaaa tccagtctc ttgcagtta tgtctaggt 
      gagttggca cctaacgaa ggttgtgta aaaattggc 
      ggtcgaata tcgtacgcc aaccaggag ccttggctg 
      tttgccggt tctgtcaaa caaaacata cttttcggg 
      caagcgatg gttagagag aagtaccaa gaagtgatt 
      agagtttgt gctctggag gacgatatt gcccagttc 
      ccgtacggc gataacaca atcgtaggc gaacgcgga 
      attcttcta agtggtggt caaaaggcc agaataaac 
      ctcgccagg gcaatttac aaagatgct gatatttat 
      ttacttgac gatccttta tcggcagtg gacgcacgc 
      gttggcaaa caaattttc aacaactgt attatgaat 
      tatttaaaa ggcaaatgc acagttctt gttactcat 
      caaattcag tacttaagt ttcgtcgat aagatatat 
      ttgatggta gatggaaaa gttgcggtt tctggaagt 
      tacaaagag ttgcaagcg tcaggtgaa gactttact 
      agactttta aaagaacat gaaaaatac gacgaaagt 
      gaggacgaa tccgttgtt gaaagtaaa gcaagtaaa 
      gaggataaa gagcaagac gaagaagaa gagacttgg 
      attcaagga aaagtgtcc aagaaagtg tacatttcg 
      tatctccgt gcctctgga aattatttc aggtttgct 
      gtaatggga atgttgttt atcatgacc gagattttg 
      gccactggt tctgattac tttattact ttctgggtg 
      aatttggaa caaaaacga acagcggaa aacaaaacc 
      aaaaatgag actttatca tcagatcct gtagattct 
      ttctttaca aatgaaaac tgtatttac gtttacaca 
      gctataatt ggggccctg atattgttc tccattcta 
      cgttcaatg tcttttttc caaagttgc atgaaagcg 
      tctgtccgc ttgcacgac aaaatgttt actagcgta 
      ataaatgcc actatgaaa tttttctac accaattct 
      tctggaaga atactcaac agatttgcc aaagacatg 
      gggagcata gacgagact ttacctgaa gtgtttttg 
      gatgctctt cagatgtca atggcaatt attggcacg 
      gtcttagcc atatgcatt gtgagtccc tggactctc 
      atccccagt atcataatt gttataata atgttgctt 
      cttagatta gtttacctt tctaccact agagacgtg 
      aaacggatg gaatcaaca aaccggagt ccaattttt 
      gcacattta actgagtca atgaagggt ttgacgaca 
      ataagagcc tacaacacg caagggatc ttagagaag 
      gagttcgat acttatcag gacgtacac acttctatc 
      tattacatg tatttgggc ggcaacaga gccctggcc 
      gtttatttg gacatagtt tgtgtcctt tatgttttc 
      tgcatcacg gtcattgct ttgacaaaa gaaacctac 
      gcgggaaac gtcggtttt atgataact cagggtatg 
      agtatgagt ggtatgttt caatggggg atacgacag 
      tggagcgaa atggagaat caaatgact tcggttgaa 
      cgtgtcggg gaatacatc gatgttgaa cgtgaaaaa 
      gacactaaa actagagac cccccacga cagtggcct 
      gaacacggc aaaatcgaa ttcaagtct gtctccatg 
      agatattcc tcaaacgat ccatacgta ctcaaaaat 
      ctcaatatc tcgatcaca cctagagaa aaagtggga 
      attgtgggt cgaactgga gccggaaaa tcgtctcta 
      atcgcagtg ttgttccgt ttggttgat ttcgagggc 
      agacttatc attgatgac tgtgatacg aaagaattg 
      agtttgcca gcgttacgc tccaaaatt tcaatcatt 
      ccgcaagaa cctatccta ttttcggga acagtgcgt 
      aaaaactta gaccccttt gatcaatat caagacgac 
      caaatatgg agcgtactg gagaaagta aaattgaag 
      gaattcgtt gccagttct gatctagga ttacacagc 
      aatttggca gaaggcgga tcaaatttt agcgttggt 
      cagaagcaa ctgatttgt ctggctcga gcgttactg 
      agagacagc aaaattttg attcttgat gaagctacc 
      gctaatgtt gatccacac actgatgaa ctgttgcag 
      aagactatt agggaaaat ttcgaaaat tgtacagtt 
      ctcactatt gcacatcgg ttacatact gtaatggat 
      tccgacaag gttcttgta atggatgac ggaaaagcg 
      gttgagttt gatcacccc cacgcattg ttacaaaag 
      aagggagtt ttctttgat ttagtcatg gagacgggg 
      aaagcaatg ggtgccaat ttgtcagaa attgcccaa 
      gagaattac ttgaagagg cagaatctt ttagcacaa 
      tag
    mRNA sequence
    • >TC014089 RA
      ttattcgaa atacactag ttatcagtt agttgtata 
      gtttgcaac atttagtgt atgcagtat tagctcatc 
      tacgcagct ttaagtgat ttccttgtt atttgatct 
      catctaatt atttataat ggaatcgaa acatgccgt 
      caaagttga tcagttaaa gaaacatcc taaggaaaa 
      ctcgaatct cttgtctga tctctttta ctgctggac 
      attcccaat gattaagac aggtttaaa aaaggcatt 
      atcggagaa agatattta tcaaactct gaccagtca 
      tcggtccac aaatttgac aaataaact agagcgagc 
      atggaatag tcagaaaaa tggaagcaa taaaccctc 
      tctttggag agcaatttg gaaggtttt taaatggga 
      cataatgct ttctatatt tctttattt cgtaacaga 
      cattgttct caaaatcat ccgtcttat agctctagg 
      aatactgtt gggttacta ctctcctaa cgcaacaga 
      aatgagcaa aagcgacgc aattatttg cgcttcagt 
      tatagtaac ttcgagttt tcttctcga aatattaga 
      acatgccta catacttca tttccaaca actggcaat 
      gaaagtacg cgtggcctg tgcttctct tatttatcg 
      aaaagctct aaagcttag taagaaggc attaagtga 
      aacgaccat agggcatat tatcaattt attgtcaaa 
      tgacatgca gaggctagt cgctgtttg gggagctta 
      tcatgctct ttgggcagc gcctcttca ggcagccct 
      tatcctttt tctactgta ctggatcgc aggaccaac 
      ggctttagt tggaaatat cttccttct agtactcac 
      accgttgca ggtgttcat gactaaaaa aactgccga 
      ttatcgatt aaaaatatc tttaaaaag cgacgaacg 
      aatacgtta tatgagcga aatcatatc aggaattca 
      agtaatcaa ggtttacac ctgggagtt accttttgt 
      caaacttat agacgctat tagaaggaa agaaatgta 
      ttttataaa aattgccaa cttcttaga ctcaatcac 
      aatcgcctt tgaattctt ctttgatcg cacttctgt 
      atttatttg cctagtgac ttacgtttt gcttgggag 
      taccccaga tgcccagta tgtttttgt ccttgcctc 
      attctatga tttgatgat gcagtcagt ttcactata 
      ctttcccca agggttgac aataactct tcaagcaaa 
      tgtggccat taaaagatt tgaaaactt cctcaactt 
      gcacgaaat acaaaacaa gataatcac aactaaaga 
      aactggaat tacgataga ccaagtttc cgcgaaatg 
      gtctgaaac ttcccaaca aaacacttt atcagacat 
      caaatttaa cttggagcc aaaacaact agtcgcgat 
      aattggacc cattggtag tgggaaatc cagtctctt 
      gcagttatg tctaggtga gttggcacc taacgaagg 
      ttgtgtaaa aattggcgg tcgaatatc gtacgccaa 
      ccaggagcc ttggctgtt tgccggttc tgtcaaaca 
      aaacatact tttcgggca agcgatggt tagagagaa 
      gtaccaaga agtgattag agtttgtgc tctggagga 
      cgatattgc ccagttccc gtacggcga taacacaat 
      cgtaggcga acgcggaat tcttctaag tggtggtca 
      aaaggccag aataaacct cgccagggc aatttacaa 
      agatgctga tatttattt acttgacga tcctttatc 
      ggcagtgga cgcacgcgt tggcaaaca aattttcaa 
      caactgtat tatgaatta tttaaaagg caaatgcac 
      agttcttgt tactcatca aattcagta cttaagttt 
      cgtcgataa gatatattt gatggtaga tggaaaagt 
      tgcggtttc tggaagtta caaagagtt gcaagcgtc 
      aggtgaaga ctttactag acttttaaa agaacatga 
      aaaatacga cgaaagtga ggacgaatc cgttgttga 
      aagtaaagc aagtaaaga ggataaaga gcaagacga 
      agaagaaga gacttggat tcaaggaaa agtgtccaa 
      gaaagtgta catttcgta tctccgtgc ctctggaaa 
      ttatttcag gtttgctgt aatgggaat gttgtttat 
      catgaccga gattttggc cactggttc tgattactt 
      tattacttt ctgggtgaa tttggaaca aaaacgaac 
      agcggaaaa caaaaccaa aaatgagac tttatcatc 
      agatcctgt agattcttt ctttacaaa tgaaaactg 
      tatttacgt ttacacagc tataattgg ggccctgat 
      attgttctc cattctacg ttcaatgtc ttttttcca 
      aagttgcat gaaagcgtc tgtccgctt gcacgacaa 
      aatgtttac tagcgtaat aaatgccac tatgaaatt 
      tttctacac caattcttc tggaagaat actcaacag 
      atttgccaa agacatggg gagcataga cgagacttt 
      acctgaagt gtttttgga tgctcttca gatgtcaat 
      ggcaattat tggcacggt cttagccat atgcattgt 
      gagtccctg gactctcat ccccagtat cataattgt 
      tataataat gttgcttct tagattagt ttacctttc 
      taccactag agacgtgaa acggatgga atcaacaaa 
      ccggagtcc aatttttgc acatttaac tgagtcaat 
      gaagggttt gacgacaat aagagccta caacacgca 
      agggatctt agagaagga gttcgatac ttatcagga 
      cgtacacac ttctatcta ttacatgta tttgggcgg 
      caacagagc cctggccgt ttatttgga catagtttg 
      tgtccttta tgttttctg catcacggt cattgcttt 
      gacaaaaga aacctacgc gggaaacgt cggttttat 
      gataactca gggtatgag tatgagtgg tatgtttca 
      atgggggat acgacagtg gagcgaaat ggagaatca 
      aatgacttc ggttgaacg tgtcgggga atacatcga 
      tgttgaacg tgaaaaaga cactaaaac tagagaccc 
      cccacgaca gtggcctga acacggcaa aatcgaatt 
      caagtctgt ctccatgag atattcctc aaacgatcc 
      atacgtact caaaaatct caatatctc gatcacacc 
      tagagaaaa agtgggaat tgtgggtcg aactggagc 
      cggaaaatc gtctctaat cgcagtgtt gttccgttt 
      ggttgattt cgagggcag acttatcat tgatgactg 
      tgatacgaa agaattgag tttgccagc gttacgctc 
      caaaatttc aatcattcc gcaagaacc tatcctatt 
      ttcgggaac agtgcgtaa aaacttaga cccctttga 
      tcaatatca agacgacca aatatggag cgtactgga 
      gaaagtaaa attgaagga attcgttgc cagttctga 
      tctaggatt acacagcaa tttggcaga aggcggatc 
      aaattttag cgttggtca gaagcaact gatttgtct 
      ggctcgagc gttactgag agacagcaa aattttgat 
      tcttgatga agctaccgc taatgttga tccacacac 
      tgatgaact gttgcagaa gactattag ggaaaattt 
      cgaaaattg tacagttct cactattgc acatcggtt 
      acatactgt aatggattc cgacaaggt tcttgtaat 
      ggatgacgg aaaagcggt tgagtttga tcaccccca 
      cgcattgtt acaaaagaa gggagtttt ctttgattt 
      agtcatgga gacggggaa agcaatggg tgccaattt 
      gtcagaaat tgcccaaga gaattactt gaagaggca 
      gaatctttt agcacaata gtagacact agtacgtac 
      aaaaattca agtttctgt gatagatac gacctgatg 
      tacaaatag ttcgctctt caatgtgat atttattat 
      taaattatt taaaaattg
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