| General information | |||
| TC014089 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 5549980..5555647 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014089 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05554 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
- >TC014089 RA
MESKHAVKV DQLKKHPKE NSNLLSDLF YCWTFPMIK TGLKKALSE KDIYQTLTS HRSTNLTNK LERAWNSQK NGSNKPSLW RAIWKVFKW DIMLSIFLY FVTDIVLKI IRLIALGIL LGYYSPNAT EMSKSDAII CASVIVTSS FLLEILEHA YILHFQQLA MKVRVACAS LIYRKALKL SKKALSETT IGHIINLLS NDMQRLVAV WGAYHALWA APLQAALIL FLLYWIAGP TALVGNIFL LVLTPLQVF MTKKTADYR LKISLKSDE RIRYMSEII SGIQVIKVY TWELPFVKL IDAIRRKEM YFIKIANFL DSITIAFEF FFDRTSVFI CLVTYVLLG STPDAQYVF VLASFYDLM MQSVSLYFP QGLTITLQA NVAIKRFEN FLNLHEIQN KIITTKETG ITIDQVSAK WSETSQQNT LSDIKFNLE PKQLVAIIG PIGSGKSSL LQLCLGELA PNEGCVKIG GRISYANQE PWLFAGSVK QNILFGQAM VREKYQEVI RVCALEDDI AQFPYGDNT IVGERGILL SGGQKARIN LARAIYKDA DIYLLDDPL SAVDARVGK QIFNNCIMN YLKGKCTVL VTHQIQYLS FVDKIYLMV DGKVAVSGS YKELQASGE DFTRLLKEH EKYDESEDE SVVESKASK EDKEQDEEE ETWIQGKVS KKVYISYLR ASGNYFRFA VMGMLFIMT EILATGSDY FITFWVNLE QKRTAENKT KNETLSSDP VDSFFTNEN CIYVYTAII GALILFSIL RSMSFFQSC MKASVRLHD KMFTSVINA TMKFFYTNS SGRILNRFA KDMGSIDET LPEVFLDAL QMSMAIIGT VLAICIVSP WTLIPSIII VIIMLLLRL VYLSTTRDV KRMESTNRS PIFAHLTES MKGLTTIRA YNTQGILEK EFDTYQDVH TSIYYMYLG GNRALAVYL DIVCVLYVF CITVIALTK ETYAGNVGF MITQGMSMS GMFQWGIRQ WSEMENQMT SVERVGEYI DVEREKDTK TRDPPRQWP EHGKIEFKS VSMRYSSND PYVLKNLNI SITPREKVG IVGRTGAGK SSLIAVLFR LVDFEGRLI IDDCDTKEL SLPALRSKI SIIPQEPIL FSGTVRKNL DPFDQYQDD QIWSVLEKV KLKEFVASS DLGLHSNLA EGGSNFSVG QKQLICLAR ALLRDSKIL ILDEATANV DPHTDELLQ KTIRENFEN CTVLTIAHR LHTVMDSDK VLVMDDGKA VEFDHPHAL LQKKGVFFD LVMETGKAM GANLSEIAQ ENYLKRQNL LAQ
- >TC014089 RA
atggaatcg aaacatgcc gtcaaagtt gatcagtta aagaaacat cctaaggaa aactcgaat ctcttgtct gatctcttt tactgctgg acattccca atgattaag acaggttta aaaaaggca ttatcggag aaagatatt tatcaaact ctgaccagt catcggtcc acaaatttg acaaataaa ctagagcga gcatggaat agtcagaaa aatggaagc aataaaccc tctctttgg agagcaatt tggaaggtt tttaaatgg gacataatg ctttctata tttctttat ttcgtaaca gacattgtt ctcaaaatc atccgtctt atagctcta ggaatactg ttgggttac tactctcct aacgcaaca gaaatgagc aaaagcgac gcaattatt tgcgcttca gttatagta acttcgagt tttcttctc gaaatatta gaacatgcc tacatactt catttccaa caactggca atgaaagta cgcgtggcc tgtgcttct cttatttat cgaaaagct ctaaagctt agtaagaag gcattaagt gaaacgacc atagggcat attatcaat ttattgtca aatgacatg cagaggcta gtcgctgtt tggggagct tatcatgct ctttgggca gcgcctctt caggcagcc cttatcctt tttctactg tactggatc gcaggacca acggcttta gttggaaat atcttcctt ctagtactc acaccgttg caggtgttc atgactaaa aaaactgcc gattatcga ttaaaaata tctttaaaa agcgacgaa cgaatacgt tatatgagc gaaatcata tcaggaatt caagtaatc aaggtttac acctgggag ttacctttt gtcaaactt atagacgct attagaagg aaagaaatg tattttata aaaattgcc aacttctta gactcaatc acaatcgcc tttgaattc ttctttgat cgcacttct gtatttatt tgcctagtg acttacgtt ttgcttggg agtacccca gatgcccag tatgttttt gtccttgcc tcattctat gatttgatg atgcagtca gtttcacta tactttccc caagggttg acaataact cttcaagca aatgtggcc attaaaaga tttgaaaac ttcctcaac ttgcacgaa atacaaaac aagataatc acaactaaa gaaactgga attacgata gaccaagtt tccgcgaaa tggtctgaa acttcccaa caaaacact ttatcagac atcaaattt aacttggag ccaaaacaa ctagtcgcg ataattgga cccattggt agtgggaaa tccagtctc ttgcagtta tgtctaggt gagttggca cctaacgaa ggttgtgta aaaattggc ggtcgaata tcgtacgcc aaccaggag ccttggctg tttgccggt tctgtcaaa caaaacata cttttcggg caagcgatg gttagagag aagtaccaa gaagtgatt agagtttgt gctctggag gacgatatt gcccagttc ccgtacggc gataacaca atcgtaggc gaacgcgga attcttcta agtggtggt caaaaggcc agaataaac ctcgccagg gcaatttac aaagatgct gatatttat ttacttgac gatccttta tcggcagtg gacgcacgc gttggcaaa caaattttc aacaactgt attatgaat tatttaaaa ggcaaatgc acagttctt gttactcat caaattcag tacttaagt ttcgtcgat aagatatat ttgatggta gatggaaaa gttgcggtt tctggaagt tacaaagag ttgcaagcg tcaggtgaa gactttact agactttta aaagaacat gaaaaatac gacgaaagt gaggacgaa tccgttgtt gaaagtaaa gcaagtaaa gaggataaa gagcaagac gaagaagaa gagacttgg attcaagga aaagtgtcc aagaaagtg tacatttcg tatctccgt gcctctgga aattatttc aggtttgct gtaatggga atgttgttt atcatgacc gagattttg gccactggt tctgattac tttattact ttctgggtg aatttggaa caaaaacga acagcggaa aacaaaacc aaaaatgag actttatca tcagatcct gtagattct ttctttaca aatgaaaac tgtatttac gtttacaca gctataatt ggggccctg atattgttc tccattcta cgttcaatg tcttttttc caaagttgc atgaaagcg tctgtccgc ttgcacgac aaaatgttt actagcgta ataaatgcc actatgaaa tttttctac accaattct tctggaaga atactcaac agatttgcc aaagacatg gggagcata gacgagact ttacctgaa gtgtttttg gatgctctt cagatgtca atggcaatt attggcacg gtcttagcc atatgcatt gtgagtccc tggactctc atccccagt atcataatt gttataata atgttgctt cttagatta gtttacctt tctaccact agagacgtg aaacggatg gaatcaaca aaccggagt ccaattttt gcacattta actgagtca atgaagggt ttgacgaca ataagagcc tacaacacg caagggatc ttagagaag gagttcgat acttatcag gacgtacac acttctatc tattacatg tatttgggc ggcaacaga gccctggcc gtttatttg gacatagtt tgtgtcctt tatgttttc tgcatcacg gtcattgct ttgacaaaa gaaacctac gcgggaaac gtcggtttt atgataact cagggtatg agtatgagt ggtatgttt caatggggg atacgacag tggagcgaa atggagaat caaatgact tcggttgaa cgtgtcggg gaatacatc gatgttgaa cgtgaaaaa gacactaaa actagagac cccccacga cagtggcct gaacacggc aaaatcgaa ttcaagtct gtctccatg agatattcc tcaaacgat ccatacgta ctcaaaaat ctcaatatc tcgatcaca cctagagaa aaagtggga attgtgggt cgaactgga gccggaaaa tcgtctcta atcgcagtg ttgttccgt ttggttgat ttcgagggc agacttatc attgatgac tgtgatacg aaagaattg agtttgcca gcgttacgc tccaaaatt tcaatcatt ccgcaagaa cctatccta ttttcggga acagtgcgt aaaaactta gaccccttt gatcaatat caagacgac caaatatgg agcgtactg gagaaagta aaattgaag gaattcgtt gccagttct gatctagga ttacacagc aatttggca gaaggcgga tcaaatttt agcgttggt cagaagcaa ctgatttgt ctggctcga gcgttactg agagacagc aaaattttg attcttgat gaagctacc gctaatgtt gatccacac actgatgaa ctgttgcag aagactatt agggaaaat ttcgaaaat tgtacagtt ctcactatt gcacatcgg ttacatact gtaatggat tccgacaag gttcttgta atggatgac ggaaaagcg gttgagttt gatcacccc cacgcattg ttacaaaag aagggagtt ttctttgat ttagtcatg gagacgggg aaagcaatg ggtgccaat ttgtcagaa attgcccaa gagaattac ttgaagagg cagaatctt ttagcacaa tag
- >TC014089 RA
ttattcgaa atacactag ttatcagtt agttgtata gtttgcaac atttagtgt atgcagtat tagctcatc tacgcagct ttaagtgat ttccttgtt atttgatct catctaatt atttataat ggaatcgaa acatgccgt caaagttga tcagttaaa gaaacatcc taaggaaaa ctcgaatct cttgtctga tctctttta ctgctggac attcccaat gattaagac aggtttaaa aaaggcatt atcggagaa agatattta tcaaactct gaccagtca tcggtccac aaatttgac aaataaact agagcgagc atggaatag tcagaaaaa tggaagcaa taaaccctc tctttggag agcaatttg gaaggtttt taaatggga cataatgct ttctatatt tctttattt cgtaacaga cattgttct caaaatcat ccgtcttat agctctagg aatactgtt gggttacta ctctcctaa cgcaacaga aatgagcaa aagcgacgc aattatttg cgcttcagt tatagtaac ttcgagttt tcttctcga aatattaga acatgccta catacttca tttccaaca actggcaat gaaagtacg cgtggcctg tgcttctct tatttatcg aaaagctct aaagcttag taagaaggc attaagtga aacgaccat agggcatat tatcaattt attgtcaaa tgacatgca gaggctagt cgctgtttg gggagctta tcatgctct ttgggcagc gcctcttca ggcagccct tatcctttt tctactgta ctggatcgc aggaccaac ggctttagt tggaaatat cttccttct agtactcac accgttgca ggtgttcat gactaaaaa aactgccga ttatcgatt aaaaatatc tttaaaaag cgacgaacg aatacgtta tatgagcga aatcatatc aggaattca agtaatcaa ggtttacac ctgggagtt accttttgt caaacttat agacgctat tagaaggaa agaaatgta ttttataaa aattgccaa cttcttaga ctcaatcac aatcgcctt tgaattctt ctttgatcg cacttctgt atttatttg cctagtgac ttacgtttt gcttgggag taccccaga tgcccagta tgtttttgt ccttgcctc attctatga tttgatgat gcagtcagt ttcactata ctttcccca agggttgac aataactct tcaagcaaa tgtggccat taaaagatt tgaaaactt cctcaactt gcacgaaat acaaaacaa gataatcac aactaaaga aactggaat tacgataga ccaagtttc cgcgaaatg gtctgaaac ttcccaaca aaacacttt atcagacat caaatttaa cttggagcc aaaacaact agtcgcgat aattggacc cattggtag tgggaaatc cagtctctt gcagttatg tctaggtga gttggcacc taacgaagg ttgtgtaaa aattggcgg tcgaatatc gtacgccaa ccaggagcc ttggctgtt tgccggttc tgtcaaaca aaacatact tttcgggca agcgatggt tagagagaa gtaccaaga agtgattag agtttgtgc tctggagga cgatattgc ccagttccc gtacggcga taacacaat cgtaggcga acgcggaat tcttctaag tggtggtca aaaggccag aataaacct cgccagggc aatttacaa agatgctga tatttattt acttgacga tcctttatc ggcagtgga cgcacgcgt tggcaaaca aattttcaa caactgtat tatgaatta tttaaaagg caaatgcac agttcttgt tactcatca aattcagta cttaagttt cgtcgataa gatatattt gatggtaga tggaaaagt tgcggtttc tggaagtta caaagagtt gcaagcgtc aggtgaaga ctttactag acttttaaa agaacatga aaaatacga cgaaagtga ggacgaatc cgttgttga aagtaaagc aagtaaaga ggataaaga gcaagacga agaagaaga gacttggat tcaaggaaa agtgtccaa gaaagtgta catttcgta tctccgtgc ctctggaaa ttatttcag gtttgctgt aatgggaat gttgtttat catgaccga gattttggc cactggttc tgattactt tattacttt ctgggtgaa tttggaaca aaaacgaac agcggaaaa caaaaccaa aaatgagac tttatcatc agatcctgt agattcttt ctttacaaa tgaaaactg tatttacgt ttacacagc tataattgg ggccctgat attgttctc cattctacg ttcaatgtc ttttttcca aagttgcat gaaagcgtc tgtccgctt gcacgacaa aatgtttac tagcgtaat aaatgccac tatgaaatt tttctacac caattcttc tggaagaat actcaacag atttgccaa agacatggg gagcataga cgagacttt acctgaagt gtttttgga tgctcttca gatgtcaat ggcaattat tggcacggt cttagccat atgcattgt gagtccctg gactctcat ccccagtat cataattgt tataataat gttgcttct tagattagt ttacctttc taccactag agacgtgaa acggatgga atcaacaaa ccggagtcc aatttttgc acatttaac tgagtcaat gaagggttt gacgacaat aagagccta caacacgca agggatctt agagaagga gttcgatac ttatcagga cgtacacac ttctatcta ttacatgta tttgggcgg caacagagc cctggccgt ttatttgga catagtttg tgtccttta tgttttctg catcacggt cattgcttt gacaaaaga aacctacgc gggaaacgt cggttttat gataactca gggtatgag tatgagtgg tatgtttca atgggggat acgacagtg gagcgaaat ggagaatca aatgacttc ggttgaacg tgtcgggga atacatcga tgttgaacg tgaaaaaga cactaaaac tagagaccc cccacgaca gtggcctga acacggcaa aatcgaatt caagtctgt ctccatgag atattcctc aaacgatcc atacgtact caaaaatct caatatctc gatcacacc tagagaaaa agtgggaat tgtgggtcg aactggagc cggaaaatc gtctctaat cgcagtgtt gttccgttt ggttgattt cgagggcag acttatcat tgatgactg tgatacgaa agaattgag tttgccagc gttacgctc caaaatttc aatcattcc gcaagaacc tatcctatt ttcgggaac agtgcgtaa aaacttaga cccctttga tcaatatca agacgacca aatatggag cgtactgga gaaagtaaa attgaagga attcgttgc cagttctga tctaggatt acacagcaa tttggcaga aggcggatc aaattttag cgttggtca gaagcaact gatttgtct ggctcgagc gttactgag agacagcaa aattttgat tcttgatga agctaccgc taatgttga tccacacac tgatgaact gttgcagaa gactattag ggaaaattt cgaaaattg tacagttct cactattgc acatcggtt acatactgt aatggattc cgacaaggt tcttgtaat ggatgacgg aaaagcggt tgagtttga tcaccccca cgcattgtt acaaaagaa gggagtttt ctttgattt agtcatgga gacggggaa agcaatggg tgccaattt gtcagaaat tgcccaaga gaattactt gaagaggca gaatctttt agcacaata gtagacact agtacgtac aaaaattca agtttctgt gatagatac gacctgatg tacaaatag ttcgctctt caatgtgat atttattat taaattatt taaaaattg
