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Gene information
General information
TC014085 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5530540..5532632
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014085
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05553

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014085 RA
      METNDGVKS QKTYLQHLS NVIKRVTFG ILMISGFLI 
      IILKGPVML MLLAFLIQV FCFKEILKI GYDSNKLPH 
      NLPYFRFLC WYFFMVANY FFSIETIAP YFQSYCNKF 
      YNLKIMISY HRFISFCAY FGGIVLFVL SLVRKYDLK 
      QFRILAWTH TLLILIVLQ SYMIIGNMF NGLIWLILP 
      VSLVILNDI FAYVFGRLC GKTPLIKLS PKKTWEGFI 
      GGVVGTFIS GFFLSNIMC RYKYFVCPI EYYETGNSV 
      ALSSDCTPS YLFLPKKYF LWFFSVDLY PFTYHFVAL 
      ALFASIIAP FGGFCASGF KRAFKLKDF GDVIPGHGG 
      IMDRFDCQF LMATFVNVY IFTFVKSPS VELIFQKIS 
      YLDEKKQLQ FYYLLREFL QANDLGNST YFDL
    CDS sequence
    • >TC014085 RA
      atggagacc aacgatggc gtgaagtcg caaaagacg 
      tatttacag cacttgagc aacgtgatt aaacgtgtg 
      actttcggt atactcatg atttccgga ttccttatc 
      ataattctc aaaggccca gttatgcta atgttacta 
      gcttttcta atccaagtt ttctgtttc aaagagatt 
      cttaaaatt ggttacgac agtaacaaa cttccacac 
      aacttaccc tattttcga tttctgtgt tggtatttt 
      ttcatggtg gcgaactat ttcttttcg attgaaacg 
      atagcaccg tattttcaa agttactgt aataaattt 
      tataattta aaaataatg ataagttat cataggttt 
      atttcgttt tgtgcctat ttcggagga attgtcctt 
      ttcgtgtta agtttggtc aggaaatat gatctgaag 
      cagtttcgg attcttgct tggactcac acactgttg 
      atactcatc gttcttcag tcgtacatg attataggc 
      aatatgttt aacggtttg atttggttg attcttcca 
      gtttccttg gttatttta aacgatatt ttcgcatac 
      gtattcggg cgactgtgt gggaaaacg cccttaata 
      aaactatcg ccgaaaaag acgtgggaa ggttttatt 
      gggggcgtt gtaggaaca ttcattagt ggcttcttt 
      ttatcaaat attatgtgc cggtacaag tattttgtt 
      tgtccgatc gagtattac gaaactggt aatagtgtt 
      gccttatcg tccgattgc actcccagt tatttattt 
      cttccgaaa aagtatttt ttgtggttc ttttctgta 
      gatctttat cctttcacg tatcacttt gtggcactt 
      gccctattt gcgagcata atcgctccg tttggagga 
      ttttgcgcg tcgggcttt aagcgagct ttcaaattg 
      aaagacttc ggtgacgtg attcccgga catggagga 
      attatggac agatttgat tgccagttc ttaatggcc 
      acttttgtg aacgtctac atttttacg ttcgtgaaa 
      agtccgagt gttgagctc attttccaa aaaatatcg 
      tatttggat gagaaaaaa cagctgcag ttttattac 
      ctcttgaga gagtttctg caagcaaat gacttgggg 
      aattctacg tactttgat ttgtaa
    mRNA sequence
    • >TC014085 RA
      gttaattca ttttataac tagtaacta cgatgcaat 
      ttgtaaata tttgtttgt gtcaaggtc aggcttcct 
      caaggatgt tttaattag gtatttagc agcggtggc 
      acaatacat cgcactaaa tccagcaca cataggatg 
      gagaccaac gatggcgtg aagtcgcaa aagacgtat 
      ttacagcac ttgagcaac gtgattaaa cgtgtgact 
      ttcggtata ctcatgatt tccggattc cttatcata 
      attctcaaa ggcccagtt atgctaatg ttactagct 
      tttctaatc caagttttc tgtttcaaa gagattctt 
      aaaattggt tacgacagt aacaaactt ccacacaac 
      ttaccctat tttcgattt ctgtgttgg tattttttc 
      atggtggcg aactatttc ttttcgatt gaaacgata 
      gcaccgtat tttcaaagt tactgtaat aaattttat 
      aatttaaaa ataatgata agttatcat aggtttatt 
      tcgttttgt gcctatttc ggaggaatt gtccttttc 
      gtgttaagt ttggtcagg aaatatgat ctgaagcag 
      tttcggatt cttgcttgg actcacaca ctgttgata 
      ctcatcgtt cttcagtcg tacatgatt ataggcaat 
      atgtttaac ggtttgatt tggttgatt cttccagtt 
      tccttggtt attttaaac gatattttc gcatacgta 
      ttcgggcga ctgtgtggg aaaacgccc ttaataaaa 
      ctatcgccg aaaaagacg tgggaaggt tttattggg 
      ggcgttgta ggaacattc attagtggc ttcttttta 
      tcaaatatt atgtgccgg tacaagtat tttgtttgt 
      ccgatcgag tattacgaa actggtaat agtgttgcc 
      ttatcgtcc gattgcact cccagttat ttatttctt 
      ccgaaaaag tattttttg tggttcttt tctgtagat 
      ctttatcct ttcacgtat cactttgtg gcacttgcc 
      ctatttgcg agcataatc gctccgttt ggaggattt 
      tgcgcgtcg ggctttaag cgagctttc aaattgaaa 
      gacttcggt gacgtgatt cccggacat ggaggaatt 
      atggacaga tttgattgc cagttctta atggccact 
      tttgtgaac gtctacatt tttacgttc gtgaaaagt 
      ccgagtgtt gagctcatt ttccaaaaa atatcgtat 
      ttggatgag aaaaaacag ctgcagttt tattacctc 
      ttgagagag tttctgcaa gcaaatgac ttggggaat 
      tctacgtac tttgatttg taatttact tcaagtctg 
      cgaaataaa tcttacgtt ttttatgtt gttttattt 
      atgaaaaag tcaacgatt cctctacgg caatttgtt 
      gggaggttg taggagtaa gctccgcct ttgattgtg 
      cgatgcccg cctctcatc taagatcca gattcggtt 
      tattatcac aatgctgga catcgccgg atactttga 
      aggtgcagc aagtaaatc acttgaaag gcatcgaga 
      atattgtac ttgtattgg aaataagaa ataaagttt 
      ttgtaaccg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY