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Gene information
General information
TC014084 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5504722..5512958
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014084
    iBeetle screen iB_05552

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 5

    maxillary palp thinner - Number of animals showing the phenotype: 6
    labial palp thinner - Number of animals showing the phenotype: 6
    labial palp elongated - Number of animals showing the phenotype: 6
    maxillary palp elongated - Number of animals showing the phenotype: 6
    wings not closed - Number of animals showing the phenotype: 6

    wings orientation irregular - Number of animals showing the phenotype: 3
    legs random not present - Number of animals showing the phenotype: 2
    legs tarsus shape irregular - Number of animals showing the phenotype: 5

    female genital lobes mostly not present - Number of animals showing the phenotype: 6

    Adult morphology
    adult appendages irregular - Number of animals showing the phenotype: 2


    antenna distal colour lighter - Number of animals showing the phenotype: 2


    antenna distal bent - Number of animals showing the phenotype: 2

    maxillary palp thinner - Number of animals showing the phenotype: 2


    maxillary palp partially not present - Number of animals showing the phenotype: 2

    labial palp thinner - Number of animals showing the phenotype: 2

    legs tarsomere partially not present - Number of animals showing the phenotype: 2

    legs tarsomere sometimes distalcuticularisation decreased - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014084 RA
      MKVTVIFVM NLFVLTALG DVTNARSKN RGKGSMWWG 
      IAKAGEPNN LSPISPGGV YMDLSSAHG TLRRKQRRL 
      ARENPGLLV ALHKGANNA IHECQHQFR NQRWNCSTR 
      AFPRGKNLF GKIVDKGCR ETAFIYAIT SAAVTHAIA 
      RACSEGSID TCNCETHYK GRPHVSGNG GGAAVAGVR 
      DFEWGGCSD NIGFGFTVS REFVDAGER GKTIREKMN 
      LHNNEAGRW HVKDQMRQE CKCHGMSGS CTIKTCWMR 
      LPPFRVIGD LLKDRFDGA SHVAASGHH RNNNNAHQN 
      RPPKNPKLN AISSNSIHS KRENRRKHK YGFQLKPFN 
      PEHKPPGTK DLVYYEMSP GFCEKNPKL GIQGTHGRL 
      CNDTSMGVD GCDIMCCGR GYRTQEVVV FERCNCTFH 
      WCCEVKCDV CRTKRTIHT CV
    CDS sequence
    • >TC014084 RA
      atgaaggtg acagtgata ttcgtgatg aatctcttt 
      gtgctcacc gctctcggg gatgttacc aacgccaga 
      agcaagaac cgcggcaag ggctccatg tggtggggc 
      attgcaaaa gccggcgag ccgaacaat ctatcgcca 
      atatcaccc ggaggtgtc tacatggac ctttcgtcg 
      gcccatggc accctgcga cggaagcag cgtcgcctg 
      gctcgggaa aatcccggc ttgttggtg gctctacat 
      aaaggtgcc aataatgcg attcacgag tgccagcat 
      cagtttcgc aatcagcgc tggaactgt tcgacgaga 
      gccttccct cgaggaaag aacttgttc ggaaaaata 
      gtcgataaa ggatgcagg gaaactgcc ttcatttat 
      gcgataacg agtgctgcg gtgacccac gcgatcgcc 
      agagcttgc agcgaaggt tcgatagat acgtgcaac 
      tgcgaaacg cactacaaa ggaaggccg catgtttct 
      gggaacggc ggcggggcc gccgttgcc ggcgtcaga 
      gatttcgaa tggggcggc tgttcggat aatattggg 
      ttcggattc acggtcagt cgggagttt gtcgacgcc 
      ggcgaaagg ggcaaaacc attcgagaa aagatgaat 
      ttgcacaac aacgaggcc ggaagatgg cacgtaaag 
      gatcagatg cgtcaagaa tgtaaatgc cacggaatg 
      tccggttct tgcactatt aagacttgt tggatgcga 
      cttcctccg tttcgagtg atcggcgac ctcctcaaa 
      gaccgcttc gacggcgct tctcatgtc gccgcctcc 
      ggccaccac cgaaacaac aacaacgcg caccagaac 
      cgtccaccg aagaacccc aagctgaac gcgatctcc 
      agtaacagc atccacagt aagagggag aatcgccgc 
      aagcacaag tacggcttc cagttgaaa ccgttcaac 
      cccgagcac aaacccccc ggaaccaag gatctggtc 
      tactacgag atgtcgccc ggcttctgc gagaagaac 
      ccgaagctg gggattcaa gggacgcat gggcggctg 
      tgcaacgac acctcgatg ggtgtcgat gggtgcgat 
      atcatgtgc tgcggacgg gggtatcgt acccaggaa 
      gtcgtcgta ttcgagaga tgcaactgt acgtttcat 
      tggtgctgt gaagtgaag tgcgatgtg tgtcgaacg 
      aaaagaacc atacatact tgcgtttaa
    mRNA sequence
    • >TC014084 RA
      tcagtgcca caaagaact ctgctgtgg gtggtcgga 
      acaaaaatt gactcctcc taaggtaac aactcgtac 
      ttggtcggt tatgttcgg acacaataa ccaccccta 
      ctctaccct ctcgagctg agtaaatag tcagataca 
      ggtgatatt gtttatttt ttgcgttcg tgtgtgcgg 
      gtggacact atgaaggtg acagtgata ttcgtgatg 
      aatctcttt gtgctcacc gctctcggg gatgttacc 
      aacgccaga agcaagaac cgcggcaag ggctccatg 
      tggtggggc attgcaaaa gccggcgag ccgaacaat 
      ctatcgcca atatcaccc ggaggtgtc tacatggac 
      ctttcgtcg gcccatggc accctgcga cggaagcag 
      cgtcgcctg gctcgggaa aatcccggc ttgttggtg 
      gctctacat aaaggtgcc aataatgcg attcacgag 
      tgccagcat cagtttcgc aatcagcgc tggaactgt 
      tcgacgaga gccttccct cgaggaaag aacttgttc 
      ggaaaaata gtcgataaa ggatgcagg gaaactgcc 
      ttcatttat gcgataacg agtgctgcg gtgacccac 
      gcgatcgcc agagcttgc agcgaaggt tcgatagat 
      acgtgcaac tgcgaaacg cactacaaa ggaaggccg 
      catgtttct gggaacggc ggcggggcc gccgttgcc 
      ggcgtcaga gatttcgaa tggggcggc tgttcggat 
      aatattggg ttcggattc acggtcagt cgggagttt 
      gtcgacgcc ggcgaaagg ggcaaaacc attcgagaa 
      aagatgaat ttgcacaac aacgaggcc ggaagatgg 
      cacgtaaag gatcagatg cgtcaagaa tgtaaatgc 
      cacggaatg tccggttct tgcactatt aagacttgt 
      tggatgcga cttcctccg tttcgagtg atcggcgac 
      ctcctcaaa gaccgcttc gacggcgct tctcatgtc 
      gccgcctcc ggccaccac cgaaacaac aacaacgcg 
      caccagaac cgtccaccg aagaacccc aagctgaac 
      gcgatctcc agtaacagc atccacagt aagagggag 
      aatcgccgc aagcacaag tacggcttc cagttgaaa 
      ccgttcaac cccgagcac aaacccccc ggaaccaag 
      gatctggtc tactacgag atgtcgccc ggcttctgc 
      gagaagaac ccgaagctg gggattcaa gggacgcat 
      gggcggctg tgcaacgac acctcgatg ggtgtcgat 
      gggtgcgat atcatgtgc tgcggacgg gggtatcgt 
      acccaggaa gtcgtcgta ttcgagaga tgcaactgt 
      acgtttcat tggtgctgt gaagtgaag tgcgatgtg 
      tgtcgaacg aaaagaacc atacatact tgcgtttaa 
      agcatgctc gtacttaga tatttgcca attttacgc 
      tttattcac ttggaacta aagttttat ttattttta 
      cgtattcgt attctccag tattactta aaattgata 
      gggatttga aattgattt gatgtgaat aaagctagg 
      ggagcccgg tgggagtta acacggcga cagtattaa 
      ctcattaca ggccgaacg cttattatc agtattaga 
      aattattat tgacattat caatgtaaa agactgatt 
      tgaaatttg ttttgagtc atatttgta catattgcg 
      ctaagttat attctacgc tacaagcat tagttttac 
      gtggcgggc tggccacgc catttttta ttttttctg 
      tattaggta ggtacgtac atatttatt tatggaatg 
      taaatacaa aaaatcagc atgtagtgt atctcaaat 
      tgtgtgaat atgtgacca ctttaggac ttatacaga 
      tcgttctat aatcgcatt aatttattg accattaac 
      tgttgtaca ttagcattt gtaaatatt catgtagct 
      tatttatgt agataataa ttagcgagt atttttcta 
      attacttta tttataatt aaacgtact tgtaaatct 
      ctcgattgc tataaaatc attaataaa caattatat 
      gtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY