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Gene information
General information
TC014075 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5376890..5382661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014075
    iBeetle screen iB_05550

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014075 RA
      MDFDDPALL KKPIKEIEE KWKLVPAFL KVKGLVKQH 
      IDSFNYFIN VDIKKIVEA NNKLVCDAD PLFYVKYTN 
      VYVGRPDVD EGFNISKPT TPHECRLRD MTYSAPISV 
      DIEYTRGTQ RVSKAGLMI GRMPIMLRS SNCVLTNKS 
      EYELAKMNE CPLDPGGYF VVKGQEKVI LIQEQLSKN 
      RMIVEESKF GVQCAVTSS THEKKSRTN VVCKANKYY 
      LGHNSLTDL VPVSIIFKA LDVTSDKEI CELIGVEDI 
      SKLTPTLEE CHTLKIYTQ LGALKHIGS KLVAKRYVT 
      SASKMKTPV DEARDVLAT TVLAHVPVE NYNFRMKAI 
      YLGVMVKRV IEAQSDKKF MDDKDYYGN KRLELAGSL 
      LSLMFEDVF KRFNWELKS IAEKTIPKI RATQFDVVK 
      YMRSDLITN CLVYAISTG NWTIKRFRM ERLGVTQVL 
      SRLSYISAL GMMTRVNSQ FEKTRKVSG PRSLQPSQW 
      GMLCPSDTP EGEACGLVK NLALMTHIT TDVDEYPLI 
      RLANNAGVV DINMSSGMS IHSPETFFV FLNGNILGT 
      IRNYKKLIH TFRMLRRSG LISGYVSIY PNFLHRCIY 
      ISSDGGRLC RPYIIVERG RPLVTQNHI IELEKGIRS 
      FEDFLHDGL IEFLDVNEE NDSFIACYE KDITDLTTH 
      LEIATFTLL GVCAGLVPY PHHNQSPRN TYQCAMGKQ 
      AMGTIGYNQ KNRMDTLLY SLVYPQAPM VKTKSIELT 
      NFDKLPAGQ NATIAVMSY SGYDIEDAL IINKASIDR 
      GFGRCLVYK NSKCVMKRY ANQTFDRIQ GPLIDTTTN 
      APMWKHAVL DMDGIVAPG ESVDNKQVL VNKSVPTVS 
      AVPGSQTTS VEYKDTPIC YRNNEPSYI EKVLVSTNE 
      EDTTIIKIL LRQTRRPEI GDKFSSRHG QKGVVGLVV 
      EQEDMPFNE YGICPDIIM NPHGYPSRM TVGKLIELL 
      AGKAGLVEG KFHYGTAFG GSKVNDVCE ELAKHGFNY 
      QGKDFFYSG ITGEPLEAY IYSGPVYYQ KLKHMVQDK 
      IHARGRGPR AVLTRQPTE GRSRDGGLR LGEMERDCL 
      IGYGASMML IERLMVSSD QCEVDVCNL CGRLGYCGW 
      CNSCKSSRE VSSITMPYA CKLLLTELQ AMNITARLT 
      LNHYCE
    CDS sequence
    • >TC014075 RA
      atggatttt gacgatccc gcccttctc aaaaaacca 
      atcaaagaa atcgaagaa aaatggaaa ctagtgccc 
      gccttttta aaagtcaaa ggattagta aagcaacac 
      attgattcg ttcaattac ttcatcaac gttgatatt 
      aagaaaatc gtggaagcc aacaacaaa ctagtttgt 
      gatgccgat cctttattc tacgtcaaa tatacaaat 
      gtttatgtt ggacgaccg gacgttgac gaaggcttc 
      aacattagc aaacctaca acccctcat gaatgtagg 
      ctgagggac atgacgtac tcagccccc atttccgta 
      gatattgag tacactaga ggcactcag cgggtatcc 
      aaagctggt ttaatgatt ggcagaatg ccaattatg 
      ctccgttcc tccaactgc gtcctcacc aacaaatcc 
      gagtacgaa ttggcaaaa atgaacgaa tgtccttta 
      gaccccggc ggctatttc gtcgtcaaa ggccaagaa 
      aaagtgatc ttaatccaa gaacagctc tccaaaaat 
      cgaatgata gttgaagag tccaaattt ggggtccaa 
      tgcgctgtc accagctcc acacatgag aagaagtcg 
      cgaaccaac gtcgtatgc aaggccaac aagtactac 
      ctaggacat aactcccta actgactta gtccctgtt 
      tcaatcatc ttcaaagct ttggatgtg acaagtgat 
      aaagaaatc tgcgaattg atcggagtt gaggatatt 
      tctaaactc actccaacg ttagaggag tgccacact 
      ttaaaaatt tacactcag ctgggggca cttaaacac 
      ataggttcg aaattagta gctaaacgt tacgtgacg 
      tctgctagc aaaatgaaa acgccagtt gatgaagcg 
      agggacgtt ttggcgacc actgttcta gcccacgtg 
      cctgtggaa aactacaat ttcaggatg aaggccatt 
      tatttggga gtgatggtc aaaagggtg attgaggca 
      cagtcggac aagaaattc atggatgac aaagactac 
      tacggcaac aagcgtctc gaattggcc ggctctctc 
      ctttctctt atgttcgag gacgttttc aaacgcttt 
      aactgggaa ctaaaatcc atcgctgaa aaaaccatc 
      ccaaaaatc cgagcgact caattcgac gttgtcaag 
      tatatgcgc tcggatctg atcaccaat tgtctggtc 
      tacgcaatt tcgacgggc aattggacc atcaagcga 
      ttccgaatg gagcgtttg ggtgtgact caggttttg 
      tcacggttg agctacatt tccgctttg gggatgatg 
      accagagtt aattcgcag tttgagaaa acgaggaag 
      gtttcagga cccaggtct ctgcagccg agtcagtgg 
      ggaatgctg tgtccgagc gatacaccc gaaggagag 
      gcgtgcgga ttggtgaaa aacctggcc ttgatgacc 
      cacatcacc accgatgtg gacgaatac cccctcatc 
      cgattggct aacaacgct ggagttgtt gacattaac 
      atgtcctca ggcatgtcc atccactcc cctgaaacg 
      ttttttgtt ttcttgaac ggtaacatc ctcggaacg 
      attcgcaac tacaagaaa ctgatccac acattccgg 
      atgttgcga cgctcaggc ctcatttcc ggttacgtt 
      tccatctac cccaacttt ttgcatcgc tgtatttac 
      attagttcg gacggaggg cgcttgtgc cgaccgtac 
      atcatagtc gagcgcgga cgccctctg gtcacgcaa 
      aaccacatc attgagctc gagaagggt attaggagc 
      tttgaggat tttttgcac gacggtttg atcgagttc 
      ctggatgtt aatgaggaa aatgatagt tttatcgct 
      tgttatgag aaagatatc actgatttg acgacgcat 
      ttggagatt gctacgttc actttgttg ggggtttgt 
      gccgggttg gtgccctac ccacatcat aaccaatca 
      cccaggaac acgtatcag tgtgctatg ggcaagcaa 
      gcgatgggt acgatcggt tataaccaa aaaaaccga 
      atggacacc ctcttgtat agtctcgtg tacccacaa 
      gccccaatg gttaaaaca aaatcgatc gagttgacc 
      aactttgat aagctccca gccggtcaa aatgccacg 
      atagctgtg atgagctac agcggttac gacatagag 
      gacgctctt atcataaat aaggcctcg attgataga 
      ggctttggc cgctgtctt gtgtacaaa aactcgaag 
      tgtgtgatg aaacgctac gccaatcag acttttgac 
      cgcattcag ggccctttg attgacacc accaccaac 
      gcccccatg tggaagcac gccgttctt gacatggac 
      ggaatagtg gcccctgga gagtcagtc gataacaag 
      caagtgcta gtcaataag tcagtaccg acggtttcg 
      gccgttccc ggcagccaa acgacctca gtcgagtac 
      aaagacacg cccatttgc taccgcaac aacgaaccg 
      agttatatc gaaaaagtg ctagtcagt acgaacgaa 
      gaggacacc acaataatc aaaattttg ttgagacaa 
      acacgtcgc cccgaaatc ggggacaaa ttcagttcg 
      agacatgga cagaagggg gttgtgggc ctggtggtg 
      gagcaggaa gacatgccg ttcaatgag tacgggatt 
      tgcccggat attatcatg aaccctcac ggatatccc 
      tcgcggatg accgtcggg aaattgatc gagctgttg 
      gccgggaaa gcgggtcta gttgaggga aaattccac 
      tacgggact gctttcggc ggctccaaa gtcaatgac 
      gtttgtgag gaattggcc aagcacggg tttaactac 
      caagggaag gatttcttc tattcgggg atcacaggg 
      gagcccctt gaggcctac atttactcc gggcctgtc 
      tattaccaa aaattgaaa catatggtt caggataaa 
      atacacgcg agggggcgt gggcctcgc gctgtgtta 
      acgagacaa ccaaccgaa gggcggagt agggatggg 
      gggctacgg ctcggtgaa atggaacgt gattgtctt 
      ataggatat ggggctagt atgatgttg atagaacgg 
      ttgatggtg tcaagtgac cagtgtgaa gttgacgtt 
      tgtaatctc tgtggccga ctgggatac tgtggatgg 
      tgtaatagt tgtaaaagt tcaagagaa gtttcaagt 
      attacaatg ccttatgct tgtaaattg cttttgact 
      gaattgcaa gcgatgaat atcacagca aggcttaca 
      ctgaatcat tattgtgaa taa
    mRNA sequence
    • >TC014075 RA
      acctacatg tttaacttc acattttca acatgtaaa 
      caaatagtt attaagcgt aacagtgat aaactaatt 
      aataagttg caacaaaaa gcgctgaaa tggattttg 
      acgatcccg cccttctca aaaaaccaa tcaaagaaa 
      tcgaagaaa aatggaaac tagtgcccg cctttttaa 
      aagtcaaag gattagtaa agcaacaca ttgattcgt 
      tcaattact tcatcaacg ttgatatta agaaaatcg 
      tggaagcca acaacaaac tagtttgtg atgccgatc 
      ctttattct acgtcaaat atacaaatg tttatgttg 
      gacgaccgg acgttgacg aaggcttca acattagca 
      aacctacaa cccctcatg aatgtaggc tgagggaca 
      tgacgtact cagccccca tttccgtag atattgagt 
      acactagag gcactcagc gggtatcca aagctggtt 
      taatgattg gcagaatgc caattatgc tccgttcct 
      ccaactgcg tcctcacca acaaatccg agtacgaat 
      tggcaaaaa tgaacgaat gtcctttag accccggcg 
      gctatttcg tcgtcaaag gccaagaaa aagtgatct 
      taatccaag aacagctct ccaaaaatc gaatgatag 
      ttgaagagt ccaaatttg gggtccaat gcgctgtca 
      ccagctcca cacatgaga agaagtcgc gaaccaacg 
      tcgtatgca aggccaaca agtactacc taggacata 
      actccctaa ctgacttag tccctgttt caatcatct 
      tcaaagctt tggatgtga caagtgata aagaaatct 
      gcgaattga tcggagttg aggatattt ctaaactca 
      ctccaacgt tagaggagt gccacactt taaaaattt 
      acactcagc tgggggcac ttaaacaca taggttcga 
      aattagtag ctaaacgtt acgtgacgt ctgctagca 
      aaatgaaaa cgccagttg atgaagcga gggacgttt 
      tggcgacca ctgttctag cccacgtgc ctgtggaaa 
      actacaatt tcaggatga aggccattt atttgggag 
      tgatggtca aaagggtga ttgaggcac agtcggaca 
      agaaattca tggatgaca aagactact acggcaaca 
      agcgtctcg aattggccg gctctctcc tttctctta 
      tgttcgagg acgttttca aacgcttta actgggaac 
      taaaatcca tcgctgaaa aaaccatcc caaaaatcc 
      gagcgactc aattcgacg ttgtcaagt atatgcgct 
      cggatctga tcaccaatt gtctggtct acgcaattt 
      cgacgggca attggacca tcaagcgat tccgaatgg 
      agcgtttgg gtgtgactc aggttttgt cacggttga 
      gctacattt ccgctttgg ggatgatga ccagagtta 
      attcgcagt ttgagaaaa cgaggaagg tttcaggac 
      ccaggtctc tgcagccga gtcagtggg gaatgctgt 
      gtccgagcg atacacccg aaggagagg cgtgcggat 
      tggtgaaaa acctggcct tgatgaccc acatcacca 
      ccgatgtgg acgaatacc ccctcatcc gattggcta 
      acaacgctg gagttgttg acattaaca tgtcctcag 
      gcatgtcca tccactccc ctgaaacgt tttttgttt 
      tcttgaacg gtaacatcc tcggaacga ttcgcaact 
      acaagaaac tgatccaca cattccgga tgttgcgac 
      gctcaggcc tcatttccg gttacgttt ccatctacc 
      ccaactttt tgcatcgct gtatttaca ttagttcgg 
      acggagggc gcttgtgcc gaccgtaca tcatagtcg 
      agcgcggac gccctctgg tcacgcaaa accacatca 
      ttgagctcg agaagggta ttaggagct ttgaggatt 
      ttttgcacg acggtttga tcgagttcc tggatgtta 
      atgaggaaa atgatagtt ttatcgctt gttatgaga 
      aagatatca ctgatttga cgacgcatt tggagattg 
      ctacgttca ctttgttgg gggtttgtg ccgggttgg 
      tgccctacc cacatcata accaatcac ccaggaaca 
      cgtatcagt gtgctatgg gcaagcaag cgatgggta 
      cgatcggtt ataaccaaa aaaaccgaa tggacaccc 
      tcttgtata gtctcgtgt acccacaag ccccaatgg 
      ttaaaacaa aatcgatcg agttgacca actttgata 
      agctcccag ccggtcaaa atgccacga tagctgtga 
      tgagctaca gcggttacg acatagagg acgctctta 
      tcataaata aggcctcga ttgatagag gctttggcc 
      gctgtcttg tgtacaaaa actcgaagt gtgtgatga 
      aacgctacg ccaatcaga cttttgacc gcattcagg 
      gccctttga ttgacacca ccaccaacg cccccatgt 
      ggaagcacg ccgttcttg acatggacg gaatagtgg 
      cccctggag agtcagtcg ataacaagc aagtgctag 
      tcaataagt cagtaccga cggtttcgg ccgttcccg 
      gcagccaaa cgacctcag tcgagtaca aagacacgc 
      ccatttgct accgcaaca acgaaccga gttatatcg 
      aaaaagtgc tagtcagta cgaacgaag aggacacca 
      caataatca aaattttgt tgagacaaa cacgtcgcc 
      ccgaaatcg gggacaaat tcagttcga gacatggac 
      agaaggggg ttgtgggcc tggtggtgg agcaggaag 
      acatgccgt tcaatgagt acgggattt gcccggata 
      ttatcatga accctcacg gatatccct cgcggatga 
      ccgtcggga aattgatcg agctgttgg ccgggaaag 
      cgggtctag ttgagggaa aattccact acgggactg 
      ctttcggcg gctccaaag tcaatgacg tttgtgagg 
      aattggcca agcacgggt ttaactacc aagggaagg 
      atttcttct attcgggga tcacagggg agccccttg 
      aggcctaca tttactccg ggcctgtct attaccaaa 
      aattgaaac atatggttc aggataaaa tacacgcga 
      gggggcgtg ggcctcgcg ctgtgttaa cgagacaac 
      caaccgaag ggcggagta gggatgggg ggctacggc 
      tcggtgaaa tggaacgtg attgtctta taggatatg 
      gggctagta tgatgttga tagaacggt tgatggtgt 
      caagtgacc agtgtgaag ttgacgttt gtaatctct 
      gtggccgac tgggatact gtggatggt gtaatagtt 
      gtaaaagtt caagagaag tttcaagta ttacaatgc 
      cttatgctt gtaaattgc ttttgactg aattgcaag 
      cgatgaata tcacagcaa ggcttacac tgaatcatt 
      attgtgaat aattgattt atgaaataa acataattt 
      tttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY