Status
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Gene information
General information
TC014056 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5010448..5016431
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014056
    iBeetle screen iB_05548

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014056 RA
      MESLNTNLL SQSLNFNGQ QMQKLWEAE YGENDLHRR 
      NVKDVNFQV YSERQKHLS FQDRGKRLK LQQFVVKKA 
      NMLFATEAS DFEYKPDEG PVSEDTYAI MPPFETFLN 
      VDKQQRLKY FFKSVKVGD LIIGTIVSK TQSGMMLKV 
      LCTAGPGPN IIYAADINV KAFCPVANI IPAVDKKGI 
      TRSYMMNDS VCCEVIEKI PDSDKMVCC MKGTPRKPN 
      DPERRPPLG LISADDFPL AYKKALDHK NENYDTVLE 
      KSIGFNNPN NISYLAGVV GIKDHCTLM KGLKGRFAE 
      NEYANELRQ VQASKWAYR NVADGIDHF KAGKHVEAF 
      QCLNKALNI DPKNVEGLV ARGALYANS GNFQKAIAD 
      FETALKLNP NHGNARKYM GETLVALGR SYEEEDKIE 
      EARKSYQSC LSLIPNHEE AQNSLKFLK NKTSATKNL 
      IEPTELLLP NLSTASKTA DVNDALKQL LKNEDEEKK 
      DKKKKKKSK KRKNRRHSS SSSSSSSSG SNDSSSSSS 
      SSSSDSSSS SGDSDFKKR KKHRSRSHR REKQSSLSP 
      LSKRMAMMD QSHDTPNTY TFNKPAATS AMFDFNLEQ 
      PSETQKPAD LSYEQRLRM FLQETKGDS DYEEKVRKF 
      LEESSKWKK EKRSDEKKK KRKKDRKNK KDSKKKKKE 
      KKLKRKGFD LSEFEDHKK LRDAIKKEL SKGKKSKKH 
      SIPSDDDEY FLQKAGYTK RFIDSLPDL EELESKLNA 
      YYAMEKESK RSELSESPK RGLLDSPSP TREQKARQA 
      VAEAAATAG SANKWKMQV ASQPPPSTS GSRFKKKPD 
      REEWKEEQQ QQQMMDKRG GGGGGGMAS GGKNNNFEE 
      SVRKNQQAP VPPPEKNMS VRKAMAMKE PPPPKRPDS 
      PKNKPKLLP PIPAVNPLV NPGQVVLDK FGNFRLMTP 
      PELKKGRDD MPPLPPGRR PPEPPGRPR SDSRSPKRH 
      SRSRSRRTR SSSASRSRS RSYRSRSRS YYSRSRSRS 
      GSYYSRSRS RSRSYSGDR RRYRRGGFR GRYNDRGTY 
      YKPRFQNPR NNIRGRGGG GGGGGYYNR DGRYNDREY 
      RGRGRPYNN YNNRGGRRP RGRYQRGGY REYRDRRDY 
      RSRSRDRSR SMSGSRDRD SDEAMKKAA NAEKDNKVN 
      KYADNEGEQ IRHEGPLSE GEDRDDYTS NEKFVDREA 
      FGGKWAEKE EGGGQDNGG GGDGDGAKV DKTNIPSES 
      NIEEMDKFL TKVKKDKKE EMLERNKDL LKNKS
    CDS sequence
    • >TC014056 RA
      atggagtcg ttgaacacg aaccttctg agccagtcg 
      ctgaatttc aatggacag caaatgcaa aagttgtgg 
      gaggctgaa tacggcgag aatgatttg caccgtagg 
      aacgtcaag gatgttaac ttccaagtg tacagcgag 
      agacagaag catttgtct ttccaggat cgaggaaaa 
      cgactcaaa ctccagcag tttgtcgtc aagaaagca 
      aacatgttg tttgcgact gaagcttcc gattttgag 
      tataaacct gacgagggg cccgttagt gaggacacg 
      tacgcgatc atgccccca tttgagacg tttcttaat 
      gttgacaag cagcaaagg ctcaaatat ttctttaag 
      agcgtgaag gtgggcgat ctgattatc gggactatt 
      gtaagcaaa acgcagtct gggatgatg cttaaggtc 
      ttgtgcacc gcagggcct gggcccaat atcatctat 
      gccgccgat atcaatgta aaggccttc tgcccggtg 
      gcaaatata ataccggcg gttgataag aagggcata 
      actcgcagc tacatgatg aatgattca gtttgctgc 
      gaagtgatt gaaaagatt ccagactcg gataagatg 
      gtttgttgc atgaagggc acacccaga aaaccaaac 
      gacccagaa cgtcgtcca ccgttggga ttaatcagt 
      gctgacgat tttcccttg gcttataag aaagctttg 
      gatcacaag aatgaaaac tatgacaca gtcctagaa 
      aagagcatt ggtttcaac aatccgaat aacatttcg 
      tatttggcg ggagttgtg ggaatcaaa gatcattgc 
      acgctcatg aagggacta aaggggcga tttgccgaa 
      aatgaatac gcgaatgaa ctgcgtcaa gttcaagcg 
      agcaaatgg gcgtatcgt aacgtagcc gatggtatt 
      gaccatttc aaagctggc aaacatgtc gaagctttc 
      caatgtcta aacaaagca ttaaacatt gatcctaag 
      aacgtagaa ggtctggta gcacgtggc gctttatat 
      gccaacagc ggcaatttt caaaaagcc atcgccgac 
      tttgaaacc gctctcaaa ctaaaccca aaccacggc 
      aatgctcgt aagtatatg ggcgaaacg cttgtagca 
      ctcggtcga agttacgaa gaagaagat aaaatcgaa 
      gaagcgaga aaatcatac cagagttgc ctaagtttg 
      atacctaat cacgaagaa gcccaaaat tctttaaag 
      tttttgaag aataaaact tccgcaaca aagaatcta 
      atagaaccg accgaattg ttactacca aatttgtcg 
      actgcgtcg aaaacagcc gatgtaaat gatgcactg 
      aaacaattg ttgaaaaat gaagacgag gagaagaaa 
      gataagaaa aagaaaaag aagagcaaa aaacgaaag 
      aaccgtcga cattcgagt tctagttcc agttcaagt 
      tcgtcggga agcaacgac agttcgagc agctcatcg 
      tcgagttct agcgactcg agttcaagt tccggcgat 
      agcgatttc aaaaaacgc aagaaacat cgttctcga 
      tcacaccgt cgcgagaaa caaagttcc ttaagtcca 
      ttaagtaaa cgaatggct atgatggat caatcgcac 
      gatacccct aatacttac acgtttaat aagccggcg 
      gctaccagt gccatgttc gattttaat ctcgaacaa 
      ccgagcgaa actcaaaaa cctgcagat ttaagttac 
      gaacagaga ttgagaatg ttcttacaa gagactaaa 
      ggtgattcc gattatgag gaaaaggta aggaaattt 
      ttagaggag agttcaaag tggaagaag gagaaacgc 
      agcgatgag aagaagaag aaacgtaag aaggacaga 
      aagaacaag aaggatagt aaaaagaag aagaaggag 
      aagaaattg aaacggaaa ggcttcgat ttgagcgag 
      ttcgaggat cacaaaaag ttgagagac gcaattaaa 
      aaggagttg agtaagggg aaaaagtcg aagaagcac 
      agtatacca tcggacgat gatgagtac ttcttgcaa 
      aaggcggga tataccaag cggtttatc gatagctta 
      cctgatttg gaagagcta gagtcaaaa ttgaatgcc 
      tactatgca atggaaaaa gaatcgaag agaagtgaa 
      ctgagcgag agtcccaag cgcggtctt cttgactca 
      ccttcgccc acgcgcgag cagaaagcc cgtcaggcg 
      gtggccgaa gccgccgcc accgccggt tctgctaac 
      aaatggaaa atgcaagtc gcttcccaa ccgcctcca 
      tctacgagc ggttctcgt ttcaaaaag aaaccggat 
      cgtgaagaa tggaaagag gaacagcaa caacaacag 
      atgatggat aaacgtggc ggtggtggt ggtggtggt 
      atggcgtca ggtggaaaa aataataat ttcgaagaa 
      tcagttcga aagaatcaa caagctccc gtgcctccg 
      cctgagaaa aatatgagc gtacgcaag gcgatggca 
      atgaaggaa ccgccgcca ccaaaacga cccgattcg 
      ccgaaaaac aaaccgaag ttattgccg cctataccg 
      gcggttaat ccgctcgtg aacccggga caggtggtt 
      ctggacaag ttcggtaat ttcaggttg atgacgccg 
      ccggagttg aagaaggga cgagatgat atgccgccg 
      ttaccacca ggtcgccgt ccgcccgag ccgccaggt 
      cgcccccgt tccgacagc cgttcgccg aagcgtcat 
      agcagatcc cgctctcgc cgcactcgt tcgtcgagc 
      gcctctcgt tctagatcc cgcagttac cggagccgt 
      tcccggtcg tattatagc cgttccagg tcacgttcc 
      ggttcttac tattctaga agtcgttcc cgctcacgt 
      agctactct ggagaccgt cgtcggtac agacgtggc 
      ggtttcaga ggccggtac aacgaccga ggaacttac 
      tacaagcct cgcttccag aatcctcgc aacaacatc 
      cgaggcaga ggaggcggt ggcggcggt ggcggttac 
      tataaccgc gatggcagg tataacgat agggagtac 
      agagggcgt ggtaggccg tataataac tataacaac 
      agaggggga agaagaccg agaggtaga taccaaaga 
      ggcggctac agagagtat agagatagg agggattat 
      agaagtaga tcgagggat aggagtagg tctatgagc 
      ggaagccga gatagggac tcggacgag gcgatgaaa 
      aaggcggcg aatgcagaa aaggataat aaggttaac 
      aagtacgcg gacaacgaa ggagagcaa ataaggcat 
      gaaggaccg ttgtcagaa ggagaagac agagatgac 
      tatacatcg aacgagaag tttgtggat agagaggcg 
      ttcggcggg aagtgggca gaaaaggaa gaaggtgga 
      ggacaggat aatggtggt ggaggagat ggagatggc 
      gcgaaagtg gacaagact aacattccg tcggaaagt 
      aacatcgaa gagatggat aaatttctg actaaagtt 
      aagaaagat aagaaggaa gagatgttg gaaagaaat 
      aaagacttg ctgaagaat aagtcttaa
    mRNA sequence
    • >TC014056 RA
      ggcaacgga aatagacaa tccgccatt ttgggagtt 
      tttcgttgg tgtacgaag cggtgtggt ctttgatta 
      tcaaatatt ttgatttat tctagggct tgaagcaac 
      gctccacca tggagtcgt tgaacacga accttctga 
      gccagtcgc tgaatttca atggacagc aaatgcaaa 
      agttgtggg aggctgaat acggcgaga atgatttgc 
      accgtagga acgtcaagg atgttaact tccaagtgt 
      acagcgaga gacagaagc atttgtctt tccaggatc 
      gaggaaaac gactcaaac tccagcagt ttgtcgtca 
      agaaagcaa acatgttgt ttgcgactg aagcttccg 
      attttgagt ataaacctg acgaggggc ccgttagtg 
      aggacacgt acgcgatca tgcccccat ttgagacgt 
      ttcttaatg ttgacaagc agcaaaggc tcaaatatt 
      tctttaaga gcgtgaagg tgggcgatc tgattatcg 
      ggactattg taagcaaaa cgcagtctg ggatgatgc 
      ttaaggtct tgtgcaccg cagggcctg ggcccaata 
      tcatctatg ccgccgata tcaatgtaa aggccttct 
      gcccggtgg caaatataa taccggcgg ttgataaga 
      agggcataa ctcgcagct acatgatga atgattcag 
      tttgctgcg aagtgattg aaaagattc cagactcgg 
      ataagatgg tttgttgca tgaagggca cacccagaa 
      aaccaaacg acccagaac gtcgtccac cgttgggat 
      taatcagtg ctgacgatt ttcccttgg cttataaga 
      aagctttgg atcacaaga atgaaaact atgacacag 
      tcctagaaa agagcattg gtttcaaca atccgaata 
      acatttcgt atttggcgg gagttgtgg gaatcaaag 
      atcattgca cgctcatga agggactaa aggggcgat 
      ttgccgaaa atgaatacg cgaatgaac tgcgtcaag 
      ttcaagcga gcaaatggg cgtatcgta acgtagccg 
      atggtattg accatttca aagctggca aacatgtcg 
      aagctttcc aatgtctaa acaaagcat taaacattg 
      atcctaaga acgtagaag gtctggtag cacgtggcg 
      ctttatatg ccaacagcg gcaattttc aaaaagcca 
      tcgccgact ttgaaaccg ctctcaaac taaacccaa 
      accacggca atgctcgta agtatatgg gcgaaacgc 
      ttgtagcac tcggtcgaa gttacgaag aagaagata 
      aaatcgaag aagcgagaa aatcatacc agagttgcc 
      taagtttga tacctaatc acgaagaag cccaaaatt 
      ctttaaagt ttttgaaga ataaaactt ccgcaacaa 
      agaatctaa tagaaccga ccgaattgt tactaccaa 
      atttgtcga ctgcgtcga aaacagccg atgtaaatg 
      atgcactga aacaattgt tgaaaaatg aagacgagg 
      agaagaaag ataagaaaa agaaaaaga agagcaaaa 
      aacgaaaga accgtcgac attcgagtt ctagttcca 
      gttcaagtt cgtcgggaa gcaacgaca gttcgagca 
      gctcatcgt cgagttcta gcgactcga gttcaagtt 
      ccggcgata gcgatttca aaaaacgca agaaacatc 
      gttctcgat cacaccgtc gcgagaaac aaagttcct 
      taagtccat taagtaaac gaatggcta tgatggatc 
      aatcgcacg ataccccta atacttaca cgtttaata 
      agccggcgg ctaccagtg ccatgttcg attttaatc 
      tcgaacaac cgagcgaaa ctcaaaaac ctgcagatt 
      taagttacg aacagagat tgagaatgt tcttacaag 
      agactaaag gtgattccg attatgagg aaaaggtaa 
      ggaaatttt tagaggaga gttcaaagt ggaagaagg 
      agaaacgca gcgatgaga agaagaaga aacgtaaga 
      aggacagaa agaacaaga aggatagta aaaagaaga 
      agaaggaga agaaattga aacggaaag gcttcgatt 
      tgagcgagt tcgaggatc acaaaaagt tgagagacg 
      caattaaaa aggagttga gtaagggga aaaagtcga 
      agaagcaca gtataccat cggacgatg atgagtact 
      tcttgcaaa aggcgggat ataccaagc ggtttatcg 
      atagcttac ctgatttgg aagagctag agtcaaaat 
      tgaatgcct actatgcaa tggaaaaag aatcgaaga 
      gaagtgaac tgagcgaga gtcccaagc gcggtcttc 
      ttgactcac cttcgccca cgcgcgagc agaaagccc 
      gtcaggcgg tggccgaag ccgccgcca ccgccggtt 
      ctgctaaca aatggaaaa tgcaagtcg cttcccaac 
      cgcctccat ctacgagcg gttctcgtt tcaaaaaga 
      aaccggatc gtgaagaat ggaaagagg aacagcaac 
      aacaacaga tgatggata aacgtggcg gtggtggtg 
      gtggtggta tggcgtcag gtggaaaaa ataataatt 
      tcgaagaat cagttcgaa agaatcaac aagctcccg 
      tgcctccgc ctgagaaaa atatgagcg tacgcaagg 
      cgatggcaa tgaaggaac cgccgccac caaaacgac 
      ccgattcgc cgaaaaaca aaccgaagt tattgccgc 
      ctataccgg cggttaatc cgctcgtga acccgggac 
      aggtggttc tggacaagt tcggtaatt tcaggttga 
      tgacgccgc cggagttga agaagggac gagatgata 
      tgccgccgt taccaccag gtcgccgtc cgcccgagc 
      cgccaggtc gcccccgtt ccgacagcc gttcgccga 
      agcgtcata gcagatccc gctctcgcc gcactcgtt 
      cgtcgagcg cctctcgtt ctagatccc gcagttacc 
      ggagccgtt cccggtcgt attatagcc gttccaggt 
      cacgttccg gttcttact attctagaa gtcgttccc 
      gctcacgta gctactctg gagaccgtc gtcggtaca 
      gacgtggcg gtttcagag gccggtaca acgaccgag 
      gaacttact acaagcctc gcttccaga atcctcgca 
      acaacatcc gaggcagag gaggcggtg gcggcggtg 
      gcggttact ataaccgcg atggcaggt ataacgata 
      gggagtaca gagggcgtg gtaggccgt ataataact 
      ataacaaca gagggggaa gaagaccga gaggtagat 
      accaaagag gcggctaca gagagtata gagatagga 
      gggattata gaagtagat cgagggata ggagtaggt 
      ctatgagcg gaagccgag atagggact cggacgagg 
      cgatgaaaa aggcggcga atgcagaaa aggataata 
      aggttaaca agtacgcgg acaacgaag gagagcaaa 
      taaggcatg aaggaccgt tgtcagaag gagaagaca 
      gagatgact atacatcga acgagaagt ttgtggata 
      gagaggcgt tcggcggga agtgggcag aaaaggaag 
      aaggtggag gacaggata atggtggtg gaggagatg 
      gagatggcg cgaaagtgg acaagacta acattccgt 
      cggaaagta acatcgaag agatggata aatttctga 
      ctaaagtta agaaagata agaaggaag agatgttgg 
      aaagaaata aagacttgc tgaagaata agtcttaat 
      tagttttgt gttgtatag atactttaa atgtttaat 
      tattagatt gagcttttt ttctttgat ttgttttag 
      tcttcatga tgctaaatg tgtttctac tgtgtactg 
      atttattct taatccaca ataaatgta atcagttca 
      a
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