| General information | |||
| TC014056 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 5010448..5016431 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014056 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05548 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC014056 RA
MESLNTNLL SQSLNFNGQ QMQKLWEAE YGENDLHRR NVKDVNFQV YSERQKHLS FQDRGKRLK LQQFVVKKA NMLFATEAS DFEYKPDEG PVSEDTYAI MPPFETFLN VDKQQRLKY FFKSVKVGD LIIGTIVSK TQSGMMLKV LCTAGPGPN IIYAADINV KAFCPVANI IPAVDKKGI TRSYMMNDS VCCEVIEKI PDSDKMVCC MKGTPRKPN DPERRPPLG LISADDFPL AYKKALDHK NENYDTVLE KSIGFNNPN NISYLAGVV GIKDHCTLM KGLKGRFAE NEYANELRQ VQASKWAYR NVADGIDHF KAGKHVEAF QCLNKALNI DPKNVEGLV ARGALYANS GNFQKAIAD FETALKLNP NHGNARKYM GETLVALGR SYEEEDKIE EARKSYQSC LSLIPNHEE AQNSLKFLK NKTSATKNL IEPTELLLP NLSTASKTA DVNDALKQL LKNEDEEKK DKKKKKKSK KRKNRRHSS SSSSSSSSG SNDSSSSSS SSSSDSSSS SGDSDFKKR KKHRSRSHR REKQSSLSP LSKRMAMMD QSHDTPNTY TFNKPAATS AMFDFNLEQ PSETQKPAD LSYEQRLRM FLQETKGDS DYEEKVRKF LEESSKWKK EKRSDEKKK KRKKDRKNK KDSKKKKKE KKLKRKGFD LSEFEDHKK LRDAIKKEL SKGKKSKKH SIPSDDDEY FLQKAGYTK RFIDSLPDL EELESKLNA YYAMEKESK RSELSESPK RGLLDSPSP TREQKARQA VAEAAATAG SANKWKMQV ASQPPPSTS GSRFKKKPD REEWKEEQQ QQQMMDKRG GGGGGGMAS GGKNNNFEE SVRKNQQAP VPPPEKNMS VRKAMAMKE PPPPKRPDS PKNKPKLLP PIPAVNPLV NPGQVVLDK FGNFRLMTP PELKKGRDD MPPLPPGRR PPEPPGRPR SDSRSPKRH SRSRSRRTR SSSASRSRS RSYRSRSRS YYSRSRSRS GSYYSRSRS RSRSYSGDR RRYRRGGFR GRYNDRGTY YKPRFQNPR NNIRGRGGG GGGGGYYNR DGRYNDREY RGRGRPYNN YNNRGGRRP RGRYQRGGY REYRDRRDY RSRSRDRSR SMSGSRDRD SDEAMKKAA NAEKDNKVN KYADNEGEQ IRHEGPLSE GEDRDDYTS NEKFVDREA FGGKWAEKE EGGGQDNGG GGDGDGAKV DKTNIPSES NIEEMDKFL TKVKKDKKE EMLERNKDL LKNKS
- >TC014056 RA
atggagtcg ttgaacacg aaccttctg agccagtcg ctgaatttc aatggacag caaatgcaa aagttgtgg gaggctgaa tacggcgag aatgatttg caccgtagg aacgtcaag gatgttaac ttccaagtg tacagcgag agacagaag catttgtct ttccaggat cgaggaaaa cgactcaaa ctccagcag tttgtcgtc aagaaagca aacatgttg tttgcgact gaagcttcc gattttgag tataaacct gacgagggg cccgttagt gaggacacg tacgcgatc atgccccca tttgagacg tttcttaat gttgacaag cagcaaagg ctcaaatat ttctttaag agcgtgaag gtgggcgat ctgattatc gggactatt gtaagcaaa acgcagtct gggatgatg cttaaggtc ttgtgcacc gcagggcct gggcccaat atcatctat gccgccgat atcaatgta aaggccttc tgcccggtg gcaaatata ataccggcg gttgataag aagggcata actcgcagc tacatgatg aatgattca gtttgctgc gaagtgatt gaaaagatt ccagactcg gataagatg gtttgttgc atgaagggc acacccaga aaaccaaac gacccagaa cgtcgtcca ccgttggga ttaatcagt gctgacgat tttcccttg gcttataag aaagctttg gatcacaag aatgaaaac tatgacaca gtcctagaa aagagcatt ggtttcaac aatccgaat aacatttcg tatttggcg ggagttgtg ggaatcaaa gatcattgc acgctcatg aagggacta aaggggcga tttgccgaa aatgaatac gcgaatgaa ctgcgtcaa gttcaagcg agcaaatgg gcgtatcgt aacgtagcc gatggtatt gaccatttc aaagctggc aaacatgtc gaagctttc caatgtcta aacaaagca ttaaacatt gatcctaag aacgtagaa ggtctggta gcacgtggc gctttatat gccaacagc ggcaatttt caaaaagcc atcgccgac tttgaaacc gctctcaaa ctaaaccca aaccacggc aatgctcgt aagtatatg ggcgaaacg cttgtagca ctcggtcga agttacgaa gaagaagat aaaatcgaa gaagcgaga aaatcatac cagagttgc ctaagtttg atacctaat cacgaagaa gcccaaaat tctttaaag tttttgaag aataaaact tccgcaaca aagaatcta atagaaccg accgaattg ttactacca aatttgtcg actgcgtcg aaaacagcc gatgtaaat gatgcactg aaacaattg ttgaaaaat gaagacgag gagaagaaa gataagaaa aagaaaaag aagagcaaa aaacgaaag aaccgtcga cattcgagt tctagttcc agttcaagt tcgtcggga agcaacgac agttcgagc agctcatcg tcgagttct agcgactcg agttcaagt tccggcgat agcgatttc aaaaaacgc aagaaacat cgttctcga tcacaccgt cgcgagaaa caaagttcc ttaagtcca ttaagtaaa cgaatggct atgatggat caatcgcac gatacccct aatacttac acgtttaat aagccggcg gctaccagt gccatgttc gattttaat ctcgaacaa ccgagcgaa actcaaaaa cctgcagat ttaagttac gaacagaga ttgagaatg ttcttacaa gagactaaa ggtgattcc gattatgag gaaaaggta aggaaattt ttagaggag agttcaaag tggaagaag gagaaacgc agcgatgag aagaagaag aaacgtaag aaggacaga aagaacaag aaggatagt aaaaagaag aagaaggag aagaaattg aaacggaaa ggcttcgat ttgagcgag ttcgaggat cacaaaaag ttgagagac gcaattaaa aaggagttg agtaagggg aaaaagtcg aagaagcac agtatacca tcggacgat gatgagtac ttcttgcaa aaggcggga tataccaag cggtttatc gatagctta cctgatttg gaagagcta gagtcaaaa ttgaatgcc tactatgca atggaaaaa gaatcgaag agaagtgaa ctgagcgag agtcccaag cgcggtctt cttgactca ccttcgccc acgcgcgag cagaaagcc cgtcaggcg gtggccgaa gccgccgcc accgccggt tctgctaac aaatggaaa atgcaagtc gcttcccaa ccgcctcca tctacgagc ggttctcgt ttcaaaaag aaaccggat cgtgaagaa tggaaagag gaacagcaa caacaacag atgatggat aaacgtggc ggtggtggt ggtggtggt atggcgtca ggtggaaaa aataataat ttcgaagaa tcagttcga aagaatcaa caagctccc gtgcctccg cctgagaaa aatatgagc gtacgcaag gcgatggca atgaaggaa ccgccgcca ccaaaacga cccgattcg ccgaaaaac aaaccgaag ttattgccg cctataccg gcggttaat ccgctcgtg aacccggga caggtggtt ctggacaag ttcggtaat ttcaggttg atgacgccg ccggagttg aagaaggga cgagatgat atgccgccg ttaccacca ggtcgccgt ccgcccgag ccgccaggt cgcccccgt tccgacagc cgttcgccg aagcgtcat agcagatcc cgctctcgc cgcactcgt tcgtcgagc gcctctcgt tctagatcc cgcagttac cggagccgt tcccggtcg tattatagc cgttccagg tcacgttcc ggttcttac tattctaga agtcgttcc cgctcacgt agctactct ggagaccgt cgtcggtac agacgtggc ggtttcaga ggccggtac aacgaccga ggaacttac tacaagcct cgcttccag aatcctcgc aacaacatc cgaggcaga ggaggcggt ggcggcggt ggcggttac tataaccgc gatggcagg tataacgat agggagtac agagggcgt ggtaggccg tataataac tataacaac agaggggga agaagaccg agaggtaga taccaaaga ggcggctac agagagtat agagatagg agggattat agaagtaga tcgagggat aggagtagg tctatgagc ggaagccga gatagggac tcggacgag gcgatgaaa aaggcggcg aatgcagaa aaggataat aaggttaac aagtacgcg gacaacgaa ggagagcaa ataaggcat gaaggaccg ttgtcagaa ggagaagac agagatgac tatacatcg aacgagaag tttgtggat agagaggcg ttcggcggg aagtgggca gaaaaggaa gaaggtgga ggacaggat aatggtggt ggaggagat ggagatggc gcgaaagtg gacaagact aacattccg tcggaaagt aacatcgaa gagatggat aaatttctg actaaagtt aagaaagat aagaaggaa gagatgttg gaaagaaat aaagacttg ctgaagaat aagtcttaa
- >TC014056 RA
ggcaacgga aatagacaa tccgccatt ttgggagtt tttcgttgg tgtacgaag cggtgtggt ctttgatta tcaaatatt ttgatttat tctagggct tgaagcaac gctccacca tggagtcgt tgaacacga accttctga gccagtcgc tgaatttca atggacagc aaatgcaaa agttgtggg aggctgaat acggcgaga atgatttgc accgtagga acgtcaagg atgttaact tccaagtgt acagcgaga gacagaagc atttgtctt tccaggatc gaggaaaac gactcaaac tccagcagt ttgtcgtca agaaagcaa acatgttgt ttgcgactg aagcttccg attttgagt ataaacctg acgaggggc ccgttagtg aggacacgt acgcgatca tgcccccat ttgagacgt ttcttaatg ttgacaagc agcaaaggc tcaaatatt tctttaaga gcgtgaagg tgggcgatc tgattatcg ggactattg taagcaaaa cgcagtctg ggatgatgc ttaaggtct tgtgcaccg cagggcctg ggcccaata tcatctatg ccgccgata tcaatgtaa aggccttct gcccggtgg caaatataa taccggcgg ttgataaga agggcataa ctcgcagct acatgatga atgattcag tttgctgcg aagtgattg aaaagattc cagactcgg ataagatgg tttgttgca tgaagggca cacccagaa aaccaaacg acccagaac gtcgtccac cgttgggat taatcagtg ctgacgatt ttcccttgg cttataaga aagctttgg atcacaaga atgaaaact atgacacag tcctagaaa agagcattg gtttcaaca atccgaata acatttcgt atttggcgg gagttgtgg gaatcaaag atcattgca cgctcatga agggactaa aggggcgat ttgccgaaa atgaatacg cgaatgaac tgcgtcaag ttcaagcga gcaaatggg cgtatcgta acgtagccg atggtattg accatttca aagctggca aacatgtcg aagctttcc aatgtctaa acaaagcat taaacattg atcctaaga acgtagaag gtctggtag cacgtggcg ctttatatg ccaacagcg gcaattttc aaaaagcca tcgccgact ttgaaaccg ctctcaaac taaacccaa accacggca atgctcgta agtatatgg gcgaaacgc ttgtagcac tcggtcgaa gttacgaag aagaagata aaatcgaag aagcgagaa aatcatacc agagttgcc taagtttga tacctaatc acgaagaag cccaaaatt ctttaaagt ttttgaaga ataaaactt ccgcaacaa agaatctaa tagaaccga ccgaattgt tactaccaa atttgtcga ctgcgtcga aaacagccg atgtaaatg atgcactga aacaattgt tgaaaaatg aagacgagg agaagaaag ataagaaaa agaaaaaga agagcaaaa aacgaaaga accgtcgac attcgagtt ctagttcca gttcaagtt cgtcgggaa gcaacgaca gttcgagca gctcatcgt cgagttcta gcgactcga gttcaagtt ccggcgata gcgatttca aaaaacgca agaaacatc gttctcgat cacaccgtc gcgagaaac aaagttcct taagtccat taagtaaac gaatggcta tgatggatc aatcgcacg ataccccta atacttaca cgtttaata agccggcgg ctaccagtg ccatgttcg attttaatc tcgaacaac cgagcgaaa ctcaaaaac ctgcagatt taagttacg aacagagat tgagaatgt tcttacaag agactaaag gtgattccg attatgagg aaaaggtaa ggaaatttt tagaggaga gttcaaagt ggaagaagg agaaacgca gcgatgaga agaagaaga aacgtaaga aggacagaa agaacaaga aggatagta aaaagaaga agaaggaga agaaattga aacggaaag gcttcgatt tgagcgagt tcgaggatc acaaaaagt tgagagacg caattaaaa aggagttga gtaagggga aaaagtcga agaagcaca gtataccat cggacgatg atgagtact tcttgcaaa aggcgggat ataccaagc ggtttatcg atagcttac ctgatttgg aagagctag agtcaaaat tgaatgcct actatgcaa tggaaaaag aatcgaaga gaagtgaac tgagcgaga gtcccaagc gcggtcttc ttgactcac cttcgccca cgcgcgagc agaaagccc gtcaggcgg tggccgaag ccgccgcca ccgccggtt ctgctaaca aatggaaaa tgcaagtcg cttcccaac cgcctccat ctacgagcg gttctcgtt tcaaaaaga aaccggatc gtgaagaat ggaaagagg aacagcaac aacaacaga tgatggata aacgtggcg gtggtggtg gtggtggta tggcgtcag gtggaaaaa ataataatt tcgaagaat cagttcgaa agaatcaac aagctcccg tgcctccgc ctgagaaaa atatgagcg tacgcaagg cgatggcaa tgaaggaac cgccgccac caaaacgac ccgattcgc cgaaaaaca aaccgaagt tattgccgc ctataccgg cggttaatc cgctcgtga acccgggac aggtggttc tggacaagt tcggtaatt tcaggttga tgacgccgc cggagttga agaagggac gagatgata tgccgccgt taccaccag gtcgccgtc cgcccgagc cgccaggtc gcccccgtt ccgacagcc gttcgccga agcgtcata gcagatccc gctctcgcc gcactcgtt cgtcgagcg cctctcgtt ctagatccc gcagttacc ggagccgtt cccggtcgt attatagcc gttccaggt cacgttccg gttcttact attctagaa gtcgttccc gctcacgta gctactctg gagaccgtc gtcggtaca gacgtggcg gtttcagag gccggtaca acgaccgag gaacttact acaagcctc gcttccaga atcctcgca acaacatcc gaggcagag gaggcggtg gcggcggtg gcggttact ataaccgcg atggcaggt ataacgata gggagtaca gagggcgtg gtaggccgt ataataact ataacaaca gagggggaa gaagaccga gaggtagat accaaagag gcggctaca gagagtata gagatagga gggattata gaagtagat cgagggata ggagtaggt ctatgagcg gaagccgag atagggact cggacgagg cgatgaaaa aggcggcga atgcagaaa aggataata aggttaaca agtacgcgg acaacgaag gagagcaaa taaggcatg aaggaccgt tgtcagaag gagaagaca gagatgact atacatcga acgagaagt ttgtggata gagaggcgt tcggcggga agtgggcag aaaaggaag aaggtggag gacaggata atggtggtg gaggagatg gagatggcg cgaaagtgg acaagacta acattccgt cggaaagta acatcgaag agatggata aatttctga ctaaagtta agaaagata agaaggaag agatgttgg aaagaaata aagacttgc tgaagaata agtcttaat tagttttgt gttgtatag atactttaa atgtttaat tattagatt gagcttttt ttctttgat ttgttttag tcttcatga tgctaaatg tgtttctac tgtgtactg atttattct taatccaca ataaatgta atcagttca a
