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Gene information
General information
TC014046 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4868827..4872376
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014046
    iBeetle screen iB_05547

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 7
    egg chamber potentially number decreased - Number of animals showing the phenotype: 4

    egg chamber number increased - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    wings not closed - Number of animals showing the phenotype: 10

    wings distal bent - Number of animals showing the phenotype: 3
    wings slightly orientation irregular - Number of animals showing the phenotype: 7
    Fertility
    egg production: number no/almost no eggs -
    ovary size decreased - Number of animals showing the phenotype: 4

    tropharium size decreased - Number of animals showing the phenotype: 4
    egg chamber not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    Comment: remaining cells seem to show no polyploidy (small nuclei)
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014046 RA
      MLIKDAPVI WEGLRSIIC VYKPPSVTC ERVRKTIIA 
      AVCKGLNEM ECRPPVDFV CIEGNPSGK LTVSVKPSL 
      ADDPLVVGP RYIHDDLKC SWSNFLGFN TSGVLLLGL 
      GKGTKVAKL IRENRPTRC YRLSGKLGE ATDNAFKDG 
      KVVERTTWR HVKLENMNR FLATLQASH QKEMFKSCG 
      IDLQSEEAY KLACQGPIR PANSKLPVI YGIKCVKFE 
      PPEFVIEIQ CINEYETYL RSLIHEIGM KLHSSAHCT 
      AIQCIRHSY FTVENALLR KHWTLQDII TNMEQCIQI 
      MDEHENIIR QKSAALQ
    CDS sequence
    • >TC014046 RA
      atgttaata aaagacgcc cccgtgatt tgggagggt 
      ctccggtcc attatttgt gtttacaag cctcctagt 
      gtaacatgt gaacgcgta cgcaaaact atcattgcc 
      gctgtttgt aaaggttta aatgagatg gagtgtcgc 
      cctccagtt gatttcgtt tgtattgaa ggaaatcca 
      tcgggaaaa ttaaccgta tctgtaaaa ccaagtctc 
      gccgatgac cctcttgtt gtgggacct cgttacatc 
      cacgatgat ttaaaatgt agttggtcg aatttttta 
      ggttttaat acgagcgga gtgcttttg ttaggatta 
      gggaaaggc accaaagta gcgaaattg ataagagag 
      aacagaccc actaggtgt taccgattg tccggaaaa 
      ttgggcgaa gcgaccgat aacgccttt aaggacggg 
      aaagttgtg gaaaggaca acctggaga cacgttaaa 
      ctagaaaat atgaatagg tttttagca acacttcaa 
      gcttcacac caaaaagaa atgtttaag tcatgtggc 
      attgatttg caatcagag gaggcctat aaattggct 
      tgtcaggga cccatcagg cctgccaac tcaaaactt 
      ccagtcatt tacggaatt aaatgtgtt aaatttgaa 
      cccccagag ttcgtcatt gaaatacaa tgcataaat 
      gaatatgaa acttacctg agaagttta atacatgaa 
      attgggatg aaattacat tcttcagcg cattgtacg 
      gcaattcag tgcatcaga catagttat tttactgta 
      gaaaatgca cttttgcgc aaacactgg actttgcag 
      gacattatt acaaacatg gaacaatgt atacagatt 
      atggatgaa cacgaaaat attatcaga caaaagtcg 
      gcagcttta cagtag
    mRNA sequence
    • >TC014046 RA
      agggttaaa gtgtacgct agttgttac gcaatggaa 
      gggactgaa aatttagac gatgaagtc tacaacaaa 
      agacacagt cggttggaa aacgacgaa cgtaggaga 
      aagcgatgg gacgttcaa aggataaga gaacagcgg 
      gtaatagaa aaattgaag cagagacag gaaagaatg 
      ggttcgggg tctcggacg gatgaacaa atcgagtcc 
      gtgctgtct ctttggccc actttagac aacattaaa 
      tatctggaa gtctctgat cagctgcca gtggcggcc 
      tttggctca cctattccc aaaatagcc cccagtgaa 
      tttagtttg ccatggttg gctaaccct gcgctaatc 
      acgaataag agagggtca acaaaacga acaacagtg 
      agaaagaaa aactcaaag agataagta ttatgtcaa 
      caaccgaaa taaaatttt agtcaagca ccactgcct 
      gatgttttt gtaaataaa aataaatta tgaagtggt 
      ggaatgaac gaaaatgtg attttatga atacataca 
      ggttatttg taaattgaa acaatctca ttacatgac 
      agtttgtaa agtatttat ttaggttgt aagcttact 
      tttggagta gattactag gcgccgtaa taccaattt 
      aggactcca tattagggt tttaggttg cgaatttta 
      atttttaag taatgtaat attgtgata tcggaattg 
      ttatgtcca tagatttcc tgataatga tattaattt 
      acaaataat gaatcatat atttttagt tgaagcgcg 
      ttaaataaa taaatgtat tagttgtac ttatttgtg 
      tatcagtca ctgtattta aatatacac aattttttc 
      gaatcattt tgcttatgc cctcaagaa aaaaaaatg 
      gtcctgacc agggattga acctcagac gcggtcgga 
      aaaattaca catcacggc cttacactt ctttgacgc 
      gaggtggca gcaccatag ttattttaa atactaacc 
      taactttta cgtaaacat aaaaatgtt aataaaaga 
      cgcccccgt gatttggga gggtctccg gtccattat 
      ttgtgttta caagcctcc tagtgtaac atgtgaacg 
      cgtacgcaa aactatcat tgccgctgt ttgtaaagg 
      tttaaatga gatggagtg tcgccctcc agttgattt 
      cgtttgtat tgaaggaaa tccatcggg aaaattaac 
      cgtatctgt aaaaccaag tctcgccga tgaccctct 
      tgttgtggg acctcgtta catccacga tgatttaaa 
      atgtagttg gtcgaattt tttaggttt taatacgag 
      cggagtgct tttgttagg attagggaa aggcaccaa 
      agtagcgaa attgataag agagaacag acccactag 
      gtgttaccg attgtccgg aaaattggg cgaagcgac 
      cgataacgc ctttaagga cgggaaagt tgtggaaag 
      gacaacctg gagacacgt taaactaga aaatatgaa 
      taggttttt agcaacact tcaagcttc acaccaaaa 
      agaaatgtt taagtcatg tggcattga tttgcaatc 
      agaggaggc ctataaatt ggcttgtca gggacccat 
      caggcctgc caactcaaa acttccagt catttacgg 
      aattaaatg tgttaaatt tgaaccccc agagttcgt 
      cattgaaat acaatgcat aaatgaata tgaaactta 
      cctgagaag tttaataca tgaaattgg gatgaaatt 
      acattcttc agcgcattg tacggcaat tcagtgcat 
      cagacatag ttattttac tgtagaaaa tgcactttt 
      gcgcaaaca ctggacttt gcaggacat tattacaaa 
      catggaaca atgtataca gattatgga tgaacacga 
      aaatattat cagacaaaa gtcggcagc tttacagta 
      gtttattca attgtcttc tgtaaaaat catttattt 
      tacgttaaa tttgtttgt tttgtcaaa aaataaaat 
      atccaaagc agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY