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Gene information
General information
TC014032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4525345..4527420
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014032
    iBeetle screen iB_05545

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    about 25

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014032 RA
      MSLFYVTFL FLAINSAIA SPVLQFKPT PRQSDSGLI 
      WFVPQTRTT YRHYVEDLD KFLTRYKNG QKPANVWKC 
      DPNETPPQN VICDVDVRA LGPCSTAPD YCFYRAQIC 
      IFLKLSHVT NWVPEFYNS TNWPSDMPL SLKQTIHEE 
      EERGTHRKI WVTCDGETP ADKEMIGAL DYKPSQGFS 
      RQYFSITDT KNYLSPLIA VHFERPRTG VLINVECAA 
      WARNIPRKN KQGIARFGL LLEDMLKIK TVKTSSRMQ 
      LIYLLVLIS GARCCQSRW NSNKTPVLV LRPSTETNL 
      IWFKPTDQK RQHYKTDLN NLLKRYNNT KQFENVVSC 
      LPHQSPPHN KICNFDVNS NVMAPCLPK FGFGYDFGT 
      PCVFLKLSN VPKWRPVPY NSSNMPSEM PNFLKDTIT 
      KLEPRGMHQ NIWVSCDGD TAADKEHIG ALRYLPFNG 
      FPSQYFPYN GDKEYLDPL VALHFEQPM RGVLINVEC 
      AVWARNIPR DKENKLGIL QFSLMID
    CDS sequence
    • >TC014032 RA
      atgtcacta ttttacgtg acgttcctc ttcctggcg 
      ataaacagt gccatcgca agcccagtt ttgcaattc 
      aaacctaca ccaagacaa agcgactca ggtttgatt 
      tggtttgtg ccacaaact cgaaccaca tatcggcat 
      tatgtcgaa gatttagac aaattcctt acaaggtac 
      aagaacggg caaaagcct gcaaatgtg tggaaatgt 
      gacccaaat gagacacct ccccaaaat gtaatttgt 
      gatgtagat gttagagct ttgggacct tgtagtaca 
      gcacctgat tactgtttt tatcgcgca caaatctgc 
      atttttctc aaattaagc cacgtcacg aattgggtc 
      ccggagttt tataacagc acaaactgg ccctccgat 
      atgccactc tctttgaaa cagactata cacgaagag 
      gaagaaaga ggaacccac cggaaaatt tgggtgact 
      tgtgacggg gaaactccg gctgataaa gagatgatt 
      ggagccttg gattacaag ccatcgcag ggcttttcc 
      cgccaatat ttttccatt acagatacc aaaaattat 
      ctcagtcct ttgatcgct gttcatttt gaacgacca 
      cgaaccggg gttctgatt aatgtcgaa tgcgcagcg 
      tgggcgagg aacatacca cggaaaaac aagcaagga 
      attgctcga tttggatta ttgcttgaa gacatgtta 
      aaaattaaa actgttaaa acctcgtcc agaatgcaa 
      ttaatttat ttgctggtt ttaatttct ggcgctaga 
      tgctgccaa agccgctgg aactcaaac aaaacgcca 
      gttttggta ttgagaccc tcgacagaa acgaacctc 
      atttggttc aagccaacc gaccaaaaa cgccaacat 
      tacaaaacc gatttaaat aacttactc aaacgttac 
      aataacact aaacaattt gagaatgtg gtttcctgt 
      cttccacac caatcacct ccacacaac aaaatttgt 
      aattttgac gtcaattca aatgtaatg gccccctgt 
      ctaccaaaa tttggtttt gggtacgat tttggcaca 
      ccttgtgtc ttcctaaaa ctgagtaat gttccaaaa 
      tggagacct gtgccttac aacagctcg aacatgccg 
      tcagaaatg ccaaatttt ttgaaagac accatcaca 
      aaactggaa cctcgagga atgcaccaa aatatttgg 
      gtgagttgt gatggcgac actgcagcc gataaggaa 
      cacatcggg gctttaaga tatctgcct tttaatggc 
      tttccaagt caatatttt ccgtataat ggcgataag 
      gagtatctt gacccttta gtggctctt catttcgag 
      caacccatg cgtggtgtt ttgattaat gtggaatgt 
      gccgtatgg gcgagaaat ataccacgt gacaaggaa 
      aacaaactg ggaattttg cagttttcg ttgatgata 
      gactaa
    mRNA sequence
    • >TC014032 RA
      cttttcatc aaaactcaa taaaatgtc actatttta 
      cgtgacgtt cctcttcct ggcgataaa cagtgccat 
      cgcaagccc agttttgca attcaaacc tacaccaag 
      acaaagcga ctcaggttt gatttggtt tgtgccaca 
      aactcgaac cacatatcg gcattatgt cgaagattt 
      agacaaatt ccttacaag gtacaagaa cgggcaaaa 
      gcctgcaaa tgtgtggaa atgtgaccc aaatgagac 
      acctcccca aaatgtaat ttgtgatgt agatgttag 
      agctttggg accttgtag tacagcacc tgattactg 
      tttttatcg cgcacaaat ctgcatttt tctcaaatt 
      aagccacgt cacgaattg ggtcccgga gttttataa 
      cagcacaaa ctggccctc cgatatgcc actctcttt 
      gaaacagac tatacacga agaggaaga aagaggaac 
      ccaccggaa aatttgggt gacttgtga cggggaaac 
      tccggctga taaagagat gattggagc cttggatta 
      caagccatc gcagggctt ttcccgcca atatttttc 
      cattacaga taccaaaaa ttatctcag tcctttgat 
      cgctgttca ttttgaacg accacgaac cggggttct 
      gattaatgt cgaatgcgc agcgtgggc gaggaacat 
      accacggaa aaacaagca aggaattgc tcgatttgg 
      attattgct tgaagacat gttaaaaat taaaactgt 
      taaaacctc gtccagaat gcaattaat ttatttgct 
      ggttttaat ttctggcgc tagatgctg ccaaagccg 
      ctggaactc aaacaaaac gccagtttt ggtattgag 
      accctcgac agaaacgaa cctcatttg gttcaagcc 
      aaccgacca aaaacgcca acattacaa aaccgattt 
      aaataactt actcaaacg ttacaataa cactaaaca 
      atttgagaa tgtggtttc ctgtcttcc acaccaatc 
      acctccaca caacaaaat ttgtaattt tgacgtcaa 
      ttcaaatgt aatggcccc ctgtctacc aaaatttgg 
      ttttgggta cgattttgg cacaccttg tgtcttcct 
      aaaactgag taatgttcc aaaatggag acctgtgcc 
      ttacaacag ctcgaacat gccgtcaga aatgccaaa 
      ttttttgaa agacaccat cacaaaact ggaacctcg 
      aggaatgca ccaaaatat ttgggtgag ttgtgatgg 
      cgacactgc agccgataa ggaacacat cggggcttt 
      aagatatct gccttttaa tggctttcc aagtcaata 
      ttttccgta taatggcga taaggagta tcttgaccc 
      tttagtggc tcttcattt cgagcaacc catgcgtgg 
      tgttttgat taatgtgga atgtgccgt atgggcgag 
      aaatatacc acgtgacaa ggaaaacaa actgggaat 
      tttgcagtt ttcgttgat gatagacta ataataaat 
      tagtctaat tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY