Status
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Gene information
General information
TC014024 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4389859..4421486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014024
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: many L1-L2 animals. First larval molt is potentially disrupted.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    gula surface irregular - Number of animals showing the phenotype: 5


    wings not closed - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014024 RA
      MPSVLEEVA VEQSNAYTP FVDASGITV VPPSPSSKP 
      LKSIGDVQS LLQQGRKQD VKHLLRNNA WPVDSPIRG 
      QLWSLLCIQ HQNKSNSMS DGFYWDMVN QVFGSTELP 
      DKPISLPPF VEPSHCQLY YLTHRGRSV ADRVVSVLG 
      YACPDIVYS PTIYPICAL LLHYMSEEQ CYHCMASLV 
      ASKEKTFIT QTKLLYEVT WKTVMQICK KHVKGAAVH 
      LQRHCNSSR AERIYMDWI WWIFQGLPF QHLVRVMDC 
      FFHEGIKVL YRTAMAILI LFHKSSSSP SSEWLMEIQ 
      KNGIDSALN KFCKEMPVT PQKVLKVAF GIRGLSSAY 
      ISRVFIRTE MVLKSKNVI NGSRQLVRS RSSENLPNS 
      QSQNNIQMV SHTLTIREL LTLWSWLPV RITMYQPIL 
      LYTTEEHGC SLTTFYVRV EHHEPTLLM IKTCNNEVF 
      GAYCSSRWG ERNQKDDRG NRQAYFGTG ETFLFSLYP 
      ERAKYQWVG IEGDKVGHG AELFMAADS KMITIGGGE 
      GQAIWMDEN IRFGKTDRC QTFNNPPLC PSGDFEIRV 
      LEVYGFATD QMS
    CDS sequence
    • >TC014024 RA
      atgccttcg gtgttggaa gaagtggcc gtcgaacaa 
      tcgaacgct tacacaccg tttgtggat gccagcggt 
      ataacggtg gtgccacca agcccttct tccaaacct 
      ctgaagagc atcggcgac gtgcagtct cttcttcag 
      caaggacga aagcaagat gtcaagcat ttgctcaga 
      aacaacgcg tggcccgtt gacagcccc atacgggga 
      cagttgtgg tcccttctg tgcatccaa catcagaat 
      aaatcaaat agcatgtcg gacggtttc tactgggat 
      atggtcaat caagtcttt ggcagcaca gaattgcca 
      gataagccc atcagcttg ccaccgttt gtcgaacca 
      tcgcattgc caactttac tatctgact catagggga 
      aggagtgtc gctgatagg gtggtctca gtgctgggc 
      tatgcctgc ccagatatc gtctacagc cccactatc 
      taccccata tgcgcgctt ttgctccac tacatgtca 
      gaagaacaa tgctaccat tgcatggcc agtctggtg 
      gcctccaag gaaaaaacc ttcatcaca cagacgaaa 
      ctcctgtat gaagtcaca tggaagacg gtgatgcag 
      atttgcaag aagcatgtg aaaggggcc gctgtgcac 
      ctccaaaga cactgcaat agttcgcga gccgaacgc 
      atctacatg gactggatc tggtggata tttcaggga 
      ctccccttc cagcatttg gtcagagtg atggactgt 
      ttttttcac gagggtatt aaagtgctg tataggacg 
      gcgatggcg attttgatc ctttttcac aaatcgtca 
      tcatcgcct tcctccgaa tggctaatg gagatccag 
      aagaacggt atcgactca gccttgaac aaattctgc 
      aaagaaatg cccgtgacg ccgcagaag gtgttaaag 
      gttgcgttc ggtatccga ggcttgagt tcggcttac 
      atttcgcgg gtcttcatc cggaccgag atggttctc 
      aaaagcaaa aatgtgata aacgggtca cgacaattg 
      gtacggtcg cgatcaagc gaaaatctt ccaaacagc 
      cagagccag aacaacatc cagatggtg tcgcatacg 
      ctcaccatc agagagctc ttaacacta tggtcctgg 
      ttaccagtg aggataaca atgtaccag ccgatattg 
      ctatacacg acagaagag cacggatgt tcgttgaca 
      acgttctac gtccgtgtc gagcaccac gaaccgacg 
      ctgctcatg atcaaaacg tgcaacaac gaggttttc 
      ggtgcctat tgttcgtcc agatggggc gaaaggaac 
      caaaaggac gaccgaggc aaccgtcaa gcgtatttc 
      ggtacgggc gagacgttt ttgttctcg ctgtaccca 
      gaaagggcc aagtaccaa tgggttggt attgaaggt 
      gataaggtg ggacatggg gccgaattg ttcatggct 
      gccgattcg aaaatgatc acgatagga ggaggggag 
      ggtcaggcg atttggatg gacgaaaac atccgcttt 
      gggaagacc gaccggtgc caaactttc aacaaccct 
      cctctctgt ccgagtgga gatttcgaa atacgcgta 
      ctggaagtg tacggtttt gcgaccgat cagatgagt 
      tga
    mRNA sequence
    • >TC014024 RA
      cgttccttc aattgagtc cattaatct gtttacgat 
      ctgaacgtg tgtagtcgt gatattcgc gtccgtcat 
      taataagat gtgatcata acagtttta atagatgaa 
      attgttatt caaatcggc gcgcctacc tcgccacca 
      gtgcgagac agtgacagt tgtgtgaat gggagtggc 
      ttaatcgtc caggagacg aatgacagt tgttcacca 
      cagcaaacg tgatgttgc tcatctagt ccaaacagt 
      gatctggtt ttgagagag tggcgcgag tgaattcag 
      cccgattta ttcagtttt tgtggtgtt ttgtgactt 
      ggtgtttag tttggtgtt gtgccggaa gtggtgttc 
      gtgccccgg atcaatgtc ggcgaagag gtcttcggc 
      tccaatggc ccactgaga tcgacgcgt gaaaaatgt 
      tgcaggacc tcacatagc ggtctgccc gatcggagg 
      ggctgcaac tgtttaaaa aacaaaacg aatgcaaat 
      aatgccttc ggtgttgga agaagtggc cgtcgaaca 
      atcgaacgc ttacacacc gtttgtgga tgccagcgg 
      tataacggt ggtgccacc aagcccttc ttccaaacc 
      tctgaagag catcggcga cgtgcagtc tcttcttca 
      gcaaggacg aaagcaaga tgtcaagca tttgctcag 
      aaacaacgc gtggcccgt tgacagccc catacgggg 
      acagttgtg gtcccttct gtgcatcca acatcagaa 
      taaatcaaa tagcatgtc ggacggttt ctactggga 
      tatggtcaa tcaagtctt tggcagcac agaattgcc 
      agataagcc catcagctt gccaccgtt tgtcgaacc 
      atcgcattg ccaacttta ctatctgac tcatagggg 
      aaggagtgt cgctgatag ggtggtctc agtgctggg 
      ctatgcctg cccagatat cgtctacag ccccactat 
      ctaccccat atgcgcgct tttgctcca ctacatgtc 
      agaagaaca atgctacca ttgcatggc cagtctggt 
      ggcctccaa ggaaaaaac cttcatcac acagacgaa 
      actcctgta tgaagtcac atggaagac ggtgatgca 
      gatttgcaa gaagcatgt gaaaggggc cgctgtgca 
      cctccaaag acactgcaa tagttcgcg agccgaacg 
      catctacat ggactggat ctggtggat atttcaggg 
      actcccctt ccagcattt ggtcagagt gatggactg 
      tttttttca cgagggtat taaagtgct gtataggac 
      ggcgatggc gattttgat cctttttca caaatcgtc 
      atcatcgcc ttcctccga atggctaat ggagatcca 
      gaagaacgg tatcgactc agccttgaa caaattctg 
      caaagaaat gcccgtgac gccgcagaa ggtgttaaa 
      ggttgcgtt cggtatccg aggcttgag ttcggctta 
      catttcgcg ggtcttcat ccggaccga gatggttct 
      caaaagcaa aaatgtgat aaacgggtc acgacaatt 
      ggtacggtc gcgatcaag cgaaaatct tccaaacag 
      ccagagcca gaacaacat ccagatggt gtcgcatac 
      gctcaccat cagagagct cttaacact atggtcctg 
      gttaccagt gaggataac aatgtacca gccgatatt 
      gctatacac gacagaaga gcacggatg ttcgttgac 
      aacgttcta cgtccgtgt cgagcacca cgaaccgac 
      gctgctcat gatcaaaac gtgcaacaa cgaggtttt 
      cggtgccta ttgttcgtc cagatgggg cgaaaggaa 
      ccaaaagga cgaccgagg caaccgtca agcgtattt 
      cggtacggg cgagacgtt tttgttctc gctgtaccc 
      agaaagggc caagtacca atgggttgg tattgaagg 
      tgataaggt gggacatgg ggccgaatt gttcatggc 
      tgccgattc gaaaatgat cacgatagg aggagggga 
      gggtcaggc gatttggat ggacgaaaa catccgctt 
      tgggaagac cgaccggtg ccaaacttt caacaaccc 
      tcctctctg tccgagtgg agatttcga aatacgcgt 
      actggaagt gtacggttt tgcgaccga tcagatgag 
      ttgaaacga cgataatcg atcatcgaa caaagcgca 
      gtattttaa tgtttttgt ttggaagaa aacgaacat 
      ctcaatttt tgttttgtt acatgtttt tgttattgt 
      atattttta agacgatgt acctggttg gagaactat 
      cacgtgcat tccatcgag aagaagaaa aacaatgga 
      aggataaga aaggcggcg ccggcccga taacgtatg 
      aatgacaaa attctagtt tctagtcgt ttgttaagt 
      gttagaagt tttagttga gaagagtag attttacag 
      tatatactt ggtacaata gacacttta tttgccgta 
      ttttaagca taactgtat atatgttct ttatgtata 
      taatgtaaa ggtagcaaa gtaccaaag gtaacaatc 
      tctatcatt cttttttaa ttattatta ttagagaga 
      tgtttttaa tatttatta atacgtaat gtgccgatt 
      acgagatat ttgttcgtc taataatga cagttgacc 
      aagtcgact ttgatatta ttcattttt gacatttcc 
      atcgatcgc atggtgctc ttgataaag tcaaaaatt 
      gttaattcg ttgtgtttc tgttaagta ataatttat 
      taactcgat gtgctcaca tcccgaatc atatcatta 
      gagtcgaga ttgttggtg gttggatta ggtttagag 
      agcgttgaa attttgtac cgctgcatt ggtcacatc 
      acgtcgctt tcccgcata attatttgg acgcaactt 
      tagtcaatt tctaagcaa cgatgtatt ttaatttac 
      tgaatgttt atctcatgt gttgtattt taggaaaaa 
      agttccttt cgtgtcgta gaccgtgtc gcaatgtat 
      aagaaaata aaaacaaac aaatgcatg tccgttatt 
      ttcggtgtt ctcactgtg taactgatg cttttcgtg 
      tgttgtttt gtttcaatc aaagttttg ttggtaata 
      gtgccgcat aagcaataa ctgatgtga tactatttt 
      taatcaagg tttttaggt attacggtc ctgtgcgcg 
      tgttgacac aaccaattc gagccgttc ggccaattc 
      atttctcac gtaccataa tacgtaaac atcttggtc 
      gaccattgt gttaacaat ttatgtata tattaatat 
      tatttctcc tgactgttt tactgtagt taccagtct 
      tattctctg tactgaggt atctattat tgtattatg 
      cgtaatgag ttgttagtc ccaaaatat actgatatt 
      acttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY