General information | |||
TC014024 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 4389859..4421486 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014024 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_05544 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Comment: many L1-L2 animals. First larval molt is potentially disrupted. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
- >TC014024 RA
MPSVLEEVA VEQSNAYTP FVDASGITV VPPSPSSKP LKSIGDVQS LLQQGRKQD VKHLLRNNA WPVDSPIRG QLWSLLCIQ HQNKSNSMS DGFYWDMVN QVFGSTELP DKPISLPPF VEPSHCQLY YLTHRGRSV ADRVVSVLG YACPDIVYS PTIYPICAL LLHYMSEEQ CYHCMASLV ASKEKTFIT QTKLLYEVT WKTVMQICK KHVKGAAVH LQRHCNSSR AERIYMDWI WWIFQGLPF QHLVRVMDC FFHEGIKVL YRTAMAILI LFHKSSSSP SSEWLMEIQ KNGIDSALN KFCKEMPVT PQKVLKVAF GIRGLSSAY ISRVFIRTE MVLKSKNVI NGSRQLVRS RSSENLPNS QSQNNIQMV SHTLTIREL LTLWSWLPV RITMYQPIL LYTTEEHGC SLTTFYVRV EHHEPTLLM IKTCNNEVF GAYCSSRWG ERNQKDDRG NRQAYFGTG ETFLFSLYP ERAKYQWVG IEGDKVGHG AELFMAADS KMITIGGGE GQAIWMDEN IRFGKTDRC QTFNNPPLC PSGDFEIRV LEVYGFATD QMS
- >TC014024 RA
atgccttcg gtgttggaa gaagtggcc gtcgaacaa tcgaacgct tacacaccg tttgtggat gccagcggt ataacggtg gtgccacca agcccttct tccaaacct ctgaagagc atcggcgac gtgcagtct cttcttcag caaggacga aagcaagat gtcaagcat ttgctcaga aacaacgcg tggcccgtt gacagcccc atacgggga cagttgtgg tcccttctg tgcatccaa catcagaat aaatcaaat agcatgtcg gacggtttc tactgggat atggtcaat caagtcttt ggcagcaca gaattgcca gataagccc atcagcttg ccaccgttt gtcgaacca tcgcattgc caactttac tatctgact catagggga aggagtgtc gctgatagg gtggtctca gtgctgggc tatgcctgc ccagatatc gtctacagc cccactatc taccccata tgcgcgctt ttgctccac tacatgtca gaagaacaa tgctaccat tgcatggcc agtctggtg gcctccaag gaaaaaacc ttcatcaca cagacgaaa ctcctgtat gaagtcaca tggaagacg gtgatgcag atttgcaag aagcatgtg aaaggggcc gctgtgcac ctccaaaga cactgcaat agttcgcga gccgaacgc atctacatg gactggatc tggtggata tttcaggga ctccccttc cagcatttg gtcagagtg atggactgt ttttttcac gagggtatt aaagtgctg tataggacg gcgatggcg attttgatc ctttttcac aaatcgtca tcatcgcct tcctccgaa tggctaatg gagatccag aagaacggt atcgactca gccttgaac aaattctgc aaagaaatg cccgtgacg ccgcagaag gtgttaaag gttgcgttc ggtatccga ggcttgagt tcggcttac atttcgcgg gtcttcatc cggaccgag atggttctc aaaagcaaa aatgtgata aacgggtca cgacaattg gtacggtcg cgatcaagc gaaaatctt ccaaacagc cagagccag aacaacatc cagatggtg tcgcatacg ctcaccatc agagagctc ttaacacta tggtcctgg ttaccagtg aggataaca atgtaccag ccgatattg ctatacacg acagaagag cacggatgt tcgttgaca acgttctac gtccgtgtc gagcaccac gaaccgacg ctgctcatg atcaaaacg tgcaacaac gaggttttc ggtgcctat tgttcgtcc agatggggc gaaaggaac caaaaggac gaccgaggc aaccgtcaa gcgtatttc ggtacgggc gagacgttt ttgttctcg ctgtaccca gaaagggcc aagtaccaa tgggttggt attgaaggt gataaggtg ggacatggg gccgaattg ttcatggct gccgattcg aaaatgatc acgatagga ggaggggag ggtcaggcg atttggatg gacgaaaac atccgcttt gggaagacc gaccggtgc caaactttc aacaaccct cctctctgt ccgagtgga gatttcgaa atacgcgta ctggaagtg tacggtttt gcgaccgat cagatgagt tga
- >TC014024 RA
cgttccttc aattgagtc cattaatct gtttacgat ctgaacgtg tgtagtcgt gatattcgc gtccgtcat taataagat gtgatcata acagtttta atagatgaa attgttatt caaatcggc gcgcctacc tcgccacca gtgcgagac agtgacagt tgtgtgaat gggagtggc ttaatcgtc caggagacg aatgacagt tgttcacca cagcaaacg tgatgttgc tcatctagt ccaaacagt gatctggtt ttgagagag tggcgcgag tgaattcag cccgattta ttcagtttt tgtggtgtt ttgtgactt ggtgtttag tttggtgtt gtgccggaa gtggtgttc gtgccccgg atcaatgtc ggcgaagag gtcttcggc tccaatggc ccactgaga tcgacgcgt gaaaaatgt tgcaggacc tcacatagc ggtctgccc gatcggagg ggctgcaac tgtttaaaa aacaaaacg aatgcaaat aatgccttc ggtgttgga agaagtggc cgtcgaaca atcgaacgc ttacacacc gtttgtgga tgccagcgg tataacggt ggtgccacc aagcccttc ttccaaacc tctgaagag catcggcga cgtgcagtc tcttcttca gcaaggacg aaagcaaga tgtcaagca tttgctcag aaacaacgc gtggcccgt tgacagccc catacgggg acagttgtg gtcccttct gtgcatcca acatcagaa taaatcaaa tagcatgtc ggacggttt ctactggga tatggtcaa tcaagtctt tggcagcac agaattgcc agataagcc catcagctt gccaccgtt tgtcgaacc atcgcattg ccaacttta ctatctgac tcatagggg aaggagtgt cgctgatag ggtggtctc agtgctggg ctatgcctg cccagatat cgtctacag ccccactat ctaccccat atgcgcgct tttgctcca ctacatgtc agaagaaca atgctacca ttgcatggc cagtctggt ggcctccaa ggaaaaaac cttcatcac acagacgaa actcctgta tgaagtcac atggaagac ggtgatgca gatttgcaa gaagcatgt gaaaggggc cgctgtgca cctccaaag acactgcaa tagttcgcg agccgaacg catctacat ggactggat ctggtggat atttcaggg actcccctt ccagcattt ggtcagagt gatggactg tttttttca cgagggtat taaagtgct gtataggac ggcgatggc gattttgat cctttttca caaatcgtc atcatcgcc ttcctccga atggctaat ggagatcca gaagaacgg tatcgactc agccttgaa caaattctg caaagaaat gcccgtgac gccgcagaa ggtgttaaa ggttgcgtt cggtatccg aggcttgag ttcggctta catttcgcg ggtcttcat ccggaccga gatggttct caaaagcaa aaatgtgat aaacgggtc acgacaatt ggtacggtc gcgatcaag cgaaaatct tccaaacag ccagagcca gaacaacat ccagatggt gtcgcatac gctcaccat cagagagct cttaacact atggtcctg gttaccagt gaggataac aatgtacca gccgatatt gctatacac gacagaaga gcacggatg ttcgttgac aacgttcta cgtccgtgt cgagcacca cgaaccgac gctgctcat gatcaaaac gtgcaacaa cgaggtttt cggtgccta ttgttcgtc cagatgggg cgaaaggaa ccaaaagga cgaccgagg caaccgtca agcgtattt cggtacggg cgagacgtt tttgttctc gctgtaccc agaaagggc caagtacca atgggttgg tattgaagg tgataaggt gggacatgg ggccgaatt gttcatggc tgccgattc gaaaatgat cacgatagg aggagggga gggtcaggc gatttggat ggacgaaaa catccgctt tgggaagac cgaccggtg ccaaacttt caacaaccc tcctctctg tccgagtgg agatttcga aatacgcgt actggaagt gtacggttt tgcgaccga tcagatgag ttgaaacga cgataatcg atcatcgaa caaagcgca gtattttaa tgtttttgt ttggaagaa aacgaacat ctcaatttt tgttttgtt acatgtttt tgttattgt atattttta agacgatgt acctggttg gagaactat cacgtgcat tccatcgag aagaagaaa aacaatgga aggataaga aaggcggcg ccggcccga taacgtatg aatgacaaa attctagtt tctagtcgt ttgttaagt gttagaagt tttagttga gaagagtag attttacag tatatactt ggtacaata gacacttta tttgccgta ttttaagca taactgtat atatgttct ttatgtata taatgtaaa ggtagcaaa gtaccaaag gtaacaatc tctatcatt cttttttaa ttattatta ttagagaga tgtttttaa tatttatta atacgtaat gtgccgatt acgagatat ttgttcgtc taataatga cagttgacc aagtcgact ttgatatta ttcattttt gacatttcc atcgatcgc atggtgctc ttgataaag tcaaaaatt gttaattcg ttgtgtttc tgttaagta ataatttat taactcgat gtgctcaca tcccgaatc atatcatta gagtcgaga ttgttggtg gttggatta ggtttagag agcgttgaa attttgtac cgctgcatt ggtcacatc acgtcgctt tcccgcata attatttgg acgcaactt tagtcaatt tctaagcaa cgatgtatt ttaatttac tgaatgttt atctcatgt gttgtattt taggaaaaa agttccttt cgtgtcgta gaccgtgtc gcaatgtat aagaaaata aaaacaaac aaatgcatg tccgttatt ttcggtgtt ctcactgtg taactgatg cttttcgtg tgttgtttt gtttcaatc aaagttttg ttggtaata gtgccgcat aagcaataa ctgatgtga tactatttt taatcaagg tttttaggt attacggtc ctgtgcgcg tgttgacac aaccaattc gagccgttc ggccaattc atttctcac gtaccataa tacgtaaac atcttggtc gaccattgt gttaacaat ttatgtata tattaatat tatttctcc tgactgttt tactgtagt taccagtct tattctctg tactgaggt atctattat tgtattatg cgtaatgag ttgttagtc ccaaaatat actgatatt acttca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
