Status
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Gene information
General information
TC014024 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4389859..4421486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014024
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: many L1-L2 animals. First larval molt is potentially disrupted.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    Adult morphology
    gula surface irregular - Number of animals showing the phenotype: 5


    wings not closed - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014024 RA
      MPSVLEEVA VEQSNAYTP FVDASGITV VPPSPSSKP 
LKSIGDVQS LLQQGRKQD VKHLLRNNA WPVDSPIRG 
QLWSLLCIQ HQNKSNSMS DGFYWDMVN QVFGSTELP 
DKPISLPPF VEPSHCQLY YLTHRGRSV ADRVVSVLG 
YACPDIVYS PTIYPICAL LLHYMSEEQ CYHCMASLV 
ASKEKTFIT QTKLLYEVT WKTVMQICK KHVKGAAVH 
LQRHCNSSR AERIYMDWI WWIFQGLPF QHLVRVMDC 
FFHEGIKVL YRTAMAILI LFHKSSSSP SSEWLMEIQ 
KNGIDSALN KFCKEMPVT PQKVLKVAF GIRGLSSAY 
ISRVFIRTE MVLKSKNVI NGSRQLVRS RSSENLPNS 
QSQNNIQMV SHTLTIREL LTLWSWLPV RITMYQPIL 
LYTTEEHGC SLTTFYVRV EHHEPTLLM IKTCNNEVF 
GAYCSSRWG ERNQKDDRG NRQAYFGTG ETFLFSLYP 
ERAKYQWVG IEGDKVGHG AELFMAADS KMITIGGGE 
GQAIWMDEN IRFGKTDRC QTFNNPPLC PSGDFEIRV 
LEVYGFATD QMS
    CDS sequence
    • >TC014024 RA
      atgccttcg gtgttggaa gaagtggcc gtcgaacaa 
tcgaacgct tacacaccg tttgtggat gccagcggt 
ataacggtg gtgccacca agcccttct tccaaacct 
ctgaagagc atcggcgac gtgcagtct cttcttcag 
caaggacga aagcaagat gtcaagcat ttgctcaga 
aacaacgcg tggcccgtt gacagcccc atacgggga 
cagttgtgg tcccttctg tgcatccaa catcagaat 
aaatcaaat agcatgtcg gacggtttc tactgggat 
atggtcaat caagtcttt ggcagcaca gaattgcca 
gataagccc atcagcttg ccaccgttt gtcgaacca 
tcgcattgc caactttac tatctgact catagggga 
aggagtgtc gctgatagg gtggtctca gtgctgggc 
tatgcctgc ccagatatc gtctacagc cccactatc 
taccccata tgcgcgctt ttgctccac tacatgtca 
gaagaacaa tgctaccat tgcatggcc agtctggtg 
gcctccaag gaaaaaacc ttcatcaca cagacgaaa 
ctcctgtat gaagtcaca tggaagacg gtgatgcag 
atttgcaag aagcatgtg aaaggggcc gctgtgcac 
ctccaaaga cactgcaat agttcgcga gccgaacgc 
atctacatg gactggatc tggtggata tttcaggga 
ctccccttc cagcatttg gtcagagtg atggactgt 
ttttttcac gagggtatt aaagtgctg tataggacg 
gcgatggcg attttgatc ctttttcac aaatcgtca 
tcatcgcct tcctccgaa tggctaatg gagatccag 
aagaacggt atcgactca gccttgaac aaattctgc 
aaagaaatg cccgtgacg ccgcagaag gtgttaaag 
gttgcgttc ggtatccga ggcttgagt tcggcttac 
atttcgcgg gtcttcatc cggaccgag atggttctc 
aaaagcaaa aatgtgata aacgggtca cgacaattg 
gtacggtcg cgatcaagc gaaaatctt ccaaacagc 
cagagccag aacaacatc cagatggtg tcgcatacg 
ctcaccatc agagagctc ttaacacta tggtcctgg 
ttaccagtg aggataaca atgtaccag ccgatattg 
ctatacacg acagaagag cacggatgt tcgttgaca 
acgttctac gtccgtgtc gagcaccac gaaccgacg 
ctgctcatg atcaaaacg tgcaacaac gaggttttc 
ggtgcctat tgttcgtcc agatggggc gaaaggaac 
caaaaggac gaccgaggc aaccgtcaa gcgtatttc 
ggtacgggc gagacgttt ttgttctcg ctgtaccca 
gaaagggcc aagtaccaa tgggttggt attgaaggt 
gataaggtg ggacatggg gccgaattg ttcatggct 
gccgattcg aaaatgatc acgatagga ggaggggag 
ggtcaggcg atttggatg gacgaaaac atccgcttt 
gggaagacc gaccggtgc caaactttc aacaaccct 
cctctctgt ccgagtgga gatttcgaa atacgcgta 
ctggaagtg tacggtttt gcgaccgat cagatgagt 
tga
    mRNA sequence
    • >TC014024 RA
      cgttccttc aattgagtc cattaatct gtttacgat 
ctgaacgtg tgtagtcgt gatattcgc gtccgtcat 
taataagat gtgatcata acagtttta atagatgaa 
attgttatt caaatcggc gcgcctacc tcgccacca 
gtgcgagac agtgacagt tgtgtgaat gggagtggc 
ttaatcgtc caggagacg aatgacagt tgttcacca 
cagcaaacg tgatgttgc tcatctagt ccaaacagt 
gatctggtt ttgagagag tggcgcgag tgaattcag 
cccgattta ttcagtttt tgtggtgtt ttgtgactt 
ggtgtttag tttggtgtt gtgccggaa gtggtgttc 
gtgccccgg atcaatgtc ggcgaagag gtcttcggc 
tccaatggc ccactgaga tcgacgcgt gaaaaatgt 
tgcaggacc tcacatagc ggtctgccc gatcggagg 
ggctgcaac tgtttaaaa aacaaaacg aatgcaaat 
aatgccttc ggtgttgga agaagtggc cgtcgaaca 
atcgaacgc ttacacacc gtttgtgga tgccagcgg 
tataacggt ggtgccacc aagcccttc ttccaaacc 
tctgaagag catcggcga cgtgcagtc tcttcttca 
gcaaggacg aaagcaaga tgtcaagca tttgctcag 
aaacaacgc gtggcccgt tgacagccc catacgggg 
acagttgtg gtcccttct gtgcatcca acatcagaa 
taaatcaaa tagcatgtc ggacggttt ctactggga 
tatggtcaa tcaagtctt tggcagcac agaattgcc 
agataagcc catcagctt gccaccgtt tgtcgaacc 
atcgcattg ccaacttta ctatctgac tcatagggg 
aaggagtgt cgctgatag ggtggtctc agtgctggg 
ctatgcctg cccagatat cgtctacag ccccactat 
ctaccccat atgcgcgct tttgctcca ctacatgtc 
agaagaaca atgctacca ttgcatggc cagtctggt 
ggcctccaa ggaaaaaac cttcatcac acagacgaa 
actcctgta tgaagtcac atggaagac ggtgatgca 
gatttgcaa gaagcatgt gaaaggggc cgctgtgca 
cctccaaag acactgcaa tagttcgcg agccgaacg 
catctacat ggactggat ctggtggat atttcaggg 
actcccctt ccagcattt ggtcagagt gatggactg 
tttttttca cgagggtat taaagtgct gtataggac 
ggcgatggc gattttgat cctttttca caaatcgtc 
atcatcgcc ttcctccga atggctaat ggagatcca 
gaagaacgg tatcgactc agccttgaa caaattctg 
caaagaaat gcccgtgac gccgcagaa ggtgttaaa 
ggttgcgtt cggtatccg aggcttgag ttcggctta 
catttcgcg ggtcttcat ccggaccga gatggttct 
caaaagcaa aaatgtgat aaacgggtc acgacaatt 
ggtacggtc gcgatcaag cgaaaatct tccaaacag 
ccagagcca gaacaacat ccagatggt gtcgcatac 
gctcaccat cagagagct cttaacact atggtcctg 
gttaccagt gaggataac aatgtacca gccgatatt 
gctatacac gacagaaga gcacggatg ttcgttgac 
aacgttcta cgtccgtgt cgagcacca cgaaccgac 
gctgctcat gatcaaaac gtgcaacaa cgaggtttt 
cggtgccta ttgttcgtc cagatgggg cgaaaggaa 
ccaaaagga cgaccgagg caaccgtca agcgtattt 
cggtacggg cgagacgtt tttgttctc gctgtaccc 
agaaagggc caagtacca atgggttgg tattgaagg 
tgataaggt gggacatgg ggccgaatt gttcatggc 
tgccgattc gaaaatgat cacgatagg aggagggga 
gggtcaggc gatttggat ggacgaaaa catccgctt 
tgggaagac cgaccggtg ccaaacttt caacaaccc 
tcctctctg tccgagtgg agatttcga aatacgcgt 
actggaagt gtacggttt tgcgaccga tcagatgag 
ttgaaacga cgataatcg atcatcgaa caaagcgca 
gtattttaa tgtttttgt ttggaagaa aacgaacat 
ctcaatttt tgttttgtt acatgtttt tgttattgt 
atattttta agacgatgt acctggttg gagaactat 
cacgtgcat tccatcgag aagaagaaa aacaatgga 
aggataaga aaggcggcg ccggcccga taacgtatg 
aatgacaaa attctagtt tctagtcgt ttgttaagt 
gttagaagt tttagttga gaagagtag attttacag 
tatatactt ggtacaata gacacttta tttgccgta 
ttttaagca taactgtat atatgttct ttatgtata 
taatgtaaa ggtagcaaa gtaccaaag gtaacaatc 
tctatcatt cttttttaa ttattatta ttagagaga 
tgtttttaa tatttatta atacgtaat gtgccgatt 
acgagatat ttgttcgtc taataatga cagttgacc 
aagtcgact ttgatatta ttcattttt gacatttcc 
atcgatcgc atggtgctc ttgataaag tcaaaaatt 
gttaattcg ttgtgtttc tgttaagta ataatttat 
taactcgat gtgctcaca tcccgaatc atatcatta 
gagtcgaga ttgttggtg gttggatta ggtttagag 
agcgttgaa attttgtac cgctgcatt ggtcacatc 
acgtcgctt tcccgcata attatttgg acgcaactt 
tagtcaatt tctaagcaa cgatgtatt ttaatttac 
tgaatgttt atctcatgt gttgtattt taggaaaaa 
agttccttt cgtgtcgta gaccgtgtc gcaatgtat 
aagaaaata aaaacaaac aaatgcatg tccgttatt 
ttcggtgtt ctcactgtg taactgatg cttttcgtg 
tgttgtttt gtttcaatc aaagttttg ttggtaata 
gtgccgcat aagcaataa ctgatgtga tactatttt 
taatcaagg tttttaggt attacggtc ctgtgcgcg 
tgttgacac aaccaattc gagccgttc ggccaattc 
atttctcac gtaccataa tacgtaaac atcttggtc 
gaccattgt gttaacaat ttatgtata tattaatat 
tatttctcc tgactgttt tactgtagt taccagtct 
tattctctg tactgaggt atctattat tgtattatg 
cgtaatgag ttgttagtc ccaaaatat actgatatt 
acttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY