Status
Please wait ...
Gene information
General information
TC033046
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014012,TC014011
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4099791..4150963
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033046
    iBeetle screen iB_05542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    iBeetle screen iB_05541

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033046 RA
      MHGNANDRR ALEREALRN VINQWNANR LDLFELSEP 
      NEDLEFHGV MRFYFQDSG QKVATKCIR VASDATVED 
      VIGTLVEKF RPDMRMLSV PSYALYELH EGCPERRML 
      PDEKPLLVQ LNWHVDDRE GRFLLKNID DKTTNSVGS 
      LDSNASFRR KLSKREKKQ LKKQEKLSR MKSENGDIN 
      DKEDGVAEK LYTELPETS FTRSISNPE AVMRRRRQQ 
      KLEKKLQQF RSKDGGPDT GGTLKIYGE ALCKDVPYK 
      TLLLSVRDC AGAVVREML EKYGLNKTD PSQWCLVQV 
      TQQPGVPDT EYVLDDDEC PLSILMNSP NTGSIMFHV 
      RRRPADCAP RKRKKKPGA LGTNTNNPR DVRDAVPLL 
      VELGPDGSA PHPGDTART IRLTNDVME VGSANGIAL 
      QLYGPHIQP RHCVITNAD GVTSLTPCH ADAHTYVNG 
      QRIHQTTIL NHGSLVKFG NSNTYRFLD PVHEDRMRH 
      PAVMDNARI YDSRSPRAL SPTEPSREN IETTFDLEG 
      NVETISTAS GAREDNRSL GSASENRLS GMDRYPRGQ 
      DPILPAVLE FPEDPQEQF LARVITHLD VTTPTFRLA 
      PAYTLYLCA RYRASTHYR PELTPTERA HKLTVLLQH 
      AAMLIRHTV QDRSTESIS QAFWLANAS ELLHFLKSD 
      RHVSAFSIQ AQDTLADAV QLAFKNLVA CLTDELNSA 
      MAHLMSVTG DDHPREVIN ILSGAMNLL RKCRVNAAL 
      TIQLFSQLF HWISAKALA NVISNSNLC TRSFGNKLF 
      NRLRNLQAW AESQGLELA AECHLAKIV QCAHLMQAP 
      KYTPEDLAN LSSACFKLN SMQLGALLR QYKAEPGEK 
      LAPPPLLEQ AAKAAESVA DELARAEGR EVTLYEDPA 
      PPLQLLLPD DGFSCDVVQ GVPPGLADF LHPLQASGL 
      CRLAAQPTS SGHWTVYMS APRPPSALS TTQPEVQVI 
      RLHKAGGGM GLSIVAAKG AGQERLGIY IKSVVPGGA 
      ADRDGRLAA GDQLLSVDG QSLLGITQE KAAEFLVRT 
      GSVVTLEVA KGGAVYHGL ATLLQQPSP TPHRAGDFY 
      AESYYSSQE SLYSLNNCL VFTPPATKP KNNVFFSSD 
      DTLYKKETR KFTIPRIEV ESDEPVFNL QVTDFSEPS 
      PKNIILGYD ETGTNFETK KVEDFTVIS NKSWKDKAN 
      NFVQQKSYS IDIPENPFV DIGQVGDTD KFLSISKKS 
      RQQKLCSSS LQDLSSSTS SINSGVHES NLDLSHIDP 
      DTPIKHKNW KSPDEVRFG QVDSQRFES KATSYPDLS 
      DYKSFGSKS KSVSEERLN DKLTEYERL ELLKLLHDW 
      SLNGSYSKS DFNIRLTKS SEHLEKENL ETSEKSLRR 
      SSVKFSSEP NLSPKFDIV IVKYSSEDN IKEKKATDF 
      DLMHKCEFR NCIFNEDFG KVAKPKGIL KNSRERLQE 
      TECLNNLAN QKTPKLGPN CRRYSEIIE PKSFNSQLV 
      RCDSLERLT HLNKKSGHK KFPDSYIVT RRNNQRNKN 
      SPKKSPKII VLKKKYLPK TWKSCSDIK HRKTVRKCC 
      RYAKKNCPI LRNSSETSR KTQSCVDIE RDYAARLAG 
      DSQVGAFDC PRRMSERDL PSRVDSERT IPSSKSVPA 
      LHHISPNSE QPRNPHDVF NPGYSRTSS NNSINTNSA 
      PPANGPVNN QAGLRSRST HNLHQQDGG FYQNLSVYR 
      NKIPSPQQL GDRTSALRS SQNSLQSSV VNSQRPASA 
      YYPPPMSVN ASRSNLHQS IPNLKSPPS THRIHNEES 
      RSGSYPSVN QQHHPQNYP GPNHPYYYD PNRPPQAQP 
      ITNYPNNVQ HVHSNGQRQ DYNNVGGLL REDMVRQSQ 
      NARHEEIMR YLSTNNARI NEQNRIEEM RVSKSDDLL 
      KHHQANDLM KYASSGNIR AHESSKVEI PLYRMKDEE 
      ELDKDMRMA NNVRSQKLV ANAKQQRVM QYQHPNNQY 
      YAPYHFQMA QNTQSMQNL NLNQYQYYP TSPTYKSPP 
      VAPKPSKRP DEPPELPPT STHPLYSAS SQDPPKMAF 
      YPTSTVPST KTPRDPWAR EEQERQAEA RREASRQWQ 
      EQQIRELMA LPQRTPQQE EQLRVLQLE REFQRRAME 
      AAEQDDDDT EKESPSPSM SQPVQNPPP KQEPPPSIL 
      KPGAVNTQT QPPPEPVAP PPPERGSSF AVMSMRAKE 
      NTKRVSFDT SPPQPPPNT LQLEETVRE DPNSFIRQA 
      ESMLASPTT PTDASPGAA VATPGVIGA QEVYRDPRA 
      RRLAEQAQQ KNQTMPPVP EKLSFKEKM KMFAMETGE 
      QETPKDKSK ISRAQREID NLGSPSSGL VH
    CDS sequence
    • >TC033046 RA
      atgcatggt aacgccaac gaccgcagg gccctcgaa 
      agagaggct ctgcgaaat gttatcaac caatggaac 
      gccaatcga ctggatctg ttcgagtta tccgaaccg 
      aatgaggac ttggaattc cacggtgtt atgcgattt 
      tacttccaa gactcgggg caaaaagtc gccaccaag 
      tgcatacga gtggcttcg gacgccact gtggaagac 
      gtgatcgga actttggtc gaaaaattc cgacccgac 
      atgaggatg ttatcagtg ccttcctac gctctctac 
      gaactccat gagggatgc cccgaacga cgaatgctt 
      cccgacgag aaaccgctc cttgtgcag ctcaactgg 
      cacgtggac gaccgcgag ggacggttt ctcctcaaa 
      aatattgac gacaaaacg accaactcg gtcggttct 
      ctcgactcg aatgcgagt tttcggcga aaattatcg 
      aagcgagaa aagaagcag ttgaagaaa caggaaaaa 
      ctctcgaga atgaaaagc gaaaatggt gatattaac 
      gataaggaa gacggcgtt gcggaaaaa ttatacaca 
      gaattgccc gaaacgtcg ttcacgcgc tcgatttcg 
      aacccggag gcggtgatg aggcggcgg aggcaacaa 
      aaactggaa aagaaattg cagcagttt aggtcgaag 
      gatggaggg cctgatacg ggtggcact ttgaaaatc 
      tatggcgag gccttatgt aaagacgtg ccttacaaa 
      accctcctc ctctcagtg cgggattgc gctggtgct 
      gtggtccgg gaaatgttg gaaaaatac ggtctcaac 
      aaaaccgac ccatcgcag tggtgtctc gtccaagtg 
      acccaacaa ccaggagtc cctgacact gaatacgtc 
      ctcgacgat gacgaatgc cccttatct attctaatg 
      aactcgcca aacaccggc tccatcatg tttcacgtt 
      aggagacga ccggcagat tgcgctccc cgaaaaaga 
      aagaaaaaa ccaggggcg ctaggcacc aacaccaat 
      aatccgaga gatgttcgg gatgctgtg ccccttttg 
      gtcgaattg gggccagat gggtccgcc ccgcacccg 
      ggggacacc gccaggacg atccgactc acaaacgat 
      gtcatggaa gtgggatca gcgaacggc atcgccctg 
      cagctctac ggcccccac atccagccc cggcattgc 
      gtcatcacc aacgccgac ggggtgaca tctttgaca 
      ccttgtcat gcggacgcc catacctac gttaatggc 
      cagcgcatc catcaaacc acaatttta aaccatgga 
      agtcttgtt aaatttggc aacagcaac acctatcga 
      tttctagat ccggttcac gaagacaga atgaggcat 
      ccagccgtt atggataat gccaggata tacgatagc 
      cggtcgccg cgagctctc agtcctact gagccctct 
      cgggaaaat atcgaaacg acgtttgat ctggaagga 
      aatgttgaa acgatttcg acggccagt ggagccaga 
      gaggataac aggtcgctc ggatcggca tccgaaaat 
      aggttgagc ggcatggac cgctacccc cgcggccag 
      gaccctatc ctccccgcc gtgctggag ttccccgag 
      gacccccag gagcagttc ctagctcgc gttataacg 
      catctcgac gtcacaaca ccaacattt cgcctcgcc 
      ccagcttac actctatac ctctgtgcc cgttatcga 
      gcatcgact cactaccgc cccgaacta acaccaaca 
      gaacgtgcc cacaaactc accgttttg cttcaacac 
      gccgccatg ctgatccga cacacggtc caagaccga 
      agcaccgaa agcatatct caagctttc tggttagcc 
      aacgccagt gaattacta cacttcctc aaatcggac 
      agacacgtc agtgcgttc tcgatccaa gcacaagac 
      acacttgca gatgcagta caattagct tttaagaat 
      ttagtcgcg tgtttaacc gacgagtta aactcggcc 
      atggctcat ttgatgtcg gtgacaggg gatgaccat 
      ccccgcgaa gttataaat attttaagt ggtgctatg 
      aatctccta cggaagtgt cgggtcaat gcggcccta 
      accatccaa ttattttcc cagttgttc cactggatt 
      tcggccaaa gcgctcgca aatgtgatc agtaacagt 
      aatttatgt acgagaagt ttcggcaat aaactcttc 
      aatcgatta aggaacctg caagcttgg gctgagtcc 
      caaggcttg gagttggct gcggagtgc caccttgcc 
      aagatcgtg caatgtgca cacttgatg caagcacct 
      aaatacacg ccggaggat ttagccaac ttgtcgtcg 
      gcctgtttc aaattaaat tcgatgcaa ttgggggct 
      ttattaaga cagtacaag gcggaacct ggggagaaa 
      ttggcaccg cccccgttg ctggagcag gcggccaaa 
      gcggccgaa agcgtcgcc gacgagctt gcgcgggct 
      gaaggtcgc gaagtcaca ctttacgaa gacccagcg 
      ccaccacta caactttta ctccccgat gatgggttt 
      agctgcgat gtcgttcaa ggggttcca ccaggtctt 
      gccgacttc ctgcacccg ctgcaagcg tcgggtttg 
      tgtcgtctg gccgcgcaa ccgacgagt tcgggccat 
      tggacagtg tacatgtcc gcgccacgg cctcccagt 
      gctttgagt acgacccaa ccagaggtc caagtcatt 
      cggttgcat aaggctggg ggtggcatg ggcttgtcg 
      atagtggca gccaaagga gccggtcag gagcgactc 
      ggaatttat ataaaatcg gtggttccc ggaggagct 
      gcagatcga gatgggcga ttggctgct ggcgatcaa 
      ctactatca gtcgatgga caatccctt ctgggaatc 
      acacaagaa aaagccgcc gagtttctg gtacggacc 
      ggaagtgtt gtcacactt gaagtagcg aaagggggt 
      gctgtgtat cacggattg gccacattg cttcaacag 
      ccgagtcct acacctcat cgagctggg gatttctac 
      gctgaatcc tactacagc tcgcaagag tccctctac 
      agcctaaac aattgcttg gtgttcacg cccccggcc 
      accaaaccc aaaaacaac gtatttttc agctccgat 
      gacactctt tacaaaaaa gaaacgcgc aaattcacc 
      atccctcga atcgaagtc gagagtgac gaacccgtt 
      ttcaatcta caagtaacc gatttttcc gaaccatcc 
      cccaaaaac attattctg gggtacgac gaaacgggt 
      accaacttt gaaacgaaa aaagtcgag gactttacc 
      gttatcagc aacaaatcg tggaaagac aaggccaat 
      aatttcgta caacagaaa agctactcg atcgatatt 
      cctgaaaat cccttcgtg gatattggg caagttggc 
      gacactgat aaattttta agtataagt aagaagtcg 
      cgccaacag aagctgtgt tcgtcgtcg ttgcaggat 
      ttgtcgagt tccacttcg agtatcaat agtggagtg 
      catgaatcg aacctggat ttgagtcac attgatccg 
      gacactcct attaagcat aaaaattgg aagtcgccg 
      gatgaggtc cggtttgga caagttgat agccaacgg 
      tttgaaagt aaagctaca agttacccc gatttgtcc 
      gattataaa tcgttcggg agcaaaagt aaaagtgtg 
      agtgaggag agattaaac gataaattg acggagtat 
      gagagactg gagcttttg aaattgttg cacgactgg 
      agcttgaat ggctcgtac tcgaaaagt gattttaat 
      atacgtttg acgaaatct agcgaacac ctggaaaag 
      gagaatctt gagacgtct gaaaagagt ctgaggcgg 
      agcagcgtt aaatttagt tccgagccg aatttatct 
      ccgaagttt gatattgtg atcgttaag tacagttcg 
      gaagataac atcaaagag aagaaggca actgatttc 
      gatttgatg cacaagtgc gagtttcgt aattgcatt 
      tttaacgaa gattttggt aaagttgcg aagccgaag 
      ggtattctg aagaacagt cgagaacgt ctccaggaa 
      accgagtgt cttaataat ctagcaaac caaaaaaca 
      ccaaaactg ggcccaaac tgtcgccgc tacagtgaa 
      ataatcgaa ccaaaatcg ttcaatagt caattagtt 
      cgttgcgat agcttagag cgcttaacc cacttgaac 
      aagaagtct ggccacaaa aaattcccc gactcttac 
      atagtcaca agacgcaat aaccaacgc aacaaaaac 
      tcgccaaaa aagtccccg aaaatcatc gtcctgaag 
      aagaaatac ctgccgaaa acgtggaaa agctgcagc 
      gacatcaag caccgcaaa accgtgcgc aaatgctgc 
      cgctacgcg aaaaaaaat tgccccatt ttgcgaaac 
      tcgtcggaa acgtcgcgg aaaacccag agttgcgtg 
      gacatcgaa cgggattac gctgctagg ttagctggg 
      gattcccaa gttggcgct tttgattgt cctcgacgc 
      atgtctgag cgggatttg ccatcgagg gtggactca 
      gaacgcaca attccctct tcaaagtcc gtgccagcg 
      cttcatcac atctcacca aactcggag caacctcgg 
      aacccacac gacgtcttt aacccgggt tatagtcgg 
      acctcgtcg aataatagc atcaatact aatagtgca 
      ccacctgcc aacggtcct gttaacaat caggcgggt 
      ttgcgcagc aggtctacg cacaatctg caccagcag 
      gatggggga ttttaccag aatctcagc gtttatagg 
      aacaaaatt cccagcccg caacaattg ggagacagg 
      acttccgcc cttcgaagt tctcaaaac tctctccaa 
      tcttcagta gtgaattcc caacgcccg gcttcggcc 
      tactaccct cccccgatg tctgtgaac gcctcacgt 
      tccaatctc caccaatca atcccgaat ttgaaaagt 
      cccccatcc acccatcga atccataac gaggagtcc 
      aggtccggc tcctaccca agcgtcaac caacaacac 
      catccccag aactacccg ggtccaaac cacccctat 
      tactacgac ccgaatcgt ccacctcaa gcacaaccc 
      atcacaaac taccctaat aacgtgcaa cacgtccac 
      tcgaacgga cagcgacaa gattataac aacgtcggt 
      ggtttactg cgtgaagac atggtaaga cagtcgcaa 
      aatgcccgc cacgaggag ataatgcgg tacttgagc 
      acaaataat gctcgaatt aacgaacaa aaccgaatt 
      gaagaaatg agagtttcg aaaagtgac gatctgttg 
      aagcatcac caagccaac gatttgatg aaatatgct 
      agcagtgga aacatcaga gcacacgag agtagcaaa 
      gtcgagatt cctctctac cggatgaaa gatgaagaa 
      gagttagat aaagatatg cgaatggcc aacaatgtg 
      aggtcgcaa aaattagtc gccaatgcg aaacaacaa 
      cgcgtaatg caataccaa catcccaac aaccaatac 
      tacgccccc taccacttc caaatggct caaaacaca 
      caaagtatg caaaatctc aacttgaac cagtaccaa 
      tattatccg acatccccg acatacaaa tcgcctccg 
      gtggccccc aagccgtcg aaacgacct gacgagcca 
      ccagaattg ccgccaaca agcacccac cctttgtac 
      tctgccagc agtcaagat ccaccgaag atggcattt 
      tatccgact tcgacggta ccgtcgacg aagacaccg 
      cgtgatcct tgggctaga gaggaacag gaacgtcaa 
      gctgaagca aggagggag gcttcgaga cagtggcag 
      gaacagcaa attcgggag ttgatggcg ttgccacag 
      aggacgcca cagcaggag gaacagttg agggtgttg 
      cagttggag agggagttt cagaggagg gcgatggag 
      gctgctgag caagatgac gatgatact gagaaggaa 
      agtccaagt ccgagtatg tcccagcct gtccagaac 
      cctccacct aagcaggaa ccgccccct tccatcctg 
      aaaccgggg gctgtaaac acccaaaca cagccgcct 
      ccagagcca gttgcgccg cccccgcct gaacgaggc 
      tctagtttc gccgtaatg tccatgcgg gcgaaggaa 
      aacacgaaa cgcgtgtcc ttcgacacg tcccctccg 
      caaccccct cccaacacc ttgcaactg gaggaaacc 
      gtcagagag gaccccaat agttttatt cgccaagct 
      gaatcgatg ttggcgtct ccgaccacc cccacggac 
      gcctccccc ggcgccgcc gtggccacc ccgggggtg 
      atcggggcc caggaggtg tacagggac cccagggcg 
      cgccgattg gccgaacaa gcgcagcag aaaaatcaa 
      acaatgcct ccggttcct gagaaattg tcatttaaa 
      gagaaaatg aaaatgttt gcgatggag accggcgag 
      caggaaact ccgaaggat aagagtaag ataagtagg 
      gcacaaaga gaaattgat aatttggga tcaccaagt 
      tcgggactt gttcattaa
    mRNA sequence
    • >TC033046 RA
      caccttgtg aagtgtgtg ttgcgagtt gcgaggacg 
      cggttgagg tgtgtaggt accgtttgg tgttttttt 
      caattaatc acccgaaaa aacaatctt gaaactatc 
      gcaaaagtg cgtgaacac gatgtaaaa gtgcgcagt 
      gcgtaaagt gcccagtgt gtgccttaa aaagtggcc 
      tttgagtga taaaataaa tgtttgcgt ttgtgagtg 
      ttgttttgg ccaggtctt gggataacc tacgacaac 
      aacagacgt agatgcatg gtaacgcca acgaccgca 
      gggccctcg aaagagagg ctctgcgaa atgttatca 
      accaatgga acgccaatc gactggatc tgttcgagt 
      tatccgaac cgaatgagg acttggaat tccacggtg 
      ttatgcgat tttacttcc aagactcgg ggcaaaaag 
      tcgccacca agtgcatac gagtggctt cggacgcca 
      ctgtggaag acgtgatcg gaactttgg tcgaaaaat 
      tccgacccg acatgagga tgttatcag tgccttcct 
      acgctctct acgaactcc atgagggat gccccgaac 
      gacgaatgc ttcccgacg agaaaccgc tccttgtgc 
      agctcaact ggcacgtgg acgaccgcg agggacggt 
      ttctcctca aaaatattg acgacaaaa cgaccaact 
      cggtcggtt ctctcgact cgaatgcga gttttcggc 
      gaaaattat cgaagcgag aaaagaagc agttgaaga 
      aacaggaaa aactctcga gaatgaaaa gcgaaaatg 
      gtgatatta acgataagg aagacggcg ttgcggaaa 
      aattataca cagaattgc ccgaaacgt cgttcacgc 
      gctcgattt cgaacccgg aggcggtga tgaggcggc 
      ggaggcaac aaaaactgg aaaagaaat tgcagcagt 
      ttaggtcga aggatggag ggcctgata cgggtggca 
      ctttgaaaa tctatggcg aggccttat gtaaagacg 
      tgccttaca aaaccctcc tcctctcag tgcgggatt 
      gcgctggtg ctgtggtcc gggaaatgt tggaaaaat 
      acggtctca acaaaaccg acccatcgc agtggtgtc 
      tcgtccaag tgacccaac aaccaggag tccctgaca 
      ctgaatacg tcctcgacg atgacgaat gccccttat 
      ctattctaa tgaactcgc caaacaccg gctccatca 
      tgtttcacg ttaggagac gaccggcag attgcgctc 
      cccgaaaaa gaaagaaaa aaccagggg cgctaggca 
      ccaacacca ataatccga gagatgttc gggatgctg 
      tgccccttt tggtcgaat tggggccag atgggtccg 
      ccccgcacc cgggggaca ccgccagga cgatccgac 
      tcacaaacg atgtcatgg aagtgggat cagcgaacg 
      gcatcgccc tgcagctct acggccccc acatccagc 
      cccggcatt gcgtcatca ccaacgccg acggggtga 
      catctttga caccttgtc atgcggacg cccatacct 
      acgttaatg gccagcgca tccatcaaa ccacaattt 
      taaaccatg gaagtcttg ttaaatttg gcaacagca 
      acacctatc gatttctag atccggttc acgaagaca 
      gaatgaggc atccagccg ttatggata atgccagga 
      tatacgata gccggtcgc cgcgagctc tcagtccta 
      ctgagccct ctcgggaaa atatcgaaa cgacgtttg 
      atctggaag gaaatgttg aaacgattt cgacggcca 
      gtggagcca gagaggata acaggtcgc tcggatcgg 
      catccgaaa ataggttga gcggcatgg accgctacc 
      cccgcggcc aggacccta tcctccccg ccgtgctgg 
      agttccccg aggaccccc aggagcagt tcctagctc 
      gcgttataa cgcatctcg acgtcacaa caccaacat 
      ttcgcctcg ccccagctt acactctat acctctgtg 
      cccgttatc gagcatcga ctcactacc gccccgaac 
      taacaccaa cagaacgtg cccacaaac tcaccgttt 
      tgcttcaac acgccgcca tgctgatcc gacacacgg 
      tccaagacc gaagcaccg aaagcatat ctcaagctt 
      tctggttag ccaacgcca gtgaattac tacacttcc 
      tcaaatcgg acagacacg tcagtgcgt tctcgatcc 
      aagcacaag acacacttg cagatgcag tacaattag 
      cttttaaga atttagtcg cgtgtttaa ccgacgagt 
      taaactcgg ccatggctc atttgatgt cggtgacag 
      gggatgacc atccccgcg aagttataa atattttaa 
      gtggtgcta tgaatctcc tacggaagt gtcgggtca 
      atgcggccc taaccatcc aattatttt cccagttgt 
      tccactgga tttcggcca aagcgctcg caaatgtga 
      tcagtaaca gtaatttat gtacgagaa gtttcggca 
      ataaactct tcaatcgat taaggaacc tgcaagctt 
      gggctgagt cccaaggct tggagttgg ctgcggagt 
      gccaccttg ccaagatcg tgcaatgtg cacacttga 
      tgcaagcac ctaaataca cgccggagg atttagcca 
      acttgtcgt cggcctgtt tcaaattaa attcgatgc 
      aattggggg ctttattaa gacagtaca aggcggaac 
      ctggggaga aattggcac cgcccccgt tgctggagc 
      aggcggcca aagcggccg aaagcgtcg ccgacgagc 
      ttgcgcggg ctgaaggtc gcgaagtca cactttacg 
      aagacccag cgccaccac tacaacttt tactccccg 
      atgatgggt ttagctgcg atgtcgttc aaggggttc 
      caccaggtc ttgccgact tcctgcacc cgctgcaag 
      cgtcgggtt tgtgtcgtc tggccgcgc aaccgacga 
      gttcgggcc attggacag tgtacatgt ccgcgccac 
      ggcctccca gtgctttga gtacgaccc aaccagagg 
      tccaagtca ttcggttgc ataaggctg ggggtggca 
      tgggcttgt cgatagtgg cagccaaag gagccggtc 
      aggagcgac tcggaattt atataaaat cggtggttc 
      ccggaggag ctgcagatc gagatgggc gattggctg 
      ctggcgatc aactactat cagtcgatg gacaatccc 
      ttctgggaa tcacacaag aaaaagccg ccgagtttc 
      tggtacgga ccggaagtg ttgtcacac ttgaagtag 
      cgaaagggg gtgctgtgt atcacggat tggccacat 
      tgcttcaac agccgagtc ctacacctc atcgagctg 
      gggatttct acgctgaat cctactaca gctcgcaag 
      agtccctct acagcctaa acaattgct tggtgttca 
      cgcccccgg ccaccaaac ccaaaaaca acgtatttt 
      tcagctccg atgacactc tttacaaaa aagaaacgc 
      gcaaattca ccatccctc gaatcgaag tcgagagtg 
      acgaacccg ttttcaatc tacaagtaa ccgattttt 
      ccgaaccat cccccaaaa acattattc tggggtacg 
      acgaaacgg gtaccaact ttgaaacga aaaaagtcg 
      aggacttta ccgttatca gcaacaaat cgtggaaag 
      acaaggcca ataatttcg tacaacaga aaagctact 
      cgatcgata ttcctgaaa atcccttcg tggatattg 
      ggcaagttg gcgacactg ataaatttt taagtataa 
      gtaagaagt cgcgccaac agaagctgt gttcgtcgt 
      cgttgcagg atttgtcga gttccactt cgagtatca 
      atagtggag tgcatgaat cgaacctgg atttgagtc 
      acattgatc cggacactc ctattaagc ataaaaatt 
      ggaagtcgc cggatgagg tccggtttg gacaagttg 
      atagccaac ggtttgaaa gtaaagcta caagttacc 
      ccgatttgt ccgattata aatcgttcg ggagcaaaa 
      gtaaaagtg tgagtgagg agagattaa acgataaat 
      tgacggagt atgagagac tggagcttt tgaaattgt 
      tgcacgact ggagcttga atggctcgt actcgaaaa 
      gtgatttta atatacgtt tgacgaaat ctagcgaac 
      acctggaaa aggagaatc ttgagacgt ctgaaaaga 
      gtctgaggc ggagcagcg ttaaattta gttccgagc 
      cgaatttat ctccgaagt ttgatattg tgatcgtta 
      agtacagtt cggaagata acatcaaag agaagaagg 
      caactgatt tcgatttga tgcacaagt gcgagtttc 
      gtaattgca tttttaacg aagattttg gtaaagttg 
      cgaagccga agggtattc tgaagaaca gtcgagaac 
      gtctccagg aaaccgagt gtcttaata atctagcaa 
      accaaaaaa caccaaaac tgggcccaa actgtcgcc 
      gctacagtg aaataatcg aaccaaaat cgttcaata 
      gtcaattag ttcgttgcg atagcttag agcgcttaa 
      cccacttga acaagaagt ctggccaca aaaaattcc 
      ccgactctt acatagtca caagacgca ataaccaac 
      gcaacaaaa actcgccaa aaaagtccc cgaaaatca 
      tcgtcctga agaagaaat acctgccga aaacgtgga 
      aaagctgca gcgacatca agcaccgca aaaccgtgc 
      gcaaatgct gccgctacg cgaaaaaaa attgcccca 
      ttttgcgaa actcgtcgg aaacgtcgc ggaaaaccc 
      agagttgcg tggacatcg aacgggatt acgctgcta 
      ggttagctg gggattccc aagttggcg cttttgatt 
      gtcctcgac gcatgtctg agcgggatt tgccatcga 
      gggtggact cagaacgca caattccct cttcaaagt 
      ccgtgccag cgcttcatc acatctcac caaactcgg 
      agcaacctc ggaacccac acgacgtct ttaacccgg 
      gttatagtc ggacctcgt cgaataata gcatcaata 
      ctaatagtg caccacctg ccaacggtc ctgttaaca 
      atcaggcgg gtttgcgca gcaggtcta cgcacaatc 
      tgcaccagc aggatgggg gattttacc agaatctca 
      gcgtttata ggaacaaaa ttcccagcc cgcaacaat 
      tgggagaca ggacttccg cccttcgaa gttctcaaa 
      actctctcc aatcttcag tagtgaatt cccaacgcc 
      cggcttcgg cctactacc ctcccccga tgtctgtga 
      acgcctcac gttccaatc tccaccaat caatcccga 
      atttgaaaa gtcccccat ccacccatc gaatccata 
      acgaggagt ccaggtccg gctcctacc caagcgtca 
      accaacaac accatcccc agaactacc cgggtccaa 
      accacccct attactacg acccgaatc gtccacctc 
      aagcacaac ccatcacaa actacccta ataacgtgc 
      aacacgtcc actcgaacg gacagcgac aagattata 
      acaacgtcg gtggtttac tgcgtgaag acatggtaa 
      gacagtcgc aaaatgccc gccacgagg agataatgc 
      ggtacttga gcacaaata atgctcgaa ttaacgaac 
      aaaaccgaa ttgaagaaa tgagagttt cgaaaagtg 
      acgatctgt tgaagcatc accaagcca acgatttga 
      tgaaatatg ctagcagtg gaaacatca gagcacacg 
      agagtagca aagtcgaga ttcctctct accggatga 
      aagatgaag aagagttag ataaagata tgcgaatgg 
      ccaacaatg tgaggtcgc aaaaattag tcgccaatg 
      cgaaacaac aacgcgtaa tgcaatacc aacatccca 
      acaaccaat actacgccc cctaccact tccaaatgg 
      ctcaaaaca cacaaagta tgcaaaatc tcaacttga 
      accagtacc aatattatc cgacatccc cgacataca 
      aatcgcctc cggtggccc ccaagccgt cgaaacgac 
      ctgacgagc caccagaat tgccgccaa caagcaccc 
      accctttgt actctgcca gcagtcaag atccaccga 
      agatggcat tttatccga cttcgacgg taccgtcga 
      cgaagacac cgcgtgatc cttgggcta gagaggaac 
      aggaacgtc aagctgaag caaggaggg aggcttcga 
      gacagtggc aggaacagc aaattcggg agttgatgg 
      cgttgccac agaggacgc cacagcagg aggaacagt 
      tgagggtgt tgcagttgg agagggagt ttcagagga 
      gggcgatgg aggctgctg agcaagatg acgatgata 
      ctgagaagg aaagtccaa gtccgagta tgtcccagc 
      ctgtccaga accctccac ctaagcagg aaccgcccc 
      cttccatcc tgaaaccgg gggctgtaa acacccaaa 
      cacagccgc ctccagagc cagttgcgc cgcccccgc 
      ctgaacgag gctctagtt tcgccgtaa tgtccatgc 
      gggcgaagg aaaacacga aacgcgtgt ccttcgaca 
      cgtcccctc cgcaacccc ctcccaaca ccttgcaac 
      tggaggaaa ccgtcagag aggacccca atagtttta 
      ttcgccaag ctgaatcga tgttggcgt ctccgacca 
      cccccacgg acgcctccc ccggcgccg ccgtggcca 
      ccccggggg tgatcgggg cccaggagg tgtacaggg 
      accccaggg cgcgccgat tggccgaac aagcgcagc 
      agaaaaatc aaacaatgc ctccggttc ctgagaaat 
      tgtcattta aagagaaaa tgaaaatgt ttgcgatgg 
      agaccggcg agcaggaaa ctccgaagg ataagagta 
      agataagta gggcacaaa gagaaattg ataatttgg 
      gatcaccaa gttcgggac ttgttcatt aatacttgt 
      tacttgttg aatgtgtga tagatttaa agaaccaaa 
      atgaccaaa tatacaaga tattgttag tggaaattt 
      attacaatg ttgtcagtc ctgctaatt tatcttgca 
      tatctaact gtaccgtcc aacctgcag ctaattatg 
      cgtctacaa cgactaagt gtattgatt gattttaaa 
      cgtagtggc ttatgacgt gattcaatt gaaatgaga 
      tggtttttg tttgataga gaactttat tcaaatata 
      tattgtatc atattttag agatcctat tttcgtcat 
      tttatatat aaattattt ttcctagag tttagataa 
      ccatgatat tgtaaaagc aatacttgc atataagga 
      ccaatgtta tacatacac tcacacttt caattctct 
      ctacattcc ttctttcgt tacagcttt cgttcgttt 
      gaggcaata aatgttcat atttacatt caaatttta 
      tgacgtagt gtatataaa atatgtaaa ttgtcatca 
      aaagtagca gtggaattc attagattg tcatattaa 
      atagagtat aatatagac aaaattctg ttttatttc 
      tttatttta tgtactatg aataaacaa tatacatat 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY