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Gene information
General information
TC013996 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3739181..3751526
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC013996
      iBeetle screen iB_05539

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC013996 RA
        MNCCNYMQY RHHQPYYGS HYYEPMPYH GDFYGQQLY 
        QDYYGTTPY YHQQWPCAS SSFPDYVYN PKEARIRKA 
        MRDASRERS IAGTSPMLA RPNARRAPS HGWGAPSHD 
        DHLMMSPLG GMQPPSCSF AMSGGGDQF MGGGISAYN 
        KLEAAAAPP PPPPPSAGS VHTNYLQSN PLAQLQQHA 
        EMWPDYHTM RSRSSGESF MMGDVAPYS GQTPEMGPI 
        MLDKQPTPA TSPPTAAPD KEPDQDQFI PVFEQPEEA 
        KAKNKASAC QKPASKPLP DFNEAFGST ERGRFQSPP 
        DPRLAPNKG YLDYFFSDS DLIKFDDYQ M
      CDS sequence
      • >TC013996 RA
        atgaattgc tgcaattac atgcagtac agacaccac 
        cagccgtat tacgggtcc cactactac gagcccatg 
        ccctaccat ggcgatttc tatgggcag caactctac 
        caagactac tacggcacg accccctat taccaccag 
        caatggcca tgtgcctcc tcctccttc ccagattac 
        gtttacaac ccgaaggag gcgagaata cgcaaagca 
        atgcgcgat gcaagccgg gaacgttcg atcgccggg 
        acttccccc atgcttgcg agacccaac gcgcgcaga 
        gctccttca cacggctgg ggggcacct tcgcacgac 
        gaccacctc atgatgtcg cctctggga gggatgcag 
        cccccgtct tgcagtttt gcgatgtcg ggaggcggc 
        gaccagttc atgggaggc ggcataagc gcctacaac 
        aaactggaa gcggcggcg gcgccgcca ccaccaccg 
        ccgccgtcg gccggttcg gtgcacacg aactacctg 
        cagtcgaat ccgttggca caactgcaa cagcatgct 
        gagatgtgg cccgactat cacacgatg cgctcgaga 
        tcttcaggt gaatcgttt atgatgggt gatgtcgct 
        ccttactcc gggcagact ccggagatg ggcccaatc 
        atgctggac aagcagccg acgcccgcg acgtctccg 
        cccaccgcc gctccggac aaggagccg gaccaggac 
        caattcatt ccggtcttt gaacaaccg gaagaggcc 
        aaggcgaaa aataaggcg tcggcgtgc caaaagccc 
        gcgagcaaa cctctgccg gacttcaac gaagcgttc 
        ggatccaca gaacggggg aggtttcag agtccgccc 
        gatccgcgt ctggcgccc aacaagggt tatctcgat 
        tatttcttc agcgacagc gacctgatc aaattcgac 
        gattatcaa atgtaa
      mRNA sequence
      • >TC013996 RA
        gaggtgagg cgtcgcggc gcgtccacg tattgattg 
        tggcgttgg ttcagtcgg cgacggcgt ccggcgtcg 
        caaactgcg cgtctccgc cgtcgtagg tggctcccg 
        ttgttcagt tcgtggcga ttttggcgc gaaaccaga 
        agctcgccc gcgatggct ctcacctag tgccaaaat 
        cgataaaat atccctaaa cactactac tgagcatga 
        attgctgca attacatgc agtacagac accaccagc 
        cgtattacg ggtcccact actacgagc ccatgccct 
        accatggcg atttctatg ggcagcaac tctaccaag 
        actactacg gcacgaccc cctattacc accagcaat 
        ggccatgtg cctcctcct ccttcccag attacgttt 
        acaacccga aggaggcga gaatacgca aagcaatgc 
        gcgatgcaa gccgggaac gttcgatcg ccgggactt 
        cccccatgc ttgcgagac ccaacgcgc gcagagctc 
        cttcacacg gctgggggg caccttcgc acgacgacc 
        acctcatga tgtcgcctc tgggaggga tgcagcccc 
        cgtcttgca gttttgcga tgtcgggag gcggcgacc 
        agttcatgg gaggcggca taagcgcct acaacaaac 
        tggaagcgg cggcggcgc cgccaccac caccgccgc 
        cgtcggccg gttcggtgc acacgaact acctgcagt 
        cgaatccgt tggcacaac tgcaacagc atgctgaga 
        tgtggcccg actatcaca cgatgcgct cgagatctt 
        caggtgaat cgtttatga tgggtgatg tcgctcctt 
        actccgggc agactccgg agatgggcc caatcatgc 
        tggacaagc agccgacgc ccgcgacgt ctccgccca 
        ccgccgctc cggacaagg agccggacc aggaccaat 
        tcattccgg tctttgaac aaccggaag aggccaagg 
        cgaaaaata aggcgtcgg cgtgccaaa agcccgcga 
        gcaaacctc tgccggact tcaacgaag cgttcggat 
        ccacagaac gggggaggt ttcagagtc cgcccgatc 
        cgcgtctgg cgcccaaca agggttatc tcgattatt 
        tcttcagcg acagcgacc tgatcaaat tcgacgatt 
        atcaaatgt aaaaattgt ttcggctca atttagcaa 
        aattttgga attgtgcct ttttgtttc ggttttttc 
        gttgggacc aagaaactt tattccggt tgtagaggc 
        gaatgttca gcactatat tctagtcaa atgtttgta 
        tatataaaa agttagctg ttaatatgt catgtaacc 
        catttaaag taatccgtg ttaaagaat aaaggtcgg 
        aggattatt gattgttac gtttggtag gtttgagga 
        gagtgaggt tttcgatcg gagcgtcta gaatcaccc 
        taatatcac ttaggttat aaatgtaca gaattgtaa 
        atgtattta gtgttaaag taaggtcgt gccttagta 
        gataattca aaatttaac aaaaaaaca tcaacaatt 
        agagcttcc cgtcgtgta tttgggaga attttgtaa 
        ataaaattt taatccaaa gaatatgct tcccaaagg 
        tttttttga gaatagcga catattcta aatcttcct 
        ttagtcttc aaaatcaag cgaattggt tgcgaatca 
        aacaacaga atcagacca gaattctca aacgaatta 
        atcaaatcg ttttgtact agattaatg tggtccatt 
        ctttatatc tatacttgt gatcgtcat agctagcgt 
        aataatcaa accaaaaat tcccccaaa aaaatggaa 
        caacaccag attatattg tacaaatat attaaagtt 
        gaatattcc cattatatt ggcggccga ggaatcgca 
        aacaacttc aaacaccga cttgcaaaa atccgtctc 
        actatccaa acagtacaa ttacgaaac aagacaaaa 
        acagcgttg ttttcggtc ccgcgccgc cctatattg 
        tatgttgta tttaggatt atttctagc agtcttaga 
        gcctgggga gtccccgct tccattatt gtgttaaat 
        ccatatcaa agtgatatc gttcaatta caaaatata 
        taatttaga gctgacttt gttttttag tcactttcc 
        ggtgaaatc tttctcccc ctatcctcg caaagatta 
        cgagcgtcg cctaatctc ttcagcaac gacttggag 
        tatcaaagc aactacatc ctcgagtac ttccagagc 
        atgccgtcg atgacccta atgaacatc accactccc 
        aacgggacc acttttccc tatgctacc tacacaccg 
        tccgattta atcgaaaac atcaacaac gaggcgcaa 
        gtcttcgaa aacgtcacc gaaccgagc tttttccac 
        acggatgta ggcttcgta cccctctca tccgtgtaa 
        tctttgtat tgtatttaa ttgttgaat aaagtgaaa 
        acaagttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY